Response of wheat and barley varieties to phosphorus fertilizer, 2010 M. J. Ottman Summary Phosphorus fertilizer represents a significant portion of the cost of producing small grains. Some evidence exists that there are differences in the ability of small grain varieties to take phosphorus up from the soil and utilize this nutrient in the grain. The objective of this study is to determine if barley and wheat varieties grown in Arizona differ in their response to phosphorus fertilizer. A study was conducted for the second year at the Maricopa Agricultural Center testing the response of 7 barley and 14 wheat (12 durum wheat and 2 bread wheat) varieties to 2 phosphorus rates (0 and 100 lbs P2O5/acre). The grain yield increase due to phosphorus application averaged across varieties in 2010 was 170 lbs/acre for barley (not statistically significant) and 545 lbs/acre for wheat. The grain yield increase averaged across varieties and years was 331 lbs/acre for barley and 577 lbs/acre for wheat. The barley and wheat varieties did not differ in their grain yield increase due to phosphorus fertilizer in 2010. However, based on 2 years of results, we were able to detect differences among wheat but not barley varieties in their response to P fertilizer. The yield response to P fertilizer (100 lbs P2O5/acre) among durum wheat varieties varied from 331 lbs/acre for Alamo to 1063 lbs/acre for Orita. Yecora rojo, a bread wheat, did not respond to P fertilizer. Introduction Phosphorus fertilizer costs have increased dramatically in the past few years. In small grain production, fertilizer represents a significant proportion of the cost of production. The availability of soil P can be influenced by root exudates, which are under genetic control (Rengel, 2002). Small grain varieties may differ in their response to phosphorus fertilizer due to the presence or absence of these exudates or other factors (Davies et al., 2002). Citric acid is one of the root exudates that have been identified and related to phosphorus availability. The objective of this study is to determine if wheat and barley varieties grown in Arizona differ in their response to phosphorus fertilizer. This is the second year of the study. Procedure A study was conducted at the University of Arizona Maricopa Agricultural Center to determine if wheat and barley varieties respond to phosphorus fertilizer differently. The soil type was a Casa Grande sandy loam with a preplant soil phosphate level of 2.0 ppm P. P fertilizer treatments were applied before planting at rates of 0 and 100 lbs P2O5/acre using triple super phosphate (0-45-0) as a fertilizer source. The P fertilizer was applied by hand to plots 5 ft x 20 ft in size. The seed was planted with a cone planter in seven rows spaced 7 inches apart and 20 ft long. The seeding rate was approximately 100 lbs/acre for durum varieties and 85 lbs/acre for barley varieties. The experimental design of the P x Variety Study was a split plot with varieties (7 barley and 14 wheat [12 durum wheat and 2 bread wheats]) as main plots and P rate (0 and 100 lbs P2O5/acre) as subplots, and replicated 6 times. A P Rate Study with a wider range of P rates (0, 25, 50, 75, 100, 150 and 200 lbs P2O5/acre) and a single variety (Gustoe barley and Kronos durum) was also conducted to determine if the P rate of 100 lbs P2O5/acre is adequate for optimum yield. Cultural practices are listed in Table 1. The following data was collected: grain yield, test weight, plant height, lodging, heading, flowering, physiological maturity, grain P, grain protein, HVAC, and light interception on Feb 12 at the 5 leaf stage. Grain was harvested with a small plot combine and yields are expressed on an “as is” moisture basis. HVAC was determined from 10 g of seed. Grain protein was calculated