www.pall.com/lab Achieve Optimal Results In Analytical Chemistry Sample Preparation and Mobile Phase Filtration Proven Performance for Confident Results Whether you are processing single samples or thousands of samples at a time, you can count on our unfailing quality. Over thirty years ago, Pall Life Sciences revolutionized sample preparation for analytical chemists with the development of the Acrodisc® syringe filter. Today, we partner with scientists around the world to develop distinctive filtration and separation products for a wide range of processing volumes. We are constantly looking for new ways to expand our product offering to meet your needs and ease your application challenges. Our product innovations are the result of understanding your applications and valuing the amazing contributions your work can make to the quality of all our lives. Whether you are pursuing goals in life science, pharmaceutical methods, research and development, quality control, or specialty environmental applications, we commit to not only deliver a product that works, but to look beyond what simply works and deliver a product that truly makes a difference. 2 Focused on producing products that give you better results for high throughput sample preparation. Pall Life Sciences is one of the few companies to offer a variety of products made from the same materials of construction, allowing for single- or multiple-sample processing of your techniques. We bring together membranes with superior performance, outstanding housing materials, and devices designed to maximize processing accuracy and speed. Single-Sample Processing Select the device configuration most appropriate for your Acrodisc PSF Syringe Filters (Automated Applications) application, then select one of our patented membranes with the characteristics you need most... and see what a difference Pall Life Sciences can make. Multi-Sample Processing AcroPrep™ Advance and AcroPrep Filter Plates See pages 6-7 for more information. Perfect for smaller sample sizes in combinatorial chemistry cleavage applications Makes concentration and final filtration for SPE faster Great for numerous samples with HPLC sample preparation/clarification Perform quick and accurate metabolic studies AcroPrep 24 Filtration System See pages 8-9 for more information. Designed to work specifically with Waters Alliance◆ HPLC system Improves productivity in methods development See pages 10-12 for more information. Acrodisc PSF syringe filters are the only syringe filters to receive SOTAX◆ and Zymark◆ Automation Certified guarantees Smooth operation and worry-free performance 24 hours a day For unattended processing of large numbers of samples with robotic workstations Traditional Acrodisc Syringe Filters (Manual Applications) See pages 13-15 for more information. Broad line of syringe filters for all your HPLC/UHPLC needs Wide range of device sizes for minimal sample hold-up and easy dispensing into autosampler vials GHP Nanosep® MF Centrifugal Devices See page 16 for more information. For applications requiring maximum filtrate recovery from limited sample volumes Consistent, reproducible results for quality control Speeds up sample clarification www.pall.com/lab 3 Filter Media Selection is as Easy as 1-2-3 Pall’s broad selection of membrane chemistries ensures optimal performance for your application. Pall’s superior media separation technology ensures HPLC Certified for Low Extractables protection of your analytical instruments and supports the Pall Life Sciences HPLC certification ensures that analytical integrity of your analytical results. Pall’s capabilities ensure: results will not be compromised by extractable filter materials. Accurate pore size ratings for better chromatography and instrument/column protection (see page 11) Uniform membrane materials (lot-to-lot) to provide consistent analytical results Low extractable materials for less chromatographic interference Materials of construction selected specifically for analytical analysis with lower extractables and lower API adsorption 1 Consider Chemical Compatibility Chemical compatibility is defined as the ability of a filter material to resist select chemicals so that the pore structure is not adversely affected by chemical exposure, and the filter material does not shed particles or fibers to add extractables. For example, nylon is not recommended for strong acidic solutions due to incompatibility. The chemical compatibility chart on page 19 will help you make the right choice. Temperature, time, concentration, applied pressure, and length of exposure also affect compatibility. Acrodisc® and Acrodisc PSF syringe filters are randomly chosen and tested for compatibility with common solvents using UV detection. Does not include HT Tuffryn®, Versapor®, or Supor® membrane versions. IC Certified for Low Levels of Inorganic Extractables Pall Life Sciences certified Ion Chromatography (IC) Acrodisc and Acrodisc PSF syringe filters have been tested using a highly sensitive IC protocol to monitor ionic extractables. Actual background levels of filter extractables are typically less than 20 ppb for chloride, 6 ppb for nitrate, 1 ppb for phosphate, and 10 ppb for sulfate. Binding Membranes may chemically interact with the sample through electrostatic, ionic, covalent, hydrogen bonding, or other interactions. Binding can be a desirable or undesirable characteristic depending on the requirements of the application. Choose GHP membrane for applications requiring low binding. Reference Material Extractable Materials Also available online in our Literature Library Contaminants that elute from the filter media are best at www.pall.com/lab. prevented by the membrane manufacturer. Pall Life Sciences specifically selects the highest grade of materials and performs rigorous extraction methods on our membrane products to reduce the occurrence of undesired artifacts. Choosing membranes that are compatible with your fluids and experimental conditions will reduce or eliminate extractables. 