Product Data
AcroPrep™ Advance Filter Plates
Next generation 96-well filter plate
offers improved consistency and
reproducibility
• New well design increases well-to-well consistency
for sample processing and recovery.
• New sealing technology minimizes sample weeping
during incubations.
• Redesigned outlet tips minimize the formation
of hanging drops, reducing the chance of crosscontamination following filtration.
• Optimized membrane configurations for assays,
such as multiplexing and neonatal screening.
• Individually sealed membranes and biologically
inert housing minimizes the possibility of sample
cross-contamination or loss.
• Designed to meet SBS/ANSI guidelines, ensuring
compatibility with standard processing equipment.
• Smooth top surface, textured window for
labeling, and A1 corner notch allow for easy
identification.
Specifications
Materials of Construction
Filter Media: Glass fiber (borosilicate glass), Omega™
(modified PES), PP/PE non-woven (polypropylene,
polyethylene), and Supor® (PES) membranes
Plate Housing: Polypropylene
Lid: Polystyrene
Dimensions
Length: 12.78 cm (5.03 in.)
Width: 8.55 cm (3.36 in.)
Height (with lid): 1.75 cm (0.69 in.)
Height (without lid): 1.44 cm (0.56 in.)
Well Bottom Area: 0.25 cm2
Maximum Well Volume
350 µL
Recommended Working Volume
300 µL
Recommended Operating Vacuum
≥ 25.4 cm Hg (10 in. Hg)
Recommended Centrifugal Force
1,000-3,000 x g
Applications
The AcroPrep Advance filter plate portfolio includes a variety
of membrane types and pore sizes to provide the best
possible performance for a variety of sample preparation
applications. For critical applications, such as multiplexing
and neonatal screening, we have specifically optimized plates
based on the assay fractionation requirements. In addition,
we have a portfolio of plates available for applications such as
general filtration, lysate clarification, nucleic acid binding, and
protein fractionation.
Applications
Neonatal
Screening
Multiplexing
Nucleic
Acids
Sample
Prep
Proteins
Cell-Based
Bead-Based
Continuous
Flow
Microplate
Binding
Ultrafiltration
Fractionation
General Aqueous
Filtration
Prefiltration
30-40 µm
PP/PE, Supor
Multiplex
Assays
Neonatal
Plate
Biotinidase
Assay
Glass Fiber
Omega
Supor
Supor
Glass/Supor
PN 8079
PN 8060
PN 6008031
PN 6008033
PN 6008034
PN 6008035
PN 6008036
PN 6008027
PN 6008019
PN 6008029
PN 6008039
PN 8049
PN 6008039
PN 6008019
PN 6008029
PN 6008039
PN 6008040
PN 6008075
Performance
Hold-up Volume – Maximizing the amount of available
sample ensures more reliable results and yields more
material available for additional analysis. AcroPrep Advance
filter plates have an improved well design to minimize sample
hold-up, improve sample recovery, and maximize yield.
Figure 1
Lower Hold-Up Volume Improves Sample Recovery
30
25
Hold-Up Volume (µL/well)
Our next generation AcroPrep Advance filter plate was
developed with a strong understanding of the most stringent
performance requirements. The result is a plate that provides
improved performance and increased consistency for key
assay parameters. The AcroPrep Advance filter plate
incorporates new membrane sealing technology which
improves sample recovery, filtration rates, and lowers holdup volumes (Figure 1). The newly configured outlet tips
minimize sample weeping during incubations, reduce sample
loss, and minimize hanging drops on the outlet tips following
filtration (Figure 2, 3). Each of these design attributes
improves the performance reproducibility of the plate from
well-to-well, plate-to-plate, and lot-to-lot.
20
15
10
5
0
AcroPrep
Advance
Competitor M
Multiple plates of each type were evaluated for hold-up volume using a
solution of Vitamin B12. After all wells were evacuated, a wash fraction
was collected into a solid bottom plate and read at OD 550 for
concentration of Vitamin B12. AcroPrep Advance: total 9 plates were
tested (3 plates/lot). Competitor M: total 3 plates were tested. All testing
used 96 wells/plate.
Performance (continued)
Figure 2
Reduction of Hanging Drops Reduces Potential
Cross Contamination
Hanging Drop (µL/plate)
250
200
150
Figure 3
Reduced Weeping Minimizes Sample Loss and Provides
for Extended Incubations
Aqueous Solution
6
Average Weeping Wells
Hanging Drops – To help maintain the integrity of samples
and reduce the potential for cross contamination, the outlet
tips of the plate have been redesigned to nearly eliminate the
presence of hanging drops following filtration.
4
2
0
1 hr.
100
2 hr.
3 hr.
Time
AcroPrep Advance
50
0
Hanging drops were measured by evacuating wells of fluid, weighing the
plate, and then blotting and re-weighing the plate. Three plates of each
type were tested and the averages calculated.
Weeping – Filter plates should be able to hold sample for
extended periods of times. Weeping, or leaking of fluid from
wells, can occur during incubation periods or after multiple
filter-wash-incubation steps. Pall has engineered the new
AcroPrep Advance filter plates to minimize weeping when
using a variety of fluids including aqueous samples, organic
solvents, and detergent-containing solutions.
24 hr.
Competitor M
Organic Solvent
10
Average Weeping Wells
Competitor M
8
6
4
2
0
1 hr.
2 hr.
3 hr.
Time
AcroPrep Advance
8 hr.
24 hr.
Competitor M
Detergent Solution
10
Average Weeping Wells
AcroPrep
Advance
8 hr.
8
6
4
2
0
1 hr.
2 hr.
3 hr.
