Results of New Cultivar Selection Trials for Lemon in Arizona –2004-051 Glenn C. Wright Department of Plant Sciences, U. of A., Yuma Mesa Agriculture Center, Yuma, AZ Abstract Three lemon cultivar selection trials are being conducted at the Yuma Mesa Agriculture Center in Somerton, AZ. Data from these trials suggest that ‘ Li mone r oFi no49’selections may be a suitable alternative for the varieties most commonly planted in Southwest Arizona today. ‘ Fe mmi ne l l o’ and ‘ Vi l l af r anc a’mi ghtal s obepl ant e d on an experimental basis Introduction The Arizona lemon industry has historically relied on a small number of lemon cultivar selections. Int h e1950’ s , the i n du s t r ywa se s t a bl i s h e d wi t h‘ De s e r tLi s bon ’ , however within a few years, ‘ De s e r tLi s bon ’wa seclipsed in popu l a r i t yby‘ Fr os tNu c e l l a rLi s bon ’t h eon l yn u c e l l a rc l on a ls e l e c t i onoft h e‘ Li s bon ’cultivar. Other minor s e l e c t i on sof‘ Li s bon ’t h a twere planted in Arizona f r om t h e1960’ st h r ou g ht h e1980’ sincluded ‘ Mon r oe ’ ,‘ Pr i or ’ , a n d‘ Ros e n be r g e r ’ . Be g i n n i n gi nt h el a t e1980’ s , n e w pl a n t i n g swe r ee s t a bl i s h e du s i n g‘ Li mon e i r a8A Lisbon’ . Mor er e c e n t l y ,‘ Cor on aFoot h i l l sLi s bon ’i si n c r e a s i n g l ypopu l a r .‘ Al l e nEu r e k a ’h a sa l s obe e noc c a s i on a l l ypl a n t e d in Arizona. All of these represent clonal selections of outstanding trees that were then propagated. Typically, they are identified by their originator or place of origin, and are valuable to Arizona growers because of their high vigor, high productivity, precocity (trees bear at an early age), earliness (a high percentage of the fruit can be harvested before 1 November), short thorns and good fruit quality. When a commonly grown lemon cultivar selection is gradually replaced in the industry, the new selection typically is improved in one of these characteristics. Sometimes a cultivar selection may be replaced because of a n e g a t i v ec h a r a c t e r i s t i c . Su c hwa st h ec a s ewi t h‘ Fr os tNu c e l l a rLi s bon ’ which appears to be susceptible to brown heartwood rot. From t h el a t e1980’ s ,t ot h ee a r l y1990’ s ,Arizona lemon growers have received their information about new cultivar selections through word of mouth or from nursery sources, since there were no trials planted in the state. With this in mind, we have planted three new lemon cultivar selection trials in 1995, 1997 and 1998, all located at the Yuma Mesa Agricultural Center. The 1997 and 1998 trial results are presented here. 1 1 The author wishes to thank Mr. Phillip Tilt, Mr. Marco Peña, Mr. Arturo Moreno, and the Yuma Mesa Fruit Gr owe r ’ sAs s oc i a t i onf ort h e i ra s s i s t a n c ei nc ompl e t i n gt h i spr oj e c t .Th ea u t h orwou l da l s ol i k et ot h a n kt h eAr i z on a Citrus Research Council for supporting this research. This is a partial final report for project 2004-08 –Citrus rootstock and cultivar breeding and evaluation for the Arizona citrus industry –2004-05. Materials and Methods 1997 Lemon Cultivar Selection Trial. This trial, comprising 13 cultivar selections, was established in March 1997 in Block 22 of the Yuma Mesa Agricultural