Journal of the Science of Food and Agriculture J Sci Food Agric 87:2141–2146 (2007) Antioxidant activities of defatted sesame meal extracts in cooked turkey breast and thigh meats Seong-Chun Jo,1 Ki-Chang Nam,2 Byoung-Rok Min,2 Dong U Ahn2 and Seung-Cheol Lee1∗ 1 Department 2 Department of Food Science and Biotechnology, Kyungnam University, Masan 631-701, Korea of Animal Science, Iowa State University, Ames, Iowa 50011-3150, USA Abstract: The antioxidant properties of methanolic extract of raw and roasted (at 200 ◦ C for 60 min) defatted sesame-meal in turkey breast and thigh meat systems were determined. The TBARS values of turkey breast and thigh meat added with 0.1% of roasted defatted sesame-meal extract were 18.8% and 24.7%, respectively, lower than those of untreated controls after 5 days of storage. The turkey meats added with roasted defatted sesame-meal extract had higher a∗ and b∗ values than those of controls due to the browning effects occurred in sesame seeds during roasting. The amounts of volatile hydrocarbons (pentane, hexane, heptane, and octane) and carbonyls (propanal, butanal, pentanal, hexanal and heptanal) significantly decreased by the addition of roasted defatted sesame-meal extract. In particular, the amount of hexanal, the most predominant volatile compound in the cooked turkey meat, decreased by 74% and 83% in turkey breast and thigh meat, respectively. However, raw defatted sesame-meal extract did not show significant antioxidant activity in turkey meats. These results indicated that heat treatment of sesame-meal increased the antioxidant activities of methanolic extract of defatted sesame meal. 2007 Society of Chemical Industry Keywords: defatted sesame meal extracts; turkey meat; antioxidant activity INTRODUCTION Antioxidant activities have been reported from various plant sources such as grape seeds, pine bark, olive rape, rosemary, and cocoa by-products.1 – 3 Phenolic compounds are the main sources of their antioxidant activities, but most of them are present as covalently bound insoluble polymer forms.4,5 To obtain natural antioxidants from plants, therefore, it is necessary to find an effective processing method to liberate phenolic compounds.6,7 Several methods such as heat, farinfrared (FIR) radiation, fermentation, and protease treatments have been studied to liberate and activate low molecular weight natural antioxidants.8 – 11 Our preliminary studies showed that antioxidant activities of defatted sesame meal extracts increased as the roasting temperature of sesame seed increased, and the optimal heat treatment conditions for antioxidant activity were at 200 ◦ C for 60 min.12 It was also found that roasting sesame seeds at 200 ◦ C for 60 min significantly increased the total phenolic content, radical scavenging activity (RSA), reducing powers, and antioxidant activity of sesame meal extracts.12 Cooked meats are highly susceptible to lipid oxidation and produce off-odour volatiles, and the use of antioxidants is commonly required to retard quality deterioration by oxidative processes during storage. The need for natural antioxidants in food and meat industries is increasing because consumers ask for natural additives. Although a few plant extracts are widely used as safe antioxidants their activities are not as strong as synthetic antioxidants and the manufacturing cost is comparatively high.13 The objectives of this research were to determine the effect of roasted defatted sesame meal extract on lipid oxidation, production of volatile compounds, and colour changes in cooked turkey breast and thigh meat during refrigerated aerobic storage. EXPERIMENTAL Materials White sesame seeds (Sesamum indicum L.) were purchased from a local market in South Korea. 