EP & TA Laboratories and Veterinary
Diagnostics Ltd
16 Phalirou Str. Ayios Pavlos 2362
Nicosia – Cyprus
Tel. No. 00357 22 444 230
Fax No. 00357 22 444 231
Email: eptalab@gmail.com
EP & TA Laboratories ORGANOGRAM
• Dr Pavlos Economides D.V.M.-F.R.S.H
Veterinarian-Bacteriologist – Director
• Dr Savvas Gennaris D.V.M.-Veterinarian
• Dr Marios Christoforou DVM-Msc –
Veterinarian-Associate
• Mr Tefkros Anaxagorou – Quality Manager-
Chief Technician
• Mrs Alkisti Gennari – Chemical Engineer
• Mr Anaxagoras Anaxagorou – Electrical
Engineer-Laboratory Technician
• Mr Anaxagorou Andreas - Sampling
EP & TA Laboratories and Veterinary Diagnostics
Activities
VETERINARY DIAGNOSTICS
-Microbiology
-Parasitology
-Serology
FOOD ANALYSIS
-Bacteriology
-Sensory Testing
-Shelf Life Testing
-Residues of inhibitory substances
• Animal Tissues – Blood samples
• Foods of animal origin
• Meat and meat products
• Milk and milk products
• Fish and fish products
• Animal feeds
• Water
• Water supply – Bacteriological examinations-
Chlorine residues
• Evaluation of working surface Hygiene – Swab tests
• Air samples - Bacteriology
METHODS FOR MICROBIOLOGICAL TESTING OF
FOODS
• Salmonellae ISO 6579-2002-Isolation –
Identification - Serotyping
• Escherichia Coli ISO 21528-2:2004
• Staphylococcus aureus ISO 6888-1:2003
• Listeria monocytogenes - Isolation – Identification
ISO 11290-2:1998
• Colony-count- enumeration of microorganisms in animal feeding stuffs ISO 4833:2003
• Detection of Staphylococcal Enterotoxin in milk products – ELISA –Transia Kit
• Detection and enumeration of Escherichia coli and Coliforms bacteria-Membrane filtration method ISO 9308-1
• Detection and enumeration of intestinal enterococci ISO 7899-2
• Total colony count ISO 6222
• Detection and enumeration of Faecal
Coliforms ISO 9308-1
• Detection of Legionella spp ISO 11731
METHODS FOR MICROBIOLOGICAL
TESTING OF SURFACES AND AIR SAMPLES
SAMPLING
Rodac-contact plates
Hygicult Total Plate Count Agar
&
Hygicult E-Violet Red Bile Agar with addition of glucose
TESTING
• Total Plate Count ISO
4833:2003
• Detection and enumeration of Enterobacteriaceae ISO
21528-2:2004
• Detection of Salmonella spp
ISO 6579-2002
• Detection and Enumeration of Listeria monocytogenes
ISO 11290:1996
• Cultures
• Identification of Bacteria – Biochemical testing
• P.C.R.-Polymerase Chain Reaction
• Immunofluorescence
Polymerase Chain Reaction –
Immunofluorescence Examination
PCR
• Legionella spp
• Salmonellae spp
• Listeria spp
• Rickettsia
• Coxiella
• Leishmania
• Bartonella
Immunofluorescence
• Legionella spp
• Rickettsia
• Coxiella
• Leishmania
• Bartonella
•
•
•
ELISA IN POULTRY
• Infectious Bursal Disease
– IBD
• Infectious Bronchitis Virus
– IBV
• Newcastle Disease Virus –
NDV
• Avian Pneumovirus – TRT
• Reovirus - REO
• Mycoplasma gallisepticum - MG
ELISA IN PIGS
• Reproductive and
Respiratory Syndrome –
PRRS
• Swine Influenza
• Aujeszky Disease
• Mycoplasma
Hyopneumoniae
• Porcine Parvovirus
• Transmissible
Gastroenteritis - TGE
• Corona Virus
•
•
•
• Number of Farms
43
Types of Farms
• Dairy Farms
• Pig Farms
• Poultry Farms
• Rabbit Farms
• Pets-dogs-cats
• Cage birds
• Number of Establishments
78
Types of Establishments
• Poultry Slaughter houses
• Slaughter houses for pigs, sheep and goats
• Food establishments
• Meat Cutting Plants
• Meat Products
• Dairies
• Butcheries
• Hotels
• Restaurants
List of most common Salmonellae serotypes isolated
• Typhimurium
• Braenderup
• Brandenburg
• Newport
• Hadar
• Derby
• Anatum
List of most common Listeria serotypes isolated
• Listeria monocytogenes
• Listeria innocua
• Listeria welshimeri
• Brucellosis
• Rickettsioses
• Coxiella
• Bartonellosis
• Leishmaniosis
• Ehrlichiosis
COLLABORATION WITH OTHER INSTITUTIONS
• World Health Organization – WHO network Salm-
Surv as a General Institution Member for the GSS country Databank
• University of Crete – School of Medicine - Laboratory of Clinical Bacteriology-Parasitology-Zoonoses and
Geographic Medicine
• WHO Collaborating Center for Research and Training in Mediterranean Zoonoses – Anogia - Crete
• Royal Veterinary College London – Department of
Pathology of Infectious Diseases - UK
• We participate in all Interlaboratory testing organized by the National Reference
Laboratory for foods of Animal Origin
July 2004 – Total Colony Count in milk samples
(accuracy d=-0,03)
October 2004 - Total Colony Count in milk samples
(accuracy d=-0,022)
September 2005 – Detection of Salmonella and Listeria in cheese samples (accuracy 100%)
May 2006 - Total Colony Count in milk samples
(accuracy d=-0,009)
EP & TA Laboratories and Veterinary Diagnostics is approved and licensed by
• The Department of Veterinary Services of Cyprus
• The Veterinary Council
The diagnosis tests of animal diseases, zoonotic agents and for the bacteriological examination of foods of animal origin including animal feeds. We are in the process for accreditation in accordance with the requirements of the ISO 17025:2005 standard.