Managing Glyphosate-Resistant Weeds in Glyphosate-Resistant Crops Dr. Bill McCloskey Extension Weed Specialist * RR Flex cotton * RR alfalfa * RR Corn * Tree crops – lemons, pecans, pistachios, etc. * General farm weed control/sanitation * Over-reliance on glyphosate? * Problem of herbicide resistant weeds * Desert Ag Conference – May 2012 2 * July 2012: field after 3 sprays of 44 oz/A (1.5 lb ae glyphosate/A). Difficulty controlling Palmer amaranth in 2011; cotton/wheat double crop 3 * 4 * 5 Weeds too large when spray by grower * "*5>?02$#@&'$/$%&'()*)+&$#AA;)*/?50$ !"#$%&'()*+%#&,&-)&./&012'&13"4&"1(5&5"46$($0"&1778$(1+)%9&1%0& ()%+%*"&1-&4"#*814&$%-"4:18'&*%+8&514:"'-;& ! <):"&1(4)''&-5"&="80&$%&1&'()*+%#&71>"4%&():"4$%#&& -5"&14"1;& ! ?6'"4:"&1%0&4"()40@& – – – – –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reatment % Resistant Weeds in Population .0001 Excellent 1st Application .00143 Excellent 2nd Application .0205 Excellent 3rd Application .294 Excellent 5th Application 4.22 60.5 R4"0$-@&<$Y"&Z"J"8$("& @%6"7079%A6%/7/<*3<'"7#<B,%'' Weed Control 0 Application 4th Application Year 2 0 1 Excellent Failure /%%9',##-'#6'/%%9'"*3?'73'/#7-' L3"4&=4'-&1778$(1+)%9&-5"&4"'$'-1%-&$%0$:$0*18& R)%-4)8&$'&'+88&"X("88"%-9&6*-&& J$4'-&1778$(1+)%&)F&5"46$($0"&& '*4:$:"'&-5"&-4"1-C"%-&1%0&74)0*("'&'""0& 4"'$'-1%-&'""0&()%+%*"&-)&$%(4"1'"& -)&-5"&$%$+18&7)7*81+)%& !""#$%&'()*)+&$,&-)-./0*&$1/0/2&3&0.$4&--50$G$7$89::$!""#$#;;$,)2<.-$,&-&'=&+! .W& D'52'&--)50$5C$!&&+$,&-)-./0*&$ A""0&4"'$'-1%("&& 74)#4"''"'&8)#14$-5C$(1882& Treatment % Resistant Weeds in Population .0001 Excellent 1st Application .00143 Excellent 2nd Application .0205 Excellent 3rd Application .294 Excellent 5th Application 4.22 60.5 R4"0$-@&<$Y"&Z"J"8$("& Weed Control 0 Application 4th Application 3 Year 5 4 Excellent Failure I"46$($0"&4"'$'-1%("&(1%%)-&6"&4":"4'"0&$%& seed pool or seed bank in soil 1&741(+(18&+C"&F41C";&S%&C1%2&(1'"'9&-5"& '""0&7))8&$'&*%8$Y"82&-)&(51%#"&61(Y& R)%-4)8&C12&'+88&177"14&1(("7-168"9&6*-&-5"&'""0&7))8&$'& A""0&781%-'&1%0&'""0&7))8&14"& R)%-4)8&$'&'+88&:"42&#))09&6*-&& 6"(1*'"&-5"4"&$'&%)&=-%"''&7"%18-2;& 18C)'-&()C78"-"82&()C7)'"0&)F&-5"&4"'$'-1%-&-27"& 4"'$'-1%-&'""0&()%+%*"&-)&$%(4"1'"& %)G&C)'-82&5"46$($0"V4"'$'-1%-& !""#$%&'()*)+&$,&-)-./0*&$1/0/2&3&0.$4&--50$G$7$89::$!""#$#;;$,)2<.-$,&-&'=&+! ..& Robust annual – reproduces by seed Palmer amaranth seed production – 600,000 to 1.6 million seeds per large plant. An infestation of 1.6 plants/ft of crop row can produce 600 million seeds per acre. 12 Male Palmer amaranth Male flowers with yellow anther sacs * Female Palmer amaranth flowers 13 * 14 * 15 Male Palmer amaranth Male flowers with yellow anther sacs Female Palmer amaranth flowers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lick to close.] and 5"46$($0"'& ()%-4)8&B"1(5&5"46$($0"&$'&1(+:"&)%&-5"& -14#"-&G""0&)4&G""0'D& L&()C6$%1+)%&)F&-1(+('&4"0*("'&-5"&'"8"(+)%& 74"''*4"&$C7)'"0&62&1%2&'$%#8"&741(+(";& !""#$%&'()*)+&$,&-)-./0*&$1/0/2&3&0.$4&--50$G$7$89::$!""#$#;;$,)2<.-$,&-&'=&+! .,& * Apply integrated weed management practices including sanitation, tillage and crop rotation. * Use multiple herbicide modes-of-action with overlapping weed spectrums in rotation, sequences, or mixtures. * Use the full recommended herbicide rate and proper application timing for the hardest to control weed species present in the field. * Scout fields after herbicide application to ensure control has been achieved. Avoid allowing weeds to reproduce by seed or to proliferate vegetatively. * Monitor site and clean equipment between sites. * 18 Herbicides Mechanical Cultural Multiple herbicides with different mechanisms of action Tillage Crop rotation Mixes Pre-plant Plant population Sequences In-crop cultivation Row spacing Across seasons In-row weeding Planting date Post-harvest Fertilizer placement Hand-rogueing before seed set * 19 Cover crops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KN& Herbicides Mechanical Cultural Multiple herbicides with different mechanisms of action Tillage Crop rotation Mixes Pre-plant Plant population Sequences In-crop cultivation Row spacing Across seasons In-row weeding Planting date Post-harvest Fertilizer placement Hand-rogueing before seed set * 24 Cover crops * * * !"