Overzicht • • • • • • Lifewatch Lifewatch.eu Lifewatch.be Taxonomic backbone Data archeology Observatory – – – – – – – – Sampling cruises Benthos Bacteria Phytoplankton Zooplankton Fish Birds Mammals • Lifewatch portal and services Lifewatch • Large scale european research infrastructure • Virtual laboratory for study of biodiversity • E-science, web services, data services, ICT Infrastructure, HPC, GRID, BIG data, workflow • Increase data generation, real time monitoring data, biosensors • Construction : 5 years, operation > 20 years Lifewatch.eu Lifewatch.be Lifewatch.be Antarctic observations Lifewatch.be Habitat data from remote sensing Lifewatch.be Data archeology Lifewatch.be Taxonomic backbone SPECIES REGISTERS National LifeWatch Sweden BeRMS FloraBank VIS FaEU RAMS Regional PESI Eu+MedPlantbase ERMS Thematic NeMys Fishbase IF Algaebase IPNI Mollusca Global FADA WoRMS CoL LifeWatch Taxonomic Backbone IMIS WoRMS LITERATURE GBIF AntaBIF OBIS nodes EoL TraitBank OBIS POLYTRAITS EMODnet EurOBIS MedOBIS OBIS Black Sea BIOTIC PANGAEA EU data EU institutes BOLD Aphia DB OBIS Environmental databases GenBANK ECOLOGY (traits) GENETICS SPECIES OBSERVATIONS BHL SPECIES REGISTERS National LifeWatch Sweden BeRMS FloraBank VIS FaEU RAMS Regional PESI Eu+MedPlantbase ERMS Thematic NeMys Fishbase IF Algaebase IPNI MolluscaBase Global FADA WoRMS CoL LifeWatch Taxonomic Backbone IMIS WoRMS LITERATURE GBIF AntaBIF OBIS nodes EoL TraitBank OBIS POLYTRAITS EMODnet EurOBIS MedOBIS OBIS Black Sea BIOTIC PANGAEA EU data EU institutes BOLD Aphia DB OBIS Environmental databases GenBANK ECOLOGY (traits) GENETICS SPECIES OBSERVATIONS BHL Marine observatory Bird tagging + web cams Real-time access And data services for users Data transfert Internet connection Bat acoustic recorder Monthly surveys - sample library - continous sensor data Flow cytometer Video Plankton Recorder Zooscan Sensor platform (Lotto) - CTD - hydrophones - under water camera Fish tagging Multi-beam recordings Sampling cruises • Cruises Underway data: Temperature, Salinity, CO2, fluorometry Depth profile: Temperature, Salinity, Turbidity, Oxygen, PAR, pH Samples: Water samples: nutrients, pigments and suspended matter, Zoöplankton, Fytoplankton, Macrobenthos Bacteria • Ocean Sampling Day – – – – – – 21th June 2014 4 pilots 2012 & 2013 Samples for metagenomic analysis on prokaryotes NG Sequencing by Argonne National Laboratory (US) Bioarchiving at Smithsonian (GGI) OSD Plans • ILVO genomics platform – Bacterial communities on plastic waste • Sampling with volunteers (INBO) Benthos • • • - Van Veen grabs Macrobenthos (> 1mm sieve) Sample library: EMBRC link - bioarchive Samples available for research at request Long term storage Basic processing: molluscs, amphipods, polychaetes, Combination with SPI images & video grab Fytoplankton Flow cytometer • Laser based instrument for particle detection and characterisation in fluids • Counting and characterisation of phytoplankton particles -> taxonomic composition and abundance • Image acquisition: particle scattering + fluorescence • Analysis: clustering software (Easyclus [Matlab], FCM [R], …) Fytoplankton Status: • Training sessions hardware & software • Workshops DYMAPHY (Boulogne, Wimereux) • Tested and tuned in MSO lab • Installed on board of SS – Connected to continuous water flow – Installed side by side with FRRF • PhD study by Marine@Ugent starting October 2014 Zooplankton Video Plankton Recorder (VPR) • Real-time underwater digital camera system + strobe • Rapid quantification of plankton taxonomic composition and abundance • Image acquisition: 30 frames/second of 7.2 ml image volume • Analysis: pattern recognition software (Visual Plankton software [Matlab]) Zooplankton ZooSCAN • Scanning system for digitising zooplankton samples – 2400-4800 dpi; volume 0.2-1 L; – Resistant to salt water, formaldehyde and ethanol – Recognition software that uses comparison with user-developed or imported images • Net sampling -> sample library Zooplankton Status: • VPR build and delivered @MSO • Cable & Winch for deployment VPR under construction by EMCE -> delivery expected June 2014 • Testing planned June-August 2014 • ZooSCAN tested and tuned in MSO -> First results • Further testing and optimizing procedures • PhD study by Marine@Ugent starting October 2014 Fish • Acoustic fish telemetry network • Studying migration, habitat use and behaviour • > 30 acoustic receivers on BPNS & Westerschelde Gadus morhua, Anguilla anguilla, Homarus gammarus, … Fish Status • VLIZ-UGENT-INBO