AN INTERNSHIP REPORT CARRIED OUT IN SAINT BLAISE CLINIC-MANKON, BAMENDA FROM THE 23RD OF APRIL TO THE 18TH OF MAY 2012 PRESENTED BY: GHANYEN JUDITH NYUYDINKONG ELEVISE ABOKI PIUS SUPERVISED BY: Sr. LILIAN LIMUNGA CHE BMLS (Hons) & Mr. CHEM ELVIS DZELAMONYUY B. Sc (Hons) MICRO BIOLOOGY SUMMARY INTRODUCTION INTERNSHIP AREA CLINIC SETTING LABORATORY AND SPECIMEN FLOW CHART ANALYTICAL RUNS IN THE LABORATORY MEDICAL DIAGNOSIS OF THE MOST PREVALENT DISEASES DISCUSSION RECOMMENDATION AND CONCLUSION CHAPTER ONE DEFINTION OBJECTIVES GEOGRAPHICAL HISTORY LOCATION OF INDEGENES CULTURAL VALUES CHAPTER TWO CLINIC Collection bench Incubator sink V/S Bed Refrigerator F BC machine Spectrophotometer microscope 2 Blood mixer Microscope 1 Electrophoretic machine Central table Serology Sink LABORATORY Parasitology THE SETTING CHAPTER TWO SPECIMEN FLOW CHART LABORATORY STAFF STRENGHT LABORATORY ORGANISATION Reagents Equipment Quality Control ANALYTICAL RUNS PARASITOLOGY: Stool, Urine, PT BIOCHEMISTRY: Electrolytes, ALT, AST, Lipid Profile MICRO BIOLOGY STAINING TECHNIQUES: Gram and Ziehl Neelsen HAEMATOLOGY Hb, WBC differential and total, Blood Sugar, Full Blood count SEROLOGY VDRL, HIV, HBsAg, H. pylori, ASLO, RF, Widal Toxo And Blood Chlamydia Grouping MEDICAL DIAGNOSIS OF THE MOST PREVALENT DISEASE YEAR MP No +VE No –VE 1613 461 MAY 2010-APRIL 2670 2011 1784 MAY2009- APRIL 2010 APRIL 2011-MAY 2012 % +VE TYPHOID % +VE No +ve No-ve 77.8 369 218 62.9 308 89.7 247 198 55.5 857 67.6 217 201 57.9 CHAPTER THREE DISCUSSION, RECOMMENDATION AND CONCLUSION DISCUSSION Work begins with prayers at 7:30am Preparation of work areas in the laboratory from 8:00am Work closes at 3:00pm RECOMMENDATION Stool concentration Urine sedimentation Laboratory manuals Increase staff strength RECOMMENDATION CONTINUES VOLTEX MIXER FOR CHEMISTRY ATLEAST TWO TESTS BE DONE BEFORE DECLARING A PATIENT HIV POSITIVE AND A DONOR NEGATIVE WEIGHT AND BP FOR DONORS STOCK SOLUTION IN LOWER SHELVES A COUNCELLOR FOR PRE AND POST TEST FOR HIV AND HEPATITIS DISTILL WATER FOR REAGENT PREPARATION MONITOR TEMPERATURE OF REFREGERATOR EQUIPMENTS NOT IN USE SHOULD BE KEPT IN THE STORE A COLLECTION TRAY AND LAB FORMS FOR IN PATIENTS CONCLUSION SKILLS ACQUIRED FBC USING AUTOMATED MACHINE COLLECTION OF VENOUS BLOOD USING BUTTERFLY NEEDLE USE OF SPECTROPHOTOMETER USE OF HAEMOBLOBINOMETER SETTING ESR USING PI-PUMP USE OF URINE ANALYZER DIFFIICULTIES Hunger Identification of MP Provision Of Gloves DEDICATION WE DEDICATE THIS PIECE OF WORK TO THE ALMIGHTY GOD FOR THE STRENGHT AND GRACE HE GAVE US THROUGH OUT OUR INTERNSHIP PERIOD ACKNOWLEDGEMENT THE STAFF C.S.H.S THE MATRON OF ST BLAISE CLINIC THE STAFF OF ST BLAISE CLINIC OUR SUPERVISORS PARENTS AND BENEFACTORS CLASSMATES AND FRIENDS SHOOT HIGH FOR THE MOON, EVEN IF YOU MISS YOU WILL FALL BETWEEN THE STARS THANKS FOR LISTENING