FBDSGDUPEN
GeneDisc® Ultra-Purifier
Easy, Rapid and Highly-Efficient Instrument for
DNA Purification
Features
Benefits
Computer-assisted purification protocol
Reduce hands-on time
Process up to 48 DNA samples
simultaneously
Flexible throughput fits testing needs
Part of a complete ‘sample to result’
analytical tool
Increase test range progressively
Flexible device setting
Increase user comfort while running
Minimal footprint
Easily integrate and relocate device
Description
Operation Sequence
The GeneDisc system is a complete, rapid, reliable and easyto-use solution for microbial testing in three simple steps from
sample to result. After samples are extracted with the GeneDisc
Ultra-Lyser, the DNA may be purified by utilizing the GeneDisc
Ultra-Purifier. Extracted and purified DNA is then loaded into a
GeneDisc Plate designed for specific detection and/or quantification of microorganisms of interest. The GeneDisc Plate is then
placed into the GeneDisc Cycler for analysis. The analysis step
by real-time Polymerase Chain Reaction (RT-PCR) runs in as little
as an hour and the final results are displayed with no further
operator intervention.
Purification of DNA Samples
For specific applications, such as quantification of Legionella
in complex waters, DNA purification is a key step of sample
preparation. DNA purification enables:
Step 1: After cell lysis*, transfer lysate to silica column for DNA
binding using multichannel pipette provided.
• To reduce PCR inhibitions due to organic compounds from
samples
• To achieve highly accurate results.
The GeneDisc Ultra-Purifier ensures high quality DNA purification
based on the technology of DNA molecules adsorption to silica
and solvent washings. This protocol, applied to GeneDisc
methods for Legionella, was validated by NF VALIDATION
against NF T90-471 and ISO /TS 12869 standards.
Thus, sample preparation for GeneDisc method provides purified
DNA samples with a high recovery rate, ideal for quantitative
applications.
Step 2: Proceed to DNA washings.
Step 3: Elute DNA molecules.
DNA extracts are now purified and ready to be processed.
Transfer them to the GeneDisc Plate and analyze using the
GeneDisc Cycler.
* Lysis tube rack is supplied with GeneDisc Ultra-Lyser.
2
Technical Specifications
Ordering Information
Physical Dimension
Description
Part Number
GeneDisc Ultra-Purifier
EGDUP1A
Weight
Dimensions
(Height x WIdth x Depth)
10 kg (22 lb)
318 x 259 x 363 mm
(12.5 x 10.2 x 14.3 in)
References
Electrical Data
Electrical voltage
100-240 V AC
Maximum current
Power consumption
2.5 A
600 W
NF T 90-471 standard: Water quality—Detection and quantification of
Legionella and/or Legionella pneumophila by concentration and genic
amplification by real time polymerase chain reaction (qPCR)
ISO /TS 12869 standard: Water quality — Detection and quantification
of Legionella spp. and/or Legionella pneumophila by concentration and
genic amplification by quantitative polymerase chain reaction (qPCR)
Operating Conditions
Temperature
Relative Humidity
5 °C to 40 °C ( 41 °F to 104 °F)
10% to 95%, non-condensing
Usage Conditions
Temperature
15 °C to 30 °C ( 59 °F to 86 °F)
www.pall.com/genedisc
3
GEN 25/03 – 12/07
GEN 25/04 – 12/07
ANALYTICAL METHODS FOR WATER
www.afnor-validation.com
Visit us on the Web at www.pall.com/genedisc
Pall GeneDisc Technologies
25 Harbor Park Drive
Port Washington, NY 11050
+1 516 484 3600
telephone
+1 866 905 7255
toll free US
genedisc@pall.com
International Offices
Pall Corporation has offices and plants throughout the world in locations such as: Argentina, Australia, Austria,
Belgium, Brazil, Canada, China, France, Germany, India, Indonesia, Ireland, Italy, Japan, Korea, Malaysia, Mexico,
the Netherlands, New Zealand, Norway, Poland, Puerto Rico, Russia, Singapore, South Africa, Spain, Sweden,
Switzerland, Taiwan, Thailand, the United Kingdom, the United States, and Venezuela. Distributors in all major
industrial areas of the world. To locate the Pall office or distributor nearest you, visit www.pall.com/contact.
The information provided in this literature was reviewed for accuracy at the time of publication. Product data may
be subject to change without notice. For current information consult your local Pall distributor or contact Pall directly.
© 2015, Pall Corporation. Pall,
, and GeneDisc are trademarks of Pall Corporation. ® indicates a trademark
registered in the USA and TM indicates a common law trademark. Filtration.Separation.Solution.SM is a service
mark of Pall Corporation.
FBDSGDUPEN
November 2015