Intro to Health Sciences Chapter 20 Laboratory Science in Health Care Lab Science Many forensic investigators who work in CSI start their careers in medical laboratories. Approximately 70-80% of all physician decisions are based on laboratory test results produced by medical laboratory professionals. Laboratory Personnel Do not usually have contact with patients Most of work is done while sitting Requires excellent vision and manual dexterity Environment Clean Well lit Regular hours Frequent use of microscope Areas of Study Clinical labs Blood banking Research and Development Epidemiology Careers Pathologist Laboratory Technologist Medical Laboratory Technician Medical Laboratory Assistant Phlebotomist Careers Life Scientist Animal Scientist Food Scientist Dental Laboratory Technician Environmental Health Technician $15,000-75,000 per year 4 year undergraduate school Careers Pathologist 4 years of medical school 4 years of residency 2 - 4 years of specialty internship Medical Doctor Examines specimens of body tissue, fluids and secretions to: Diagnosis disease Determine effectiveness of treatment Determine cause of death Careers 4 year undergraduate school 1 year internship State Exam for licensure Laboratory Technologist Performs clinical lab testing Analyzes results Cross matches blood for transfusions Calibrates equipment Works under the supervision of the Pathologist Careers Laboratory Technologist Areas of specialization: Microbiology Identifying microorganisms that cause disease Cytotechnologist Analyzing cells for diagnosis Hematology Study of blood Immunology Study of body’s resistance to pathogens Histology Study of tissue Careers Medical Laboratory Technician (MLT) Works under the supervision of the Lab technologist or pathologist Prepares tissue slides Performs simple blood tests 2- 4 years undergraduate school Some states require certification exams for licensure Careers Medical Laboratory Assistant Perform routine tests under the supervision of the technologist Urinalysis, hematology, serology and bacteriology 1 year of education in a hospital or 2 years in a community college or vocational program Certification is possible with examination OJT Careers Phlebotomist Obtain and process blood specimens, throat cultures or wound swabs. or 1 year vocational or community college program Blood Banking Careers Donor recruitment Phlebotomist Blood Bank Lab Supervisor Specialist in Blood Bank Technology (SBB) Blood Banking Career Opportunities Donor Recruitment Organize and plan blood drives Publicity Incentive Programs Donated blood is good for 35 days. Frozen can be kept up to 3 years Blood Banking Career Opportunities Phlebotomist Phlebotomy – venipuncture Tourniquet – used to obstruct blood flow Blood Banking Career Opportunities Lab Supervisor Medical Technologist Blood Banking Career Opportunities Specialist in Blood Bank Technology (SBB) Select donors Draw blood Type blood Run perfusion tests Act as resource in solving problems 4 year undergraduate degree Certification as a MLT Blood Banking Terms Donor – one who gives Recipient – One who receives Career Opportunities Life Scientists Researchers Study living organisms and life processes Many areas of specialization 4 year undergraduate degree 2 year Masters Degree 2 year Doctoral program Career Opportunities Life Scientists Developing new drugs Methods of treatment Developing new plants Environmental protection ¼ work for the federal government 1/3 work for private industry The Rest are in Universities Career Opportunities Life Scientists Areas of specialty: Biology Anatomy Biochemistry Genetics Physics Physiology Microbiology Career Opportunities Life Scientists Microbiologist Virologists Immunologists Biochemists Study bacteria, algae, viruses, and other MO that cause disease. Develop vaccines and new drugs Specialize in viruses Genetics, use of antibodies Reproduction, growth and metabolism Career Opportunities Animal Scientists Study animals to improve feeding, housing, and breeding. Zoologists 4 year undergraduate degree 2 year Masters Degree 2 year Doctoral program Develop new techniques in farming, drugs, pest control Career Opportunities Food Scientists Evaluates safety of food processing Develops new methods of producing foods 4 year undergraduate degree Other Laboratory Personnel Dental Lab Technician Environmental Health Technician Other Laboratory Personnel Dental Lab Technician Do not work directly with patients Make prostheses: Bridges, crowns, inlays, orthodontics. Requires excellent manual dexterity 2 year undergraduate degree Certification is available Other Laboratory Personnel Environmental Health Technician Collect and analyze air and water samples 2-4 year undergraduate degree Laboratory Terms Microbiology Study of microorganisms (microbes) Requires use of microscope Uses microscope to view microscopic organisms? Leeuwenhoek Best Environment for MO Warmth Moisture Food supply Darkness Aerobic - require oxygen Anaerobic - do not require oxygen Terms in Microbiology Non-pathogens Pathogens Carrier Not harmful harmful When an animal carries a MO w/o injury to self. Can still spread the disease Normal Flora Usually live in certain parts of the body w/o causing harm Terms in Microbiology Resident MO that are always present Transient MO that are found temporarily Parasites MO that damage host where they live Terms in Microbiology Infection Incubation Invasion of