Product datasheet
Anti-ATP citrate lyase antibody ab133403
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Overview
Product name
Anti-ATP citrate lyase antibody
Description
Rabbit polyclonal to ATP citrate lyase
Tested applications
WB
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of
Human ATP citrate lyase.Read Abcam's proprietary immunogen policy
Positive control
This antibody gave a positive signal in Human Brain and Human Testis tissue lysates as well as
the following whole cell lysates: HepG2; HEK293; PC3.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a
stabilising agent. If you would like information about the formulation of a specific lot, please
contact our scientific support team who will be happy to help.
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab133403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 121 kDa
(predicted molecular weight: 121 kDa).
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Target
Function
ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in
many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in
the biosynthesis of acetylcholine.
Sequence similarities
In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
Contains 1 ATP-grasp domain.
Post-translational
modifications
ISGylated.
Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by
glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites.
Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation.
Ubiquitination is probably inhibited by acetylation at same site.
Cellular localization
Cytoplasm.
Anti-ATP citrate lyase antibody images
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All lanes : Anti-ATP citrate lyase antibody
(ab133403) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult
normal tissue (ab29466)
Lane 2 : Testis (Human) Tissue Lysate - adult
normal tissue (ab30257)
Lane 3 : PC3 (Human prostate carcinoma
cell line) Whole Cell Lysate Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver
carcinoma cell line) Whole Cell Lysate
Western blot - Anti-ATP citrate lyase antibody
Lane 5 : HEK293 (Human embryonic kidney
(ab133403)
cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP)
preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 121 kDa
Observed band size : 121 kDa
Additional bands at : 19 kDa,39 kDa,70
kDa. We are unsure as to the identity of these
extra bands.
Exposure time : 4 minutes
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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