#$%$&&'"#()*)+,'-.#)/-,#,$01)234-56 23)4056,'7 -+./056,'7 23)40/,1* 21,53+08 -+./0/,1* 9: '()*+(, ! "! #! $! %! &!! * * Roundup Ready Flex Cotton – glyphosate * Roundup Ready Flex Pima Cotton * Liberty Link Cotton – glufosinate * GlyTol: Glufosinate and Glyphosate resistant cotton * Dicamba, glyphosate and glufosinate 3-gene stacked cotton varieties * Preplant Early POST topical Mid POST Post-direct Layby PD broadcast Tillage Dual Magnum Glyphosate Glyphosate Pendimethalin Prowl H20 Staple LX Staple LX Trifluralin Glyphosate Liberty/Ignite 280 Liberty/Ignite 280 Prometryn Staple LX Sandea Prowl H2O* Diruon Liberty/Ignite 280 Prometryn* Prometryn* Diuron* Diuron* Layby Pro* Layby Pro* Goal, GoalTender* Goal, GoalTender* *Preemergence soil activity Aim Aim Chateau* Chateau* ET ET Herbicides Mechanical Cultural Multiple herbicides with different mechanisms of action Tillage Crop rotation Mixes Pre-plant Plant population Sequences In-crop cultivation Row spacing Across seasons In-row weeding Planting date Post-harvest Fertilizer placement Hand-rogueing before seed set Cover crops * 30 * * Before cultivation After cultivation * * * Key components: Disk stabilizers to stop implement drift Adjust sway blocks to allow implement to run at correct depth. Implement must not rotate with respect to tractor. * * Cotton must be 12 inches tall with bark on the lower stem to use precision cultivation with in-row weeding. Crop must have a well developed tap root. * Can close cultivate on smaller row crops without in-row weeding. * Crop stem damage from moving soil remains a limitation. * Zero tolerance for Palmer amaranth flower (pollen) and seed production = SANITATION! * Remove weeds before they produce viable seed. * 37 Herbicides Mechanical Cultural Multiple herbicides with different mechanisms of action Tillage Crop rotation Mixes Pre-plant Plant population Sequences In-crop cultivation Row spacing Across seasons In-row weeding Planting date Post-harvest Fertilizer placement Hand-rogueing before seed set * 38 Cover crops * * Eliminate weed competition to optimize alfalfa stand * As a broadcast planted crop, alfalfa is fairly competitive against weeds particularly if the weeds are injured by herbicides * Herbicide use can stunt or injury alfalfa seedlings * Weed competition also stunts alfalfa seedlings * * 41 * Alfalfa production (RR and conventional varieties) * Plant in fall (mid-October through November) * Enables 2,4-DB applications (e.g. Butyrac 200) due to lower temps (temperature cut-off of 90 F) * Raptor and pursuit alone or tank mix better on winter weeds, possible resistance problem * Trifluralin granules or water run Prowl H2O * Treat berms with residual herbicide or use tillage to keep them clean * (Roundup where susceptible weed populations occur) * 42 * 43 * 44 * • Center pivot irrigation • Row spacing: 30 inch • Plot size: 20 x 30 feet • Herbicides applied at 3 leaf (5 in) and 9 leaf (30 in) growth stages * * * Any weed management program that use soil-applied herbicides followed by postemergence herbicides have less variability in weed control, crop yield, and net return compared with total postemergence programs. * Weed Management * Herbicide – proactive and reactive * Utilize multiple herbicide modes of action, including those with residual effects, before applying glyphosate and/or tank mixing another herbicide with glyphosate. * Apply herbicides at the recommended stage of weed growth as stated on the label. * Conventional herbicides can and should still be part of the overall weed management system in glyphosateresistant cropping systems. * Since glyphosate resistance may be controlled by more than one gene, it is important to use full label glyphosate rates. * Resistant Management Strategies and Practices * Glyphosate-resistant crops are a powerful tool to manage weeds but must be used as part of an integrated pest management (IPM) program. * IPM principles and practices include field scouting or other detection methods, weed identification, population monitoring, and treatments. * Weed IPM programs in glyphosate-resistant crops need to consider all available weed management options appropriate to the system. * Summary