4 To verify low levels of extractables, samples of HPLC Technical Guide, Use of Acrodisc Syringe Filters for Analytical Sample Preparation; Including HPLC and Dissolution Testing (PN 33454) Non-Aggressive Organic Aggressive Organic General Aqueous Proteinaceous Media Selection GH Polypro (GHP) Membrane – GHP is an all-purpose, hydrophilic membrane for aqueous, acidic, basic, non-aggressive organic, and aggressive organic solutions. GHP membrane offers low protein binding and low levels of UV-absorbing extractables. PTFE Membrane – Exceptional chemical and temperature compatibility make this membrane ideal for harsh chemicals that destroy other membrane materials. Nylon Membrane – This versatile filter for both aqueous and solvent-based sample filtration exhibits excellent chemical compatibility with esters, bases, and alcohols. Not recommended for acids > 1 N or halogenated solvents. Volume to be Filtered Filter Type Typical Hold-Up Volume < 500 µL Nanosep® MF Device < 2 µL < 900 µL AcroPrep™ 96 1 mL Filter Plate < 18 µL/well < 2 mL 4 mm Acrodisc Syringe Filter < 10 µL < 2 mL AcroPrep 24 Filter Plate < 50 µL/well < 10 mL 13 mm Acrodisc Syringe Filter (Minispike) < 14 µL < 10 mL 13 mm Acrodisc Syringe Filter < 30 µL < 100 mL 25 mm Acrodisc Syringe Filter < 150 µL < 125 mL 25 mm Acrodisc PSF Syringe Filter < 200 µL ** PVDF Membrane – Compatible with many organic and non-organic solvents making it ideal for a wide range of applications. Not recommended for acetone, DMF, DMSO, or bases > 6 N. Glass Fiber Media – Maximize throughput for extremely particulate-laden samples. Supor (PES) Membrane for Ion Chromatography (IC) – Optimized to provide the most consistent results when analyzing ionic species, even for the most sensitive analyses. Certified for low levels of ionic extractables. Hydrophilic Membranes for General Aqueous Samples – Widely used in dissolution testing, choose from a variety of pore sizes and membrane types for prefiltration and clarification including Supor, HT Tuffryn‚ and Versapor membranes. 3 Choose the Right Pore Size Pore size is best selected by considering the instrumentation used for analysis. UV/V spectrophotometers may only require 1 µm filtration; HPLC analysis may require 0.45 µm filtration; and UHPLC will require 0.2 µm filtration due to the size of the column packing, beads, and internal frits. The Recommended Suitable Not Recommended **Dependent on protein type and concentration. filter material’s pore size is determined by the diameter of the smallest particle that is to be retained with a defined, 2 Consider Effective Filtration Area (EFA) The particulate contained within a fluid affects the life of a filter. As particles are removed from a filter, they block pores and reduce the useable portion of the filter. Fluids with particulate loads will plug a filter more quickly than “clean” fluids. Increasing the EFA can lengthen the life of a filter. The following table outlines general guidelines for the most appropriate filter size for different volumes of liquid. high degree of efficiency. For standard liquid chromatography systems using columns with 5 µm or larger packings, the filtration industry standard is 0.45 µm for syringe filters and mobile phase membranes. For columns with packings smaller than 5 µm, UHPLC, microbore columns, or when concerned about microbial growth, a 0.2 µm filter is recommended. To clarify samples or when processing difficult-to-filter solutions, 1 to 5 µm pore sizes or glass fiber filters are suggested. Prefilters generally precede smaller pore size final filters and allow the user to process larger fluid volumes before the filter plugs. www.pall.com/lab 5 AcroPrep ™ Advance and AcroPrep Filter Plates for Sample Clean-Up in Analytical Applications As samples get smaller and more numerous, you need Serialized Barcode new methods for high throughput sample preparation. Serialized barcode label allows for the use of automated We are ready to help with high throughput screening, tracking systems. combinatorial chemistry, and sample clean-up in analytical or solid-phase extraction applications. Performance Observed Crosstalk in AcroPrep Filter Plate = 0% Counts Per Second (CPS) 1.E+07 1.E+06 1.E+05 5.2 X 104 1.E+04 1.E+03 1.E+02 0 50 100 150 200 Well Number Fluorescein dye (green) (200 µL of a 2 µg/mL H2O stock) was added to wells of AcroPrep plates with GHP membrane (5 plates total) in a checkerboard pattern. Alternate wells were filled with 200 µL of water. The fluid was evacuated from each filter plate using vacuum filtration at 30 cm Hg (12 in. Hg) for 15 seconds and the filter plate read in a PerkinElmer, Wallac VICTOR◆ 1420 Multilabel Counter. Wells filled with water (orange), which show a CPS reading above the dashed line (5.2 x 104 CPS, which is = “Average” Background + 5% Signal) in the graph, constitute a crosstalk event. Special Features Worry-Free Performance The robotic-friendly design has a rigid, single-piece construction, designed in accordance with American National Standards Institute (ANSI) specifications. Applications by Membrane Type GHP Membrane HPLC sample prep and clarification Metabolic studies No Crosstalk Membranes are sealed in separate wells using a proprietary sealing technology and extended flow directors eliminate crosstalk of filtrate. Our proprietary design also eliminates weeping for aqueous solutions and drastically reduces weeping for organic solutions. Chemically Resistant Plate Assembly Constructed from chemically-resistant, biologically-inert polypropylene. 