LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
FIRST SEMESTER – NOVEMBER 2012
BT 1825 - MOLECULAR BIOLOGY & MOL. GENETICS
Date : 01/11/2012
Time : 1:00 - 4:00
Dept. No.
Max. : 100 Marks
PART – A (20 marks)
ANSWER ALL THE QUESTIONS
1. Choose the correct answer:
(5 x 1 = 5 marks)
1) Which is the correct genome size of E.coli?
a) 4.639 Mb
b) 7 Mb
c) 10 Mb
d) 2 Mb
2) Where does translation occur in a eukaryotic cell ?
a) Cytoplasm
b) Nucleus c) Endoplasmic reticulum d) Mitochondria
3) Which of the following is not a stop codon?
a) AUG
b) UAA
c) UGA
d) UAG
4) Which of the following is a receptor ?
a) protein
b) cAMP c) DAG d) fatty acid
5) How many linkage groups are present in human female?
a) 23
b) 24
c) 25
d) 26
II. State whether the following are true or false; if false give reason:
(5 x 1 = 5 marks)
6) Alphoid satellite DNA is present in human chromosomes.
7) The size of Eukaryotic ribosome is 80S.
8) Recombination model was first proposed by Francis Crick.
9) cAMP is a second messenger.
10) Four strand double cross over gives 100% recombinants.
III. Complete the following:
(5 x 1 = 5 marks)
11) SAR stands for _________________.
12) CAAT box is present in ____________.
13) Euchromatin has _____________ chromatin.
14) Phagocytosis is the internalization of _______________ particles.
15) The binomial of the plant used by Mendel for his experiments is_________ _________.
IV. Answer following, each within 50 words only
(5 x 1 = 5 marks)
16) What are repetitive sequences?
17) Define Hogness box.
18) Define genetic code.
19) What are carrier proteins?
20) What is a test cross?
PART – B
Answer the following each within 500 words only; Draw diagrams wherever necessary:
(5 x 8 = 40 marks)
21 a) What is satellite DNA and how do you isolate it?
OR
b) Ilustrate the lac operon organization in E.coli.
22 a) Explain the galactose metabolism in yeast.
OR
b) Write a note on the various types of RNA polymerases in eukaryotes.
23 a) Distinguish between hetero and euchromatin.
OR
b) Give an account on site specific recombination.
24 a) Write a note on membrane proteins.
OR
b) Discuss enzyme linked receptor.
25 a) Cite the law of independent assortment with an example.
OR
b) Enumerate the characteristics of multiple genes.
PART – C
Answer any TWO of the following, each within 1500 words only; Draw diagrams wherever
necessary:
(2x 20 = 40 marks)
26) Describe the prokaryotic genome organization.
27 Explain G- protein linked signal transduction.
28 Illustrate semiconservative mode of DNA replication.
29 Explain the cytoplasmic inheritance seen in Paramecium.
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