Making It Simple
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dedicated
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Making It Simple
In the ever-changing environment of Life Sciences, proven
technologies, high quality products, and reproducible results
have become standards in sample preparation and detection
technologies. Pall Life Sciences stands apart with products
that are a direct result of smart thinking and consideration of
customer needs.
At Pall Life Sciences, we bring together membranes with
superior performance and devices designed to maximize
processing accuracy and speed. We develop and produce
thousands of different membrane chemistries for a multitude
of applications. Our impressive offering of membrane and
device platforms enables us to supply the best combination
for your filtration, separation, purification or detection
application. This brochure will help you to select from only
a few of our sterile filtration, molecular separation, and transfer
and immobilization products. If you do not see the item you
are looking for, just ask us or visit our web site at www.pall.com/lab.
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Sterile Acrodisc® Syringe Filters
Superior flow rates and higher
throughputs than competitive devices
Special Features
Featured Products
• Fast flow rates
Acrodisc Syringe Filters with Supor ® Membrane
• High throughputs
• Low protein binding to minimize sample loss
• Built-in prefilter for increased throughput of
difficult-to-filter liquids (viscous or particulate)
• 100% integrity tested
Pall PN
VWR Cat. No.
Description
Packaging
4612
28143-310
0.2 µm, 25 mm
50/pkg
4614
28143-312
0.45 µm, 25 mm
50/pkg
4618
28143-314
0.8 µm, 25 mm
50/pkg
4652
28143-350
0.2 µm, 32 mm
50/pkg
• Easy to use luer-lok fittings
• Filter volumes from 10 – 150 mL
Applications
Acrodisc PF Syringe Filter
Pall PN
VWR Cat. No.
Description
Packaging
• Filtration of viral suspensions
4187
28144-009
0.8/0.2 µm, 25 mm
50/pkg
• Clarification of biological, protein, enzyme,
probe and hybridization buffers and other
aqueous samples
4658
28139-702
0.8/0.2 µm, 32 mm
50/pkg
• Filtration of cell and tissue culture media
• Filtration of aqueous/organic solutions
• Filtration where low protein binding is desired
• Choose 0.2 µm for sterile filtration and
ultra-cleaning and 0.45 µm for particle
removal and clarification
3
VacuCap® and VacuCap PF
Sterile Vacuum Filtration Devices
Innovative bottle-top filters for fast vacuum
filtration of 100 mL to 5 L of aqueous solutions
Instructions
Special Features
1. VacuCap device is
• Supor® membrane provides high flow rates
• High throughputs
• Built-in prefilter prevents clogging and increases throughput
of viscous and high-particulate solutions
removed from the sterile
packing and placed on
the receiving bottle with
gasket seated on the rim
of the receiving bottle.*
• VacuCap 60 filters 100 mL - 1 L,
VacuCap 90 filters 1 - 5 L
2.The feed tubing is connected
• Draws directly from mixing reservoir
• Eliminates possibility of contamination from transfer
steps–filters directly into container
• Patented small design accepts a variety of collection vessels
and reduces storage space and waste
to the port marked “INLET”
on the top of the VacuCap
device. The opposite end of
the feed tubing is placed in
the vessel from which the
unfiltered fluid will be drawn.
3. Vacuum tubing is
Applications
• Vacuum-driven filtration of cell and tissue culture media, microbiological media, aqueous solutions, protein solutions, and buffers
• Prefiltration or clarification of aqueous solutions
connected to the port
marked “VACUUM”
on the top of the
VacuCap device.
Refer to product insert
for safety precautions.
• PF version useful for sterilization of hard-to-filter solutions
4. To begin filtration, the vacuum
Featured Products
VacuCap 60 Devices
Pall PN
VWR Cat. No.
Description
Packaging
4632
28143-332
0.2 µm, 60 mm
10/pkg
4638
28139-704
0.8/0.2 µm, 60 mm
10/pkg
VacuCap 90 Devices
4
Pall PN
VWR Cat. No.
Description
Packaging
4622
28143-315
0.2 µm, 90 mm
10/pkg
4628
28139-706
0.8/0.2 µm, 90 mm
10/pkg
is started while holding the
VacuCap device securely onto
the filtrate container. When
sufficient vacuum is created,
the VacuCap device will seal
securely to the container top
and fluid will be drawn into
the filtrate container.
5.When filtration is complete,
the vacuum pump is
switched off and the
vacuum inside the
receiving container is
allowed to dissipate.
Refer to the product insert
for complete instructions.
* Always use sterile bottles that are designed to be used with
a vacuum. We strongly recommend a safety shield during
vacuum procedures.
Nanosep® and Nanosep MF
Centrifugal Devices
Simple, reliable concentrating and desalting of
50 to 500 µL in just 5 to 15 minutes
Special Features
• Rapid processing of samples
Featured Products
Nanosep Centrifugal Devices with OmegaTM Membrane
• Typical recoveries are greater than 90%
Pall PN
VWR Cat. No.