2Thiobarbituric acid (TBA) and trichloroacetic acid (TCA) were purchased from Sigma Chemical Co. (St Louis, MO, USA), and rosemary oleoresin from Ecom Manufacturing Corp. (Scarborough, ON, Canada). Methanol and ethanol were purchased from Duksan Pure Chemical Co. (Sungkok-Dong, Ansan, Kyungkido, Korea). Heat treatment Whole sesame seeds (20 g) were placed in a Pyrex Petri dish (8.0 cm diameter) and roasted in an electric ∗ Correspondence to: Seung-Cheol Lee, Division of Food Science and Biotechnology, Kyungnam University, Masan 631-701, Korea E-mail: sclee@kyungnam.ac.kr Contract/grant sponsor: Ministry of Commerce, Industry & Energy (MOCIE) Contract/grant sponsor: Korea Institute of Industrial Technology Evaluation & Planning (ITEP) through the Coastal Resource & Environmental Research Center (CRERC), Industry-Academic Cooperation Foundation, Kyungnam University, Korea (Received 24 May 2006; accepted 5 September 2006) Published online 18 June 2007; DOI: 10.1002/jsfa.2977 2007 Society of Chemical Industry. J Sci Food Agric 0022–5142/2007/$30.00 SC Jo et al. muffle furnace (Model DMF-802; Daeil Engineering, Masan, Korea) at 200 ◦ C for 60 min. After roasting, the seeds were allowed to cool to ambient temperature before extracting oil. Preparation of defatted sesame meal (DSM) and its methanolic extract Raw and roasted sesame seeds (20 g) were crushed and oil was extracted using an electric oil extractor (Model Do-9001; Donga Oscar Co., Gimhae, Korea). The remaining oil in residue was extracted with 100 mL of n-hexane by vigorous shaking in a threecycle shaker, filtered through a Whatman No. 1 filter paper (Whatman Int’l Ltd, Maidstone, UK) and the residue (DSM) was collected and dried at room temperature. The DSM (10 g) was extracted with 100 mL of methanol in a shake incubator overnight at room temperature and filtered through a Whatman No. 1 filter paper. The residue was re-extracted under the same conditions. The first and second extracts were pooled and filtered through a Whatman nylon membrane filter (0.2 µm, Millipore filteration kit, MA 01 730; Millipore Co., Bedford, UK). The methanol in the filtrate was evaporated using a rotary evaporator (Model Eyela N-1000; Tokyo Rikakikai Co., Tokyo, Japan). DSM1 is the methanol extract of raw defatted sesame meal, and DSM2 is the methanol extract of heat-treated (200 ◦ C for 60 min) defatted sesame meal. Preparation of turkey breast and thigh patties Turkey meat patties were prepared according to the method of Nam et al.14 Turkey breast and thigh meats from 16 different turkeys were randomly divided into four groups. Each group was ground separately through a 3 mm plate and used as a replication. For each replication, four different treatments were prepared using rosemary extracts and defatted sesame meal extracts: (1) control, no additive; (2) rosemary extract, 0.1%; (3) DSM1, 0.1%; (4) DSM2, 0.1%. DSM1 and DSM2 extracts were dissolved in ethanol (150 mg mL−1 ) before addition. The same amounts of ethanol were added to all treatments to minimise solvent effects. Each additive was added to ground turkey meat and mixed for 2 min in a bowl mixer (model KSM 90; KitchenAid Inc., St Joseph, MI). The mixed meats were ground again through a 3 mm plate to ensure even distribution of the additives. Turkey breast and thigh meat patties (∼40 g each) were prepared and individually packaged in oxygenimpermeable vacuum bags (9.3 mL O2 m−2 24 h−1 at 0 ◦ C; Koch, Kansas City, MO, USA). The meat samples were precooked in a 90 ◦ C water bath to an internal temperature of 80 ◦ C. After cooking, turkey patties were chilled in running cold water for 10 min, removed from the vacuum bags and repackaged individually in oxygen-permeable bags (polyethylene, 4 × 6 , 2 MIL, Associated Bag Co., Milwaukee, WI). The aerobically packaged samples were stored at 4 ◦ C. Lipid oxidation, colour and volatile compounds of the samples were determined at 0, 2 and 5 days of storage. 