Circuit Training: Water Potential for AP Biology Name: __________KEY______________ Directions: Beginning with the first cell marked #1, determine what biomolecule or subunit is shown. To advance in the circuit, hunt for your answer and mark that cell #2. Continue working in this manner until you complete the circuit. Unless otherwise stated, assume the systems are open and at room temperature (21 degrees celsius). **Disclaimer, the numbers in the problems below have been selected to highlight the problem solving process involved in determining water potential and may not be actual value ranges expected in nature. Ans: -75.155 # __1___ An animal cell is at equilibrium with a beaker of saline solution with a = -1.22. If an animal cell bursts at when its p= 6.5 bars, calculate the molarity of saline solution that the cell must be placed in to make it lyse. Ans: -23.04 # __12___ Because of a cell wall, plant cells have a finite volume that they can achieve. A plant cell at max volume with a s =-7.49 is placed in a solution with s =-0.5. Calculate the resulting p. Ans: -3.0 # __10___ If a plant is droopy and its cells have a p= 0 bars with a s = -5.5 and is placed in a solution of 2M sucrose, what would its resulting be if allowed to reach equilibrium? p= 6.99 = -21.65 Ans: 0.5 # __3___ If the cell from the previous square is placed in a beaker in which = -4.0 bars, in which direction will the net flow of water be? Answer in terms of = -4.0 Ans: -23.48 # ___7__ A cell has a s = -1.2 MPa and p= 0 MPa. If 1 MPa = 10 bars, what is in bars? Ans: -5.83 # _16____ If a cell’s p= 3 bars and = -4.0 bars, calculate it’s s s = -7.0 Ans: -4.0 # __4___ A cell has a s = -3 MPa and p= 1.5 MPa. If 1 MPa = 10 bars, what is in bars? Ans: -21.65 # __11___ If the plant from the previous square was removed from the beaker it was in and placed in a beaker with a 1M sucrose solution, calculate the resulting . = -7.72 = -15 = -12.0 = -23.04 Ans: -7.0 # _17____ What is the s of a 2.5M solution of sodium chloride (salt) at 27 degrees Celsius in an open container? s =124.65 Ans: -4.886 # _14____ A mesophyll cell contains 0.2M glucose. If the cell is at 25 degrees Celsius. If its p= 1 bar, calculate Ans: # __15___ A cell has a s = -0.72 MPa and p= 0.137 MPa, determine its in bars. Ans: 124.65 # __18___ A dialysis tubing of 0.5M NaCl solution is place in a solution of 2.3M NaCl solution at 30 degrees Celsius. Calculate the dialysis tubing’s s. Ans: -12.0 # __8___ The cell from the previous square is placed in a beaker of water with s=-5.0 bars and allowed to reach equilibrium. What will be the resulting ? Ans: 6.99 # __13___ A selectively permeable dialysis tubing containing a 0.7M sucrose solution does not change volume when put in an open beaker of 0.5M glucose solution. What is the dialysis tubing’s p? s= -75.155 Ans: -15 # __5___ If a cell with a solute potential of - 0.25 MPa is placed in a chamber filled with distilled water. A plunger is depressed to create a pressure of 0.4 MPa, which direction will the net flow of water be? Answer in terms of = -0.25 Ans: # __9___ A cell with a = -3.0 bars is placed in a 1.2M NaCl solution at room temperature (21 degrees Celsius), what direction will will the net flow of water be? Answer in terms of = -3.0 Ans: -0.25 # __6___ A cell at 17 degrees Celsius with 0.5M glucose is placed in an open beaker with 0.75M sodium chloride at 12 degrees Celsius. The cell and beaker are allowed to reach equilibrium. What is the cell’s resulting ? = -5.83 = -23.48 = -4.886 Ans: -7.72 # ___2__ If a plant cell’s p= 3 bars and its s = -2.5, what is the resulting = 0.5