Analytical Techniques (Chemistry 442G) Lecture and Laboratory Syllabus
Spring 2016
Lecture Instructor: Dr. E. McJimpsey, Department of Chemistry, Visiting Professor
Office: Currens Hall 519D, phone number: 309.298.3271, and e-mail: EL-Mcjimpsey@wiu.edu
Office Hours: T & R. 9:00-11:00am
Lecture: MWF 12:00-12:50pm, Currens Hall 202
Lab Instructors: Dr. E. McJimpsey (January 19-March 3, 2016)
Dr. L. Song, (March 8-April 26, 2016)
Currens Hall 324B, office hours: MWF 10-11am, e-mail: L-song@wiu.edu,
phone number: 309.298.656
Laboratory: TA: Sangeetha Mylapurapu (s-mylapurapu@wiu.edu)
Section 21- Tues. 11:00am-1:50pm, Currens Hall 419
Section 22- Thurs.11:00am-1:50pm, Currens Hall 419
Chem 442G Prerequisite: Completion of Chem 341.
Required Materials:
•
Textbook. “Undergraduate Instrumental Analysis”, 7th edition, by James Robinson, Eileen
Frame, and George Frame. CRC Press. ISBN: 978-1-4200-6135-2.
•
Course Syllabus: PDF file downloadable from Western Online
•
Official Laboratory Research Notebook-50 pages (Available at the WIU bookstore.)
•
Material Safety Data Sheets (MSDS) information about all chemicals utilized in the
laboratory can be found at the following web site.
http://avogadro.chem.iastate.edu/MSDS/. E-copies are free of charge.
•
Approved safety goggles are required for the Chem 442G laboratory.
•
Scientific calculator
•
Laboratory usage fee of $35
Testing and Grading:
Three 50 min exams and a comprehensive final will be given. The lowest score of the four exams
will be dropped. The final exam can be used as a make-up exam, with an excused absence, for one
and only one missed lecture exam. Several in-class assignments that are equally weighted will be
given throughout the semester. Quizzes will be given on Fridays at the beginning of each class
period. To accommodate absences, the lowest quiz score will be dropped. Make-up quizzes will
not be given. Ten laboratory experiments will be conducted; the highest nine scores will be used to
determine the final grade. Two laboratory exams will be given.
Emerging Techniques and Technologies in Analytical Chemistry Presentation:
In groups of two or three, students will give a 20 min oral presentation (5 min questions) on a
peer-reviewed research article that focuses on a new and emerging technique or technology in the
field of analytical chemistry. An article published within the last 6 months from an analytical
chemistry journal (http://www.scimagojr.com/journalrank.php?category=1602) should be selected.
A copy (pdf) of the article must be emailed to the instructor for approval a minimum of three
weeks prior to the scheduled presentation date. Failure to do so will result in a loss of points. For
the presentation, a minimum of 8 power point slides should be composed and include the
following: article title, author names, journal name, background on the need for the new
technique/technology, application of the technique/technology, description of the testing
procedure, and results/discussion for the new technique/technology, as well as, the conclusions
drawn. Particular emphasis should be placed on the benefit new technique/technology. Your
presentation must include some elements of digital multimedia (images, charts, graphs, diagrams,
video, audio) to illustrate your spoken text. Provide references for all multimedia. Each student in
the group is expected to present a portion of the presentation. The final grade for the presentation
will be a composite score calculated from evaluations performed by your peers (30%) and the
instructor (70%). There will be no make-up presentations. An electronic copy of each article will
be posted on Western Online. Make sure that any movie files or sound files in your presentation
are included in a folder with your presentation file or they will not work. On the day of your
presentation, bring the presentation to class on a USB drive. A paper copy of your presentation
slides should also be brought as a back-up. Presentations will begin on Friday, February 19, 2016.
Some quiz questions will be garnered from the presentations.