6 Applications that use harsh organics Combinatorial chemistry library screens Solvent-based applications PTFE Membrane Molecular synthesis Drug synthesis reactions Glass Fiber Media Gross fractionation Cell-based assays Glass Fiber Over Supor® Membrane Clarification of cell lysates and tissue homogenates Preparation of proteinaceous solutions Applications that require prefiltration Mustang® Membrane Protein fractionation Protein capture Ordering Information Antibody purification AcroPrep 96-Well Filter Plates (Natural) Vacuum Manifold Designed to Perfectly Fit AcroPrep Filter Plates The multi-well plate vacuum Part Number 5052 5054 5065 Description 1 mL, 0.2 µm, GHP membrane 1 mL, 0.45 µm, GHP membrane 1 mL, 1.2 µm, Supor membrane, valve mat manifold is an anodized AcroPrep Advance 96-Well Filter Plates (Natural) aluminum manifold that has Part Number 8047 8048 8147 8148 8131 8175 been designed and optimized for vacuum filtration of Pall’s multi-well filter plates. For more information on our full line of 96and 384-well filter plates, visit www.pall.com/lab. Reference Material 8171 8172 8247 Description 350 µL, 0.2 µm PTFE membrane 350 µL, 0.45 µm PTFE membrane 1 mL, 0.2 µm PTFE membrane 1 mL, 0.45 µm PTFE membrane 1 mL, 1.0 µm, glass fiber media 1 mL, 3.0 µm glass fiber media/0.2 µm Supor membrane 1 mL, 0.8 µm, Mustang Q membrane 1 mL, 0.8 µm, Mustang S membrane 2 mL, 0.2 µm PTFE membrane Also available online in our Literature Library Accessories and Replacement Parts at www.pall.com/lab. Part Number 5017 5225 5230 5231 8001 Product Data, AcroPrep Advance Filter Plates, PN 33577 Product Data, AcroPrep 96 Filter Plates Scientific and Technical Report, Automated Purification of Combinatorial Libraries Using AcroPrep 96 Filter Plate with GHP Membrane, PN 33245 Description Multi-well plate vacuum manifold Adapter collar for centrifugation Cap mat for incubation AcroPrep multi-well plate lids AcroPrep Advance multi-well plate lids Pkg 5/pkg 5/pkg 5/pkg Pkg 10/pkg 10/pkg 5/pkg 5/pkg 5/pkg 5/pkg 5/pkg 5/pkg 5/pkg Pkg 1/pkg 2/pkg 5/pkg 10/pkg 10/pkg www.pall.com/lab 7 AcroPrep ™ 24 Filtration System Simultaneously Filters Up to 24 Samples in Seconds This system eliminates the time consuming one-at-a-time Special Features syringe filter process. The AcroPrep 24 system filters Save Time and Materials directly into vials loaded in a Waters Alliance◆ 2690 sample Save up to 20 minutes of labor time per carousel over the carousel.* AcroPrep 24 filter plates are available with the conventional syringe filter technique. same HPLC certified membranes as our trusted Acrodisc® PSF syringe filters, eliminating the need to validate the AcroPrep 24 filter plates. Syringe Filter Technique 24 disposable syringes + 24 syringe filters 25 minutes AcroPrep 24 Filtration System 24 disposable pipette tips + 1 AcroPrep 24 filter plate 5 minutes Easy to Use No need to use cumbersome, individually wrapped disposable syringes. Use disposable pipette tips which are less costly, easier to use, and require less bench space. Compatible With All 12 x 32 mm Vial Systems Can be used with any HPLC autosampler that houses 12 x 32 mm vials. Simply transfer your filtered samples from the Waters Alliance sample carousel to save time and labor. No Cross-Contamination Each 1.9 mL well is individually sealed, ensuring your sample will accurately dispense into the Waters Alliance sample carousel with no cross-contamination or splashing. HPLC Certified Ensures that analytical results will not be compromised by extractable filter materials. Simple Validation Materials of construction are identical to Pall’s Acrodisc PSF syringe filters. If you are currently using Acrodisc PSF syringe filters, this will reduce the requirements for validation of the AcroPrep 24 filter plates. *The Water’s Alliance 2690 sample carousel kit (PN WAT270328) can be ordered directly from your local Waters sales office or contact Waters Corporation at 800-252-4752 (in the USA) or 508-478-2000. 