Time
AcroPrep Advance
8 hr.
24 hr.
Competitor M
Filter plates were filled with 200 µL of the indicated solutions and
incubated over receiver plates for 24 hours. Receiver plates were
checked for the presence of fluid at 1, 2, 3, 8, and 24 hour intervals.
Three plates of each variety were evaluated and the averages calculated.
www.pall.com/lab
Ordering Information
AcroPrep Advance Filter Plates
Part Number
6008019
6008029
6008039
6008027
6008031
6008033
6008034
Description
0.2 µm Supor membrane
0.45 µm Supor membrane
1.2 µm Supor membrane
30-40 µm PP/PE non-woven media
1 µm glass fiber
3K, Omega membrane
10K, Omega membrane
Pkg
100/pkg
100/pkg
100/pkg
100/pkg
100/pkg
100/pkg
100/pkg
Part Number
6008035
6008036
6008040
6008075
8049*
8060*
8079*
Description
30K, Omega membrane
100K, Omega membrane
3 µm glass fiber/1.2 µm Supor membrane
3 µm glass fiber/0.2 µm Supor membrane
For multiplex assays
For biotinidase assays
For neonatal screening
Pkg
100/pkg
100/pkg
100/pkg
100/pkg
10/pkg
10/pkg
10/pkg
*Available in bulk packs.
Complementary Products
• AcroPrep 384-well Filter Plates for superior performance
in high throughput sample preparation applications.
• AcroWell™ 96-well Filter Plates with BioTrace™ NT and
BioTrace PVDF Membranes exhibit high binding capacities
for proteins and nucleic acids.
• Centrifugal Devices for processing the following
sample volumes:
Device
Nanosep® Device
Microsep™ Device
Macrosep® Device
Jumbosep™ Device
Sample Volume
< 0.5 mL
0.5-3.5 mL
3.5-15 mL
15-60 mL
• Nanosep MF (Microfiltration) Centrifugal Devices are
available with Bio-Inert® and GHP membranes for low
protein binding and high recoveries in applications, such
as particulate removal prior to sample analysis (GHP
product is HPLC grade) and removal of precipitates.
• BioTrace, Biodyne®, and FluoroTrans® Transfer
Membranes offer precise performance and compatibility
with nearly every detection system available.
• AcroSep™ Chromatography Columns and Resins for
the purification of biomolecules and compounds. Available
chemistries include affinity, ion exchange, size exclusion,
mixed-mode, and hydroxyapatite.
Visit us on the Web at www.pall.com/oem
E-mail us at LabCustomerSupport@pall.com
Pall Life Sciences
600 South Wagner Road
Ann Arbor, MI 48103-9019 USA
800.521.1520 USA and Canada
(+)800.PALL.LIFE Outside USA and Canada
734.665.0651 phone
734.913.6114 fax
Australia – Cheltenham, VIC
Tel: 03 9584 8100
1800 635 082 (in Australia)
Fax: 1800 228 825
Austria – Wien
Tel: 00 1 49 192 0
Fax: 00 1 49 192 400
Brasil – São Paulo, SP
Tel: +55 11 5501 6000
Fax: +55 11 5501 6025
Canada – Ontario
Tel: 905-542-0330
800-263-5910 (in Canada)
Fax: 905-542-0331
Canada – Québec
Tel: 514-332-7255
800-435-6268 (in Canada)
Fax: 514-332-0996
800-808-6268 (in Canada)
China – P. R., Beijing
Tel: 86-10-6780 2288
Fax: 86-10-6780 2238
France – St. Germain-en-Laye
Tel: 01 30 61 32 32
Fax: 01 30 61 58 01
Lab-FR@pall.com
Germany – Dreieich
Tel: 06103-307 333
Fax: 06103-307 399
Lab-DE@pall.com
India – Mumbai
Tel: 91 (0) 22 67995555
Fax: 91(0) 22 67995556
Italy – Buccinasco
Tel: +3902488870.1
Fax: +39024880014
Japan – Tokyo
Tel: 03-6901-5800
Fax: 03-5322-2134
Korea – Seoul
Tel: 82-2-560-8711
Fax: 82-2-569-9095
Malaysia – Selangor
Tel: +60 3 5569 4892
Fax: +60 3 5569 4896
Poland – Warszawa
Tel: 22 510 2100
Fax: 22 510 2101
Russia – Moscow
Tel: 5 01 787 76 14
Fax: 5 01 787 76 15
Singapore
Tel: 65 6 389-6500
Fax: 65 6 389-6501
South Africa –
Johannesburg
Tel: +27-11-2662300
Fax: +27-11-3253243
Spain – Madrid
Tel: 91-657-9876
Fax: 91-657-9836
Sweden – Lund
Tel: (0)46 158400
Fax: (0)46 320781
Switzerland – Basel
Tel: 061-638 39 00
Fax: 061-638 39 40
Taiwan – Taipei
Tel: 886 2 2545 5991
Fax: 886 2 2545 5990
Thailand – Bangkok
Tel: 66 2937 1055
Fax: 66 2937 1066
United Kingdom –
Farlington
Tel: 02392 302600
Fax: 02392 302601
Lab-UK@europe.pall.com
© 2010, Pall Corporation. Pall,
, AcroPrep, AcroSep, AcroWell, Biodyne, Bio-Inert, BioTrace, FluoroTrans,
Jumbosep, Macrosep, Microsep, Nanosep, Omega, and Supor are trademarks of Pall Corporation. ® indicates
a trademark registered in the USA. Filtration.Separation.Solution.SM is a service mark of Pall Corporation.
1/10, 1.7k, AA GN09.3240
PN33567