AST 248 HW for Chapter 6 Ryan Richards Review Questions What are the three lines of fossil evidence that point to an early origin of life on Earth? Discuss each line and what it tells us about when life arose. What are the implications of an early origin for the possibility of life elsewhere? 1) Stromatolites: Rocks characterized by a distinct layered structure. The book mentions that the ancient stromatolites look similar to the microbes today. The microbes on the top later of these rocks are photosynthetic. Their structural similarities imply that they are fossil remnants of early life. 2) Microfossils: These are fossilized cells. The oldest fossils have great controversy since they have undergone geological processes over time. Therefore it is difficult to differentiate what are cells and what aren’t. The younger microfossils have been shown to contain molecules like hydrocarbons. They can conclude that there was life before about 3.0 billion years ago. 3) Isotopic Evidence: By observing the amount of carbon-12 and carbon-13, it has been shown that fossils of living organisms have a slightly lower fraction of carbon-13 in them than inorganic material do. Also the rocks should have been sedimentary before undergoing transformations. Life also affects the isotopic ratios of other elements such as sulfur, iron and nitrogen. How do DNA sequences allow us to reconstruct the evolutionary history of life? What living organisms appear to be most closely related to the common ancestor of all present life? DNA sequences allow us to reconstruct the evolutionary history of life. By looking and comparing DNA sequences, we can observe similarities which imply that two organisms came from the same common ancestor. The closest relatives of the universal ancestor of all life are the simple single-cell organisms. What was the Miller-Urey experiment, and how did it work? Why is the relevance now subject to scientific debate? How else might earth have obtained the organic building blocks of life? The Miller-Urey experiment was an experiment testing the idea that the earth’s early atmosphere was oxygen free. It suggested that the chemical reactions fueled by sunlight could have led to the creation of organic molecules. They used small glass flask to simulate chemical conditions of the early Earth. One was partially filled with water to represent the sea and heated to produce vapor. They added ammonia and methane gas to the vapor which modeled the atmosphere. The gases were transported to a second flask, where electric sparks produced chemical reactions. The gas was then cooled for condensation and the resulting liquid turned brown and chemical analysis showed there were amino acids and other organic molecules. The relevance is subject to debate since ammonia and methane were not present in the Earth’s early atmosphere. Scientists believe that hydrogen can play a role in facilitating the development of organic molecules. It is unknown whether hydrogen was present in the early atmosphere. The other sources of organic building blocks are from reactions near deep sea vents and material from space such as meteorites. What do we mean by an “RNA world”, and why do scientists suggest that such a world preceded the current “DNA world”? An RNA world is a world where genetic information is stored in RNA instead of DNA. Scientists believe this preceded the current DNA world since RNA is a simpler molecule than DNA. For example, RNA is single stranded and DNA is double stranded with a backbone structure. Some may know from biology how complicated it is to make DNA. Briefly summarize current ideas about the sequence of events through which life may have originated on Earth. What role(s) might clay or other inorganic materials have played? 1) The formation of amino acids and other organic molecules 2) Larger organic molecules like RNA formed from smaller organic molecules. These reactions could have taken place through inorganic molecules like clay. Pre-cells enclose RNA strands 3) Self-replicating RNA forms inside pre-cells and natural selection takes over. 4) Natural selection continues until complex organism form. 5) DNA evolves from RNA and dominates. DNA becomes the basic unit of encoding genetic information. Briefly describe the possibility that life migrated to Earth. Also discuss the possibility that Earth life might have migrated to other worlds, and the implications of migration to the search for life elsewhere. Due to the impacts of meteorites, surviving microbes on these meteorites can travel to another planet after it had hit the Earth. We can deduce that microbes from the Earth may have traveled to nearby planets. However, it would be very difficult to determine whether or not life arose from the Earth or from a nearby planet. Why do we think that evolution would have proceeded rapidly at first, and what fossil evidence supports this conclusion? The reason is because the simple organisms back then reproduce very quickly. Also the lack of mutations made things simpler as well. The fossil evidence supporting this conclusion came from photosynthetic organisms. This implies that photosynthesis occurred rapidly if there was rapid evolution. How do we think that eukaryotes evolved? What time constraints can we place on when eukaryotes first got cell nuclei? We think that eukaryotes evolved by bacteria engulfing one another. For example, this might explain why mitochondria has DNA as well (its circular like we find in prokaryotes). The two bacteria form a symbiotic relationship (simply they help each other out) which eventually causes them to form into one organism. There are fossils that go back 2.1 billion years showing that eukaryotes