Center, near Yuma, Arizona. The land was laser leveled and fumigated prior to planting. Trees were planted on an 8-m x 8-m spacing. Fifteen trees of each selection were planted. This trial includes the following selections: ‘ Al l e nEu r e k a ’–Th emos tc ommona n dpopu l a r‘ Eu r e k a ’s e l e c t i onpl a n t e di nAr i z on a .Or i g i n a t e di nSa n t a Paula, CA ‘ Ar a n c i n o’–A minor Italian cultivar, with rounded fruit, a short nipple and thick rind. Fruit is seedy. ‘ Be r n a ’( ‘ Ve r n a ’ )–The common summer lemon of Spain. Thornless tree produces medium to large fruit with few seeds. ‘ Ca v e r sLi s bon ’–Av i g or ou s‘ Li s bon ’s e l e c t i onor i g i n a t i n gi nUpl a n d,CA. ‘ Ca s c a deEu r e k a ’–Another, less-commonly planted, vigorous selection that originated in San Diego County, CA. ‘ CookEu r e k a ’–A selection from Limoneira Del Mar Ranch, Ventura County, California. ‘ Cor pa c i ’–A minor Italian cultivar from Sicily. Vigorous, thorny trees are reportedly productive. Fruit matures early and has few seeds. ‘ Fe mmi n e l l oComu n e ’–Italian, everbearing cultivar. ‘ Li mon e i r a8ALi s bon ’–A vigorous selection originating from the Limoneira Ranch, Ventura County, CA. The most popular lemon planted in Arizona today. ‘ Li mon e r oFi n o49’–The chief winter lemon of Spain. Reportedly vigorous, thorny and highly productive. Early producer with uniform yield. Fruit is spherical to oval, with a smooth rind and a relatively short nipple. Relative high acid and about five seeds per fruit. ‘ Pr i mof i or i ’–Or i g i n a t e di nSpa i n .Si mi l a rt ot h e‘ Li mon e r oFi n o49’de s c r i be da bov e . ‘ Sa n t aTe r e s a ’(Femminello Santa Teresa) –Si mi l a rt o‘ Fe mmi n e l l oComu n e ’ ,bu tr e s i s t a n tt ot h eMa l Secco disease prevalent in Italy. ‘ Vi l l a f r a n c a ’–Said to be of Sicilian origin, introduced into Florida in 1875. Formerly planted in Ca l i f or n i a ,bu tofl i t t l ei mpor t a n c et h e r et oda y . Fr u i ta n dt r e ec h a r a c t e r i s t i c ss i mi l a rt o‘ Eu r e k a ’ ,bu t produces mainly a winter crop. 1998 Lemon Cultivar Selection Trial. This trial, comprising 7 cultivar selections, was established in late September 1998 in Block 14 of the Yuma Mesa Agricultural Center, near Yuma, Arizona. The land was laser leveled and fumigated prior to planting. Trees were planted on an 8-m x 8-m spacing. Fifteen trees of each selection were pl a n t e d.Th i st r i a li n c l u de st h e‘ Li mon e i r a8ALi s bon ’de s c r i be da bov ea swe l la st h ef ol l owi n ga ddi t i on a lcultivar selections: ‘ Dr .St r on gLi s bon ’–Originated at the Glen Good ranch, Santa Paula, CA. Large fruit, but tree is reportedly precocious. ‘ Ge n oa ’–Si mi l a rt ot h e‘ Vi l l a f r a n c a ’ ,i mpor t e df r omI t a l yt ot h eU. S.i n1881. ‘ La pi t h ot i k i ’–Originated in Cyprus. Reportedly harvested from September until March. Fruit is tapered at both ends. ‘ Mon r oeLi s bon ’–Vigorous selection. Reportedly bears early, but fruit is small and coarse. ‘ Ta y l orEu r e k a ’–A nucellar selection, originating in Australia. Reportedly produces late. ‘ Wa l k e rLi s bon ’–Vigorous selection from California. Yield data is collected during the fall and winter. Trees were ring or strip-picked