PROJECT LIFE12 ENV/IT/00036
“Anti-infective environmental friendly
molecules against plant pathogenic bacteria
for reducing Cu”
“AFTER Cu”
07/07/2014
6 Month meeting
MURCIA - SPAIN
CEBASCSIC premises
Start: 01/01/2014
End: 31/12/2015
PROJECT LIFE12 ENV/IT/00036
AGENDA
6 MONTH MEETING
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09:30 [CEBASCSIC] Welcome and opening of the
meeting
09:35 [All] Actions carried out by each partner
(please prepare a ppt presentation and include
After-Cu logo and LIFE logo in each slide) and
scheduled activities till the end of June 2014.
10:30 [All] Open discussion on actions carried out
PROJECT LIFE12 ENV/IT/00036
AGENDA
6 MONTH MEETING
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•
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10:45 [All] After-Cu Technical future issues:
o
[DISPAA] Technical activities scheduled in the
next six months of the project
o
[All] Open discussion on actions to be carried
out in the next six months of the project.
11:30 [All] Coffee break
12:00 [DISPAA] Management and dissemination
issues
PROJECT LIFE12 ENV/IT/00036
AGENDA
6 MONTH MEETING
•
•
•
•
•
13:00
13:15
13:15
14:00
[All] General questions
End of progress meeting
Tour of CEBASCSIC premises
Lunch
PROJECT LIFE12 ENV/IT/00036
AFTER-CU project will aim at demonstrating
the use of innovative
anti-infective peptide-based molecules
for the environmentally friendly and sustainable
control of bacterial diseases of plants
PLANTS
BACTERIA
disease
DI
Courtesy of E.Colombi, 2012
«The AFTER Cu approach»
A
Courtesy of E.Colombi, 2012
PROJECT LIFE12 ENV/IT/00036
“Disarming” the plant pathogenic bacteria without killing them
using as a target conserved mechanisms for their pathogenicity
Advantages of AFTER Cu strategy
 Low risk of drug resistance emergence
 Active against copper- and antibiotic- resistant bacteria
 Broad spectrum strategy against Gram- plant pathogenic bacteria
 Easily transferable for the control of all Gram- pathogenic bacteria
 Low cost of biotechnological production
 Low environmental impact both in production and application
 ……
PROJECT LIFE12 ENV/IT/00036
Advantages of AFTER Cu strategy
To be demonstrated by

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The design and synthesis of anti-infective peptides
Evaluation of their in vitro and in vivo performances
Evaluation of their ecotoxicological profiles
Model plants used: olive, kiwi and citrus
Challenged with P. savastanoi, P. syringae pv. actinidiae, P.
syringae pv. syringae, respectively
in comparison with effects of traditional copper treatments
PROJECT LIFE12 ENV/IT/00036
PROJECT LIFE12 ENV/IT/00036
PROJECT LIFE12 ENV/IT/00036
Technical Actions carried out
by each partner in the last six months
 B.1: Demonstration of the use of copper compounds for the
control of bacterial diseases of plants important for the EU
 B.2: Demonstration of the qualitative and quantitative yields
of conventional chemical synthesis and biotechnological
synthesis of the anti-virulence peptides at laboratory scale
 B.3: Demonstration of the chemical and biological stability of
anti-virulence peptides produced by conventional chemical
and biotechnological synthesis
.
PROJECT LIFE12 ENV/IT/00036
Technical Actions carried out
by each partner in the last six months
 C.1: Monitoring on the environmental impact of copper
compounds for the crop defence against bacterial
phytopathogens
 C.2: Monitoring of the in vitro anti-virulence performances of
peptides at laboratory level
 C.3: Monitoring of the absence of side effects for the antivirulence peptides on common targets of any living organism at
laboratory level
.
PROJECT LIFE12 ENV/IT/00036
Presentations of:
 DISPAA
 CEBAS
 ICCOMCNR
 ASTRA
 SOLDANO
.
