USD 2610
PallSep™ Biotech Systems
A gentle, efficient separation technology for complex
biotech harvest and clarification applications
Vibrating Membrane Filtration
(VMF) technology prevents the
accumulation or build-up of
retained species at the
membrane surface and
subsequent membrane fouling.
Requires much lower cross flow
and transmembrane pressure
(TMP) than traditional TFF
technologies, resulting in lower
energy consumption and allowing
better protein transmission.
Offers gentle product treatment,
even with high suspended
solids levels.
Simplifies scale up. Filter modules
are available in 0.2, 1 and 5 m²
surface areas for more flexibility
and easy scale up or scale down.
Encapsulated module is easy
to use and replace.
Expands processing options.
Users may combine different
process steps within the same
system (for example, harvest
and clarification steps).
Compact design reduces the
system footprint.
Applications
Concentration, clarification and
purification of high particle load,
high viscosity or difficult-to-filter
solutions such as:
Fermentation broths
Lysates
These solutions are used in the
production of high value products,
for example, therapeutic vaccines
or antigen extracts.
Description
Technical Specifications
Tangential flow filtration (TFF) is well established in biotechnological
applications for the concentration and diafiltration of proteins
and cell harvest. However, the energy input to achieve the
required cross flow rates is high and the generated shear effect
can damage sensitive target molecules. Innovative dynamic
filtration systems using microporous membranes may be
used as an alternative.
Materials of Construction
The new PallSep Biotech System is suitable for the gentle
separation of target molecules from complex process fluids
containing high levels of biomass/particulates and high viscosity.
The shear forces generated by Vibrating Membrane Filtration
(VMF) are limited to a narrow layer on the membrane surface,
thus the bulk of the process fluid is not exposed to the shear.
In addition, the required cross flow is much lower when
compared to other TFF technologies. The system allows for a
dynamic membrane filtration with lower energy input and gentle
processing of the target molecule with a low pressure drop.
The PallSep Biotech System consists of encapsulated 0.2 µm
polyethersulfone (PES) filter modules with filter areas of 0.2 m²,
1 m² and 5 m², which allow for process development and
full-scale production. All critical GMP requirements for
biopharmaceutical processes are met without compromising
the ease of use, flexibility, cleanability, safety or operating costs.
The PallSep Biotech System uses an offset motor that induces
oscillation of two masses (the membrane module and a seismic
plate) connected by a torsion rod. Operation close to the resonance
frequency of ~50 Hz leads to a very low energy input to maintain
the oscillation of the two masses. The oscillation prevents
particulates from accumulating at the membrane surface and thus
fouling the membrane surface. A stable permeate flow is maintained
while achieving high levels of product transmission, even with high
particulate density and viscosity.
Designed to meet customer-specific requirements, the PallSep
Biotech System includes all the valves, pumps, sensors, vessel
and piping elements mounted on a skid assembly to operate
the unit, either in manual or automated conditions.
Quality Standards
Modules are manufactured under a system of Quality
Management certified to ISO 9001:2000 Quality System
Standard and 100% integrity tested during manufacture.
PallSep Biotech Module
Membrane
Membrane Support Plate
Membrane Drainage Layer
Spacers
Encapsulant
O-rings/Gaskets
Housing
Core
0.2 µm polyethersulfone
Polyolefin copolymer
Polyolefin
Polyester
Epoxy resin
EPDM
Fiber reinforced plastic
Polysulfone
PallSep Biotech PS50 Base Unit
Tubings and Fittings
Surface Finish
316 L stainless steel
≤ 0.5 µm internal
≤ 0.8 µm external
Platinum cured silicone
EPDM
Hoses with Integral Fittings
Gaskets
Operating Parameters
Maximum Operating
Pressure at Ambient Temperature
Maximum Operating Pressure
Maximum Transmembrane
Pressure (TMP)
Maximum Operating Temperature
Minimum Operating Temperature
4.2 barg (60 psig)
4.2 barg (60 psig)
3.5 barg (50 psig)
50 ºC
4 ºC
Ordering Information
Part Number
Module Description
Pkg
VMFPSM20B02
VMFPSM20B10
VMFPSM20B50
0.2 m² PallSep Biotech module
1 m² PallSep Biotech module
5 m² PallSep Biotech module
1/pkg
1/pkg
1/pkg
Note: Each module is delivered with one set of the gaskets required for the
installation of the module into the PallSep Biotech PS50 base unit.
PallSep Biotech System
Please contact your Pall representative for more information
Each module is delivered with a certificate of quality.
Visit us on the Web at www.pall.com/biopharm
e-mail us at biotech@pall.com
United States
800.717.7255 toll free (USA)
516.484.5400 phone
516.801.9548 fax
biotech@pall.com e-mail
Europe
+44 (0)23 9230 3303 phone
+44 (0)23 9230 2506 fax
BioPharmUK@europe.pall.com e-mail
International Offices
Pall Corporation has offices and plants throughout the world in locations such as: Argentina, Australia, Austria,
Belgium, Brazil, Canada, China, France, Germany, India, Indonesia, Ireland, Italy, Japan, Korea, Malaysia, Mexico,
the Netherlands, New Zealand, Norway, Poland, Puerto Rico, Russia, Singapore, South Africa, Spain, Sweden,
Switzerland, Taiwan, Thailand, the United Kingdom, the United States, and Venezuela. Distributors in all major
industrial areas of the world.
The information provided in this literature was reviewed for accuracy at the time of publication. Product data may be
subject to change without notice. For current information consult your local Pall distributor or contact Pall directly.
© 2009, Pall Corporation. Pall,
, and PallSep are trademarks of Pall Corporation. ® indicates a trademark
registered in the USA. Filtration. Separation. Solution. is a service mark of Pall Corporation.
SM
10/09, PDF, UK GN09.2894
USD 2610