2142 Values for 2-thiobarbituric acid-reactive substances Lipid oxidation was determined by measuring TBARS content.15 Minced sample (5 g) was placed in a 50 mL test tube and homogenised with 15 mL of deionised distilled water (DDW) using a Brinkman polytron (type PT 10/35, Brikman Instrument Inc., Westbury, NY) for 15 s at high speed. The meat homogenate (1 mL) was transferred to a disposable test tube (13 × 100 mm), and butylated hydroxytoluene (7.2%, 50 µL) and thiobarbituric acid/trichloroacetic acid (20 mmol L−1 TBA and 15% (w/v) TCA) solution (2 mL) were added. The samples were vortex-mixed and then incubated in a 90 ◦ C water bath for 15 min to develop colour. After cooling for 10 min in cold water, the samples were mixed and centrifuged at 3000 × g for 15 min at 5 ◦ C. The absorbance of the resulting upper layer was read at 531 nm against a blank prepared with 1 mL of DDW and 2 mL of TBA/TCA solution. The amount of TBARS was expressed as milligram of malonedialdehyde (MDA) per kilogram of meat. Colour measurement CIE colour values were measured on the surface of samples using a LabScan colour meter (Hunter Associated Laboratories, Inc., Reston, VA) that had been calibrated against black and white reference tiles covered with the same packaging materials as used for the samples. The CIE L∗ (lightness), a∗ (redness) and b∗ (yellowness) values were obtained using an illuminant A (light source). Area view and port size were 0.625 cm and 1.0 cm, respectively. The values from four random locations of upper and bottom surfaces of patties were obtained, averaged, and used for statistical analysis. Analysis of volatiles compounds A dynamic headspace analysis was performed using a Solatek 72 multimatrix vial autosampler and a purge and trap concentrator 3100 (Tekmar–Dohrmann, Cincinnati, OH) connected to a gas chromatograph–mass spectrometer (GC–MS, Hewlett-Packard Co., Wilmington, DE) according to the method of Ahn et al.16 Minced sample (1 g) was placed in a 40 mL sample vial, the vial was flushed with helium gas (40 psi) for 3 s, and capped airtight with a Teflon fluorocarbon resin/silicone septum (IChem Co., New Castle, DE). The maximum storage of a sample before analysis in a refrigerated (4 ◦ C) loading tray was 2 h or less to minimise oxidative changes during the waiting period before the start of the analysis. The meat sample was purged with helium gas (40 mL min−1 ) for 14 min at 40 ◦ C. Volatiles were trapped using a Tenax/charcoal/silica column (Tekmar–Dohrmann) and desorbed for 2 min at 225 ◦ C, focused in a cryofocusing module (−80 ◦ C), and then thermally desorbed into a column for 60 s at 225 ◦ C. An HP-624 column (7.5 m, 0.25 mm i.d., 1.4 µm nominal), an HP-1 column (52.5 m, 0.25 mm i.d., 0.25 µm J Sci Food Agric 87:2141–2146 (2007) DOI: 10.1002/jsfa Antioxidant activities of sesame meal extracts nominal; Hewlett-Packard Co., Wilmington, DE), and an HP-Wax column (7.5 m, 0.25 mm i.d., 0.25 µm nominal) were connected using zero dead-volume connectors (J&W Scientific, Folsom, CA). Ramped oven temperature was used to improve volatile separation. The initial oven temperature of 0 ◦ C was held for 1.5 min. After that, the oven temperature was increased to 15 ◦ C at 2.5 ◦ C min−1 , increased to 45 ◦ C at 5 ◦ C min−1 , increased to 110 ◦ C at 20 ◦ C min−1 , and then increased to 170 ◦ C at 10 ◦ C min−1 and held for 2.25 min at that temperature. A constant column pressure at 20.5 psi was maintained. The ionisation potential of the mass selective detector (model 5973; Hewlett-Packard Co.) was 70 eV, and the scan range was m/z 19.1–350. Identification of volatiles was achieved by comparing mass spectral data of samples with those of the Wiley library (Hewlett-Packard Co.). Standards, when available, were used to confirm the identification by the mass selective detector. The area of each peak was integrated using ChemStation software (Hewlett-Packard Co.) and the total peak area (total ion counts ×104 ) was reported as an indicator of volatiles generated from the meat samples. Statistical analysis The