KOMP and KOMP2
Trish Labosky
Scientific Co-director
International Project
KOMP
Knock Out Mouse Project
Trans-NIH initiative, aims to generate a
comprehensive and public resource of
murine ESCs containing a null mutation
in every gene in the mouse genome.
KOMP Goals
1) Use gene targeting to generate null alleles,
marked with a high utility reporter, preferably in
C57BL/6;
2) Develop improved C57BL/6 ES cells capable of
robust germline transmission, to be used in a high
throughput pipeline in generating this resource;
1. Regeneron Pharmaceuticals
2. Children's Hospital Oakland
Research Institute (CHORI),
School of Veterinary Medicine,
University of California, Davis
(UC Davis) and the Wellcome
Trust Sanger Institute.
3. The Jackson Laboratory.
3) Support a repository to house the products of this
resource, additional ‘repatriation' effort to bring in
1000 of the existing high priority mouse knockouts;
4) Implement a data coordination center to make
the status and relevant data of the production effort
available to the research community.
www.knockoutmouse.org
Additional KOMP mice
NIH awarded contracts that gave academic
researchers access to a set of wellcharacterized knockout mouse lines from
Deltagen and Lexicon Genetics.
~ $11 million for ~250 lines of these mice
http://www.nih.gov/science/models/mouse/delt
agenlexicon/list.html
http://www.nih.gov/scienc
e/models/mouse/deltagen
lexicon/list.html
Vast majority of mice are from the Wellcome Trust
Nature (2011) 474, 262.
Have targeted more
than 9000 genes.
Conditional allele
Null allele
with reporter
Null allele
What if you don’t know which
genes are involved in your
biological system/question?
KOMP2: Newly Funded
Harwell
Jax
Baylor
UC Davis
Phenotyping Platform
MRRCC
KOMP Davis
Fees
What to do when you find a line of interest:
You submit MTA, order ESCs (we recommend the “PREMIUM ESC PACKAGE”,
$2801), and have them sent to us.
Additional expenses:
ESC expansion, freezeback, prep for path screening
ESC Karyotyping (if needed)
Path screening by DAC
ESC prep / injection day
Injection into Albino BL6 / day
Injection into BL6 / day
$1000 / clone
$350 / clone
$350 / clone
$450
$2600
$1650
TMESCSR Website
http://www.vanderbiltresearch.org/community/profile/id/8