cooperation • Installation: – – – – – • 15 receivers on navigation buoys on BCP 18 + 12 receivers on navigation buoys on WS 1 on tripod MUMM 1 planned for Lotto buoy 4 VR2C cabled receivers planned for windmill farms - Belwind & C-Power - Combination with C-Pod PhD study by Marine@Ugent starting October 2014 A B C D Birds • Tracking of large birds with GPS tags developed by UvA-BiTS • Studying migration and habitat use • 3 ground stations, 11 antennas and 37 GPS loggers deployed 22 for lesser black-backed gulls, 5 for herring gulls, and 10 for the western marsh harrier + web cams on nests Birds Status: • VLIZ-INBO-Ugent cooperation • Zeebrugge: New GPS station in; web cam under negotiation • Oostende: additional anntennas towards city centre • Zwin: base station installed • http://lifewatch.be/birds-historical-data Mammals C-Pods • Harbour Porpoise (& other cetaceans) echolocation clicks at 130 kHz • Fixed sensors: – – – – 1 on Lotto buoy 2 on Belwind windmills 1 in front of Ostend harbour … • Porpoise monitoring cruises - PAM Hydrophone Mammals Bat Corder • Batcorder samples sound at 500 kHz with 16 Bit amplitude resolution • SD card: 64 GB (3-4 month; +/-60.000 calls) • Auto+Timer-Mode • SMS messages (remaining storage capacity, recorded files, battery condition) • Automated Call sequence detection and classification • Locations: – Marine Station Ostend – Observatory De Haan – Thornton Bank C-Power Mammals Bat Corder • First results spring 2014 MSO • Validating results with INBO Pipistrellus pipistrellus • Work on automating data flow and presentation of results Pipistrellus nathusii Marine environmental DNA • Extraction and analysis of genetic material obtained directly from environmental samples • e-DNA (meta)barcoding: PCR with specific primers on short DNA fragments • First successful applications in marine waters (Thomsen et al. 2012) • PhD study by Marine@Ugent starting October 2014 Multibeam echosounder • • • • Acoustic high resolution depth sounding sonar Bathymetry and sediment typology Data generation: water column data 5Tb/year Analysis: data cleaning and validation, chart creation, deriving sediment typology (CARIS, Fledermaus) Bird radar • • • • 3D radar detection of bird movements and density Impact of wind turbines on birds, bird migration research Data generation: 1TB/year (radar images + flight tracks) Analysis: bird detection algorithms, GIS mapping & visualization (CartoDB), migration analysis Weather radar: biological data • Retrieval of biological data from European weather radars • ENRAM - European Network for the Radar surveillance of Animal Movement (COST Action ES1305) • Data generation: 15-23 TB/year • Analysis: bird detection algorithms, GIS mapping & visualization, migration analysis VLIZ projects LifeWatch biodiversity Simon Stevin buoys web cams fish tags data archeology models taxonomic backbone species traits genomics ESFRI / EWI ICOS co2, primary production Simon Stevin Chile cruise ships ESFRI / EWI EMODNET marine data network data portal models data products species traits DG MARE / EWI EMBRC marine biological resources Simon Stevin Mesocosms, aquaria genomics labs ESFRI/ EWI Proj. Zeehond aritifical reefs, platforms buoy web cams fish tags LOTTO VLIZ – C-POWER WIFI WEBCAM BATRECORD Monitoring Buoy Collaborative platform for sensor data processing Sensor network Flowcytometer Processing network Science community eDNA Scripts, flows, algorithms Bird Radar VPR Fish sonar Benthic img 50Tb Sensor data HPC, R, Java Species identifications, field observations, crowd sourcing Remote sensing Hydrophones Bird video Calibration data Bird tracks In situ habitat obs. Fish tags ? Scientific data Archive Big data HPC Not in construction plan? Portal & data services LifeWatch.be data services Access to the LifeWatch sensor networks LifeWatch INBO Building a terrestrial and freshwater observatory Bart Aelterman European Seabirds at Sea Application for fast data entry Unmanned Aerial System High resolution imaging for habitat monitoring Fish tracking network 100+ acoustic tags and receivers Bird radar Automatic bird detection in 5km range Bird tracking network Lightweight, solar powered GPS tags Existing INBO systems e.g. 440.000+ Fish occurrences Publish via IPT data.inbo.be/ipt Register with GBIF Discoverability GBIF webservices CartoDB API We can build our own tools CC0 + norms Allow data to be used Next steps Upscaling of existing network ● Open data policy at INBO ● Continued publication of datasets ● Thanks @LifeWatchINBO lifewatch.inbo.be