D A T A
Lenntech
S H E E T
info@lenntech.com Tel. +31-152-610-900
www.lenntech.com Fax. +31-152-616-289
Biomax Membranes
TM
The membrane of choice for fast processing, and exceptional
chemical resistance
Conventional UF membranes cast with
macrovoids have tighter average pore
sizes and must operate with reduced
flux to keep retention high.
The high flux and high retention
properties of the Biomax membrane
result in faster processing speeds with
higher yields, which means shortened
processing times and a bioprocessing
system that can be smaller and more
compact.
Biomax membranes are composed
of polyethersulfone and are resistant to
harsh chemicals used in cleaning,
biological decontamination, and
sanitization. The polyethersulfone
Biomax membrane has been modified
to reduce non-specific protein binding
compared to conventional polyethersulfone membranes.
Biomax. Composite Polyethersulfone with void-free structure
Biomax membranes are the membranes of choice for applications
requiring high flux, low to moderate
protein binding, and harsh chemical
cleaning and/or sanitization.
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Advantages of Choosing
Biomax Membranes
• Void-free structure results in high
flux, excellent retention and
higher yields.
Typical Applications
• Composite polyethersulfone
membrane provides a stable
hydraulic environment, resulting
in excellent mechanical strength
and integrity.
• Concentration, buffer exchange,
and depyrogenation of protein
solutions containing biomolecules
such as albumin, IgG, IgM,
monoclonal antibodies, hormones,
and growth factors.
• Polyethersulfone membrane has
superior resistance to harsh
cleaning chemicals with no
degradation of processing
performance through multiple
cleaning cycles.
• Harvest, clarification, and
concentration of vaccines.
The more open average pore size
permitted by the void-free structure of
the Biomax membrane results in higher
fluxes with maximum retention.
• Biomax membranes are available
in a wide range of molecular
weight cut-offs to meet all of your
application needs.
100
Tighter Retention Profile
80
Retention %
The retention profile of Biomax 10 kDa
membrane is much sharper than that
of a conventional 10 kDa membrane,
translating into improved protein
retention in your process stream
(Figure 1).
Conventional 10k
HAS-68
kDa
60
40
20
Biomax 10
0
0
10000
100000
Molecular Weight
Figure 1. Protein retention of Biomax membrane versus
conventional Polyethersulfone UF membrane
Improved Integrity
50
The void-free structure of the Biomax
membrane significantly reduces the
incidence of microdefects, resulting in
improved membrane integrity
(Figure 2).
45
Conventional Cassettes
Polyethersulfone
30kD Modified
10kD
Polyethersulfone
10kD Modified
Polyethersulfone
30kD
Polyethersulfone
Air Flow (std cc/min)
40
35
30
Pellicon-2 Cassettes with
Biomax-10 Membrane
25
20
15
10
5
0
0
10
20
30
40
50
60
70
80
90
Air Pressure (psi)
Figure 2. Integrity testing of Biomax membranes versus
conventional Polyethersulfone UF membranes
Improved Process Yields
You can decrease the size of the
system and improve your yield, thereby
reducing your overall processing costs
(Table 1).
Parameter
Retention (%)
Flux (lmh)
Recirculation rate (lpm)
Pipe diameter (inches)
Hold-up volume (liters)
Yield improvement (%)
Table 1.
Biomax 10
Conventional
Polyethersulfone (10 kDa)
99.95
118.0
4.0
1.5
8.4
2–3
99.9
80.0
6.0
2.5
20.8
—
120
Superior Flux
At working concentrations of protein,
Biomax membranes have higher flux
for a given protein retention than
conventional polyethersulfone UF
membranes. In this example, Biomax
10 membrane demonstrates a 40%
improvement in process flux over a
conventional 10 kDa polyethersulfone
membrane using 10% BSA (Figure 3).
Biomax 10 with A screen
100
Conventional 10K
Protein = BSA
Qfeed = 3 lpm
TMP = 40 psi
Temp = 8 °C
Flux (mh)
80
60
40
20
0
0
5
15
10
20
25
Concentration (%)
Figure 3. High flux of Biomax membrane versus
conventional Polyethersulfone UF membrane
Before
Pre-Run 1
99.8
2
3
4
5
6
7
8
9
200
Flux (lmh-bar at 15 °C)
99.7
99.6
99.5
99.4
150
100
99.3
99.2
50
99.1
0
99
Biomax
10
Example 1
Biomax
10
Example 2
Results
Air integrity (sccm) prior to exposure
BSA retention % prior to exposure
Air integrity (sccm) after exposure
BSA retention % after exposure
Table 2.
Biomax 10
Competitor’s
10K
Figure 4. Caustic resistance of
Biomax membrane versus conventional
Polyethersulfone UF membrane
After 100 hours in 600 ppm
chlorine, the Biomax 10 membrane
showed no appreciable change in air
integrity or BSA retention (Table 2).
Post-Run
After
99.9
Retention (%)
After 380 hours in 1 N NaOH at
50 °C, Biomax membranes show
consistently better retention and
integrity than competitive polyethersulfone UF membranes. Caustic cleaning
regimens have no effect on the ability
of the Biomax membrane to retain
proteins and do not adversely affect
integrity (Figure 4).
A rigorous cleaning regimen of
caustic plus hypochlorite restores
normalized water permeability (NWP)
to near initial values following
sequential process runs (Figure 5).
250
100
Superior Chemical Resistance
Results in Excellent Cleanability
Figure 5. Consistent return of water
permeability after cleaning
Sample A
Sample B
7
99.97
3
99.97
8
99.97
10
99.97
Biomax Membrane Dextran Rejection
1
Rejection Coefficient
0.9
PBCC
PBFC
0.8
PBQK
PBGC
PBHK
PBTK
0.7
0.6
0.5
100000
10000
1000
1000000
Molecular Weight
Figure 7. UF membrane Dextran retention profile
Specifications
Membrane
Relative protein binding
Composite polyethersulfone with void-free structure,
caompatible with solutions having a pH between
1 and 14.
Low to moderate, for use with protein solutions
containing more than 0.1 mg/mL of protein.
Biomax Applications
To Place an Order or Receive
Technical Assistance
Lenntech
info@lenntech.com Tel. +31-152-610-900
www.lenntech.com Fax. +31-152-616-289
Biomax
Type
Membrane
Code
NMWL*
(kDa)
Typical
Application
Biomax 5
PBCC
5
Growth factors, hormones
Biomax 8
PBFC
8
Growth factors, hormones
Biomax 10
PBGC
10
Albumin, hemoglobin
Biomax 30
PBTK
30
Enzymes
Biomax 50
PBQK
50
IgGs
Biomax 100
PBHK
100
Small viruses, antigens
Biomax 300
PBMK
300
IgMs, large viruses
Biomax 500
PBVK
500
Large viruses, colloids, particulates
Biomax 1000
PBXK
1000
Large viruses, cells, colloids,
particulates
* Nominal Molecular Weight Limit
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Millipore and Pellicon are registered trademarks of
Millipore Corporation.
Ultracell is a trademark of Millipore Corporation.
Lit. No. PF1402EN00 Rev. A 06/02 02-176
Printed in U.S.A.
© 2002, Millipore Corporation, Bedford, MA 01730
U.S.A. All rights reserved.
Product Usage
Biomax membranes are found in Pellicon® XL devices and Pellicon 2 Cassettes
from Millipore.