Lecture 2: Composition of Living Matter CEE 210 ENVIRONMENTAL BIOLOGY FOR ENGINEERS Instructor: L.R. Chevalier Department of Civil and Environmental Engineering Southern Illinois University Carbondale Objectives Investigate the composition of living matter Describe the importance of carbohydrates, lipids, proteins and nucleic acid Explain how DNA and RNA handle biological information Environmental Biology for Engineers Composition of Living Things Organic Compounds Carbohydrates Nucleic acid Lipids Proteins Environmental Biology for Engineers Composition of Living Things Organic Compounds Carbohydrates Nucleic acid Lipids These form the majority of the cell Proteins Environmental Biology for Engineers ___________. They are important for structural material, energy metabolism and other metabolic functions Composition of Living Things Organic Compounds Carbohydrates Nucleic acid Nucleic acid is responsible for Lipids Proteins ______________ Environmental Biology for Engineers Composition of Living Things Carbon - The essential element Carbon (Atomic Number 6) 6p 6n Organic molecules contain carbon backbones. Every carbon atom will form 4 covalent bonds with other atoms, specifically other carbon atoms as well as hydrogen, nitrogen, oxygen and sulfur atoms. Environmental Biology for Engineers By linking together of many smaller molecules, carbon is able to form very large polymers (macromolecules) many of which are important to human physiology. Composition of Living Things Carbohydrates These carbon-based molecules are the major source of energy for the body Carbohydrates are composed of carbon, hydrogen and oxygen in a set proportion ◦ ______________________ Carbohydrates are easily soluble in water due to the polar hydroxyl (OH-) groups Carbohydrates Ingested as sugars and starches Nucleic acid Lipids Proteins Environmental Biology for Engineers Composition of Living Things Three types of carbohydrates _____saccharides are the simplest sugars ◦ Glucose (C6H12O6) is the most abundant (found in fruit) ◦ Also fructose, galactose, ribose _____saccharides are carbohydrates composed of two monosaccharides linked together. ◦ Sucrose is composed of glucose and fructose (table sugar) ◦ Maltose is composed of glucose and glucose chains (found in beer and malt liquor) ◦ Lactose, milk sugar, is composed of glucose and galactose _____saccharides are formed when many monosaccharides link together into long chains ◦ Generally not sweet ◦ Glycogen in animal cells and starch in plant cells are both composed of thousands of glucose molecules linked together. Environmental Biology for Engineers Composition of Living Things Carbohydrate Energy from the bonding of C, H, and O Body uses the carbohydrate to generate glucose Glucose is broken down to produce adenosine triphosphate, or ATP, the fundamental unity of energy Glucose can come from amino acids of protein if carbohydrate supply is low Environmental Biology for Engineers Composition of Living Things Proteins Carbohydrates Nucleic acid Composed of C, O, H and N Uses ◦ ◦ ◦ ◦ Lipids Proteins Source of energy Substrate for tissue growth and maintenance Biological functions Major component of bone and muscle Formation of Proteins ◦ Combination of 20 common amino acids ◦ 10 are essential for humans to obtain from food, since we cannot produce them 8 through life 2 essential during periods of rapid growth (infancy) ◦ Plants can produce all 20 amino acids Environmental Biology for Engineers Composition of Living Things Amino Acids http://www.johnkyrk.com/aminoacid.html Environmental Biology for Engineers Composition of Living Things Lipids Lipids are predominantly composed of hydrogen and carbon atoms linked together by neutral covalent bonds. Carbohydrates Lipids have two distinct regions ◦ Long non-polar (hydrophobic) hydrocarbon chain ◦ Hydrophilic carboxylic acid group Types of lipids ◦ Fat ◦ Steroids ◦ Wax Nucleic acid Lipids Proteins The most important function of lipids is _________________ This membrane facilitates the transport of molecules in and out of the cell Also known in layman’s term as fat ◦ Valuable food source ◦ Each molecule of fatty acid can be converted into twice the number of ATP molecules as glucose Environmental Biology for Engineers Composition of Living Things