using the combustion method to obtain total N, which was multiplied by 6.25 (barley) or 5.7 (wheat) to obtain protein content and expressed on a 12% moisture basis. Flowering is defined as when about half of the heads are shedding pollen and physiological maturity is defined as when the glumes turn brown. Light interception was determined by 2010 Forage & Grain Report, College of Agriculture and Life Sciences, University of Arizona 56 dividing the average of six readings from a sunfleck ceptometer at ground level by incident light level. Abbreviations for the sources of varieties are: APB = Arizona Plant Breeders, WPB = Western Plant Breeders, WWW = World Wide Wheat, UC = University of California. Results and Discussion This growing season was characterized by below average temperature and above average rainfall (Table 2). Temperatures were especially cool during the months of March, April, and May. The month of January was especially wet. Due to the cool weather, the growing season was extended by 1-2 weeks compared to normal. P rate study (6 P rates and 1 barley and 1 wheat variety): Grain yields of Gustoe barley were not affected by phosphorus rates from 0 to 200 lbs P2O5/acre, whereas grain yield of Kronos durum increased linearly with P rate (Table 3). P rate also decreased time to heading; increased grain P content; and increased light interception measured on Feb 12. P x variety study (2 P rates and 7 barley and 14 wheat varieties) We measured a P rate response to many variables (Tables 4-6). P rate increased yield (wheat), test weight (wheat), plant height (barley), decreased time to heading and maturity, increased light interception as measured on Feb 12, and increased grain phosphorus concentration. The varieties responded to an application of 100 lbs P2O5/acre in a similar manner for all of the variables measured (Tables 4-6). The results from 2010 do not provide any evidence that barley and wheat varieties respond differently to P fertilizer. However, if the results from 2009 and 2010 are combined, we were able to detect differences among wheat but not barley varieties in their response to P fertilizer. The yield response to P fertilizer (100 lbs P 2O5/acre) among durum wheat varieties varied from 331 lbs/acre for Alamo to 1063 lbs/acre for Orita. Yecora rojo, a bread wheat, did not respond to P fertilizer. Acknowledgments Financial support for this project was received from the Arizona Grain Research and Promotion Council and the Arizona Crop Improvement Association. The technical assistance of Mary Comeau and Mike Sheedy is greatly appreciated. References Davies, T.G.E., J. Ying, Q. Xu, Z.S. Li, J. Li, and R. Gordon-Weeks. 2002. Expression analysis of putative highaffinity phosphate transporters in Chinese winter wheats. Plant, Cell and Environment. 25:1325-1339. Rengel, Z. 2002. Genetic control of root exudation. Plant and Soil 245:59-70. 2010 Forage & Grain Report, College of Agriculture and Life Sciences, University of Arizona 57 Table 1. Cultural practices for the small grain phosphorus trial at Maricopa. Cultural information Maricopa Previous crop Fallow Soil texture Sandy clay loam Planting date 12/10/09 Irrigations 6 12/10, 12/21, 2/17, 3/17, 4/1, 4/15, 5/1 Nitrogen (lbs N/a) 220 12/12: 51 (46-0-0) 1/28: 24 (32-0-0) 2/25: 76 (32-0-0) 3/13: 74 (32-0-0) Phosphorus (lbs P2O5/acre) 100 Pesticides None Harvest date 6/1 (Durum) 6/3 (Barley) Table 2. Climatic data from AZMET for Maricopa during the 2010 growing season compared to the long-term average. Climate variable Unit Year(s) Dec Jan Feb Mar Apr May Dec-May Max Temp. ºF ºF 2010 Avg 64 65 66 66 68 70 73 77 82 85 90 95 74 76 Min Temp. ºF ºF 2010 Avg 33 35 38 36 41 39 43 44 49 51 55 60 43 44 Ppt. inches inches 2010 Avg 0.39 0.62 2.64 0.72 0.56 0.85 0.62 0.79 0.00 0.26 0.00 