Description
Packaging
OD003C33
29300-606
3K, gray
24/pkg
• Constructed of low binding polypropylene
OD010C33
29300-608
10K, blue
24/pkg
• Ultrasonically welded seals prevent bypass or
seal failure
OD030C33
29300-610
30K, red
24/pkg
OD100C33
29300-612
100K, clear
24/pkg
OD300C33
29300-614
300K, orange
24/pkg
ODM02C33
29300-638
0.2 µm, aqua
24/pkg
ODM45C33
29300-640
0.45 µm, wildberry 24/pkg
• A wide range of MWCOs color-coded for
easy identification
• Fit standard centrifuge rotors that accept
1.5 mL tubes
Applications
• Concentrate, purify, and desalt peptides,
proteins, oligonucleotides, DNA, and RNA
• Clean up labeling and PCR reactions
• Isolate DNA from agarose gel slices
• Separate proteins, oligonucleotides, and RNA
from acrylamide gels
• Sample preparation for HPLC analysis
5
BioTrace™ PVDF, BioTrace NT,
and Biodyne® Transfer Membranes
Membranes for transfer and immobilization
Special Features
for BioTrace PVDF
Transfer Membranes
Special Features
for BioTrace NT
Transfer Membranes
• Great general purpose protein transfer membrane
• Strong and durable, less likely to tear
or crack than competitor nitrocellulose
• Low background with chemiluminescent detection systems
• 100% pure nitrocellulose, no support fabrics
to interfere with signal generation
• Strong chemical resistance
• No discoloration
• High binding capacity for proteins and
nucleic acids
• High strength
• Lower protein burn-through than competitors
in electrophoretic transfers
Applications for
BioTrace PVDF
Transfer Membranes
Applications
for BioTrace NT
Transfer Membranes
• Western transfers
• Protein dot blots
• Western blots
• Colony/plaque lifts
Featured Products
• Southern/northern blots
• Protein/nucleic acid dot blots
BioTrace PVDF Transfer Membrane
Pall PN
VWR Cat. No.
Description
Packaging
66542
28148-750
20 x 20 cm sheets
10/pkg
66543
28148-752
30 cm x 3 m roll
1/pkg
BioTrace NT (Nitrocellulose) Transfer Membrane
6
Pall PN
VWR Cat. No.
Description
Packaging
66489
27377-000
20 x 20 cm sheets
10/pkg
66485
27376-991
30 cm x 3 m roll
1/pkg
Special Features
for Biodyne® Transfer
Membranes
• Do not crack, shrink, or tear when subjected
to multiple cycles of hybridization, stripping,
and reprobing
Featured Products
Biodyne A Membrane, 0.45 µm
Pall PN
VWR Cat. No.
Description
Packaging
60100
28150-282
20 x 20 cm sheets
10/pkg
60106
28150-274
30 cm x 3 m roll
1/pkg
• Intrinsically hydrophilic for easy wetting
• Superior performance with radioactive
(Biodyne B) and nonradioactive (Biodyne A)
detection systems
Biodyne B Membrane, 0.45 µm
Pall PN
VWR Cat. No.
Description
Packaging
60200
28150-284
20 x 20 cm sheets
10/pkg
60207
28150-276
30 cm x 3 m roll
1/pkg
• High sensitivity
Applications for
Biodyne Transfer
Membranes
Biodyne C Membrane, 0.45 µm
Pall PN
VWR Cat. No.
Description
Packaging
• Southern/northern blots
60314
28150-286
20 x 20 cm sheets
10/pkg
• Dot blots/reverse dot blots
60320
28150-278
30 cm x 3 m roll
1/pkg
• Macroarrays
• DNA fingerprinting
• Colony/plaque lifts
Biodyne Plus Membrane, 0.45 µm
Pall PN
VWR Cat. No.
Description
Packaging
60400
28150-288
20 x 20 cm sheets
10/pkg
60406
28150-280
30 cm x 3 m roll
1/pkg
7
Making It Simple
Contact your local VWR International representative for your Pall Life Sciences
Laboratory Filtration and Separations Catalog.
Pall Life Sciences
600 South Wagner Road
Ann Arbor, MI 48103-9019 USA
800.521.1520 toll free in USA
734.665.0651 phone
734.913.6114 fax
Visit us on the Web at www.pall.com/lab
E-mail us at Lab@pall.com
© 2004, Pall Corporation. Pall,
, Acrodisc, Supor, VacuCap, Nanosep, Omega, BioTrace, and Biodyne are trademarks
of Pall Corporation. ® Indicates a registered trademark in the USA.
is a service mark
of Pall Corporation.
12/04, 5K GN04.1078
PN33288