8 Applications Methods development Research and development Quality control Combinatorial chemistry synthesis Reference Material Also available online in our Literature Library at www.pall.com/lab. Product Data, AcroPrep 24 Filtration System, PN 33241 Ordering Information AcroPrep 24 Filter Plates Part Number 186000159 186000155 186000158 600000158 186000154P 186000154 186000156 186000157 Description 0.2 µm, Nylon membrane 0.45 µm, Nylon membrane 0.2 µm, GHP membrane 0.45 µm, GHP membrane 0.45 µm, GxF/GHP membrane 0.45 µm, GHP membrane 0.45 µm, PTFE membrane 0.45 µm, PVDF membrane Pkg 10/pkg 10/pkg 10/pkg 2/pkg 10/pkg 10/pkg 10/pkg 10/pkg AcroPrep 24 Filtration System Manifold Part Number 289000159 Description Filtration manifold Pkg 1/pkg Spare Parts and Accessories Part Number 700000231 700000232 700000233 700000234 Description Glass housing for filtration manifold O-ring kit Rubber feet Manifold alignment post Pkg 1/pkg 3/pkg 3/pkg 3/pkg www.pall.com/lab 9 Three Powerful HPLC Sample Prep Benefits in One Acrodisc ® PSF Syringe Filter 1 Automation Certification for Smooth Operation and Worry-Free Performance 24 Hours a Day Pall Life Sciences has specifically designed and certified our Acrodisc PSF syringe filters to be fully compatible and reliable for use with automated equipment. The following special features make our syringe filters reliable for worry-free performance 24 hours a day: Smooth Filter-to-Filter Release The Acrodisc PSF syringe filter has been designed so the filters cannot be forced too tightly together AND or to the automation components, allowing for a AUTOMATION CERTIFIED ® smooth release, while still meeting ANSI/ISO filter luer standards. Consistent Turret Advancement The filter’s quick-releasing luers Smooth filter-to-filter release separate easily, and the slightly rounded upper Consistent turret advancement shoulder allows the filter to consistently slide under Exceptional housing strength the wedge. This results in trouble-free separation from the Strict “outside filter geometry” filter stack and smooth filter advancement along the Acrodisc PSF syringe filters from Pall Life Sciences are the only syringe filters to receive the SOTAX◆ and workstation’s track. Zymark◆ Automation Certified guarantee. This certification is only granted to syringe filters that meet the stringent requirements for automated dispensing and robotic handling. Pall works in close partnership with the SOTAX AG manufacturing and engineering teams to ensure proper fit, function, and compatibility with SOTAX and Zymark workstations. Exceptional Housing Strength The specially designed support ribbing, thick device walls, and proprietary housing weld ensure a robust seal, as well as a filter housing that can withstand excessive force both internally and externally. Strict "Outside Filter Geometry" Pall’s Acrodisc PSF syringe filters work best with SOTAX AG’s Zymark TPW◆, APW◆, and MultiDose◆ workstations. Pall’s standard Acrodisc syringe filters work best with SOTAX’s AT-70 SMART◆, CTS, and FS workstations. These robotic workstations are all uniquely designed to work with SOTAX and Zymark Automation Certified filters for proper operation. 10 Special Features Strict dimensional specifications in height and width ensure proper alignment and smooth operation. 2 3 Best Protection for HPLC Columns—Extending Column Life as Much as 46 Times GxF Multi-Layered Prefilter Provides Maximum Throughput The Acrodisc PSF syringe filter has a serial glass fiber Plugging is the most frequently encountered cause of High prefilter to allow for two to four times the throughput and Performance Liquid Chromatography (HPLC) column failure faster flow rates than standard glass fiber prefilter devices. by analytical chemists and analysts. Injection of samples The multi-layered prefilter traps particulate rated from containing particulates will eventually block the column > 40 to 1 µm. inlet, cause high column back-pressure, and shorten the normal lifetime of the column. Operations of pump components, injectors, and detectors are expected to be less troublesome when fluids are filtered. Pall Acrodisc Special rib design and proprietary weld ensure robust seal under high operating pressures. Ample headspace for filter cake to build for optimal throughput. PSF syringe filters with GHP (hydrophilic polypropylene) membrane offer the most efficient removal of particulate and prolong the life of HPLC system components. Effects of Filters on HPLC Column Life Free-floating prefilter prevents premature clogging. Column Back-Pressure, psig 4000 Unfiltered Competitor Filters Competitor Filters Pall Acrodisc PSF Filters 3500 3000 2500 2000 Multi-layered prefilter traps heterogeneous particulate throughout the matrix and on the surface, extending filter life. 1500 Heat sealed membrane ensures particulate retention with no chance of breakthrough. 1000 Applications 500 0 0 200 400 600 Number of Injections 800 1000 Among 0.45 µm rated syringe filters from three manufacturers, the Pall Acrodisc PSF syringe filters with GHP membrane have the highest average retention efficiency (94.9%) of 0.45 µm latex spheres, as well as greater lot-to-lot consistency. The data demonstrates “equivalent” filters from various manufacturers with the same removal rating differ in capabilities. Using Acrodisc PSF syringe filters with GHP membrane extended the column life 46 times with no significant increase in column back-pressure. For a full technical report, order “Use of Acrodisc Syringe Filters for Analytical Sample Preparation; Including HPLC and Dissolution Testing Application Guide,” PN 33454; or visit www.pall.com/lab. Sample preparation for HPLC, UHPLC, IC, and GC Dissolution testing Automated workstations such as SOTAX and Zymark Clarifying samples heavily laden with particulates – Environmental groundwater – Pulp and paper – Food and beverage Reference Material Also available online in our Literature Library at www.pall.com/lab. Product Data, Acrodisc PSF Syringe Filter Technical Guide, Use of Acrodisc Syringe Filters for Analytical Sample Preparation; Including