0.20 4.21 3.43 2010 Forage & Grain Report, College of Agriculture and Life Sciences, University of Arizona 58 Table 3. Phosphorus rate effect on grain yield and other plant characteristics for ‘Gustoe’ barley and ‘Kronos’ durum. Grain Phosphorus % Grain Protein % HVAC % Light interception (Feb 12) % P rate lbs/A Yield lbs/A Test Weight lbs/bu Plant Height inches 0 25 50 75 100 150 200 Avg 5704 6438 6552 6130 5653 5623 5731 5976 50.3 50.2 49.5 50.1 49.9 50.3 50.5 50.1 34.3 33.5 33.8 34.3 35.3 33.5 33.8 34.0 4/04 4/01 3/30 3/31 3/31 3/31 3/31 3/31 Barley ----------------- 5/15 5/14 5/11 5/13 5/12 5/12 5/12 5/13 0.219 0.224 0.230 0.245 0.247 0.258 0.280 0.243 11.6 11.9 11.8 11.6 11.7 11.4 11.7 11.7 ----------------- 61 84 81 90 86 87 84 82 P rate Linear Quad Cubic NS NS NS NS NS NS + NS NS NS NS NS ** ** ** * --------- NS NS NS NS ** ** NS NS NS NS NS * --------- ** ** ** + 0 25 50 75 100 150 200 Avg 6913 7353 7519 7474 7895 7186 8246 7512 62.0 62.3 62.3 62.5 62.6 62.5 62.5 62.4 36.0 36.5 36.5 35.5 36.3 35.8 35.3 36.0 3/24 3/22 3/22 3/22 3/22 3/22 3/22 3/22 Durum . 3/26 3/26 3/26 3/26 3/26 3/26 3/26 5/15 5/15 5/15 5/14 5/13 5/14 5/15 5/14 0.244 0.250 0.251 0.276 0.263 0.273 0.298 0.265 14.5 14.5 14.5 14.8 14.7 14.6 14.7 14.6 100 100 100 100 100 100 100 100 49 68 68 73 75 76 72 69 P rate Linear Quad Cubic * * NS * NS NS NS NS NS NS NS NS ** ** ** ** NS NS NS NS NS NS NS NS ** ** NS NS NS NS NS NS NS NS NS + ** ** ** NS Heading Flowering Maturity 2010 Forage & Grain Report, College of Agriculture and Life Sciences, University of Arizona 59 Table 4. Grain yield, test weight, and plant height of barley and wheat varieties as affected by phosphorus fertilizer rates of 0 and 100 lbs P2O5/acre. “Response” refers to the difference between the phosphorus rates. The wheat varieties are durums except for Yecora Rojo and Joaquin, which are bread wheats. Grain Yield Entry Source Chico Cochise Gustoe Nebula Commander Max Baretta Avg WPB WPB WPB WPB WWW WWW APB --- LSD.05* Entry P rate Entry x P Alamo Havasu Orita WPB-881 Crown Duraking Q-Max Kronos Sky Ocotillo Westmore Maestrale Yecora Rojo Joaquin Avg LSD.05* Entry P rate Entry x P WPB WPB WPB WPB WWW WWW WWW APB APB APB APB Allstar Public WPB --- Test Weight Plant Height Phosphorus 0 lb/A 100 lb/A Response 0 lb/A 100 lb/A Response 0 lb/A 100 lb/A Response lbs/A lbs/bu inches 5802 4906 6798 6492 6662 6035 7200 6258 5840 5127 6739 6929 7160 5992 6989 6397 39 220 268 438 498 -43 -211 170 49.7 50.7 50.1 51.0 49.6 49.8 50.4 50.2 781 ** NS NS 781 NS 0.7 ** NS + 6573 7166 6567 6797 5558 6619 4708 6660 7510 6402 7143 6004 7100 7367 6581 6492 7989 7693 7123 6351 7389 5399 7442 8028 6922 7479 6711 6824 8106 7139 -81 823 999 355 793 770 692 782 518 520 336 707 -276 739 545 64.1 63.8 60.2 62.0 57.6 62.2 58.3 61.7 61.2 62.3 62.7 63.3 63.9 63.5 61.9 735 ** ** NS 735 NS 0.9 ** ** NS Barley 50.3 51.2 49.9 50.8 49.6 49.4 51.2 50.3 0.6 0.6 -0.2 -0.2 0.0 -0.4 0.8 0.2 31.7 34.8 34.0 37.0 33.8 34.0 34.2 34.2 31.8 36.0 34.5 37.0 35.2 34.8 35.2 34.9 0.2 1.2 0.5 0.0 1.3 0.8 1.0 0.7 0.7 0.8 1.4 ** ** NS 1.4 NS Wheat 64.1 64.5 61.3 62.1 59.1 62.2 59.2 61.9 61.6 62.4 63.2 63.5 63.4 63.5 62.3 0.0 0.7 1.1 0.1 1.5 0.0 0.9 0.2 0.3 0.1 0.5 0.2 -0.6 0.0 0.4 36.5 36.2 36.0 37.2 37.8 35.7 37.8 36.7 34.7 37.8 36.0 37.5 33.3 36.7 36.4 36.5 37.0 36.5 36.7 38.0 36.3 37.7 36.2 34.0 38.3 36.3 37.7 34.7 36.8 36.6 0.0 0.8 0.5 -0.5 0.2 0.7 -0.2 -0.5 -0.7 0.5 0.3 0.2 1.3 0.2 0.2 0.9 NS 1.1 ** NS NS 1.1 NS * LSD.05 = least significant difference between means within a column with a 5% or less probability the difference is due to chance. 