had cell nuclei. What was the Cambrian explosion? Briefly discuss ideas about what might have caused it and why no similar event has happened since. The Cambrian explosion occurred between 540 and 500 million years ago. During this period we began to see a diversification of life on Earth. Although there is no definitive answer to the cause, there are some key ideas on what may have caused it. These factors include the presence of oxygen, the evolution of genetic complexity, climate change and the absence of predators. The last of these ideas is why we haven’t seen a period like the Cambrian since. Summarize the history of oxygen buildup as it is understood today, and describe key mysteries that still remain. When did oxygen reach current levels? There was an assumption made that cyanobacteria began producing oxygen at 2.7 billion years ago. There was also a hypothesis that rock and ocean minerals oxidized which removed some of the oxygen in the atmosphere as rapidly as the cyanobacteria produced it. After the oxidation could the atmosphere build up. The concentration of oxygen began to increase about 2.35 billion years ago which would then be able to support complex life. Oxygen reached its current level about 1 billion years ago. The mystery comes from the fact that it a long time for the oxygen to build up in the atmosphere possibly due to the oxidation in rocks and ocean materials as mentioned aboved. Would You Believe It We discover evidence of life, in the form of a particular ratio of carbon-12 to carbon-13 in rock that was originally formed in sediments and is 3.9 billion years old. This one is interesting. It is possible however difficult to say since the sediments can’t be date for rocks older than 3.85 years old as mentioned in the book. We discover an intact fossil of a eukaryotic cell, with a cell nucleus that is 3.0 billion years old. This is possible considering the earliest and oldest fossils of eukaryotic cells have been shown to have nuclei as far as 2.1 billion years ago. The book also mentions that these cells could have had cell nuclei earlier but they haven’t been able to recognize them in their fossil records. We discover a preserved, 3.5-billion-year-old microfossil that apparently had a genome genetically just like that of many modern animals. This is an interesting one. One can argue for this or against this. There has been evidence of early life in microfossils. We discover clear evidence that life arose on a high mountaintop, not in the oceans. I don’t believe this. Early life of mountaintops were not suitable for life. We discover a fossil of a large dinosaur that lived approximately 750 million years ago. This is unlikely. The mass extinctions would have eradicated any fossils. Also dinosaurs arose later than that. The first animals however did arise around this period. We discover that, contrary to present belief, oxygen was abundant in Earth’s atmosphere at the time when life arose. This is not believable. The book says oxygen would break up organic bonds. Therefore, oxygen would have been detrimental to early life on Earth. We discover a crater from the impact of a 10-kilometer asteroid that dates to about 2500 years ago. This is unlikely since an asteroid of that size would have destroyed the Earth. We discover an asteroid about 3 kilometers across that is on a collision course with Earth. This is believable. We find fossil remains of an early primate that lived about 50 million years ago and was, from all appearance, identical to a modern gorilla. This is not believable. The earliest primates came before this time and there an extremely small chance that they looked like gorilla. Also gorillas diverged from monkeys much later 50 million years ago. The first life created in the laboratory has an RNA genome, rather than a DNA genome. This is plausible considering early life consisted of RNA. RNA is simpler than DNA and therefore much easier to recreate in a lab setting. Quick Quiz Which statement about Earth’s ozone is true? (a) It formed only after the atmosphere became rich in oxygen. The hypothesis that an impact killed the dinosaurs seems (a) well supported by geological evidence According to the fossil evidence, modern humans (b) evolved on a lineage that split from other apes 6 million years ago or more Quantitative Problems Bacterial Evolution. Suppose that a mutation occurs in about 1 of every 1 million bacterial cells, and suppose that you a bacterial colony in a bottle like that described in Cosmic Calculations 6.1 (in which the bacteria divide every minute). Given the number of bacteria in the bottle after 1 hour, approximately how many bacteria would have some type of mutation? What does this tell you about why bacteria often evolve resistance to new drugs? We begin by considering only one bacterium in the bottle. Then after one minute we have two bacteria. After two minutes we have four and after three minutes we have eight. The evident generalization of this 2t, where t is the number of minutes. After one hour or 60 minutes, there are 1 million trillion bacteria or 1 x 1018 (which is given in Cosmic Calculations 6.1). We know that 1 out of every million bacteria have mutations; therefore the ratio is 1/106 or 10-6. We take this ratio and multiply it by the number of bacteria to get the number of bacteria with mutations. The answer is 1012 bacteria. From this answer we see that there are more bacteria with mutations than there are without. As a result, we see that this large number can have different mutations which would help bacteria resist new drugs. Please note, the answer is not three bacteria like some may have thought, remember we are subtracting exponents.