PROJECT LIFE12 ENV/IT/00036
[All] Open discussion on actions carried out
.
PROJECT LIFE12 ENV/IT/00036
PROJECT LIFE12 ENV/IT/00036
Dissemination actions
D1 Demonstration workshop in Italy (last 6 months)
D2 Demonstration workshop in Spain (last 6 months)
D3 Project website (end M3)
D4 Notice boards (end M3)
D5 Layman's report (last 3 months)
D6 Diffusion material preparation
D7 Articles and press releases
D8 Networking
D9 After-Cu technology manual (last 9 months)
D10 Demonstration workshop, seminars, conferences
and other events
D11 Dissemination to Institutions and policy makers
D12 After-LIFE Communication Plan (last 3 months)
D13 International fairs and other events
D14 Digital supports for international diffusion
PROJECT LIFE12 ENV/IT/00036
Dissemination actions carried out
by each partner in the last six months
D3 Project website (end M3)
D4 Notice boards (end M3)
D6 Diffusion material preparation
D7 Articles and press releases
D8 Networking
D10 Demonstration workshop, seminars, conferences
and other events
D11 Dissemination to Institutions and policy makers
D13 International fairs and other events
D14 Digital supports for international diffusion
PROJECT LIFE12 ENV/IT/00036
Dissemination actions carried out
by each partner in the last six months
D3 Project website (end M3)
• After-Cu website in English and in Italian:
www.lifeaftercu.com
• CEBASCSIC is preparing the Spanish version
• Each beneficiary is implementing the link between the
After-Cu website to the beneficiary website
PROJECT LIFE12 ENV/IT/00036
Dissemination actions carried out
by each partner in the last six months
D4 Notice boards (end M3)
• DISPAA produced 10 After-Cu Notice boards and sent
them to all the beneficiaries
• All the beneficiaries put the After-Cu Notice board in a
public space
• All the beneficiaries sent the photos of the After-Cu
Notice board in a public space in each beneficiary
premises
• All the selected beneficiaries will put the After-Cu Notice
board in the demonstration sites
PROJECT LIFE12 ENV/IT/00036
Dissemination actions carried out
by each partner in the last six months
D6 Diffusion material preparation
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2,000 After-Cu brochures
200 After-Cu plastic posters
1 After-Cu tissue poster
1 After-Cu roll-up
1 Italian project presentation
1 Spanish project presentation
200 After-Cu copybooks
200 After-Cu pens
100 After-Cu cups
Decision of the Italian project gadget
PROJECT LIFE12 ENV/IT/00036
Dissemination actions carried out
by each partner in the last six months
D7 Articles and press releases
• 3 articles directly related to After-Cu:
 AGI
 UNIFI website
 TUTTOGREEN (insert of Stampa newspaper)
• 4 indirect articles related to the participation of After-Cu
to the CNR event (in local and web newspaper)
PROJECT LIFE12 ENV/IT/00036
Dissemination actions carried out
by each partner in the last six months
D8 Networking
• Cluster with PODEBA, BIOREM, SOREME, BIONAD,
GREEN SITE, SOILPRO, RESAFE, SEKRET, EVERGREEN (LIFE
10-11-12-13 projects)
• Specific meetings with photos with BIOREM and
CLEANSED
• After-Cu specific presentation at the CNR LIFE event at
Pisa on 25th March 2014
• Networking contacts at the CNR LIFE event at Pisa on 25th
March 2014
PROJECT LIFE12 ENV/IT/00036
Dissemination actions carried out
by each partner in the last six months
D10 Demonstration workshop, seminars, conferences
and other events
• DISPAA partecipated and presented After-Cu at the
LIFE CNR event in CNR premises on 25 March 2014
• University of Siena event about life, Siena 29 May 2014
• 14th International Conference, Na,K-ATPase and
related transport ATPases: Structure, mechanism, cell
biology, health and disease. 30 August - 5 September
2014, The Netherlands
• Seminar in Facultad de Ciencias - Área de Genética of
Universidad de Málaga, Malaga 14 July 2014
• LIFE event in UNIFI in October 2014
PROJECT LIFE12 ENV/IT/00036
Dissemination actions carried out
by each partner in the last six months
D11 Dissemination to Institutions and policy makers
• All the RESAFE beneficiaries are responsible for the
organisation of specific communication activities in
order to disseminate the project actions and results to
Institutions and policy makers.