HPLC and Dissolution Testing, PN 33454 www.pall.com/lab 11 Ordering Information Acrodisc® PSF Syringe Filters With GHP Membrane, 25 mm Acrodisc PSF Syringe Filters With PVDF Membrane, 25 mm Part Number AP-4364 AP-4564 AP-4566 AP-4357 AP-4560 AP-4562 AP-4305 AP-4307 AP-4306 AP-4557 AP-4559 AP-4558 Part Number AP-4795 AP-4796 AP-4797 AP-4519 AP-4408 AP-4500 AP-4792 AP-4793 AP-4794 AP-4309 AP-4310 AP-4308 Description 0.2 µm, AutoPack™ tubes 0.2 µm 0.2 µm 0.45 µm, AutoPack tubes 0.45 µm 0.45 µm GxF/0.2 µm, AutoPack tubes GxF/0.2 µm GxF/0.2 µm GxF/0.45 µm, AutoPack tubes GxF/0.45 µm GxF/0.45 µm Pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg Pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg Acrodisc PSF Syringe Filters With PTFE Membrane, 25 mm IC Acrodisc PSF Syringe Filters With Supor ® Membrane, 25 mm Part Number AP-4520 AP-4225 AP-4521 AP-4518 AP-4219 AP-4501 AP-4789 AP-4790 AP-4791 AP-4301 AP-4303 AP-4302 Part Number AP-4801 AP-4802 AP-4803 Description 0.2 µm, AutoPack tubes 0.2 µm 0.2 µm Pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg AP-4587 AP-4585 AP-4785 0.45 µm, AutoPack tubes 0.45 µm 0.45 µm 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg Description 0.2 µm, AutoPack tubes 0.2 µm 0.2 µm 0.45 µm, AutoPack tubes 0.45 µm 0.45 µm GxF/0.2 µm, AutoPack tubes GxF/0.2 µm GxF/0.2 µm GxF/0.45 µm, AutoPack tubes GxF/0.45 µm GxF/0.45 µm Pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg Acrodisc PSF Syringe Filters With Nylon Membrane, 25 mm Part Number AP-4437 AP-4436 AP-4522 AP-4517 AP-4438 AP-4502 AP-4786 AP-4787 AP-4788 AP-4548 AP-4549 AP-4528 Description 0.2 µm, AutoPack tubes 0.2 µm 0.2 µm 0.45 µm, AutoPack tubes 0.45 µm 0.45 µm GxF/0.2 µm, AutoPack tubes GxF/0.2 µm GxF/0.2 µm GxF/0.45 µm, AutoPack tubes GxF/0.45 µm GxF/0.45 µm Pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg Acrodisc PSF Syringe Filters With Glass Fiber, 25 mm Part Number AP-4527 AP-4523 AP-4529 12 Description 0.2 µm, AutoPack tubes 0.2 µm 0.2 µm 0.45 µm, AutoPack tubes 0.45 µm 0.45 µm GxF/0.2 µm, AutoPack tubes GxF/0.2 µm GxF/0.2 µm GxF/0.45 µm, AutoPack tubes GxF/0.45 µm GxF/0.45 µm Description GxF/Glass, AutoPack tubes GxF/Glass GxF/Glass Pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg Acrodisc PSF Syringe Filters With Versapor ® Membrane, 25 mm Part Number AP-4190 AP-4189 AP-4568 AP-4000 AP-4001 AP-4002 Description 0.8 µm, AutoPack tubes 0.8 µm 0.8 µm 10 µm, AutoPack tubes 10 µm 10 µm Pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg Acrodisc PSF Syringe Filters With HT Tuffryn® Membrane, 25 mm Part Number AP-4498 AP-4497 AP-4784 Description 0.45 µm, AutoPack tubes 0.45 µm 0.45 µm Pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg Acrodisc PSF Syringe Filters With Supor® Membrane, 25 mm Part Number AP-4798 AP-4799 AP-4800 AP-4424 AP-4425 AP-4426 Description GxF/0.2 µm, AutoPack Tubes GxF/0.2 µm GxF/0.2 µm GxF/0.45 µm, AutoPack Tubes GxF/0.45 µm GxF/0.45 µm Pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg Acrodisc Syringe Filters Ensure Complete Sample Filtration With Minimal Hold-Up Volumes Analytical sample prep syringe filters are HPLC certified Membrane Color Code for low levels of UV-absorbing extractables. We guarantee absolute satisfaction. GH Polypro PTFE Nylon Glass Fiber pore size on each filter. This makes them ideal for ISO labs. PES < 150 mL (3.9 cm2) < 150 mL (3.9 cm2) < 150 mL (3.9 cm2) < 2 mL (0.3 cm2) < 10 mL (1.0 cm2) < 10 mL (1.0 cm2) < 10 mL (1.0 cm2) < 100 mL (2.8 cm2) < 100 mL (EFA varies by device size) < 200 mL (7.5 cm2) GH P Relative Throughput (EFA) Filter Formats Acrodisc PSF Syringe Filters 25 mm Acrodisc PSF Syringe Filter: designed and automation certified to address the specific requirements of automated workstations. 25 mm Acrodisc PSF GxF Syringe Filter: multi-layered glass prefilter to maximize throughput and meet the requirements of automated workstations. 25 mm Acrodisc PSF IC Syringe Filter: optimized to provide consistent results when analyzing ionic species. (P (P Hy olyp d r PT rophilopyle ic) ne FE , (H yd ro ph Ny ob lon ic) (H yd ro ph PV ilic DF ) (H yd r o Ve ph ilic rsa ) p hil have color-coded printing indicating membrane type and e, (P Hy oly dr su op lfo hil ne ic) , configurations to fit all applications. Acrodisc syringe filters PVDF ic) HT Hydrolyet Tu oph hers i u ffr yn lic) lfon wide range of membrane types, sizes, and packaging or (A Gla Hy cry dr lic ss o Fib philicCopo ) lym er er, Su (H yd po ro r p Traditional Acrodisc syringe filters are available in a AND AUTOMATION CERTIFIED ® AND AUTOMATION CERTIFIED ® AND AUTOMATION CERTIFIED ® Acrodisc Syringe Filters 4 mm Acrodisc Syringe Filter: ideal for filtration when sample size is minimal. 13 mm Acrodisc Syringe Filter: multiple filter selection for both aqueous and solvent-based sample filtration. 13 mm Acrodisc Syringe Filter with Minispike: minispike configuration offers lower hold-up volume and easy filtration into autosampler vials. 13 mm IC Acrodisc Syringe Filter: convenient size for small sample volumes of ionic solutions. 25 mm Acrodisc Syringe Filter: multiple membrane selections for compatibility with aqueous and organic solvents. Non-Sterile Acrodisc Syringe Filter (modified acrylic housings): for general aqueous samples, prefiltration, and clarification. Not HPLC certified. 