On-line reference for Lipids Environmental Biology for Engineers http://www.wisc-online.com/objects/ViewObject.aspx?ID=AP13204 Composition of Living Things On-line reference for Carbohydrates Environmental Biology for Engineers http://www.wisc-online.com/Objects/ViewObject.aspx?ID=AP13104 Composition of Living Things On-line reference for Proteins Environmental Biology for Engineers http://www.wisc-online.com/Objects/ViewObject.aspx?ID=AP13304 Composition of Living Things On-line Interactive: Construction of the Cell Membrane This on-line resource shows how some proteins are used in the construction of the cell membrane. Be prepared to answer questions in-class. http://www.wisc-online.com/objects/ViewObject.aspx?ID=AP1101 Environmental Biology for Engineers Composition of Living Things Carbohydrates Nucleic Acid The molecules that carry genetic information Nucleic acid Lipids Proteins ◦ DNA (deoxyribonucleic acid) ◦ RNA (ribonucleic acid) Travel to the tiny world of DNA beginning with the body and ending with the atoms that make up a single DNA base ◦ NOVA: Journey into DNA by Rick Groleau Environmental Biology for Engineers http://www.pbs.org/wgbh/nova/genome/dna.html Composition of Living Things Biological Information Handling http://www.biologyforengineers.org/about.php 2005 Premier Award for Excellence in Engineering Education Courseware by the National Engineering Education Delivery System (NEEDS). Environmental Biology for Engineers Composition of Living Things Additional Resource on Replication of DNA Environmental Biology for Engineers Composition of Living Things Codon Wheel Table AG C U G A C U G A C U G A C U G A C U UC UC U A G C C G U A C U G Environmental Biology for Engineers A G U C AG A C GA CU U G G A CU G A AG A U C A G U C A G U C A G U C A G G U C A G CU A U C Inner circle signifies first nucleotide in codon, second circle signifies second nucleotide in codon, outer circle signifies third nucleotide in codon. Composition of Living Things Objectives Investigate the composition of living matter Describe the importance of carbohydrates, lipids, proteins and nucleic acid Explain how DNA and RNA handle biological information Environmental Biology for Engineers Composition of Living Things References Environmental Biology for Engineers and Scientists (Textbook) ◦ Chapter 3.6 Composition of Living Things Visionlearning ◦ http://www.visionlearning.com/ Chemical Composition of the Body ◦ http://www.biology-online.org/9/1_chemical_composition.htm Amino acid demonstration ◦ http://www.johnkyrk.com/aminoacid.html Cell biology animation ◦ http://www.johnkyrk.com/aminoacid.html Biomolecules: The Lipids, Wisconsin On-Line, Barbara Liang ◦ http://www.wisc-online.com/objects/ViewObject.aspx?ID=AP13204 Biomolecules: The Carbohydrates, Wisconsin On-Line, Barbara Liang ◦ http://www.wisc-online.com/Objects/ViewObject.aspx?ID=AP13104 Environmental Biology for Engineers Composition of Living Things References Biomolecules: The Proteins, Wisconsin On-Line, Barbara Liang ◦ http://www.wisc-online.com/Objects/ViewObject.aspx?ID=AP13304 Construction of the Cell Membrane, Wisconsin On-Line, Barbara Liang ◦ http://www.wisc-online.com/objects/ViewObject.aspx?ID=AP1101 NOVA: Journey into DNA by Rick Groleau ◦ http://www.pbs.org/wgbh/nova/genome/dna.html Biology for Engineers ◦ http://www.biologyforengineers.org/about.php Codon Wheel Table from DNA 2.0 (adapted from) ◦ https://www.dna20.com/index.php?pageID=251 Cellupedia ◦ http://library.thinkquest.org/C004535/introduction.html Environmental Biology for Engineers Composition of Living Things Sources of photographs and images in sidebar Human brain ◦ http://www.healthnak.com/mind/ X-rays images ◦ http://martingallerycharleston.com/index.html Cold Virus (altered in Photoshop) ◦ http://medphoto.wellcome.ac.uk/ Environmental Biology for Engineers Composition of Living Things About the Instructor Professor, Civil and Environmental Engineering Fellow, American Society of Civil Engineers (ASCE) Diplomat, Water Resources Engineering, American Academy of Water Resources Engineering (AAWRE) Board Certified Environmental Engineer, American Academy of Environmental Engineers (AAEE) Licensed Professional Engineer, State of Illinois Environmental Biology for Engineers Composition of Living Things