Shark Dissection Lab Name: ____________________________________ Date: ______________________ Overview In this lab you will dissect a shark named the Dogfish Shark. You will perform this dissection so that you can gain a better understanding of the internal structures and processes of the shark in order to compare it to what you have learned about the human body systems. Materials a large dissection tray surgical scissors scalpel probe forceps gloves goggles Pre-Lab Questions 1) How will you make sure you are handling your dissection tools safely? ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 2) What should you do if you feel uncomfortable at any point during the lab? ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 3) Why is it important that we keep our voice level down as we work on the dissection? ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 4) What is the purpose of this lab today? ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ Shark Dissection Lab Procedure/ Observations/ Analysis STEP 1 Getting to know your shark □ Touch the shark! Pick it up, squeeze it, feel it! Slowly and carefully run your hand along the shark's body, from head to tail. Notice the difference in texture. The roughness that you feel is the shark’s scales. Part of shark Description Shark’s skin 1) Does it feel like the shark has hard bones similar to the bones that humans have? Explain. _______________________________________________________________ _________________________________________________________ _________________________________________________________ STEP 2 External Anatomy Use the diagram below to help you locate the following structures. Once you have located it put a check next in the box. □ External Nares – These are a pair of openings (nostrils) on each side of the head, in front of the eyes. Water is taken into the smaller of the two openings and expelled through the larger opening. The water passes by a sensory membrane allowing the shark to detect chemicals in the water. □ Spiracles – These are small openings behind the eyes. These openings allow water to pass through the gills even when the shark’s mouth is closed. □ Mouth – Although the eating function is evident, the mouth is also used for the intake of water that passes through the gills. □ Gill Slits – Five vertical slits which allow water to exit after passing over the gills. They are located behind; the mouth. □ Lateral Line – A pale line that extends noticeably from the side fin (pectoral fin) fin past the stomach fin (pelvic fin). This line is actually a group of small pores which open into the underlying lateral line canal, a sensory organ that detects water movements. Shark Dissection Lab □ Cloaca – This is the exit from the digestive tract. The cloaca lies between the stomach (pelvic) fins. □ Fins – Refer to Figure 1 and familiarize yourself with each fin and its name. □ Rostrum – This is the pointed snout at the front end of the head. □ Dorsal Spines – Just in front of each top fin (dorsal fin)is a spine that is used defensively by the shark. Each spine has a poison gland associated with it. Write down one thing you noticed about the Shark’s skin or external features. _______________________________________________________________ _______________________________________________________________ _______________________________________________________________ STEP 3: Skeletal system. Unlike the other ‘higher vertebrates’ (fish, reptiles, birds, etc.) the shark does not have a bony skeleton but instead has a skeleton composed of cartilage. □ Look at figure 2 which shows a side view of the entire shark skeleton and familiarize yourself with the parts outlined within this figure. Shark Dissection Lab How is the shark’s skeletal system different than a human’s skeletal system? ________________________________________________________________________ ________________________________________________________________________ STEP 4: Muscular System □ Place your shark stomach down to begin. You will need to flip the shark over after step one to complete this section. □ Remove each of the dorsal spines by cutting where it meets the body. (This will prevent you from stabbing yourself unintentionally). □ Flip your shark over onto its back. Be sure to refer to the diagram as you begin cutting into the skin. □ Make an incision on the shark’s stomach from the cloaca to just below the jaw/mouth. Make your incision shallow (not deep) □ Cut around the head, around each fin, around the spircles, and around the cloaca. □ From the cloaca cut a circle around the back of the shark – this will make a circle around the tail. Remember you are cutting through the skin only. □ Using the handles of your scissors or your gloved fingers carefully peel off the skin to expose the muscles. □ Compare your specimen with Figure 3 and Figure 4 on the next page. □ Try to identify as many of the structures listed as possible. Shark Dissection Lab Shark Dissection Lab What is one thing that surprised you when you looked at the different muscles in the shark? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ Compare and Contrast the muscles of the shark to the muscle of the chicken wing we dissected earlier in the year. STEP 5: Digestive System □ Place your shark stomach side up on the dissection tray. □ Using scissors – blunt tip inside the shark – cut through the muscle tissue. Some cuts may be difficult. Ask if you need help. Use the video to guide you. □ You may pin the flaps of muscle tissue if you would like. Shark Dissection Lab Use the diagram on the next page to help you identify the different digestive organs you see. Record your observations in the chart below. Digestive Organ Function Esophagus Connects the mouth to the stomach Stomach Duodenum (part of small intestine) Begins to break down food Continues to break down the food and absorb the nutrients Description Drawing Shark Dissection Lab Liver Secretes bile to help break down food. Gall Bladder Stores the bile Pancreas Creates enzymes used for digestion. Spiral Intestine (small intestine) Rectum Absorbs the nutrients from the food Stores solid waste before it is eliminated from the shark STEP 6: Circulatory System □ Lift the flaps over the area of the heart and pin them where they stay out of the way. It may be necessary to cut some tissue that may be attached to the heart. Draw a picture of the outside of the shark’s heart below. Shark Dissection Lab □ Cut open the heart to get a look at the different chambers. Draw a picture of the inside of the shark’s heart below. How is the shark’s heart similar and how is it different than a human heart? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ STEP 6: BONUS: Nervous System AKA THE BRAIN □ Remove the skin from the back section of the head. □ With your scalpel, carefully shave the chondocranium (shark’s cranium) down to expose the brain, the olfactory lobes, and the major brain nerves. Shave off thin sections so that you don’t cut into the brain or nerves. □ Remove chips of cartilage with forceps. Remove the chondocranium from the tip of the rostrum back to the gill slits STEP 7: Clean-Up □ Place all parts of organism that may have been removed into your dissection tray. □ When the teacher comes around place all parts and the organism into the bin. □ Using a wet paper towel carefully wipe off your dissection tools and the tray. □ Place all clean materials into the dissection tray. Shark Dissection Lab Dissection Reflection Questions 1) What organ systems did you observe today? ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 2) What were some similarities between the shark’s organ systems and the human organ systems? ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ _______________________________________________________________________ 3) What were some differences that you noticed between the shark’s organ systems and a human’s organ systems? ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 4) What were some things that surprised you today when you dissected the shark? ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 5) Did you enjoy this lab? Why or why not? ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________