2/7/2008 Seed Structure Grass Seed • Collection of cleaned, mature florets Matured Florets Flowering Floret • Grass Flower – Three stamens • Each with one anther and one stigma – One ovary – Two feathery stigmas – Two lodicules at the base Bluegrass Fescue Ryegrass Bentgrass • Two bracts – Palea and lemma 1 2/7/2008 Matured Floret • Two bracts enclose the fruit – Caryopsis developed from the ovary Caryopsis • Dry fruit • Ovary wall (pericarp) fused to seedcoat Caryposes Spikelet • Florets attached by its base (callus) to the rachilla • Two bracts (glumes) at lower end of the spikelet Fescue Bluegrass Bentgrass Spikelet • Bentgrass: one floret and two glumes • Ryegrass: up to 20 florets and one glume Inflorescence • Spikelet borne in inflorescence Open Compressed Spike Panicle Panicle 2 2/7/2008 Inflorescence “Floret” Identification • Transition from vegetative to floral growth and development – Stem elongation I fl Inflorescence Floret Directions • Front view – Palea is facing toward you. – Rachilla is seen arising from the callus base. • Side view Rachillae • Stem-like segment – Protrudes up from the callus on the palea side – Missing in bentgrass floret – Side of the lemma is seen as it curves about the palea. • Back view – Lemma faces toward you Bluegrass Fescue Ryegrass Bentgrass Nerves on Lemma • Lateral nerves along the edges of the lemma • Mid-nerve running down the middle of the lemma from apex to base base. Awn • Arise from the mid-nerve halfway from apex to base. – Absent in the bluegrass. Fescue Ryegrass Bluegrass Bentgrass 3 2/7/2008 Pubescence (hairs) on the floret • Types – Wispy masses to individually spaced stiff hairs. Seed Identification • Location L i – At the base, on the lemma, or along any of the nerves. • Maybe lost during cleaning Ryegrasses and Fescues • Largest florets – 6mm average length Ryegrasses and Fescues • Sample Size: 10 florets • Magnification: Hand lens Ryegrasses and Fescues • Ryegrass rachilla – Wide and flattened against the palea. – Oblong g top p • Fescue rachilla – Narrow cylindrical – Circular upper end and somewhat flared 4 2/7/2008 Ryegrasses and Fescues • Floret Shape – Ryegrass rectangular appearance – Fescue somewhat elliptical Ryegrasses and Fescues • Inflorescence – Ryegrass spikes – Fescue panicels • Spikelets p – Ryegrass 6-20 florets – Tall fescues 6-8 florets – Fine fesuce 4-7 florets Ryegrasses • Long, slender awn on annual ryegrass Bluegrasses • Medium size floret – 2.5 mm average length • Fine Fescue Fescues • Tall fescue – Wide and flattened front to back – Awn may be present – Narrow and cylindrical – Floret arches away from the palea side – awn appears as a narrowed extension of the whole lemma Bluegrasses • Sample size: 25 florets • Magnification: hand lens or dissecting scope 5 2/7/2008 Bluegrasses Bluegrasses • Inflorescence – Open to compressed panicles – 2 to 3 florets per spikelet in rough bluegrass – 3 to 6 florets in other species Poa annua Bluegrasses • Canada bluegrass – Smooth lemma • No visible intermediate nerves – Widening of the lemma above the middle Bluegrasses • Kentucky bluegrass – Pubescence completely removed – Lemma edges tend to curve – Lemma edges wrapped more weakly about the palea Poa compressa Poa pratensis Poa trivialis Bluegrasses • Rough bluegrass – Remnant of basal pubescence – Prominent intermediate nerves – Lemma straight along its edges – Edges of the lemma folded over the palea. Bluegrasses • Annual bluegrass – Very prominent intermediate,lateral and midnerves – Pubescence may be seen along any or all of these nerves in abundance – Palea and lemma appear to be sucked in tightly 6 2/7/2008 Bentgrasses • Very small florets – 1.5mm average in length Bentgrasses • Can be difficult to identify • Sample Size: 50 florets Bentgrasses • Inflorescence open or compressed Agrostis alba Agrostis Agrostis Agrostis Cania palustris tenuis Bentgrasses • Spikelet – One floret and two glumes • Typical bentgrass sample – May contain several spiklelets • Size, color, retained pedicels 7 2/7/2008 Bentgrasses • Velvet bentgrass – Most awns entact • Bent awn, arsing from mid-rib about one half of the wayy from base to apex p • Five nerves below awn’s point of attachment, four above. Bentgrasses • Colonial bentgrass – Shiny paleas and lemmas – 3 or 5 nerves visible – Variable length g of p palea Stiff hairs arising g from the lemma surface – Awn originating from near the callu – Palea is rudimentary – Lemma grainy Bentgrasses • Creeping bentgrass – Awn rarely present • Remaining stub on the mid-nerve toward the apex. – – – – 5-nerved lemma is more often Palea longer than the caryopsis Stubby basal hairs Shinny bract Bentgrasses • Redtop, – – – – Difficult to distinguish from creeping BG More often 3-nerved Usuallyy longer g basal hair Palea as long as the caryopsis Seed Identification • Many varieties deviate in floret phenotype (outward physical characteristics) from the norm for that species Seed Quality • Purity – Percentage of pure seed of an identified species or cultivar – Remaining percentage • inert matter, weed seeds, and other crop seeds • Viability – Percentage of seed that is alive and will germinate under standard conditions 8 2/7/2008 Seed Quality Seed Quality • Pure Live seed (PLS) • Product of purity and viability – 92% Purity x 80 %Viability = 73.6 % PLS. • Compare seed prices Actual cost of pure live seed = Cost per pound of seed x 100 PLS % 9