Best 9 of 10 Quizzes: 15%
Classroom Assignments: 5%
Best 3 of 4 Exams: 35 %
Best 9 Laboratory Reports of 10: 30%
2 Laboratory Exams: 5%
Emerging Techniques in Analytical Chemistry Presentation: 5%
Laboratory Experiment Development Exercise: 5%
Course Grading Scale:
Course
Grading Scale Grade
Percentage (%)
95-100
A
91-94
A88-90
B+
84-87
B
81-83
B78-80
C+
74-77
C
71-73
C68-70
D+
64-67
D
61-63
D0-60
F
Tentative Lecture Schedule
Chapter #
Chapter Title
1
Concepts of Instrumental Analytical Chemistry
2
Introduction to Spectroscopy
5
Visible and Ultraviolet Molecular Spectroscopy
9 and 10.3.3
Mass Spectrometry I: Principles and Instrumentation
Exam #1, Mid/Late February (Chapters 1, 2, 5, and 9/10)
4
Infrared, Near-Infrared, and Raman Spectroscopy
6
Atomic Absorption Spectrometry
11
Principles of Chromatography
Exam # 2, Mid/Late March (Chapters 4, 6, and 11)
12
Gas Chromatography
13
Chromatography with Liquid Mobile Phases
15
Electroanalytical Chemistry
Exam #3, Late April (Chapters 12, 13, and 15)
May. 9-13, 2016, Finals Week, No Class
Tentative Laboratory Schedule
Date
Laboratory Experiments
Tues. (Thurs.), Jan. 19 (21), 2016 Check-In & Safety
Exp. #1, Comparing Transmittance and Absorbance
Tues. (Thurs.), Jan. 26 (28), 2016 of KMnO4 in UV/VIS Spectroscopy (Exp. 2.1 and
2.2, p. 108)
Exp. #2, Determination of L-Phenylephrine
Tues. (Thurs.), Feb. 2 (4), 2016
Concentration in Dristan Nasal Spray Using UV/VIS
Spectroscopy (Exp. 5.10, p. 433)
Exp. #3, Proteomics Virtual Laboratory, Currens Hall
Tues. (Thurs.), Feb. 9 (11), 2016
529 (handout)
Exp. #4, FTIR Absorption Comparison of Hexane
Tues. (Thurs.), Feb. 16 (18), 2016
and Heptane (Exp. 4.1 and 4.2, p. 348)
Exp. #5, Determination of Sodium Concentration in
Tues. (Thurs.), Feb. 23 (25), 2016 Soda Using Atomic Absorption Spectrometry (Exp.
6.3, p. 490)
Tues. (Thurs.), March 1 (3), 2016 Laboratory Exam 1
Exp. #6, Quantitative Determination of Limonene
Tues. (Thurs.), March 8 (10), 2016
Content Using Gas Chromatography (handout)
Exp. #7, Determination of Trace Impurities in
Tues. (Thurs.), March 22 (24), 2016 Whiskey Using Gas Chromatography Mass
Spectrometry (handout)
Exp. #8, Determination of Caffeine Concentration in
Tues. (Thurs.), March 29 (31), 2016
Urine Using HPLC (handout)
Exp. #9, Determination of Peptide Concentration
Tues. (Thurs.), Apr. 5 (7), 2016
Using HPLC (handout)
Exp. #10, The Effect of Temperature on pH
Tues. (Thurs.), Apr. 12 (14), 2016
Measurements (Exp. 15.7, p. 1131)
Tues. (Thurs.), Apr. 19 (21), 2016 Laboratory Exam 2
Tues. (Thurs.), Apr. 26 (28), 2016 Check-out
Important Dates:
Date (Spring, 2015)
January 18, Mon.
January 19, Tues.
February 12, Fri.
February 15, Mon.
March 14-18, Mon.-Fri.
March 21, Mon.
May 9-13, Mon.-Fri.
Event
Martin Luther King Day - University is closed
Classes Begin
Lincoln's Birthday - University is closed
Classes Resume
Spring Break - No Classes
Classes Resume
Final Exam Week
Safety:
Safety is of primary concern in the chemistry laboratory. Prior to each laboratory experiment,
please review the Material Safety Data Sheets (MSDS) for the hazards of the chemicals being
used in each experiment at the website, http://hazard.com/msds/. Additionally, safety goggles
must be worn at all times while in the laboratory, as well, as closed-toed shoes and pants
covering the entire shin. Shoulders must also be covered. Failure to adhere to these policies will
result in a student being asked to leave the lab. The lab will be considered as a missed session.
Laboratory Reports:
With the exception of one excused absence, each laboratory experiment and report is expected to
be completed. Laboratory reports are due one week after the scheduled completion of the
experiment. There will be a 12pt loss for each day (including weekends) it is late. The laboratory
reports should be submitted to the dropbox on Western Online on or before the scheduled lab
date; a paper copy should be submitted to the instructor on your lab day. Laboratory (pre- and
post-) reports will be analyzed by the software, TurnitIn, for plagiarism; post-laboratory TurnitIn
scores above 35% will result in an 80 % deduction in overall report points. Additionally, please
be aware that school policy requires notification of all cheating to the departmental chairperson.
If the report is late, place it in your instructor’s mailbox in the chemistry department office. No
late materials will be accepted beyond one week after the due date. Missing any scheduled
laboratory activities will result in a loss of points.