2010 Forage & Grain Report, College of Agriculture and Life Sciences, University of Arizona 60 Table 5. Heading, physiological maturity, and light interception of barley and wheat varieties as affected by phosphorus fertilizer rates of 0 and 100 lbs P 2O5/acre. “Response” refers to the difference between the phosphorus rates. The wheat varieties are durums except for Yecora Rojo and Joaquin, which are bread wheats. Heading Entry Source Chico Cochise Gustoe Nebula Commander Max Baretta Avg WPB WPB WPB WPB WWW WWW APB --- LSD.05* Entry P rate Entry x P Alamo Havasu Orita WPB-881 Crown Duraking Q-Max Kronos Sky Ocotillo Westmore Maestrale Yecora Rojo Joaquin Avg LSD.05* Entry P rate Entry x P WPB WPB WPB WPB WWW WWW WWW APB APB APB APB Allstar Public WPB --- Physiological Maturity Light Interception (Feb 12) Phosphorus 0 lb/A 100 lb/A Response 0 lb/A 100 lb/A Response 0 lb/A 100 lb/A Response % of incident 3/28 3/15 3/31 3/27 3/30 4/01 3/27 3/27 3/25 3/15 3/29 3/23 3/28 3/28 3/25 3/25 -3 0 -2 -4 -2 -2 -3 -2 5/14 5/03 5/14 5/13 5/13 5/17 5/11 5/12 2 ** ** NS 2 2 4 ** ** NS 3/25 3/24 3/29 3/26 3/30 3/30 4/03 3/25 3/25 3/25 3/24 3/27 3/23 3/22 3/26 3/22 3/22 3/27 3/22 3/27 3/25 3/28 3/22 3/22 3/23 3/22 3/24 3/22 3/21 3/23 -3 -3 -3 -4 -3 -4 -6 -3 -3 -3 -2 -3 -2 -2 -3 5/18 5/15 5/17 5/15 5/19 5/17 5/21 5/15 5/18 5/14 5/14 5/14 5/14 5/16 5/16 1 ** ** NS 1 NS 2 ** ** ** Barley 5/11 5/02 5/12 5/06 5/13 5/13 5/07 5/09 -4 -1 -2 -7 1 -3 -4 -3 48 60 72 56 60 62 58 59 64 80 80 82 79 77 77 77 17 20 8 26 19 15 19 18 4 NS 15 * ** NS 15 NS Wheat 5/18 5/14 5/16 5/15 5/16 5/17 5/18 5/15 5/17 5/15 5/13 5/13 5/08 5/15 5/15 0 0 -1 0 -3 -1 -3 0 -1 1 -1 0 -5 -1 -1 62 44 49 68 41 33 25 44 51 53 58 51 56 60 50 62 69 69 70 52 45 26 65 72 67 65 66 72 58 61 1 25 20 2 11 12 0 21 22 14 7 15 16 -1 12 2 3 17 ** ** NS 17 18 * LSD.05 = least significant difference between means within a column with a 5% or less probability the difference is due to chance. 2010 Forage & Grain Report, College of Agriculture and Life Sciences, University of Arizona 61 Table 6. Grain phosphorus, grain protein, and percentage of kernels “hard and vitreous and of amber color” (HVAC) of barley and wheat varieties as affected by phosphorus fertilizer rates of 0 and 100 lbs P 2O5/acre. “Response” refers to the difference between the phosphorus rates. The wheat varieties are durums except for Yecora Rojo and Joaquin, which are bread wheats. Grain Phosphorus Entry Source Chico Cochise Gustoe Nebula Commander Max Baretta Avg WPB WPB WPB WPB WWW WWW APB --- LSD.05* Entry P rate Entry x P Alamo Havasu Orita WPB-881 Crown Duraking Q-Max Kronos Sky Ocotillo Westmore Maestrale Yecora Rojo Joaquin Avg LSD.05* Entry P rate Entry x P WPB WPB WPB WPB WWW WWW WWW APB APB APB APB Allstar Public WPB --- Grain Protein Phosphorus 0 lb/A 100 lb/A Response 0 lb/A 100 lb/A Response 0 lb/A % % 0.24 0.26 0.22 0.25 0.24 0.22 0.25 0.24 0.27 0.28 0.25 0.26 0.26 0.27 0.27 0.27 0.03 0.02 0.03 0.02 0.02 0.05 0.02 0.03 12.2 11.8 11.9 12.4 12.4 12.8 13.2 12.4 NS NS ** NS NS NS 0.8 * + NS 0.30 0.27 0.29 0.27 0.29 0.28 0.28 0.28 0.27 0.30 0.27 0.27 0.28 0.27 0.28 0.30 0.31 0.30 0.31 0.31 0.29 0.30 0.30 0.30 0.31 0.30 0.29 0.30 0.30 0.30 0.01 0.04 0.01 0.04 0.02 0.01 0.02 0.02 0.03 0.01 0.03 0.02 0.03 0.02 0.02 15.1 14.8 15.7 14.5 15.6 14.7 15.7 14.8 14.0 15.0 15.8 14.6 14.7 14.6 15.0 NS NS ** NS NS NS 0.7 ** NS NS HVAC 100 lb/A Response % Barley 11.9 11.4 12.1 12.2 12.3 12.7 12.2 12.1 -0.3 -0.4 0.1 -0.2 -0.1 -0.1 -1.0 -0.3 ----------------- ----------------- ----------------- 0.8 NS --------- --- --- Wheat 15.1 14.6 15.6 14.5 15.4 14.6 14.9 15.0 14.0 14.9 16.6 14.3 15.1 14.9 15.0 0.0 -0.2 -0.1 0.0 -0.2 -0.1 -0.8 0.2 0.0 -0.1 0.9 -0.3 0.4 0.4 0.0 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.7 NS --* NS NS --- NS * LSD.05 = least significant difference between means within a column with a 5% or less probability the difference is due to chance. 2010 Forage & Grain Report, College of Agriculture and Life Sciences, University of Arizona 62