The eukaryotic cell cycle
Mitosis and cytokinesis
Blue-DNA
Green-microtubule
Mitotic apparatus
of mammalian cells
Fig. 19-36
Centrosome duplication and cell cycle
Microtubule motors and centrosome
movement at prophase
Fig. 19-43
Capture chromosomes
and stabilizing
chromosomes at the
equatorial plate in metaphase
Shortening at the (+) end
of kinetochore microtubules
moves chromosomes
poleward in anaphase A
Spindle elongation and movement of poles during anaphase B
Whether does the spindle or the asters
control location of the cleavage plane during cytokinesis?
Regulation of myosin during cytokinesis
MPF-mitosis promoting factor
Mitosis in a higher plant cell
Regulation of cell cycle
Protein factors
Mechanisms
Cyclin
Phosphorylation and
dephosphorylation
CDK-cyclin-dependent kinase
Cyclin complex
G1 cyclin-CDK complexes
S phase cyclin-CDK complex
Mitotic cyclin-CDK complex
Cell cycle inhibitors
Cell cycle promoting factor ubiquitin ligase lead to degradation
of cell cycle inhibitors
SCF and APC
Activation of gene
transcription and translation
Proteasome-mediated
degradation
Current model for
regulation of the
eukaryotic cell cycle
CDK-cyclin-dependent kinase
SCF-ubiquitin ligase
APC-anaphase promoting complex
A diffusible factor in arrested Xenopus eggs promotes meiotic maturation
Biochemical detection of cyclical synthesis and destruction of mitotic cyclin
in sea urchin embryos
Isolation of cell-division cycle (CDC) genes from a S. cerevisiae
genomic library by functional complementation of cdc mutants
Using S. pombe cdc mutants to study the cell cycle regulation
Regulation of the kinase
activity of S. pombe mitosispromoting factor by
phosphorylation and
dephosphorylation
Depolymerization of the nuclear lamina
The phosphorylation of human lamin a is required for lamin
depolymerization
Control of entry into anaphase by APC-regulated degradation of
the cohesin link between sister chromatides
The reassembly of nuclear
envelop during telophase
Mammalian cyclin-CDK complexes
Using bromodeoxyuridin (BrdU) to test the
function of cyclin D in mammalian cells
Checkpoint controls in cell cycle
Role of p53 in cell cycle check points