Report Format:
Pre- and post-laboratory reports are to be typewritten (Font: 12, Times New Roman and 1 inch
margins). Pre-laboratory reports should be done prior to the laboratory and must include a cover
page and the following 5 sections: objective of the experiment, experimental theory/background,
materials, procedures (in your own words), and reference. The final laboratory report should
include the revised pre-laboratory report sections, as well as, the: experimental results (including
calculations), discussion and conclusion, and answers to end of lab questions. A copy of the raw
data should be included at the end of the final report. Lab reports should represent each student’s
individual work. Participation in the laboratory requires completion of pre-laboratory report,
including all calculations needed for experimental sample preparation prior to the start of class.
Laboratory Report Section Formatting
A stand-alone cover page (2 pts) should be the first page of the laboratory report. It must
include the title of the experiment, course number, your name (bold type) and the name of your
lab partner, the names of your lab instructor and TA (for that experiment), and the date that the
lab was performed; the text should be centered in the middle of the page. Two to three sentences
about the objective (3 pts) of the experiment should be given. The theory/background section
(5 pts) should be no less than ½ of a page and summarize how the objectives will be realized. For
example, important reactions, techniques, or instrumental methods can be discussed; all sources
utilized used should be cited. The theory should not include a description of the procedure. All
chemicals, reagents (including concentrations), instruments, devices, and glassware should be
recorded in the materials section (5 pts). The procedure (methods) section (8 pts) should give a
description of the laboratory experiment that was performed and should be written in your own
words. Arabic numerals should not be used at the beginning of a sentence and the procedure
must be written in past tense and a passive voice. The results section (12 pts) should report
findings and include all tables, graphs, and calculations. A representative example for each
calculation should be labeled with a title, typewritten, and included in the in this section (i.e.
mean, standard deviation, etc). All data should be typed and organized in tables. Tables and
graphs should have titles and the x/y axis should be labeled. All calculations should include the
appropriate units. The discussion and conclusion section (12 pts) should be used to interpret the
findings, draw conclusions, and explain erroneous findings from the data that was collected and
analyzed. The reference section (3 pts) should be last and the citations should be formatted using
the American Chemical Society style.
Laboratory Experiment Development Exercise:
This assignment is only for students that are registered for the 442G course (graduate and bridge
students only). In this exercise, you will be required to develop two analytical chemistry
laboratories that can each be conducted during one 3 hour time period for 20 undergraduate
students. The experiments should include an objective and individual sections describing the
experimental background/theory, materials needed, procedure, calculations and questions that
should be completed by the student after completion of the experiment, expected outcomes from
the experiment (i.e. results), as well as, a bibliography. The experimental topics that you can
select from include size exclusion chromatography, time-of-flight mass spectrometry, raman
spectroscopy, and capillary electrophoresis. Each experiment will be worth 25 pts. These
experiments should be individually uploaded to the dropbox and submitted to TurnitIn on
Western Online. Plagiarism will result in a score of zero (score above 35 %). The first
experiment is due on March 11, 2016. The second experiment is due on April 29, 2016. On these
dates, a paper copy of the exercise should also be given to the instructor at the beginning of class.
Without exception, 10 points (weekends included) will be deducted for each day the assignments
are late.
Attendance Policy:
Students are expected to punctually attend all lecture and laboratory classes. A total of 3
excused and 1 unexcused absences are allowed. The WIU policy on class attendance can be
found at: http://www.wiu.edu/student_services/student_developme nt_office/ current/a bs ence
pol icy.php. An OARS report must be filed with each absence. Failure to report the absence will
result in a loss of exam points. Any student arriving more than ten minutes late for a quiz, exam,
or laboratory exercise will be allowed to take or complete the assigned work at the instructor’s
discretion. Any student who receives six or more absences may be dropped from this course
without notice.
Outside work requirements: Students are expected to read ahead and be prepared for class.
Reading the designated chapter before the class and going over the notes after each class is
recommended. A daily minimum of two hours of out of class work is needed to do well in this
class.
Classroom and Course Policies: Any student convicted of academic dishonesty will receive a
failing grade and may be subjected to further academic penalty, including expulsion. See the
WIU academic dishonesty policy 4 (http://www.wiu.edu/policies/acintegrity.shtml).
Emergency Preparedness: The WIU Office of Risk Management and Emergency Preparedness
provides resources on how to respond to emergency situations. Please view the video resources
at http://www.wiu.edu/rmep/.
Students with Disabilities:
In accordance with University values and disability law, students with disabilities may request
academic accommodations where there are aspects of a course that result in barriers to inclusion
or accurate assessment of achievement. To file an official request for disability-related
accommodations,
please
contact
the
Disability
Resource
Center
at
309-298-2512,
disability@wiu.edu or in 143 Memorial Hall. Please notify the instructor as soon as possible to
ensure that this course is accessible to you in a timely manner.
Students Rights and Responsibilities:
The WIU policy on the rights and responsibilities of students can be found at
http://www.wiu.edu/provost/students.php.