37 mm GF Acrodisc Syringe Filter: additional filtration area for very dirty samples. Not HPLC certified. Note: All HPLC- and IC-certified Acrodisc syringe filters are made with polypropylene housings and male luer outlets; the 13 mm is also available with minispike outlets. www.pall.com/lab 13 Applications Sample prep for UHPLC Sample prep for microbore columns For use when concerned with microbial growth Reference Material Also available online in our Literature Library at www.pall.com/lab. Product Data, SolVac® Filter Holder Product Data, Vacuum/Pressure Pumps Product Data, Stainless Steel Forceps High Performance UHPLC Sample Prep Filtration Provides Increased Column Life and the Best Chromatography UHPLC requires stringent sample preparation to improve chromatography and extend column life. The smaller column packing sizes in UHPLC columns require smaller pore sized Ordering Information Acrodisc Syringe Filters With GHP Membrane, 13 mm Part Number 4554 4567 4556 4563 Description 0.2 µm, minispike outlet 0.2 µm, minispike outlet 0.45 µm, minispike outlet 0.45 µm, minispike outlet sample prep filters. These 0.2 µm syringe filters and Acrodisc Syringe Filters With GHP Membrane, 25 mm membranes have been developed for UHPLC sample prep Part Number 4564 4566 4560 4562 4559 4558 and mobile phase filtration. The 0.2 µm pore size assures removal of the contaminant size most likely to plug a UHPLC column and offers better protection of your analytical results. As with other pore size Acrodisc® syringe filters, these Description 0.2 µm 0.2 µm 0.45 µm 0.45 µm GF/0.45 µm GF/0.45 µm Pkg 100/pkg, 300/cs 1000/pkg 100/pkg, 300/cs 1000/pkg Pkg 50/pkg, 200/cs 1000/pkg 50/pkg, 200/cs 1000/pkg 50/pkg, 200/cs 1000/pkg UHPLC filter membranes (GHP, nylon, PVDF, and PTFE) are HPLC certified for low levels of UV absorbing extractables. Acrodisc Syringe Filter With PTFE Membrane, 4 mm Acrodisc PSF syringe filters are available with a GxF Part Number 4472 Description 0.45 µm Pkg 250/pkg, 750/cs multi-layer prefilter which provides two to four times the throughput of standard glass fiber prefilter devices, allowing Acrodisc Syringe Filters With PTFE Membrane, 13 mm quick and easy filtration of your most difficult to filter solutions. Part Number 4552 4553 4555 4423 4542 4422 4543 Pall’s 13 mm Acrodisc syringe filter can also be purchased with a 13 mm minispike outlet for lower hold-up volume and easier filtration into autosampler vials without airlock. 14 Description 0.2 µm, minispike outlet 0.45 µm, minispike outlet 0.45 µm, minispike outlet 0.2 µm, male slip luer outlet 0.2 µm, male slip luer outlet 0.45 µm, male slip luer outlet 0.45 µm, male slip luer outlet Pkg 100/pkg, 300/cs 100/pkg, 300/cs 1000/pkg 100/pkg, 300/cs 1000/pkg 100/pkg, 300/cs 1000/pkg Acrodisc Syringe Filters With PTFE Membrane, 25 mm Part Number 4225 4521 4518 4219 4501 4226 4503 Description 0.2 µm 0.2 µm 0.45 µm, AutoPack™ tubes 0.45 µm 0.45 µm 1 µm 1 µm Acrodisc Syringe Filters With PVDF Membrane, 13 mm Pkg 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg 50/pkg, 200/cs 1000/pkg Part Number 4450 4544 4452 4545 4455 4457 Description 0.2 µm, minispike outlet 0.2 µm, minispike outlet 0.45 µm, minispike outlet 0.45 µm, minispike outlet 0.2 µm, male slip luer outlet 0.45 µm, male slip luer outlet Pkg 100/pkg, 300/cs 1000/pkg 100/pkg, 300/cs 1000/pkg 100/pkg, 300/cs 100/pkg, 300/cs Acrodisc Syringe Filters With PVDF Membrane, 25 mm Acrodisc Syringe Filter With Nylon Membrane, 4 mm Part Number 4484 Description 0.45 µm Pkg 250/pkg, 750/cs Acrodisc Syringe Filters With Nylon Membrane, 13 mm Part Number 4550 4561 4551 4546 4427 4540 4426 4541 Description 0.2 µm, minispike outlet 0.2 µm, minispike outlet 0.45 µm, minispike outlet 0.45 µm, minispike outlet 0.2 µm, male slip luer outlet 0.2 µm, male slip luer outlet 0.45 µm, male slip luer outlet 0.45 µm, male slip luer outlet Pkg 100/pkg, 300/cs 1000/pkg 100/pkg, 300/cs 1000/pkg 100/pkg, 300/cs 1000/pkg 100/pkg, 300/cs 1000/pkg Part Number 4406 4520 4519 4408 4500 Description 0.2 µm 0.2 µm 0.45 µm, AutoPack tubes 0.45 µm 0.45 µm Pkg 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg Ion Chromatography Acrodisc Syringe Filters, 13 mm Part Number 4483 4683 4485 4685 Description 0.2 µm 0.2 µm 0.45 µm 0.45 µm Pkg 100/pkg, 300/cs 1000/pkg 100/pkg,300/cs 1000/pkg Ion Chromatography (PES) Acrodisc Syringe Filters, 25 mm Acrodisc Syringe Filters With Nylon Membrane, 25 mm Part Number 4436 4522 4517 4438 4502 4548 4549 4528 Description 0.2 µm 0.2 µm 0.45 µm, AutoPack tubes 0.45 µm 0.45 µm GF/0.45 µm, AutoPack tubes GF/0.45 µm GF/0.45 µm Pkg 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg Acrodisc Syringe Filters With Glass Fiber, 25 mm Part Number 4527 4523 4529 Description 1 µm (nominal), AutoPack tubes 1 µm (nominal) 1 µm (nominal) Pkg 25/pkg, 200/cs 50/pkg, 200/cs 1000/pkg Part Number 4583 4783 4585 4785 Description 1 µm (nominal), polypropylene housing Pkg 15/pkg, 60/cs Pkg 50/pkg, 200/cs 1000/pkg 50/pkg, 200/cs 1000/pkg Acrodisc Syringe Filters With Versapor® Membrane, Non-Sterile Part Number 4473 4459 4488 4489 Description 0.45 µm, 4 mm 0.8 µm, 13 mm, polypropylene housing 1.2 µm, 25 mm, modified acrylic housing 5 µm, 25 mm, modified acrylic housing Pkg 250/pkg, 750/cs 300/cs 75/pkg, 300/cs 75/pkg, 300/cs Acrodisc Syringe Filters With Supor® Membrane, Non-Sterile Part Number 4655 4659 Acrodisc Syringe Filter With Glass Fiber, 37 mm Part Number 4524 Description 0.2 µm 0.2 µm 0.45 µm 0.45 µm 4653 4661 4660 4662 