Appendix A - IACUC Protocol IACUC #: Three-Year Progress Report For Office Use Only University of California, Irvine Institutional Animal Care and Use Committee Protocol #: Lead Researcher: This appendix must be completed and submitted with all three-year renewal submissions for ongoing projects using live vertebrate animals. (See the Animal Care and Use website for more information.) Provide the following information about the progress that was made during the last 3-year period of this study: 1. Indicate how many animals of each species were used in the last year (since your last annual continuation report): To look up the number of animals used, please go to the Animal Numbers Web Reporting website. USDA Pain Categories B Animals held only for breeding and not used for any research purpose. This includes founder/current breeder animals, replacement breeders, and animals produced but not used. C Momentary or no pain, distress or discomfort (e.g., needle stick) D Procedures reasonably considered painful or likely to cause discomfort or distress, but alleviated with analgesics, anesthesia or timely euthanasia. E Unrelieved pain, distress or discomfort (To add more rows, place the cursor in the last cell on the lower right and press key “Tab”) Species USDA Pain Category (B, C, D, or E) # of Animals Used 2. Indicate the total number of animals of each species were used in the last 3-year approval period (since the last full committee review of the project): To look up the number of animals used, please go to the Animal Numbers Web Reporting website. (To add more rows, place the cursor at the last cell on the lower right and press key “Tab”) Species USDA Pain Category (B, C, D, or E) # of Animals Used 3. Summarize the progress made on the project during the last 3-year approval period. In addition to the summary, you may cite abstracts, presentations and/or publications wherever possible (copies of publications may be attached if you wish). UCI Institutional Animal Care and Use Committee Version 2.0 IACUC Protocol Application - Appendix A Page 1 of 2 4. Discuss any unanticipated problems with animal health, behavior or well-being, including adverse events, and describe the actions that were taken to prevent recurrence: 5. Provide scientific justification for continuing this project: UCI Institutional Animal Care and Use Committee Version 2.0 IACUC Protocol Application - Appendix A Page 2 of 2