• Some beneficiaries (ASTRA and CEBASCSIC) started to
have some contacts with Institutions and policy makers
in order to organise specific meetings and events
PROJECT LIFE12 ENV/IT/00036
Dissemination actions carried out
by each partner in the last six months
D13 International fairs and other events
• Erice course on Biophysics of channels and transporters,
Erice 11-17 May 2014
• International Congress on Phytoremediation of Polluted
Soils. Vigo, July 28 – 29, 2014
• 58 Annual Congress of Society of Agricultural Genetics
(SIGA) Quarté Sayàl, Alghero (SS), 15-18 Settembre 2014
• XX National Congress Italian Society for Plant Pathology
(SIPaV) Pisa, 22-23-24 Settembre 2014 "Environmentally
loyal plant protection: from nano- to field-scale“
• Request for ECOMONDO
• Request for EXPO2015
PROJECT LIFE12 ENV/IT/00036
Dissemination actions carried out
by each partner in the last six months
D14 Digital supports for international diffusion
• Each beneficiary started to define the specific video of
each beneficiary activity
• DISPAA started to have some contacts with some expert
video companies in order to organise the general
structure of the video
• DISPAA started to work on the video text
PROJECT LIFE12 ENV/IT/00036
 ACTION E.1: Project management
 ACTION E.2: Monitoring
 DISPAA has continuous contact with all project partners
for monitoring project activities
 DISPAA prepares and sends a summary of the project
activities carried out to monitoring team at the end of
each month
 DISPAA receives from each beneficiary technical inputs
for the monitor month summary
 Definition of management structures
 Definition of payment procedures of each beneficiary
 DISPAA organised the kick-off meeting in January 2014
and the monitoring meeting in June 2014 in DISPAA
premises
 DISPPA defined the Partnership Agreement (following
the EC scheme) and collected all the beneficiaries
signatures
PROJECT LIFE12 ENV/IT/00036
 ACTION E.1: Project management
 ACTION E.2: Monitoring
 DISPAA uses project progress indicators for monitoring
project activities
 DISPAA will revise the project progress indicators in each
of the projects coordination meeting in order to check
any irregularities
 DISPAA will check the number and type of people
reached by each dissemination activity in order to check
if the diffusion actions have met their goals
PROJECT LIFE12 ENV/IT/00036
 ACTION E.1: Project management
 Definition of management structures
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•
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Management and technical committe
DISPAA: Stefania Tegli
ASTRA: Vanni Tisselli
SOLDANO: Maurizio Soldano
CEBASCSIC: Carlos Izquierdo
ICCOMCNR: Emilia Bramanti
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•
•
•
•
•
Administrative committe
DISPAA: Silvia Borselli
ASTRA: Elisabetta Baldassarri
SOLDANO: Domenica Timpano
CEBASCSIC: Maria Teresa Hernandez
ICCOMCNR: Manuela Cempini
PROJECT LIFE12 ENV/IT/00036
 ACTION E.1: Project management
 Definition of payment procedures of each beneficiary
 Each beneficiary has a specific payment responsible
• DISPAA: Stefania Tegli selects the project cost formally
approved by department director or DISPAA council
• ASTRA: Vanni Tisselli as director selects and decides
• SOLDANO: Maurizio Soldano as manager selects and decides
• CEBASCSIC: Carlos Izquierdo selects the project cost formally
approved by department director
• ICCOMCNR: Emilia Bramanti selects the project cost formally
approved by department director
 DISPAA, CEBASCSIC and ICCOMCNR have specific project