as noted below. For 2004-05, trees in the 1997 trial were strip picked on 12-7-04. Trees in the 1998 trial were ring picked on 10-7-04 and strip picked on 1-19-05 For each harvest date, the entire quantity of harvested fruit from each tree was passed through an automated electronic eye sorter (Autoline, Inc., Reedley, CA), which provides weight, color, exterior quality and size data for each fruit. Fruit packout data is reported on a percentage basis. Fruit quality data, including brix, peel thickness, percentage juice, pH, and the total soluble solids to total acid ratio was collected for the 1998 trial. All data was analyzed using SPSS 11.0 for Windows (SPSS Inc., Chicago, Illinois). Results and Discussion 1997 Lemon Selection Trial. Yields of this trial, since its inception, are found in Figure 1. Because of the large number of selections in this trial, the graph has been split for ease of viewing. Selections that have had superior yields since the beginning, i n c l u de‘ Ca s c a deEu r e k a ’ ,‘ Cook ’Eu r e k a ,‘ Li mon e i r a8ALi s bon ’ ,‘ Li mon e r oFi n o49’ , ‘ Pr i mof i or i ’ ,‘ Fe mmi n e l l oComu n e ’a n d‘ Vi l l a f r a n c a ’ . Yields of most of the selections declined in 2004-05 due to flower and fruitlet loss during untimely heat in the Spring and the influence of a large crop in 2003-04. All the Eu r e k as e l e c t i on sf e l lmor et h a n50%,i n c l u di n g‘ Al l e n ’ ,‘ Ca s c a de ’a n d‘ Cook ’ .‘ Vi l l a f r a n c a ’ ,‘ Fe mmi n e l l oComu n e ’ a n d‘ Li mon e i r a8A’h a ds l i g h tde c r e a s e si ny i e l d,wh i l e‘ Li mon e r oFi n o49’wa st h eon ly selection that had an increased yield versus 2003-04. Yield for the 2004-05 season is shown in Figure 2. ‘ Li mon e r oFi n o49’h a dt h eg r e a t e s ty i e l d,a n d‘ Fe mmi n e l l o Comu n e ’ ,‘ Vi l l a f r a n c a ’a n d‘ Li mon e i r a8A’we r el owe r ,bu ts t i l ls t a t i s t i c a l l yt h es a me .‘ Pr i mof i or i ’a n d‘ Sa n t a Te r e s a ’pe r f or me dt h epoor e s toft h os es e l e c t i on st h a tt y pi c a l l yh a v et h eg r e a t e s ty i e l d.Most of the others performed poorly, with yields of less than 150 lbs. per tree. Packout for the 12-7-04 harvest, of seven of the most promising selections is shown in Figure 3. Fruit size was quite large, a result of the unusually late harvest date. Wh i l emos toft h e s eh a ds i mi l a rs i z e ,‘ Ca v e r s ’ ,‘ Primofiori’ ,a n d ‘ Li mon e r oFi n o49’h a ds l i g h t l yl a r g e rf r u i t ,wh i l e‘ Sa n t aTe r e s a ’a n d‘ Limoneira 8A’h a ds ma l l e rf r u i t .‘ Si n c et h e yields of‘ Ca v e r s ’and Primofiori are so low, it is doubtful that the larger fruit for this cultivar is of any real a dv a n t a g e .‘ Li mon e r oFi n o49h a sh a dl a r g e rf r u i tf ors e v e r a loft h epa s tf e ws e a s on s . Selected fruit quality parameters from the cultivars are shown in Table 1. While there were many similarities be t we e nt h es e l e c t i on s ,af e ws t a n dou t .‘ Sa n t aTe r e s a ’wa sc h a r a c t e r i z e dbyal ow j u i c epH a n dat h i npe e l . ‘ Li mon e r oFi n o49h a dahigh level of acids and a thin peel. 1998 Lemon Cultivar Selection Trial. 