Practical Biochemistry1 Lab BIOCA2101 IUG, Spring 2015 Dr. Tarek M Zaida 1 • Instructor Info Dr. Tarek M. Zaida Lab. Building C300 Phone: Office:2604 Mobile: 059-7193442, WhatsApp, Viber Email: tzaida@iugaza.edu • Office hours: TBD 2 Course Description • Introduction of students majoring in Biochemistry to some of the most widely used experimental procedures in biochemistry. • During this course students will become familiar with the principles of basic biochemistry through the very carefully selected lab activities that will focus on the fundamental biomolecules of the living system such as: amino acids, proteins, carbohydrates, and lipids 3 • This lab will focus mainly on: Qualitative tests of Carbs., Amino acids, Proteins, and Lipids Using different widely applied methods in biochemistry labs. 4 • Prerequisite: • Practical Analytical Chemistry CHEM 2110 • Biochemistry I BIOCA2301 5 Course Objectives • By the end of this course students will be: • Ready to explain the different roles of the very important biomolecules in the living system, through understanding the biochemical properties of such compounds. • More self-confident in conducting experiments with their own hands. 6 • Able to handle the different lab equipments with confidence. • Familiar with the different methods applied in basic biochemistry labs. • Students will: • Improve their communication skills • Get used to a team-work. 7 Textbook • Laboratory Manual in Biochemistry1 (Dr. Ahmed Thabet, 2005) 8 Evaluation • There will be different types of evaluation that will include: • Reading the lab manual before the lab activity day • Answering the pre-lab questions during lab session, • Quizzes (4) • Writing Lab reports • Participation in the actual lab work. • Participation in the discussion with classmates through the electronic learning tools. 9 Course Policy • Attendance all lab sessions is a mandatory. • Zero tolerance for not following given instructions during lab sessions. • Wearing the lab coat as soon as you step in the lab. • Acting responsibly during the entire lab session. • Team work is a must, including communication with group partners. • Giving up reports from the previous lab session must be electronically and before the day of the next lab. • Quizzes will be given only electronically on a previously announced date and time. • No make ups are allowed. 10 Grading Activity Points Participation in electronic learning tools 6 Pre-lab quizzes (11) 11 Quizzes (4) 16 Lab Reports 30 Active Engagement during lab Session 20 Practical Exam (in-lab) 17 Total grades 100 11 • Following up with the Course Students may reach the course materials through moodle system anytime. In case of anticipating any type of problems, student may contact me at my mobile or via email. • How to use moodle system Students may go through the moodle system using guide found on the website of electronic learning http://elearning.iugaza.edu.ps , under educational resources for students. Students may also ask for assistance from the electronic learning center found in the IT building I400, phone 1573. 12 Course Timing Activity Lab Activity 1 Carbohydrates Lab Activity 2 Reactions of Reducing Sugars Lab Activity 3 Phenyl Hydrazine Reaction Lab Activity 4 (Quiz 1) Paper Chromatography of Sugars Lab Activity 5 Lipids Lab Activity 6 Saponification Lab Activity 7 (Quiz 2) Proteins I Lab Activity 8 Proteins II Lab Activity 9 Precipitation of Proteins Lab Activity 10 (Quiz 3) Lab Activity 11 Quiz 4 (lab 10 + 11) Enzymes Metabolism 13 • Let’s get started.. …have fun working biochemistry 14