Needle-and-thread Page 1. Introduction: discussion of classification, seral assignment and monitoring. Page 2. Graph. Page 3. Instructions: plot setup and data collection using the Excel spreadsheet. Page 4. References. Caution Needle-and-thread.xls is an Excel workbook containing the datasheet for field data collection. Copy the Excel file to a PDA. The .xls file will be converted to a Pocket Excel file with a .pxl extension. The file contains a spreadsheet with formulas that provide calculations necessary for classification of a site, using the variables defined for this ecological type. Be careful with the spreadsheet when loaded on a PDA. Although the formulas and cells are protected on the PC version of Excel, protection of spreadsheets/worksheets is not an option on the Pocket version of Excel. It is possible to erase formulas and stop the classification system. Be sure to make changes only within the empty cells, and not in any of the calculation or formula cells such as cov%, probabilities, etc. In addition, always: • Keep a backup copy of the original ‘Needle-and-thread.pxl’ file on your PDA and, • Save the file under a new name after finishing data collection on each site, e.g. ‘Needle-and-thread site1.pxl’, ‘Needle-and-thread site2.pxl’… to avoid overwriting previous files with data. Monitoring Seral Stages in Needle-and-thread-Western wheatgrass-Blue grama Ecological Type Classification, seral assignment, and monitoring of needle-and-thread-western wheatgrass-blue grama ecological type seral stages is according to the method of Uresk (1990). Statistical analysis of field-collected data indicates the presence of four seral stages: early, early intermediate (EINT), late intermediate (LINT) and late. A site is classified, and assigned to a seral stage by entering a set of key plant indices into the set of canonical discriminant functions developed for this ecological type and estimating posterior probability for each seral stage (SPSS, 1995). The site monitored is assigned to the stage with the highest posterior probability. For the needle-and-thread-western wheatgrass-blue grama ecological type, the key species are Stipa comata (STCO), Agropyron smithii (AGSM), and Bouteloua gracilis (BOGR). Indices for these species are developed from data collected using the Daubenmire (1959) canopy cover method. This method requires field collection of canopy cover and frequency of occurrence for each of the three plant species. The index is produced by multiplying total cover by frequency of occurrence (as a percent) for each species for the overall site. The seral stage assigned to a set of plant indices is always associated with the greatest probability value. If that value is 1, then succession is at the middle of the assigned seral stage. If the greatest probability value is less than 1, then the second greatest probability value is used to determine the successional status relative to middle of the assigned stage. For instance, if the second greatest probability is associated with an earlier seral stage, succession is at the lower end of the assigned seral stage. On the other hand, if the second greatest probability is associated with a later seral stage, succession is at the upper end of the assigned stage (see example below). When permanent macroplots are established to assess the vegetational trend of a site, re-sampling and comparing the previously assigned seral stage to the current stage provides information about succession or retrogression. On a finer scale, within-stage changes in vegetation successional status can be quantified by comparing the probability values. For example, first-year field measurements collected for canopy cover and frequency of occurrence on a site resulted in the following index values: STCO=2500, AGSM=300, and BOGR=2400. Four years later the index values obtained were: STCO=2500, AGSM=400, and BOGR=2700. Calculating the probabilities, we find: STCO AGSM BOGR LATE LINT EINT EARLY Stage Year1 2500 300 2400 .058 .000 .593 .402 EINT Year5 2500 400 2700 .028 .000 .858 .114 EINT Difference In both years, the site was classified and assigned to the early intermediate seral stage; the greatest probability values (.593 in Year1 and .858 in Year5) are associated with EINT. Placement within the early intermediate seral stage is at the lower end of the stage, as indicated by the second greatest probability values (.402 for Year1 and .114 for Year5) which are associated with an earlier seral stage (EARLY). The site shows an upward successional change of about .27, which is the difference between the probabilities in Year1 and Year5. The figure to the right illustrates this example. These quantitative changes in the probabilities can be used to evaluate and monitor the effects of management alternatives. .265 Late Late Intermediate Early Intermediate .59 Early Year 1 1.0 .5 Late 1.0 .5 Late Intermediate 1.0 1.0 .5 Early Intermediate .86 Early Year 5 1.0 .5 Late 1.0 .5 1.0 Early Intermediate .27 Late Intermediate 1.0 .5 Early Change 1.0 .5 1.0 .5 1.0 .5 1.0 Page 1 of 4 Monitoring Seral Stages in Needle-and-thread-Western wheatgrass-Blue grama Ecological Type Needle-and thread-Western wheatgrass-Blue grama Ecological Type by Seral Stages Mean Index Values of Key Plants 6000 Needle-and-thread Western wheatgrass Blue grama 5000 Index Values 4000 3000 2000 1000 0 LATE L. INTERMEDIATE E. INTERMEDIATE EARLY Mean Index Needle-and-thread Western wheatgrass Blue grama 5,271 626 577 3,226 3,538 1,249 2,413 347 3,730 1,319 225 1200 Mean indices of plant species through four seral stages of succession in a needle-and-thread-western wheatgrass- blue grama ecological type. Smoothed lines connect the means for each key plant species at each stage. Graph provides a guide for an approximate mixture of species at each seral stage. Page 2 of 4 Monitoring Seral Stages in Needle-and-thread-Western wheatgrass-Blue grama Ecological Type 1. Establish two parallel 30 m permanent transects, 20 m apart, as a plot within the needle-and-thread-western wheatgrass-blue grama ecological type. Recommendations are to establish two plots per section within the ecological type. 2. Using the ‘Needle-and-thread.xls’ spreadsheet (see figure at left) installed on your PDA, record canopy cover within a 20cm x 50cm quadrat for Stipa comata (STCO), Agropyron smithii (AGSM) and Bouteloua gracilis (BOGR) at one-meter intervals along each transect (30 readings/transect) using Daubenmire (1959) classes 1 through 6. Class Code 1 2 3 4 5 6 Cover Class >0 - 5% 5 - 25% 25 - 50% 50 - 75% 75 - 95% 95 – 100% Mid Point 2.5% 15% 37.5% 62.5% 85% 97.5% The spreadsheet calculates percent canopy cover, frequency-of-occurrence, and index values for each plant species for each transect and for the site. It also provides the classification probabilities by seral stage and the seral stage assignment. Needle-and-thread.xls Calculations Cover (%) = Sum Daubenmire midpoint values for each species per transect, divide by 30. Site cover = mean cover of the 2 transects. Frequency (%) = Divide the number of plot frames in which the species is present by the total number of plot frames on the transect. Convert that value to a percent by multiplying by 100. Site frequency = mean of the 2 transects. Index = the Site Cover mean times the Site Frequency mean. ((Xsect 1 cover + Xsect 2 cover) / 2) x ((Xsect 1 freq + Xsect 2 freq) / 2) Page 3 of 4 Monitoring Seral Stages in Needle-and-thread-Western wheatgrass-Blue grama Ecological Type REFERENCES Daubenmire, R. 1959. A canopy-coverage method of vegetational analysis. Northwest Science. 33(1):43-64. SPSS 12.0 for Windows. 1989-2003. SPSS Inc. 444 N Michigan, Ave, Chicago, IL 60611. Uresk, D. W. 1990. Using multivariate techniques to quantitatively estimate ecological stages in a mixed grass prairie. J. Range Mgt. 43(4):282-285. Page 4 of 4