code and tender procedures
 ASTRA and Soldano have separated project cost
management and respect best value for money rules
PROJECT LIFE12 ENV/IT/00036
AFTER Cu kick off meeting – 2014, January 10th
Laboratorio Patologia Vegetale Molecolare, DISPAA, Università degli Studi di Firenze,
Polo Scientifico e Tecnologico, Sesto Fiorentino (Firenze)
PROJECT LIFE12 ENV/IT/00036
After-Cu Technical future issues for the next six months
 B.2: Demonstration of the qualitative and quantitative yields of
conventional chemical synthesis and biotechnological synthesis of
the anti-virulence peptides at laboratory scale
 B.3: Demonstration of the chemical and biological stability of antivirulence peptides produced by conventional chemical and
biotechnological synthesis
 B.4: Demonstration of Kilo-scale biotechnological synthesis of the
anti-virulence peptides by recombinant technology
 B.5: Demonstration of the null toxicity profile of the anti-virulence
peptides on model organisms and microorganisms
 B.6 (start): Demonstration of the anti-virulence peptides in vivo
performances at pilot scale level in field screenings
 B.7 (start): Demonstration of the effectiveness of the anti-virulence
peptides in the presence of contamination by copper compounds at
pilot scale level in field screenings
PROJECT LIFE12 ENV/IT/00036
After-Cu Technical future issues for the next six months
 C.2: Monitoring of the in vitro anti-virulence performances of
peptides at laboratory level
 C.3: Monitoring of the absence of side effects for the anti-virulence
peptides on common targets of any living organism at laboratory level
 C.4: Monitoring of the absence of a direct selection operated by the
anti-virulence peptides towards the emergence of bacteria resistant to
the anti-virulence peptides themselves at laboratory level
 C.5 (start): Monitoring of the short term environmental benefits
from the use of the anti-virulence peptides in plant disease control at
pilot scale level in field screenings
 C.6 (start): Monitoring of the absence of an indirect selection
operated by the anti-virulence peptides on copper compound and
antibiotic resistant bacteria at pilot scale level in field screenings
PROJECT LIFE12 ENV/IT/00036
After-Cu Technical future issues for the next six months
[All] Open discussion on actions to be carried out in the next six months
of the project
PROJECT LIFE12 ENV/IT/00036
After-Cu Dissemination future issues for the next six months
D3 Project website: update
 D4 notice boards (end M3): OK
 D6 Diffusion material preparation: OK (foreseen 25 Posters, 10,000
leaflets/brochures/factsheets, 2,500 various branded items, now 2,000
brochures, 200 plastic posters, 1 tissue poster, 1 roll-up, 2
presentations, 500 gadgets)
 D7 Press and media releases: OK (foreseen 30 articles, now 7)
 D8 Networking: OK OK (foreseen 10 projects, now 9)
 D10 Demonstration workshop, seminars, conferences and other events:
organisation and participation in more events (foreseen 4 events, now 4
events)
 D11 Dissemination to Institutions and policy makers: more contacts and
event definition (foreseen successful communication, now contacts)
 D13 International fairs and other events: OK OK (foreseen 4 fairs, now 5
events)
 D14 Digital supports for international diffusion: start (foreseen project
video)
PROJECT LIFE12 ENV/IT/00036
ADMINISTRATIVE ISSUES

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Evaluation of monitoring visit
Inception Report
Next Deliverables
Partner costs June 2014
Next meeting
PROJECT LIFE12 ENV/IT/00036
Evaluation of monitoring visit
EXCELLENT!!!!