2001-02 to 2004-05 yields from this trial are found in Figure 4. Although the ‘ Li mon e i r a8A’i ss t i l lt h ec l a s soft h i strial; 2004-05 is the first year that any other selection has surpassed it in total yield since 2001-02. ‘ Mon r oe ’ ,‘ Wa l k e r ’a n d‘ Dr .St r on g ’Li s bonh a dy i e l dst h a te qu a l e dors u r pa s s e dt h ey i e l dof ‘ Li mon e i r a8A Li s bon ’( Fi g u r e5) .Me a n wh i l e ,‘ Ge n oa ’ ,‘ Ta y l orEu r e k a ’a n d‘ La pi t h i ot i k i ’h a dy i e l ds40t o70% less than the others. All the higher yielding selections had similar quantities of fruit picked in the first harvest, while the three selections that are lagging had less fruit picked early. Fruit size for the 10-17-04 harvest of t h i st r i a li ss h owni nFi g u r e6.Th e‘ La pi t h i ot i k i ’h a sou t s t a n di n gs i z e ,bu ti ti s an elongated lemon, and is probably unsuitable for the US market. Some of this size may be due to its low yield. Th es i z eoft h e‘ Ge n oa ’f r u i tma ya l s obedu et oi t sl owy i e l d.Oft h eot h e r s ,‘ Dr .St r on ga ppe a r sto have the best s i z ea tt h i st i me ,wh i l e‘ Ta y l or ’Eu r e k ah a st h es ma l l e s tf r u i t .For the second harvest, Lapithiotiki again has the largest fruit (Figure 7), while the others are similar. Conclusions One new lemon cultivar selection appears promising for Arizona. This is ‘ Li mon e r oFi n o49’l e mon , because its y i e l dsa r es i mi l a rt ot h a tof‘ Li mon e i r a8A’a n di th a sl a r g e rf r u i ts i z e .‘ Fe mmi n e l l oComu n e ’a n d‘ Vi l l a f r a n c a ’ma y also be suitable, but neither consistently equals or surpasses ‘ Li mon e i r a8ALi s bon ’l e moni nt e r msofoverall yield a n d/ ore a r l i n e s sa sof t e na sdoe s‘ Li mon e r oFi n o49’ .‘ Sa n t aTe r e s a ’had excellent yield in 2002-03, but stumbled for the last two years, and is hobbled by its lateness. In the 1998 trial, no selection has proved to be better than ‘ Li mon e i r a8A’ ,bu t‘ Wa l k e r ’ ,‘ Mon r oe ’a n d‘ Dr .St r on g ’ma ybec on t e n de r s .No other new selections stand out at this time; however this may change as more data is collected. ‘ La pi t h i ot i k i ’h a sl a r g ef r u i t ,bu tl owy i e l ds .‘ Eu r e k a ’ lemons have typically performed poor l yi nc ompa r i s ont ot h e‘ Li s bon s ’ ,a n ds h ou l dn otbec on s i de r e da sa replacement for any of the high-y i e l di n g‘ Li s bon ’s e l e c t i on s . 500 630 Allen Eureka Arancino Berna Cacade Eureka Cavers Lisbon Cook Eureka Corpaci 400 350 504 441 378 250 315 200 252 150 189 100 126 50 63 0 0 500 630 450 567 Femminello Comune Limoneira 8A Lisbon Limonero Fino 49 Primofiori Sta. Teresa Villafranca 400 350 300 504 441 378 150 189 100 126 50 63 0 0 20 0 20 04 - 20 0 20 03 - 20 0 20 02 - 20 0 20 01 - 20 0 00 20 5 252 4 200 3 315 1 250 2 Annual Yield (lbs. per tree) 300 567 Figure 1. 2000-2005 yield of thirteen lemon selections budded to C. macrophylla rootstock. Approximate yield in field boxes per acre (based on 24 x 24 ft. spacing) 450 630 500 450 504 400 AB 350 Yield (lbs. per tree) ABC ABC 378 300 250 BCD 200 BCD 252 BCD CD 150 CD D D 100 126 D D Approximate yield in field boxes per acre (based on 24 x 24 ft. spacing) A 50 0 a nc fr a Vi lla re sa ri Te of Sa nt a im no Pr Fi io 49 on sb o er m Li Li m on ei on ra Fe 8A m m Li in el lo ci pa ka or C re on C oo k Li sb re er s C av de ca as Eu ka a Eu Be rn no ci an Ar C Al le n Eu re ka 0 Selection December 7, 2004 Harvest Figure 2. 