Chapter 9 Energy in a Cell • Cells need energy, its essential to life • Our body has a source of energy available to us • The energy is stored in the chemical bonds of ATP 9.1 ATP in a molecule • ATP = adenosine triphosphate – Adenosine molecule – Three phosphates • ATP is a small compound that is a quick source of energy • Binds to specific sites on a cell • When phosphate bond broken, energy is released – cell uses this energy ADP Adenosine Diphosphate • Formed when a phosphate is cleaved from ATP – Releases energy • ADP can reform ATP which creates a renewable cycle of ATP formation and breakdown • This cycle continues as long as there are phosphate molecules available What do you think would happen if there were no more phosphate molecules available? • ATP binds to a molecule • Phosphate group is cleaved off and ADP leaves – Phosphate group gives the cell its energy • Another ADP molecule binds to the phosphate molecule and the process starts over • Cells use the energy for many purposes – Physical activity – Making new enzymes to carry out chemical reactions – Build membranes and cell organelles – Maintain homeostasis – Dispose of waste substances 9.2 Photosynthesis: Trapping the Sun’s Energy • Photosynthesis • 6CO2 + 6H2O C6H12O6 + 6O2 – Converts light energy into chemical energy – two phases • Light-dependent + light-independent – Builds carbohydrates that store energy – Produces ATP – Occurs in membranes of thylakoid discs • Thylakoid discs – Located in chloroplasts – contain pigments that absorb light = chlorophyll Light-dependent Reactions • First phase of photosynthesis • Sunlight hits plants and they absorb energy • Transfer energy to electrons on the Electron Transport Chain • Electron Transport Chain is a series of proteins embedded in thylakoid membrane • In the ETC, Energy is passed from protein to protein • Each transfer a little bit of energy is released • This energy is used to form ATP from ADP • The ETC also splits a water molecule and hydrogen ions, H+, bind with an NADP+ molecule forming NADPH • NADPH is used to form carbohydrates in light-independent reactions Light-independent Reactions • Calvin Cycle – Takes place in the stroma of the chloroplast – 5 steps – page 235 – Each step adds a CO2 molecule – Takes 6 cycles to form a sugar 9.3 Getting energy to make ATP Cellular Respiration • 3 stages – Glycolysis – Citric acid cycle – Electron transport chain Glycolysis • Anaerobic – no oxygen • Occurs in the cytoplasm • Break down glucose into pyruvic acid • ATP – 2 used, 4 produced; net gain of 2 molecules • Pyruvic acid molecules move to mitochondria, loses a molecule of CO2 and combines with coenzyme A to form acetyl-CoA The Citric Acid Cycle • Glucose is broken down • One ATP molecule formed • Charged electrons are carried via NADH & FADH2 to the electron transport chain in the mitochondria The Electron Transport Chain • Energized electrons passed from protein to protein • Mitochondria inner membrane becomes positive charged, due to high concentration of H+ ions • Mitochondria outside membrane becomes negatively charged due to electrons on the outside • A concentration gradient occurs: electrical & chemical • Electrical = charge difference • Chemical = concentration difference • Inner membrane of mitochondria forms ATP • Final electron acceptor is Oxygen • 32 ATP molecules formed • 35 ATP produced overall • Glycolysis – 2 ATP • Citric Acid Cycle – 1 ATP • Electron Transport Chain – 32 ATP Fermentation Lactic Acid Fermentation • 2 molecules of pyruvic acid use NADH to form 2 molecules of lactic acid • 2 molecules ATP produced • Muscle fatigue = lactic acid build-up Alcoholic Fermentation • Yeast cells produce CO2 and ethyl alcohol • Bread – yeast cells produce carbon dioxide that forms bubbles in dough, heat kills CO2 & bubbles in dough lighten bread Photosynthesis vs. Cellular Respiration • Food accumulated • CO2 taken in • Energy stored in glucose • Occurs only in light • Occurs only in presence of chlorophyll • Food broken down • CO2 given off • Energy released from glucose • Occurs day & night • Occurs in all living cells The End