Register Number
SATHYABAMA UNIVERSITY
(Established under section 3 of UGC Act,1956)
Course & Branch : B.Tech - BTE
Title of the Paper : Biology of Cloning Vectors
Sub. Code : 623701 (2007-2008)
Date :06/03/2012
Max. Marks:80
Time : 3 Hours
Session :AN
______________________________________________________________________________________________________________________
1.
PART - A
(10 x 2 = 20)
Answer ALL the Questions
What are the characteristics that a vector must possess?
2.
Define- Cosmids.
3.
Draw the genomic map of cauliflower mosaic virus?
4.
List out any four properties of a shuttle vector?
5.
What are the marker genes associated with plasmids?
6.
What is plasmid rescue technique?
7.
Differentiate the stringent and relaxed mode of plasmid
replication?
8.
How are cosmids advantageous than plasmids?
9.
List out the applications of Vaccinia viral vectors?
10. What are the possible advantages of using Ri plasmid in plant
systems?
PART – B
(5 x 12 = 60)
Answer All the Questions
11. Describe the types and mode of action of restriction
endonucleases?
(or)
12. Discus about various bacterial plasmids that are used in cloning
of foreign DNA?
13. Explain the mechanisms of following specialized vectors
involved in gene transfer?
(a) expression vectors
(b) off vectors
(or)
14. Discus in detail about the yeast plasmid vectors?
15. Describe the functional organization and replication of plasmids.
(or)
16. How is the plasmid pBR322 constructed? Add a note on its
derivatives.
17. Explain how are lambda phages applied as insertion and
replacement vector?
(or)
18. Write a note on biology of M13 vectors? Explain how are M13
vectors used in DNA sequencing studies?
19. Describe in detail about gene cloning in animal cells using SV40
vector?
(or)
20. Explain the mechanisms involved in the Ti plasmid based gene
transfer in plants?