MO, causing disease Period of time when an infection shows its effects Communicable Able to be transmitted to others Chain of infection The infectious process Chain of Infection Causative agent Microorganism Reservoir Where the agent lives (animal, human, couch, etc) Mode of transmission Mode of entry Susceptible host Direct contact, body secretions, air born, food, water, mosquitoes Break in skin, mucous membrane, any tract When you are sick, malnourished, very old, very young Chain of Infection Susceptible host Causative agent Reservoir Mode of entry Mode of Transmission Means of escape Body’s First Line of Defense The skin Other defense mechanisms: Immune system Vaccinations MO that Cause Disease in Humans Bacteria Fungi Protozoans Metazoans Viruses Rickettsiae MO that Cause Disease in Humans Bacteria Most common Single celled organisms Neither plants or animals Classified according to their shape Rod Round Spiral MO that Cause Disease in Humans Bacteria Anthrax Boils Bronchitis Botulism Gonorrhea Leprosy Pinkeye Pneumonia Tetanus Tonsillitis TB UTI Anthrax Leprosy MO that Cause Disease in Humans Fungi Yeasts and Molds Grow on other organisms: parasitic MO that Cause Disease in Humans Fungi Athlete’s foot Histoplasmosis Ringworm Thrush MO that Cause Disease in Humans Protozoans Animal like One-celled commonly show characteristics usually associated with animals most notably mobility and heterotrophy (consumes food) MO that Cause Disease in Humans Protozoans African Sleeping Sickness Malaria MO that Cause Disease in Humans Metazoans Multi-cellular Worms They can perform apoptosis (type of beneficial cell death) MO that Cause Disease in Humans Metazoans Hookworm Pinworm Tapeworm Trichinosis (eating undercooked pork) MO that Cause Disease in Humans Viruses Not really cells Contains bits of genetic information Reproduce and cause illness inside cell body MO that Cause Disease in Humans Viruses AIDS (HIV) Chickenpox Cold Sores Genital herpes Hepatitis (HBV) Flu Measles Mumps Polio Rabies Warts MO that Cause Disease in Humans Rickettsiae Very small Bacteria-like organisms Cannot live outside living tissue MO that Cause Disease in Humans Rickettsiae Rocky Mountain Spotted fever Epidemiology Study of disease in humans Uses demographic data Helps control and prevent CDC Epidemic – outbreak of disease that effects large number of people in a certain area Terms Agar - usually from algae or seaweed. The word means jelly. Used as a growth medium for microorganisms Petri Dish - a flat dish made of plastic or glass with a cover that is primarily used to grow bacteria. Must be sterilized after use before discarding. Terms Culture and sensitivity – C&S Culture is done to find out what kind of organism is causing an illness or infection. A sensitivity test checks to see what kind of medicine, such as an antibiotic, will work best to treat the illness or infection. Terms Erythrocyte RBC, caries Oxygen Leukocyte WBC, fights infection Type of WBC Phagocyte Thrombocyte Platelet, helps blood to clot Centrifuge Sterile Machine used to separate components of blood No MO Skills Testing Urine for Sugar and Acetone S&A Reagent strip – reacts to chemicals Sugar - glucose Acetone – Ketone, protein byproduct Skills Throat Culture Tongue depressor Culture swab Skills Finger Stick Blood Sugar Autolet - holds lancet Lancet - small needle Glucometer – used to measure blood sugar from a capillary puncture Capillary puncture finger stick Normal: 70-120 Skills Using a Microscope The field of vision becomes smaller as the magnification is increased. Review Lab employee who is a medical doctor specializing in body tissue and fluid analysis? Pathologist Review A laboratory technician requires __________ years of education ? 4 years plus 1 year internship Review Educational training for a MLT ? 2-4 years Review Draws blood, can be educated with OJT? Phlebotomist Review 4 year degree? Bachelors degree Review Study of body tissues? Histology Review Study of blood? hematology Review One who receives a donation form another? recipient Review Who invented the compound microscope? A. Janassen B. Fleming C. Leeuwenhook D. Lannec Review MO prefer an environment that is ? A. warm B. dark C. dry Review Harmful MO ? pathogens Review Bacteria that is not harmful when it is found in a certain location ? Normal flora. Review Washing your hands breaks what chain in the chain of infection ? Causative agent Review Wearing gloves breaks what chain in the chain of infection ? Portal of entry Review Getting 8 hours of sleep each night and eating healthy each day breaks what chain in the chain of infection ? Susceptible host Review An invasion of MO, resulting in disease ? infection Review Vaccinations are the body’s first line of defense. True False Review Natural acquired immunity occurs as a result of having the disease. True or False Review bacteria Review protozoan Review TB, UTI, Pinkeye ? bacteria Review Hepatitis, Aids, flu ? Virus Review Tapeworm, pinworm? Metazoan Review Athlete’s foot, ringworm? fungus Review Rocky Mountain Spotted Fever? Rickettsiae Review Outbreak of disease in a certain location ? epidemic Review HCT ? Hematocrit H&H ? Hemoglobin and Hematocrit Review HIV ? Human immunodeficiency virus HBV ? Hepatitis B Review CDC ? Center for Disease Control Review glucometer Review lancets Review What is the most important way to prevent the spread of microorganisms? Hand washing Questions??