experimental design was to determine the effects of defatted sesame meal extracts and storage time on lipid oxidation, volatile compounds, and colour of the turkey breast and thigh meat patties. Analysis of variance was conducted according to the procedure of the General Linear Model using SAS software, 1995.17 Student–Newman–Keuls’ multiple-range tests were used to compare the significant differences among the mean values of treatments (P < 0.05). Mean values and standard error of the means (SEM) were reported. RESULTS AND DISCUSSION TBARS values in cooked turkey breast and thigh meats TBARS have been used to quantify malondialdehyde (MDA) in meat due to lipid oxidation. Turkey breast and thigh meats added with the extract from roasted defatted sesame meal (DSM2) had lower TBARS values than the non-added control and had similar TBARS values to rosemary extractadded ones (Table 1). Rosemary extract contains high phenolic compounds and showed significant antioxidant activities in foods.18 The TBARS value of raw defatted sesame meal (DSM1) was not different from that of control. Cooked turkey breast meat showed lower initial lipid oxidation than thigh meat. Addition of DSM and rosemary extract also showed lower initial lipid oxidation in cooked turkey breast than thigh meat. As storage time increased, overall lipid oxidation was drastically accelerated due to structural damage in muscle fibres by cooking and aerobic storage conditions.19 However, incorporation of DSM2 to turkey breast and thigh meat reduced TBARS values J Sci Food Agric 87:2141–2146 (2007) DOI: 10.1002/jsfa Table 1. TBARS values (mg MDA kg−1 of meat) of cooked turkey breast and thigh meats added with raw defatted sesame meal extract (DSM1), rosemary extract (RE), or roasted defatted sesame meal extract (DSM2) during refrigerated storage Storage (day) Control RE DSM1 DSM2 SEM Turkey breast meat 0 2.46az 2 4.14ay 5 8.25ax SEM 0.40 0.80bz 2.61by 6.90bx 0.17 1.57abz 3.76ay 7.71ax 0.20 0.69bz 2.92by 6.70bx 0.09 0.35 0.14 0.20 Turkey thigh meat 0 2.43az 2 7.00ay 5 14.60ax SEM 0.28 0.79cz 4.13cy 11.98bx 0.21 1.20bz 4.16cy 11.00bx 0.19 0.08 0.14 0.54 2.48az 6.25by 15.56az 0.52 a–c Different letters within a row of the same meat patties are significantly different (P < 0.05), n = 4. x – z Different letters within a column are significantly different (P < 0.05), n = 4. Table 2. Typical off-odor volatiles profile of cooked turkey meat added with rosemary extract (RE), raw defatted sesame meal extract (DSM1), or roasted defatted sesame meal extract (DSM2) at 0 day Total ion counts ×104 Compound Control RE DSM1 DSM2 SEM 567b 225b 122b 230b 665b 72c 145b 186bc 126b 0d 0c 87c 301 13 22 34 526b 34 764b 5399d 289d 0c 0 1397b 22 442b 2036b 0c 0 1118b 14 334c 812c 42 31 158 979 114 Turkey breast meat Hydrocarbons Pentane 4931a Hexane 1742a Heptane 795a Octane 765a Carbonyls Propanal 4504a Butanal 90 Pentanal 4596a Hexanal 40 944a Heptanal 3197a Turkey thigh meat Hydrocarbons Pentane 0 Heptane 78 Octane 164 Carbonyls Propanal 0b Pentanal 1310 Hexanal 28 048a Heptanal 497 462 155 202 1410a 957 11 575b 315 0 70 131 208b 1110 25 736a 461 0 21 110 0b 518 10 333b 200 132 53 27 166 179 3050 77 a–d Different letters within a row of the same meat patties are significantly different (P < 0.05), n = 4. by 19% and 25% to their respective control after 5 days of storage. DSM1-added turkey meats did not show as low TBARS values as DSM2. Therefore, roasted defatted sesame meal (DSM2) had greater antioxidant activity than raw defatted sesame meal (DSM1) in cooked turkey breast and thigh meats. Our previous study12 indicated that roasting sesame seeds at 200 ◦ C for 60 min significantly increased the content of phenolic compounds in DSM2 from 35.6 µmol L−1 2143 SC Jo et al. to 87.4 µmol L−1 , DPPH radical scavenging activity from 34.01% to 82.14%, reducing power from 0.182 to 0.660, and induction of lipid peroxidation evaluated by