Corey Moffet, USDA-ARS, U.S. Sheep Experiment Station, Dubois, ID Determine the effect of timing of herbicide application on efficacy and subsequent reseeding success The study was initiated in 2008 • Large plot study (Aberdeen, ID) Crested wheatgrass control treatments and reseeding success • Small plot study (Dubois, ID) Crested wheatgrass TNC trends and phenology Herbicide efficacy Site Description • Near Aberdeen, ID 33 ha area • Loamy 8-12 Wyoming big sagebrush/ bluebunch wheatgrass site • Seeded to crested wheatgrass in 1996 after wildfire Main plots (80) • Disked in spring or not • Glyphosate application (3.2 l/ha None, vegetative stage, reproductive stage, or fall regrowth • Control treatment/seeding year 2008 or 2009 • 5 reps Subplots (160) • Half the main plot seeded the other half not Repeated measurements • 1 and 2 years after seeding Drill mix Anatone bluebunch wheatgrass Magnar basin wildrye Bannock thickspike wheatgrass Thurber’s needlegrass Broadcast mix Maple Grove Lewis flax Mtn. Home Sandberg bluegrass Hotrock penstemon Wyoming big sagebrush Rubber rabbitbrush * The % 40 10 10 10 Full Rate 8 8 6 6 5 5 5 10 5 8* 4* 8* 0.5* 3* #PLS/ ac 3.20 0.80 0.60 0.60 0.40 0.20 0.40 0.05 0.15 PLS/linear ft. 20 5 4 5 5 9 5 4 5 broadcast seeding rate for each component is double the standard full seeding rate for drilling Line point intercept • 5 lines 8, 15, 22, 29, & 36m • 20 points/line Every m between 6 and 25 Density • Abundant species 20 quadrats, 0.04 m2 • Common species 20 quadrats, 1 m2 • Rare species Belt transect U.S. Sheep Experiment Station Herbicide application plots Plants sampled for TNC trend June 6, 2008 Timing of herbicide application and clipping • Main plot Herbicide application date • Split plot Half clipped 2 wks before herbicide Total Nonstructural Carbohydrate (TNC) • 20 plants sampled on alternate wks • 10 clipped 2 wks prior to sampling May 22, 2008 - Clipped June 22, 2008 June 6, 2008 - Sprayed Summary Determine whether herbicide is more efficacious on crested wheatgrass if it is applied when photosynthate is being translocated to crown meristems. • Determine whether clipping can be used to increase the carbohydrate sink Determine whether differences in crested wheatgrass control translates into greater reseeding success