Description 0.2 µm, 32 mm, modified acrylic housing 0.8/0.2 µm, 32 mm, modified acrylic housing 0.45 µm, 32 mm, modified acrylic housing 1.2/0.45 µm, 32 mm, modified acrylic housing 1.2 µm, 32 mm, modified acrylic housing 5 µm, 32 mm, modified acrylic housing Pkg 1000/pkg 1000/pkg 1000/pkg 1000/pkg 1000/pkg 1000/pkg www.pall.com/lab 15 Nanosep® Centrifugal Devices are Ideal When Sample Recovery is a Concern 96.5% Protein Recovery GH Polypro membrane is a low protein binding membrane. It removes unwanted particulate from samples with high recovery of critical proteins. Low Extractables Our HPLC-grade centrifugal devices are certified to be low in UV extractables. Leak-Free Operation Unique sealing technology assures leak-free operation without the use of o-rings or adhesives that can add extractables. Applications Sample prep – particulate removal prior to sample analysis, HPLC grade Removal of precipitates, including metals, polymers, and crystals Removal of cells from media prior to analysis GHP Nanosep MF centrifugal devices are perfect for particulate removal prior to analytical sample analysis. The high g-force rating allows spinning at 14,000 x g, assuring rapid sample processing. Special Features Rapid Processing of Samples Centrifugal devices are simple to use and save on sample preparation time. Spin multiple samples at once. Universal Membrane Filter The GH Polypro hydrophilic polypropylene membrane is ideal for aqueous solutions and offers maximum chemical compatibility for aggressive solvents. 16 Applications requiring maximum filtrate (or retentate) recovery from limited sample volumes Ordering Information GHP Nanosep MF Centrifugal Devices Part Number ODGHPC34 ODGHPC35 Description 0.45 µm, clear 0.45 µm, clear Pkg 100/pkg 500/pkg Simplify Clean-Up and Degassing of Mobile Phase Solvents With a SolVac ® Filter Holder Special Features Versatile Design Fits Most HPLC Bottles, Flasks, and Containers Eliminates the added step of transferring mobile phase solvent from flask to reservoir, which can cause contamination. Draws Directly From HPLC Solvent Bottles Less likely to spill aggressive solvents than glass funnels or disposable cups. Durable Plastic Construction Resists breaking, unlike glass funnels, assemblies, or pick-up adapters. Leak-Proof Patented Magnetic Seal Eliminates membrane shifting or tearing which can occur with aluminum clamps or threaded holders. Ordering Information SolVac Filter Holder for Mobile Phase Part Number 4020 How to Use 1. Place base on the receiving vessel.* Place the membrane on the clean, dry filter support. 2. Attach inlet feedline tubing to the upper housing. Place upper housing onto housing base to securely seal the membrane. 3. Attach vacuum tubing from vacuum source to the vacuum port adapter on the housing base. 4. Place feedline tubing into solvent to be filtered. 5. Apply vacuum to pull liquid through the filter. 4022 4023 4025 4026 4028 Description SolVac holder with 61 cm (2 ft.) feedline tubing, thumb clamp, sinker, vacuum port adapter, 2 membrane seal gaskets, and 2 seal gaskets 122 cm (4 ft.) replacement feedline tubing Replacement seal gaskets Membrane seal gasket kit Sinker replacement kit Clamp replacement kit Pkg 1/pkg 1/pkg 10/pkg 10/pkg 2/pkg 2/pkg Mobile Phase Membranes Part Number 66557 66628 66548 66625 66143 66630 66149 66631 66477 66480 66602 66608 Description 0.2 µm, 47 mm GH Polypro (PP) 0.2 µm, 50 mm GH Polypro (PP) 0.45 µm, 47 mm GH Polypro (PP) 0.45 µm, 50 mm GH Polypro (PP) 0.2 µm, 47 mm TF (PTFE) 0.2 µm, 50 mm TF (PTFE) 0.45 µm, 47 mm TF (PTFE) 0.45 µm, 50 mm TF (PTFE) 0.2 µm, 47 mm FP Vericel™ (PVDF) 0.45 µm, 47 mm FP Vericel (PVDF) 0.2 µm, 47 mm Nylaflo™ (Nylon) 0.45 µm, 47 mm Nylaflo (Nylon) Pkg 100/pkg 100/pkg 100/pkg 100/pkg 100/pkg 100/pkg 100/pkg 100/pkg 100/pkg 100/pkg 100/pkg 100/pkg *Always use a safety-coated receiving vessel that is less than 4 L and rated for vacuum applications. Failure to do so may result in implosion of the receiving vessel and potential injury to the user. www.pall.com/lab 17 47 mm Glass Filter Funnels for Vacuum Filtration of Liquids and Degassing of HPLC Solvents and Mobile Phases Mobile Phase Membranes Pall membrane disc filters provide purification and degassing of mobile phase solvents used in liquid chromatography applications. The membranes are identical in composition and quality to those used in our HPLC-certified Acrodisc® syringe filters. HPLC Mobile Phase Filtration Membranes Membrane TF (PTFE) Mobile Phase Application Recommended for use with all lower surface tension liquids GHP (Polypropylene) Recommended for use with organics and aqueous liquids Nylaflo™ (Nylon) Not recommended for use with certain acidic solutions FP Vericel™ (PVDF) Not recommended for use with certain basic solutions Supor® (PES) Not recommended for use with certain ketones Ordering Information 47 mm Glass Filter Funnels Special Features Part Number 4011 Resistant to Aggressive Solvents Funnel is made of 100% borosilicate glass, assuring 4012 resistance to even the most aggressive solvents. 