Rancimat method from 0.18 h to 1.09 h, respectively, compared with non-roasted DSM1. It was also shown that several low-molecular-weight phenolic compounds such as 2-methoxyphenol, 4-methoxy3-methylthio-phenol, 5-amino-3-oxo-4-hexenoic acid, 3,4-methylenedioxyphenol (sesamol), 3-hydroxy benzoic acid, 4-hydroxy benzoic acid, vanillic acid, filicinic acid, and 3,4-dimethoxy phenol were newly formed in the DSM2. Thus, the phenolic compounds produced in DSM2 should have increased the antioxidant activity in cooked turkey breast and thigh meats. Inhibition of off-odour volatiles Production of warmed-over flavour is the most critical problem in cooked meat and the role of antioxidants is important in minimising oxidative changes in precooked meat during storage. The typical off-odour volatiles of cooked turkey meat during storage for 0, 2 and 5 days were identified (Tables 2, 3 and 4). When volatile compounds related to lipid oxidation were compared, volatile hydrocarbons (pentane, hexane, heptane and octane) and carbonyls (propanal, pentanal, hexanal and heptanal) were significantly decreased by the addition of DSM2 or rosemary Table 3. Typical off-odour volatiles profile of cooked turkey meat added with rosemary extract (RE), raw defatted sesame meal extract (DSM1), or roasted defatted sesame meal extract (DSM2) at 2 days Table 4. Typical off-odour volatiles profile of cooked turkey meat added with rosemary extract (RE), raw defatted sesame meal extract (DSM1), or roasted defatted sesame meal extract (DSM2) at 5 days Total ion counts ×104 Compound Control RE DSM1 DSM2 SEM 3859bc 701b 520c 571b 4789b 781b 731b 615b 3161c 434b 433c 401b 392 96 61 61 9283c 588b 11 579c 78 489b 2413c 10 906b 911ab 14 431b 89 975a 3818b 9684c 1221a 10 372c 71 917b 2250c 356 105 694 3301 246 2843bc 845b 546a 469a 3109b 913b 475ab 425a 2410c 738c 508ab 329b 168 29 33 15 9037b 9975b 610 567 10 604b 12 532ab 76 951b 73 843b 5747b 5511b 9526b 919 10 242b 75 448b 6030b 383 115 893 3647 319 Turkey breast meat Hydrocarbons Pentane 7320a Hexane 1676a Heptane 1147a Octane 1048a Carbonyls Propanal 12 301a Butanal 965ab Pentanal 16 970a Hexanal 96 551a Heptanal 5176a Turkey thigh meat Hydrocarbons Pentane 3732a Hexane 1111a Heptane 402b Octane 417a Carbonyls Propanal 13 165a Butanal 688 Pentanal 14 433a Hexanal 90 841a Heptanal 7338a a–c Different letters within a row of the same meat patties are significantly different (P < 0.05), n = 4. Total ion counts ×104 Compound Control RE DSM1 DSM2 SEM Turkey breast meat Hydrocarbons Pentane 4955 Hexane 858 Heptane 604 Octane 559 Carbonyls Propanal 5796 Butanal 315b Pentanal 6796 Hexanal 57 133a Heptanal 2736a 2332 438 277 345 4328 801 608 513 3172 529 415 309 720 173 105 168 4154 189b 4121 38 438b 676b 6701 351b 7119 53 770ab 2074a 1752c 707b 240b 317b 3220b 797b 446ab 389b 1752c 534b 393ab 270b 264 87 68 40 4630c 163b 4310b 42 805c 2536c 6154b 266b 7016a 53 216ab 4492b 5644b 740a 5131b 46 866bc 3381c 264 37 390 2442 325 5661 1070 915a 85 5483 924 45 443ab 4336 897b 354 Turkey thigh meat Hydrocarbons Pentane 4060a Hexane 1163a Heptane 623a Octane 518a Carbonyls Propanal 7136a Butanal 293b Pentanal 7363a Hexanal 58 051a Heptanal 5831a a – c Different letters within a row of the same meat patties are significantly different (P < 0.05), n = 4. 2144 extracts. Hexanal was the most predominant volatile compound in control meat, and DSM2 decreased hexanal content to 60% of control at the beginning of storage (Table 1). The amount of hexanal was highly correlated with TBARS value20,21 in cooked meats and could be used as a good indicator for lipid oxidation. DSM2-treated turkey breast and thigh meats had lower level of volatile aldehydes than those added with DSM1, showing that the antioxidant activity of DSM2 was greater than that of DSM1. After 2 days of storage, the hexanal contents of turkey breast and thigh controls increased by 1.4 and 2.1 times of day 