in-lab

advertisement
Practical Biochemistry1 Lab
BIOCA2101
IUG, Spring 2015
Dr. Tarek M Zaida
1
• Instructor Info
Dr. Tarek M. Zaida
Lab. Building C300
Phone: Office:2604 Mobile: 059-7193442,
WhatsApp, Viber
Email: tzaida@iugaza.edu
• Office hours: TBD
2
Course Description
• Introduction of students majoring in Biochemistry
to some of the most widely used experimental
procedures in biochemistry.
• During this course students will become familiar
with the principles of basic biochemistry through
the very carefully selected lab activities that will
focus on the fundamental biomolecules of the
living system such as:

amino acids, proteins, carbohydrates, and
lipids
3
• This lab will focus mainly on:
Qualitative tests of Carbs., Amino acids,
Proteins, and Lipids
Using different widely applied methods in
biochemistry labs.
4
• Prerequisite:
• Practical Analytical Chemistry CHEM 2110
• Biochemistry I BIOCA2301
5
Course Objectives
• By the end of this course students will be:
• Ready to explain the different roles of the very
important biomolecules in the living system,
through understanding the biochemical
properties of such compounds.
• More self-confident in conducting
experiments with their own hands.
6
• Able to handle the different lab equipments
with confidence.
• Familiar with the different methods applied in
basic biochemistry labs.
• Students will:
• Improve their communication skills
• Get used to a team-work.
7
Textbook
• Laboratory Manual in Biochemistry1 (Dr.
Ahmed Thabet, 2005)
8
Evaluation
• There will be different types of evaluation that will
include:
• Reading the lab manual before the lab activity day
• Answering the pre-lab questions during lab session,
• Quizzes (4)
• Writing Lab reports
• Participation in the actual lab work.
• Participation in the discussion with classmates through
the electronic learning tools.
9
Course Policy
• Attendance all lab sessions is a mandatory.
• Zero tolerance for not following given instructions
during lab sessions.
• Wearing the lab coat as soon as you step in the lab.
• Acting responsibly during the entire lab session.
• Team work is a must, including communication with
group partners.
• Giving up reports from the previous lab session must
be electronically and before the day of the next lab.
• Quizzes will be given only electronically on a previously
announced date and time.
• No make ups are allowed.
10
Grading
Activity
Points
Participation in electronic learning tools
6
Pre-lab quizzes (11)
11
Quizzes (4)
16
Lab Reports
30
Active Engagement during lab Session
20
Practical Exam (in-lab)
17
Total grades
100
11
• Following up with the Course
Students may reach the course materials through
moodle system anytime.
In case of anticipating any type of problems, student
may contact me at my mobile or via email.
• How to use moodle system
Students may go through the moodle system using
guide found on the website of electronic learning
http://elearning.iugaza.edu.ps , under educational
resources for students. Students may also ask for
assistance from the electronic learning center found in
the IT building I400, phone 1573.
12
Course Timing
Activity
Lab Activity 1
Carbohydrates
Lab Activity 2
Reactions of Reducing Sugars
Lab Activity 3
Phenyl Hydrazine Reaction
Lab Activity 4 (Quiz 1)
Paper Chromatography of Sugars
Lab Activity 5
Lipids
Lab Activity 6
Saponification
Lab Activity 7 (Quiz 2)
Proteins I
Lab Activity 8
Proteins II
Lab Activity 9
Precipitation of Proteins
Lab Activity 10 (Quiz 3)
Lab Activity 11
Quiz 4 (lab 10 + 11)
Enzymes
Metabolism
13
• Let’s get started..
…have fun working biochemistry
14
Download