4013 Description Glass filter funnel with No. 8 stopper support base (300 mL funnel, no flask) Glass filter funnel with sidearm support assembly and flask (1 L funnel with 4 L flask) Glass filter funnel with sidearm support assembly and flask (300 mL funnel with 1 L flask) Pkg 1/pkg 1/pkg 1/pkg Filter an Entire Liter at Once One-liter 47 mm glass funnel/support assembly permits filtration of an entire liter at once. Unique Design Support assembly’s unique base design with integral Replacement Parts 47 mm Glass Filter Funnel (for PN 4011) Part Number 4014 4019 81595 Description Upper glass funnel, 300 mL Fritted glass support base/No. 8 silicone stopper Aluminum clamp, anodized Pkg 1/pkg 1/pkg 1/pkg vacuum connection prevents contamination of the vacuum line with filtrate. Easy to Read One-liter glass funnel is graduated from 300 to 1000 mL in 50 mL increments. 300 mL glass funnel is graduated from 100 to 250 mL in 25 mL increments. Stepped stem fits into standard one-hole stoppers (9 mm). 18 47 mm Glass Funnel/Support Assembly (for PN 4012, 4013) Part Number 4015 4018 4016 4017 81595 Description Glass funnel, 1 L Glass flask, 1L Glass flask, 4 L Fritted glass support base with sidearm Aluminum clamp, anodized Pkg 1/pkg 1/pkg 1/pkg 1/pkg 1/pkg Eth yl a cet ate Eth yl e the r Fre on TF Hyd roc hlor ic R R R R R R R R R R R R R R R R R R L R R R R R* R R* R* R R R* R* R* R R R R R R R R R L R R* R* R* R* R R L R L R R R R R N N R R* R R R R R R N N R N R N R R R R* R* R R* R R N R R R R* R* R N R R R N • N N R N R L R L R N N N R R R R R R R R R R R R R R R R R N N N N R N N R L N L* N N N N N N N N N R* • L N N N N N N N N R* N L* R R N R Ace ton itril e Ace tic acid , gl acia n-B l uta nol Chl oro form Dio xan e R Ace ton e Dim eth yl f orm am Dim ide eth yl s ulfo xide Eth ano l acid Hex (1N ) ane , dr y Me tha nol Me thy lene chlo ride Me thy l et hyl ket N-M one eth ylpy rrol idon Isop e rop ano l Sod ium hyd rox Tetr ide ahy (3N dro ) fura n Tetr ahy dro fura n/w Tolu ate r (5 ene 0/5 0) Wa ter Chemical Compatibility Guide Devices with GH Polypro (GHP) membrane (hydrophilic polypropylene) R R R R R* R R 13 & 25 mm, AcroPrep™ R* R R R R R • L R R* R R N • R* R L R R R* R R R N • R R R R L L • R R N N R R N • N R N N N L R N • N R N N N L R N • N R Devices with PTFE membrane 4 mm Devices with PVDF membrane N Devices with Nylon membrane R* Devices with Supor PES membrane Devices with Glass Fiber Devices with Versapor® membrane (modified acrylic housing) N N N R Devices with Supor membrane (modified acrylic housing) N L N R Devices with HT Tuffryn® membrane (modified acrylic housing) N N N R R R N R R R R R Disc Filters GH Polypro R R FP Vericel (PVDF) N R Nylaflo (Nylon) R R TF (PTFE) R R R R R R R R R R R R N N R R R R R R R R R R R R R R R R R R R R R R R R R R R R R R R R R R N N R N N • R R R R R R R R L R R R R R R R R R R R R R R R * UV absorbance was set at 254 nm. Test Methods The data presented in this chart is a compilation of testing by Pall Corporation with certain chemicals, manufacturer’s data, or compatibility recommendations from the Compass Corrosion Guide, by Kenneth M. Pruett. This data is intended to provide expected results when filtration devices are exposed to chemicals under static conditions for 48 hours at 25 °C (77 °F), unless otherwise noted. This chart is intended only as a guide. Accuracy cannot be guaranteed. Users should verify chemical compatibility with a specific filter under actual use conditions. Chemical compatibility with a specific filter, under actual use conditions, is affected by many variables including temperature, pressure, concentration, and purity. Various chemical combinations prevent complete accuracy. R = Resistant No significant change was observed in flow rate or bubble point of the membrane. L = Limited Resistance Moderate changes in physical properties or dimension of the membrane were observed. The filter may be suitable for short term, non-critical use at room temperature. N = Not Resistant The membrane is basically unstable. In most cases, extensive shrinkage or swelling occurs. The filter may gradually weaken or partially dissolve after extended exposure. • = Insufficient Data Information not available. Trial testing is recommended. www.pall.com/lab 19 Visit us on the Web at www.pall.com/lab E-mail us at LabSupport@pall.com Pall Life Sciences 600 South Wagner Road Ann Arbor, MI 48103-9019 USA 1.800.521.1520 USA and Canada (+)800.PALL.LIFE Outside USA and Canada 1.734.665.0651 phone 1.734.913.6114 fax International Offices Pall Corporation has offices and plants throughout the world in: Argentina, Australia, Austria, Belgium, Brazil, Canada, China, France, Germany, India, Indonesia, Ireland, Italy, Japan, Korea, Malaysia, New Zealand, Norway, Philippines, Poland, Russia, Singapore, South Africa, Spain, Sweden, Switzerland, Taiwan, Thailand, United Kingdom, and Vietnam. Distributors in all major industrial areas of the world. To locate the Pall office or distributor nearest you, visit www.pall.com/contact. The information provided in this literature was reviewed for accuracy at the time of publication. Product data may be subject to change without notice. For current information consult your local Pall distributor or contact Pall directly. © 2011, Pall Corporation. Pall, , Acrodisc, AcroPrep, AutoPack, Mustang, Nanosep, Nylaflo, Supor, SolVac, Tuffryn, Vericel, and Versapor are trademarks of Pall Corporation. ® indicates a trademark registered in the USA. Filtration.Separation.Solution. is a service mark of Pall Corporation. ◆Alliance is a trademark of Waters Corporation. VICTOR is a trademark of PerkinElmer, Inc. APW, MultiDose, SMART, SOTAX, TPW, and Zymark are trademarks of SOTAX Ag. 4/11, 5k, GN10.4380 PN33584