0, respectively (Table 3). DSM2 treatment had as strong an antioxidant activity as rosemary extract. No significant difference in the amounts of most volatile aldehydes between the rosemary extract and DSM2 on turkey breast and thigh was observed. At day 5, antioxidant effects were mainly found in the rosemary extract and DSM2 treatments (Table 4). DSM2 reduced hexanal content of turkey breast and thigh meats to 74% and 83% of the control, which was statistically same effect as rosemary extract. These results indicated that the antioxidant effects of rosemary extract and DSM2 could be maintained for 5 days under aerobic storage conditions. J Sci Food Agric 87:2141–2146 (2007) DOI: 10.1002/jsfa Antioxidant activities of sesame meal extracts Table 5. Colour values of cooked turkey breast and thigh meat added with rosemary extract (RE), raw defatted sesame meal extract (DSM1), or roasted defatted sesame meal extract (DSM2) during refrigerated storage Turkey breast meat Storage (day) Control RE 83.63a 83.85 83.42ab 0.45 82.45a 82.75 82.30ab 0.42 5.62bx 4.77cy 4.31bz 0.13 5.77bx 5.24by 4.37bz 0.10 DSM1 DSM2 Turkey thigh meat SEM Control RE DSM1 DSM2 SEM L value 0 2 5 SEM 83.37a 83.67 83.82a 0.40 81.08by 82.60x 81.69bxy 0.37 0.40 0.42 0.44 65.24 66.13 65.57 0.50 65.07 65.96 65.74 0.52 65.14 66.01 65.92 0.45 64.79 65.20 64.26 0.51 0.60 0.38 0.48 5.77bx 4.65cy 3.90cz 0.08 7.01ax 6.32ay 5.51az 0.13 0.09 0.16 0.08 11.16x 10.04by 9.59by 0.16 11.06x 10.83ax 9.96by 0.23 10.93x 10.29by 9.58bz 0.18 11.39x 11.02axy 10.64ay 0.17 0.16 0.17 0.23 17.09bx 17.68bx 15.97by 0.22 20.40ax 19.98ax 18.76ay 0.33 0.28 0.24 0.28 21.84by 21.94b 23.18bcx 22.11c 21.81ay 21.65a 0.25 0.41 21.18by 22.33bcx 21.58axy 0.31 23.25ax 23.44ax 21.97ay 0.34 0.11 0.30 0.34 a value 0 2 5 SEM b value 0 2 5 SEM 16.69b 17.01b 16.78b 0.28 17.50b 17.03b 16.82b 0.24 a–c Different letters within a row of the same meat patties are significantly different (P < 0.05), n = 4. x – z Different letters within a column of the same color parameter are significantly different (P < 0.05), n = 4. Colour changes by defatted sesame meal extract Due to the characteristic brown colour of defatted sesame meal extract, redness (a∗ ) and yellowness (b∗ ) values drastically increased in cooked turkey breast and thigh meat. L∗ values, however, were not much changed (Table 5). This could be detrimental because consumers usually expect the colour of cooked poultry breast meat to be white. To increase the possibility of defatted sesame meal extracts as a food additive, therefore, a way to decrease the colour intensity is needed. After 5 days of refrigerated storage under aerobic conditions, the a∗ values of cooked turkey breast and thigh decreased significantly compared with the values at 0 day at all treatments. However, the a∗ and b∗ values were still higher in DSM2-added samples than others. The reason could be the increased brown colour intensity of DSM2 during the heat treatment. CONCLUSIONS Heat treatment of sesame seeds increased the antioxidant activities of defatted sesame meal in cooked turkey breast and thigh meat. However, an increased brown colour in roasted defatted sesame meal increased the redness of cooked turkey breast meat. If defatted sesame meal is to be used as a natural antioxidant, therefore, efficient ways of concentrating antioxidant components or removing unnecessary colour compounds is critical. ACKNOWLEDGEMENT This study was supported by Ministry of Commerce, Industry & Energy (MOCIE) and Korea Institute of Industrial Technology Evaluation & Planning (ITEP) through the Coastal Resource & Environmental Research Center (CRERC), Industry-Academic J Sci Food Agric 87:2141–2146 (2007) DOI: 10.1002/jsfa Cooperation Foundation, Kyungnam University, Korea. S.C. Jo received scholarship from the Brain Korea 21 Program. 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J Sci Food Agric 87:2141–2146 (2007) DOI: 10.1002/jsfa