No remarks
News: only Inception Report and Final Report
PROJECT LIFE12 ENV/IT/00036
INCEPTION REPORT
DISPAA will be responsible of the production of the Inception report which
will be made up by:
 Progress report with the summary of technical, administrative and
dissemination project issues
 Dissemination Annex
 Deliverables
 Eventual Technical Annexes
 Output indicator tables
 Original Partnership Agreement
 CD with all the documents
The Inception Report will produce the After-Cu GO/NO GO from the EC
The Inception Report deadline is 30 September 2014
All the beneficiaries have to send the technical and administrative
contribution to DISPAA
PROJECT LIFE12 ENV/IT/00036
INCEPTION REPORT
Deadline
 DISPAA will send to each partner a form for technical
and dissemination contribution for the Inception Report
no later than 10th of August
 Each partner has to send to DISPAA all the technical
contribution no later than 10th of September 2014
(except some September activities)
 Each partner has to send to DISPAA all the
dissemination contribution no later than 10st of
September 2014 (except some September activities)
 Each partner responsible for a Deliverable has to send
to DISPAA the Deliverable no later than 10th of
September 2014
 DISPAA will send to EC and Monitoring agency all the
Inception Report documents no later than 30th of
September 2014
PROJECT LIFE12 ENV/IT/00036
NEXT DELIVERABLES
All the following Deliverables will be annexes of the Inception Report :
 Project website: Action D.3
 Notice boards: Action D.4
 Demonstration of the chemical and biological stability of anti-virulence
peptides produced by conventional chemical and biotechnological
synthesis: Action B.3
 Demonstration of the use of copper compounds for the control of
bacterial diseases of plants important for the EU: Action B.1
 Monitoring on the environmental impact of copper compounds for the
crop defence against bacterial phytopathogens: Action C.1
PROJECT LIFE12 ENV/IT/00036
NEXT DELIVERABLES
All the following Deliverables will be annexes of the Inception Report :
 Demonstration of the null toxicity profile of the anti-virulence peptides
on model organism and microorganism: Action B.5
 Demonstration of the qualitative and quantitative yields of conventional
chemical synthesis and biotechnological synthesis of the anti-virulence
peptides at laboratory scale: Action B.2
 Monitoring on the absence of side effects for the anti-virulence
peptides on common targets of any living organism at laboratory level:
Action C.3
 Monitoring on the in vitro anti-virulence performances of peptides at
laboratory level: Action C.2
PROJECT LIFE12 ENV/IT/00036
Partner costs June 2014
PARTNER
CLAIM
Personn Personnel
el
June 2014 Travel
Travel
June
2014
External
External assistanc
Consumab
Overhead
assistan e June Consum les June Overhe s June
ce
2014
ables
2014
ads
2014
DISPAA
205,000
56,720.63 12,500 1,000.00
ASTRA
137,500
9,766.74 12,500 1,000.00
27,800
9,325.64
Soldano
101,500
8,756.43 12,500 1,000.00
52,000
155,000
ICCOMCN
R
CEBAS
88,300 10,000.00 105,000
TOTAL
TOTAL
June 2014
10,750.20 30,000
5,492.96
440,800
83,963.79
45,000
1,017.00 16,000
1,477.66
238,800
22,587.04
22,800 15,000.00
45,000
11,930
1,732.95
193,730
26,489.38
9,253.39 12,500 1,500.00
13,200
25,000
7,000
752.74
109,700
11,506.13
16,490.00 12,500 2,753.27
18,400
95,000 13,227.50 19,000
2,272.95
299,900
34,743.72
TOTAL 651,000 100,987.19 62,500 7,253.27 170,500 34,325.64 315,000
24,994.70 83,930 11,729.26 1,282,930 179,290.06
PROJECT LIFE12 ENV/IT/00036
Project meetings
 1st Kick off meeting in DISPAA in Italy in January 2014
 Monitoring meeting in DISPAA in Italy in June 2014
 2nd Coordination meeting in CEBASCSIC in Murcia (Spain) on July 7th
2014
Next Project meeting
 3rd Coordination meeting in ICCOMCNR in Italy on January 2015