2004-05 Yield of thirteen lemon selections budded to C. macrophylla rootstock, separated by harvest time. Bars are significantly different if the lowercase letters above them are different. Cultivar Cavers Lisbon a b Femminello ab Limonero Fino 49 Primofiori Santa Teresa ab c ab ab bc ab 10 abc a abc ab bcde 0 20 30 75 95 115 140 165 200 a bcde Limoneira 8A Lisbon abc abc e Villafranca c b 40 50 60 70 80 90 100 Packout (%) Figure 3. Packout of 13 lemon selections on C. macrophylla rootstock for the 12-7-04 harvest. Bars of the same shade are significantly different if the lowercase letters within them are different. Bars of different shades cannot be compared. Table 1. Selected interior fruit quality parameters for 13 lemon selections on C. macrophylla rootstock. Total Acids Peel thickness Juice pH TSS:TA Selection (%) (mm) Allen Eureka 2.83 abcz 4.74 bcd 1.58 ab 3.92 Arancino 2.85 abc 4.73 cd 1.56 ab 5.24 Berna 2.82 abc 4.90 abcd 1.60 a 4.69 Cascade Eureka 2.93 ab 4.82 abcd 1.57 ab 4.62 Cavers Lisbon 2.77 abc 5.05 abcd 1.44 ab 5.00 Cook Eureka 2.71 bc 4.60 d 1.60 a 4.04 Corpaci 2.85 abc 5.07 abcd 1.50 ab 4.08 Femminello 2.75 abc 5.26 ab 1.46 ab 4.48 Limoneira 8A Lisbon 2.73 bc 5.23 abc 1.41 b 4.00 Limonero Fino 49 2.80 abc 5.27 a 1.44 ab 3.61 Primofiori 2.86 abc 4.90 abcd 1.56 ab 5.02 Santa Teresa 2.67 c 5.09 abcd 1.48 ab 3.64 Villafranca 2.97 a 4.79 abcd 1.58 ab 4.51 z Me a n ss e pa r a t i oni nc ol u mn sbyDu n c a n ’ sMu l t i pl eRa n g eTe s t ,5% l e v e l . 300 200 315 252 150 189 100 126 50 63 0 2001-2002 2002-2003 2003-2004 2004-2005 Harvest Year Figure 4. 2001-02 through 2004-05 yield of seven lemon selections budded to C. macrophylla rootstock. Approximate Yield in Field Boxes per acre (based on 24 x 24 ft. spacing) Yield (lbs. per tree) 250 378 Taylor Eureka Monroe Lisbon Dr. Strong Lisbon Genoa Lapithiotiki Walker Lisbon Limoneira 8A Lisbon Block 14 Lemons 2004-05 Yield 300 October 7, 2004 Harvest January 19, 2005 Harvest Yield(lbs. per tree) 250 200 a a ab a a 150 100 a a 50 bc ab b b a a c c i ik pi th ot La ur e ka e) Selection Figure 5. 2004-05 yield of seven lemon selections budded to C. macrophylla rootstock. Ta yl or E oa en G M on ro e (O ld Li s Li n bo n n sb o Li tr o ng r. S D Li m on ei ra W al 8A ke rL Li is b sb on on 0 B Walker C Limoneira 8A C Selection Dr. Strong A A A AB A A B B B A Monroe BC B B B BC A ABC A 75 Genoa B Taylor AB B C Lapithotiki 10 20 30 40 50 B A A A A 0 B C A C D 60 70 95 115 140 165 200 235 285 80 90 C 100 Packout (%) Figure 6. Packout of seven lemon selections on C. macrophylla rootstock for the 10-7-04 harvest. Bars of the same shade are significantly different if the lowercase letters within them are different. Bars of different shades cannot be compared. Walker C Limoneira 8A C A A Selection Dr. Strong A A A B B Monroe ABC C CD A C A AB 75 Genoa B Taylor D B A C Lapithotiki BCD A 0 10 20 30 BC AB E D C 40 50 60 70 80 95 115 140 165 200 235 285 90 100 Packout (%) Figure 7. Packout of seven lemon selections on C. macrophylla rootstock for the 1-19-05 harvest. Bars of the same shade are significantly different if the lowercase letters within them are different. Bars of different shades cannot be compared.