Product datasheet
Anti-AMPK beta 1 antibody [EP345] ab108250
2 Images
Overview
Product name
Anti-AMPK beta 1 antibody [EP345]
Description
Rabbit monoclonal [EP345] to AMPK beta 1
Tested applications
WB, Flow Cyt
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues in Human AMPK beta 1
Positive control
293T, fetal kidney, Hela or NIH/3T3 lysate Permeabilized HeLa cells
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP345
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab108250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular
weight: 38 kDa).
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Application
Abreviews
Flow Cyt
Notes
1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype
control with this antibody.
Application notes
Is unsuitable for ICC,IHC-P or IP.
Target
Function
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase
that plays a key role in regulating cellular energy metabolism. In response to reduction of
intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energyconsuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell
growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by
longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of
cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin.
Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its Cterminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2
or PRKAG3).
Sequence similarities
Belongs to the 5'-AMP-activated protein kinase beta subunit family.
Domain
The glycogen-binding domain may target AMPK to glycogen so that other factors like glycogenbound debranching enzyme or protein phosphatases can directly affect AMPK activity.
Post-translational
modifications
Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2).
Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the
existence of a regulatory feedback loop between ULK1 and AMPK.
Anti-AMPK beta 1 antibody [EP345] images
All lanes : Anti-AMPK beta 1 antibody
[EP345] (ab108250) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Fetal kidney lysate
Lane 3 : HeLa cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Western blot - AMPK beta 1 antibody [EP345]
HRP labelled goat anti-rabbit at 2000
(ab108250)
Predicted band size : 38 kDa
Observed band size : 38 kDa
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Flow cytometric analysis of permeabilized
HeLa cells using ab108250 at a 1/10 dilution
(red), or a rabbit IgG (negative) (green).
Flow Cytometry - AMPK beta 1 antibody [EP345]
(ab108250)
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