Product Data
GHP Nanosep® MF Centrifugal
Device, HPLC Grade
Ideal for particulate removal prior to
sample analysis (HPLC, IC, or GC)
• Rapid processing of small-volume samples.
Centrifugal devices are simple to use and save
on sample preparation time.
• Universal membrane filter. The GH Polypro
hydrophilic polypropylene membrane is ideal for
aqueous solutions and offers maximum chemical
compatibility for aggressive solvents.
• 96.5% protein recovery. GH Polypro membrane
is a low protein binding membrane. It removes
unwanted particulate from samples with high
recovery of critical proteins.
• Low extractables. Our HPLC-grade centrifugal
devices are certified to be low in UV extractables.
• Low hold-up volume (< 2 µL) makes these
devices ideal for expensive samples.
• High g-force ratings. Can be spun at 14,000 x g
assuring rapid sample processing.
• Minimizes membrane clogging. G-force
impacts the membrane at an angle, sweeping
accumulated materials from the surface.
• Leak-free operation. Unique sealing technology
assures leak-free operation without the use of
O-rings or adhesives that can add extractables.
Applications
Centrifugal devices are very simple to use. The sample
to be processed is pipetted into the upper reservoir of
the device. The device is placed into the appropriate
centrifuge (taking care that the rotor is balanced) and
spun. Filtrate can be recovered by removing the lower
reservoir of the device and using a pipette or pipette tip
to remove the liquid. Retentate can be recovered from
the GHP Nanosep MF centrifugal device simply by
pipetting it from the upper reservoir.
• Sample Prep – particulate removal prior to sample
analysis (HPLC, IC, or GC)
• Removal of precipitates (metals, polymers,
or crystals)
• Isolation of DNA from agarose gel slices
• Removal of cells from media prior to analysis
• Applications requiring maximum filtrate recovery from
limited sample volumes
Specifications
Materials of Construction
Upper Housing, Lower Reservoir: Polypropylene
Membrane: GH Polypro
(hydrophilic polypropylene)
Capacities
Sample Volume: 50 - 500 µL
Filtrate Receiver Volume: 500 µL
Hold-up Volume (membrane/support): < 2 µL
Pore Size
0.45 µm
Operating Temperature Range
0 - 40 °C (32 - 104 °F)
Effective Filtration Area
0.28 cm2
Maximum Centrifugal Force
14,000 x g
Dimensions
Overall Length (fully assembled
with cap): 4.5 cm (1.8 in.)
Centrifuge
A rotor is required that accepts
1.5 mL tubes
Performance
Low Protein Binding
The GHP Nanosep® MF centrifugal device contains low
protein binding GH Polypro membrane. In analysis of
proteinaceous biological samples, nonspecific
adsorption of sample to the filtration device is a concern.
Low protein adsorption is especially important when
small volumes of dilute protein solutions are being
filtered. Greater than 95% recovery of proteins from
GHP Nanosep MF devices is typical.
Chemical Compatibilities
Acetic acid, glacial
Acetone
Dimethyl formamide
Dimethyl sulfoxide
Ethanol
Hydrochloric acid (1N)
Isopropanol
Methanol
Methyl ethyl ketone
Methyl isobutyl ketone
R = Resistant
GHP
R
R
R
R
R
R
R
R
R
R
Chemically Resistant
With a base membrane of highly resistant hydrophilic
polypropylene, GH Polypro membrane is the only
membrane for all of your analytical filtration needs.
Nylon
NR
R
R
R
R
NR
R
R
R
R
PVDF
R
NR
NR
NR
R
R
R
R
NR
NR
NR = Not Resistant
Test Methods: The data presented in this chart is a compilation of testing by Pall with certain chemicals, manufacturer’s data, or compatibility recommendations
from the Compass Corrosion Guide, by Kenneth M. Pruett. This data is intended to provide expected results when filtration devices are exposed to chemicals
under static conditions for 48 hours at 25 °C, unless otherwise noted. This chart is intended only as a guide. Accuracy cannot be guaranteed. Users should
verify chemical compatibility with a specific filter under actual use conditions. Chemical compatibility with a specific filter under actual use conditions is
affected by many variables, including temperature, pressure, concentration, purity, and various chemical combinations which prevent complete accuracy.
Ordering Information
Product No.
Description
ODGHPC34
0.45 µm, GHP
100/pkg
ODGHPC35
0.45 µm, GHP
500/cs
Pall Life Sciences
600 South Wagner Road
Ann Arbor, MI 48103-9019 USA
800.521.1520 toll free in USA
734.665.0651 phone
734.913.6114 fax
Packaging
Australia – Lane Cove, NSW
Tel: 02 9428-2333
1800 635-082 (in Australia)
Fax: 02 9428-5610
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Tel: 043-1-49 192-0
Fax: 0043-1-49 192-400
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Tel: 905-542-0330
800-263-5910 (in Canada)
Fax: 905-542-0331
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Tel: 514-332-7255
800-435-6268 (in Canada)
Fax: 514-332-0996
800-808-6268 (in Canada)
China – P. R., Beijing
Tel: 86-10-8458 4010
Fax: 86-10-8458 4001
France – St. Germain-en-Laye
Tel: 01 30 61 39 92
Fax: 01 30 61 58 01
Lab-FR@pall.com
Germany – Dreieich
Tel: 06103-307 333
Fax: 06103-307 399
Lab-DE@pall.com
India – Mumbai
Tel: 91-22-5956050
Fax: 91-22-5956051
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Tel: 02-47796-1
Fax: 02-47796-394
or 02-41-22-985
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Tel: 3-3495-8319
Fax: 3-3495-5397
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Tel: 2-569-9161
Fax: 2-569-9092
Poland – Warszawa
Tel/Fax: 22-835 83 83
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Tel: 095 787-76-14
Fax: 095 787-76-15
Singapore
Tel: (65) 389-6500
Fax: (65) 389-6501
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Tel: 91-657-9876
Fax: 91-657-9836
Sweden – Lund
Tel: +46 (0)46 158400
Fax: +46 (0)46 320781
Switzerland – Basel
Tel: 061-638 39 00
Fax: 061-638 39 40
Taiwan – Taipei
Tel: 2-2545-5991
Fax: 2-2545-5990
United Kingdom –
Portsmouth
Tel: 023 92 302600
Fax: 023 92 302601
Lab-UK@pall.com
Visit us on the Web at www.pall.com/lab
E-mail us at Lab@pall.com
© 2003, Pall Corporation. Pall,
registered in the USA.
8/03, 3k, GN03.0736
, and Nanosep are trademarks of Pall Corporation. ® indicates a trademark
is a service mark of Pall Corporation.
PN33124