Lecture 34: More Analysis – Solitary Confinement Fit Model JMP Output: ANOVA Y: Frequency Construct Model Effects Source Model Error C. Total Solitary Prisoner[Solitary] Day Solitary*Day df Sum of Squares 23 2375.6 36 102.0 59 2477.6 1 JMP Output: Effect Tests Source Solitary Prisoner[Solitary] Day Solitary*Day df Sum of Squares 1 248.0667 18 1610.2000 2 256.9000 2 260.4333 2 Comment JMP calculates all the sums of squares and degrees of freedom correctly, however, we have not told JMP that a split plot design was used to collect the data and so some of the F tests are incorrect. 3 Analysis of Variance Source Solitary Prisoner[Solitary] Day Solitary*Day Error C. Total 4 Effect of Solitary df Sum of Squares Mean Square 1 248.0667 248.0667 18 1610.2000 89.4556 2 256.9000 128.4500 2 260.4333 130.2170 36 102.0000 2.8333 59 2477.6000 5 Because solitary confinement condition (Yes, or No) is assigned to prisoners completely at random, the Prisoner[Solitary] source of variation quantifies the random error variation for evaluating the effect of solitary confinement. 6 1 Lecture 34: More Analysis – Solitary Confinement Effect of Solitary JMP The F statistics used to evaluate the effect of solitary confinement should be the ratio of the mean square for Solitary to the mean square for Prisoner[Solitary]. To get JMP to do this you need to tell JMP that the random error for Solitary is quantified by Prisoner[Solitary]. Highlight Prisoner[Solitary] in the Construct Model Effects box and go to Attributes – Random Effect. 7 8 JMP Effect of Solitary You will need to change the Method from REML (Recommended) to EMS (Traditional) in the upper right corner of the Fit Model dialog box. F = (248.067)/89.4556 = 2.7731, P-value = 0.1132. The P-value is > 0.05, so there is no statistically significant difference between solitary and no solitary. 9 Comment 10 Effect of Day The difference in means, prisoners not in solitary have a mean frequency that is 4 Hz higher than those in solitary, is not statistically significant because the natural variation among prisoners treated the same is so large. F = (128.45)/2.833 = 45.3353, P-value < 0.0001. The P-value is so small, there are differences among some of the day mean frequencies. 11 12 2 Lecture 34: More Analysis – Solitary Confinement Multiple Comparisons ∗ 2.02809 2.833 Multiple Comparisons Day 1 4 7 2 2 20 Mean 16.65 12.95 11.80 A B C Days not connected by the same letter are significantly different. 1.08Hz 13 14 Linear Contrast Linear Contrast The levels for Day are equally spaced numerical values. Is there a linear relationship between Day and average frequency? Day Coeff Mean 1 –1 16.65 4 0 12.95 7 –1 11.80 Contrast = –4.85 SSContrast=20(–4.85)2/2 = 235.225 15 16 Linear Contrast Quadratic Contrast F = 235.225/2.833 = 83.03 P-value < 0.0001 The P-value is small. There is a statistically significant linear relationship between day and average frequency. F = 21.675/2.833 = 7.65 P-value = 0.0089 The P-value is less than 0.05. There is a statistically significant quadratic contrast. 17 18 3 Lecture 34: More Analysis – Solitary Confinement Comment Interaction Effects F = (130.217)/2.833 = 45.9588, P-value < 0.0001. The small P-value indicates that there is a statistically significant interaction between Solitary and Day. As day increases, average frequency decreases. The decrease is faster at first and not as fast as you approach day 7. 19 20 Interaction Effects There is no effect of day when prisoners are not in solitary confinement. For prisoners in solitary confinement, as day increases, average frequency decreases. 21 22 Comment Comment The interaction effect is the most important clue as to what is happening. There is a negative effect on brain wave frequency the longer one stays in solitary confinement. The decrease in average frequency from day 1 to day 4 is greater than that from day 4 to day 7. 23 24 4