EP & TA Laboratories and Veterinary Diagnostics LTD

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EP & TA Laboratories and Veterinary

Diagnostics Ltd

16 Phalirou Str. Ayios Pavlos 2362

Nicosia – Cyprus

Tel. No. 00357 22 444 230

Fax No. 00357 22 444 231

Email: eptalab@gmail.com

EP & TA Laboratories ORGANOGRAM

• Dr Pavlos Economides D.V.M.-F.R.S.H

Veterinarian-Bacteriologist – Director

• Dr Savvas Gennaris D.V.M.-Veterinarian

• Dr Marios Christoforou DVM-Msc –

Veterinarian-Associate

• Mr Tefkros Anaxagorou – Quality Manager-

Chief Technician

• Mrs Alkisti Gennari – Chemical Engineer

• Mr Anaxagoras Anaxagorou – Electrical

Engineer-Laboratory Technician

• Mr Anaxagorou Andreas - Sampling

EP & TA Laboratories and Veterinary Diagnostics

Activities

VETERINARY DIAGNOSTICS

-Microbiology

-Parasitology

-Serology

FOOD ANALYSIS

-Bacteriology

-Sensory Testing

-Shelf Life Testing

-Residues of inhibitory substances

TYPES OF SAMPLES EXAMINED

• Animal Tissues – Blood samples

• Foods of animal origin

• Meat and meat products

• Milk and milk products

• Fish and fish products

• Animal feeds

• Water

FOOD ESTABLISMENT HYGIENE

• Water supply – Bacteriological examinations-

Chlorine residues

• Evaluation of working surface Hygiene – Swab tests

• Air samples - Bacteriology

METHODS FOR MICROBIOLOGICAL TESTING OF

FOODS

• Salmonellae ISO 6579-2002-Isolation –

Identification - Serotyping

• Escherichia Coli ISO 21528-2:2004

• Staphylococcus aureus ISO 6888-1:2003

• Listeria monocytogenes - Isolation – Identification

ISO 11290-2:1998

• Colony-count- enumeration of microorganisms in animal feeding stuffs ISO 4833:2003

• Detection of Staphylococcal Enterotoxin in milk products – ELISA –Transia Kit

METHODS FOR MICROBIOLOGICAL

EXAMINATIONS OF WATER

• Detection and enumeration of Escherichia coli and Coliforms bacteria-Membrane filtration method ISO 9308-1

• Detection and enumeration of intestinal enterococci ISO 7899-2

• Total colony count ISO 6222

• Detection and enumeration of Faecal

Coliforms ISO 9308-1

• Detection of Legionella spp ISO 11731

METHODS FOR MICROBIOLOGICAL

TESTING OF SURFACES AND AIR SAMPLES

SAMPLING

Rodac-contact plates

Hygicult Total Plate Count Agar

&

Hygicult E-Violet Red Bile Agar with addition of glucose

TESTING

• Total Plate Count ISO

4833:2003

• Detection and enumeration of Enterobacteriaceae ISO

21528-2:2004

• Detection of Salmonella spp

ISO 6579-2002

• Detection and Enumeration of Listeria monocytogenes

ISO 11290:1996

Bacteriological Examination

• Cultures

• Identification of Bacteria – Biochemical testing

• P.C.R.-Polymerase Chain Reaction

• Immunofluorescence

Polymerase Chain Reaction –

Immunofluorescence Examination

PCR

• Legionella spp

• Salmonellae spp

• Listeria spp

• Rickettsia

• Coxiella

• Leishmania

• Bartonella

Immunofluorescence

• Legionella spp

• Rickettsia

• Coxiella

• Leishmania

• Bartonella

SEROLOGICAL TESTING

Standard Agglutination Tests

Complement Fixation Test

ELISA Tests

ELISA TESTS

ELISA IN POULTRY

• Infectious Bursal Disease

– IBD

• Infectious Bronchitis Virus

– IBV

• Newcastle Disease Virus –

NDV

• Avian Pneumovirus – TRT

• Reovirus - REO

• Mycoplasma gallisepticum - MG

ELISA IN PIGS

• Reproductive and

Respiratory Syndrome –

PRRS

• Swine Influenza

• Aujeszky Disease

• Mycoplasma

Hyopneumoniae

• Porcine Parvovirus

• Transmissible

Gastroenteritis - TGE

• Corona Virus

PARASITOLOGICAL TESTING

Microscopical examination

Parasites Egg Count by Floatation –

McMaster

Protozoa

• Number of Farms

43

Types of Farms

• Dairy Farms

• Pig Farms

• Poultry Farms

• Rabbit Farms

• Pets-dogs-cats

• Cage birds

CUSTOMERS

• Number of Establishments

78

Types of Establishments

• Poultry Slaughter houses

• Slaughter houses for pigs, sheep and goats

• Food establishments

• Meat Cutting Plants

• Meat Products

• Dairies

• Butcheries

• Hotels

• Restaurants

SALMONELLAE SEROTYPES

ISOLATED

List of most common Salmonellae serotypes isolated

• Typhimurium

• Braenderup

• Brandenburg

• Newport

• Hadar

• Derby

• Anatum

LISTERIA SEROTYPES ISOLATED

List of most common Listeria serotypes isolated

• Listeria monocytogenes

• Listeria innocua

• Listeria welshimeri

SURVEILLANCE FOR ZOONOSES

• Brucellosis

• Rickettsioses

• Coxiella

• Bartonellosis

• Leishmaniosis

• Ehrlichiosis

COLLABORATION WITH OTHER INSTITUTIONS

• World Health Organization – WHO network Salm-

Surv as a General Institution Member for the GSS country Databank

• University of Crete – School of Medicine - Laboratory of Clinical Bacteriology-Parasitology-Zoonoses and

Geographic Medicine

• WHO Collaborating Center for Research and Training in Mediterranean Zoonoses – Anogia - Crete

• Royal Veterinary College London – Department of

Pathology of Infectious Diseases - UK

PARTICIPATION IN INTERLABORATORY

TESTS

• We participate in all Interlaboratory testing organized by the National Reference

Laboratory for foods of Animal Origin

July 2004 – Total Colony Count in milk samples

(accuracy d=-0,03)

October 2004 - Total Colony Count in milk samples

(accuracy d=-0,022)

September 2005 – Detection of Salmonella and Listeria in cheese samples (accuracy 100%)

May 2006 - Total Colony Count in milk samples

(accuracy d=-0,009)

APPROVAL - LICENCED

EP & TA Laboratories and Veterinary Diagnostics is approved and licensed by

• The Department of Veterinary Services of Cyprus

• The Veterinary Council

The diagnosis tests of animal diseases, zoonotic agents and for the bacteriological examination of foods of animal origin including animal feeds. We are in the process for accreditation in accordance with the requirements of the ISO 17025:2005 standard.

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