CHAPTER 7: CELL STRUCTURE AND FUNCTION Chapter 7 – The Cell – IQ # 1 1. What century did the use of the microscope begin (p. 169)? 2. What does cell theory state? Summarize in your own words (p. 170). 3. Define Prokaryote and Eukaryote, what are the example organisms that are associated with each type (p. 173)? 7-1 Life Is Cellular A. The Discovery of the Cell 1. Early Microscopes • Robert Hooke- Used compound microscope to look at a slice of cork • Anton van Leeuwenhoek- Observed tiny living things in pond water 2. The Cell Theory • Mathias Schleiden- Concluded all plants are made of cells Stated all animals are made of cells Concluded new cells come from • Rudolf Virchow- existing cells • Theodor Schwann- Cell Theory: cells • All living things are composed of _____ • Cells are the basic units of structure and function in living things Existing cells • New cells are produced from ____________ Where does the mold come from? Exploring the Cell B. Exploring the Cell 1. Electron Microscope (TEM & SEM) -Specimen placed in a vacuum http://www.mos.org/sln/sem/ Scanning Electron Microscope Mosquito Head Old, Used Mascara Brush Exploring the Cell B. Exploring the Cell 1. Electron Microscope (TEM & SEM) -Specimen placed in a vacuum http://www.mos.org/sln/sem/ 2. Scanning Probe Microscope -1990 development of fine probe microscope ordinary air -operates in _______________ -can even show samples in solution Microscopes • The light microscope enables us to see the overall shape and structure of a cell Image seen by viewer Eyepiece Ocular lens Objective lens Specimen Condenser lens Light source Figure 4.1A C. Prokaryotes and Eukaryotes 0.2 micrometers • cells vary in size from _________________1000 micrometers ___________________ • Viruses are not cells Prokaryotic cell Eukaryotic cell Cell membrane Cytoplasm Cell membrane Organelles Cytoplasm Nucleus Eukaryotic Cell vs. Prokaryotic cell Prokaryotes pro=__________ before karyote= kernel (nucleus) generally smaller less complicated Single Celled no Membrane bound organelles Kingdom • Monera - Eubacteria (Bacteria) Common to Both Eukaryotes Bacteria Cells Prokaryotes Pro= before karyote= kernel (nucleus) generally smaller less complicated Single Celled no Membrane Bound Organelles Kingdom • Monera - Eubacteria (Bacteria) Archaea (catch all for single celled organisms) Common to Both Eukaryotes Archaea Prokaryotes pro=__________ before karyote= kernel (nucleus) generally smaller less complicated Single Celled no Membrane bound organelles Kingdom • Monera - Eubacteria (Bacteria) Archaea (catch all for single celled organisms) Common to Both contain DNA __________ Cell membrane Ribosomes ___________ Eukaryotes true Eu= ___________ Karyote=kernel (nucleus) Generally larger Contain membrane bound organelles (“little organs”) Ex: Plants, animals, fungi, and protists Fungi Protists Eukaryotic Cell vs. Prokaryotic cell Prokaryotic vs. Eukaryotic Cells Prokaryotes Cell membrane Contain DNA Ribosomes Eukaryotes Nucleus Organelles: Endoplasmic reticulum Golgi apparatus Lysosomes Vacuoles Mitochondria Cytoskeleton Monday Night Homework • Happy Halloween!! IQ # 2 – 7.1 Review • How do prokaryotes and eukaryotes differ? How are they the same? • What are the three parts of Cell Theory? • Matching 1. 2. 3. 4. 5. 6. Plants Archaea Animals Fungi Bacteria Protists A. Prokaryotic B. Eukaryotic Announcements • Make-Up Day – TOMORROW – Wednesday Nov 2 – Lunch – Quizzes • Josh H – Quiz 6 • Ryan C – Quiz 2 – After School or 6th Period – Exams • Breanna R – Test # 4 • Extra Credit – TOMORROW – Wednesday Nov 2 – Work on Sections 7.1-7.2 in Workbook Microscope Lab Microscopes Image seen by viewer Eyepiece Ocular lens Objective lens Specimen Condenser lens Light source Tuesday Night Homework • Due November 2 – Section Assessment 7.1 • Pg. 173 • Questions 1-5 IQ # 3 1. What differences between Plant and Animal cells do you see (p. 175)? 2. What is the structure of the nucleus? What is held inside the nucleus (P. 176)? 3. What do Ribosomes do? Where can you find Ribosomes (p. 177)? 4. What does the Endoplasmic Reticulum do? How many types of E.R. are there? How do they differ in function (p. 177-178)? 5. What is the structure of the Golgi Apparatus? What are its functions (p. 178)? Agenda • Review Prokaryotes v. Eukaryotes – Khan Academy Video ~ 15 Minutes – Bacteria – Intro to Bacteria • Begin Cell Organelles Lecture – Nucleus – Ribosomes – E.R. – Golgi Apparatus CA State Science Standards 1. The fundamental life processes of plants and animals depend on a variety of chemical reactions that occur in specialized areas of the organism’s cells. As a basis for understanding this concept: E. Students know the role of the endoplasmic reticulum and Golgi apparatus in the secretion of proteins. F. Students know usable energy is captured from sunlight by chloroplasts and is stored through the synthesis of sugar from carbon dioxide. G. Students know the role of the mitochondria in making stored chemical-bond energy available to cells by completing the breakdown of glucose to carbon dioxide. Bacteria – An Introduction • Khan Academy • http://www.khanacademy.org/video/bacteria ?playlist=Biology • HANDOUT Nucleus • “Control Center for the Cell” • Contains DNA • Nuclear Envelope – 2 membranes – Pores • Chromatin or Chromosomes • Nucleolus – Ribosome Synthesis NUCLEUS Chromatin Nucleolus Two membranes of nuclear envelope Pore ROUGH ENDOPLASMIC RETICULUM Ribosomes • • • • • Ribosomes “Producer of the Proteins” Small pieces of RNA Found throughout the cytoplasm 2 subunits Free Ribosomes – In Cystol (Cytoplasm) – For use in the Cell • Bound Ribosomes – In Rough Endoplasmic Reticulum (ER) – For use in membranes, organelles, or export Endoplasmic Reticulum - E.R. • • • • “Maker of Exports” Lipids for Cell Membrane created Proteins and Other Materials Exported Smooth E.R. – Contain enzymes – Detoxification (Lots of Smooth E.R. in Liver Cells) • Rough E.R. – Rough part is Ribosomes on surface of E.R. – Portion involved in protein synthesis SMOOTH ER ROUGH ER Nuclear envelope Ribosomes SMOOTH ER Figure 4.9 ROUGH ER Golgi Apparatus • “Exporter” • Stacks of sacks • Golgi modifys, sorts, packages proteins (and other material) from the E.R. for – Storage – Secretion • In high density in cells that secrete a lot – Salivary Glands – Pancreas Wenesday Night Homework • Due November – Section Assessment 7.2 • Pg. 181 • Questions 1-5 IQ # 4 1. Why does the nucleus have pores? What is the function of Nucleolus? What does DNA look like if it is in the chromatin state (Draw it)? 2. What does a Ribosome look like (DRAW IT)? What is its main function? What is it made of? 3. Make a chart What are the 2 types of E.R.? How do their functions differ? How do they look different (Draw it)? 4. What does the Golgi Apparatus look like (Draw It!)? What are its three main functions? What organelle do its materials come from and where are they headed? (Draw the chain of events) Announcements • Tomorrow is Friday What does that mean? QUIZ!!! • Sections 7.1-7.2 – Organelles – Difference between Prokaryotes and Eukaryotes – Difference in plant and animal cells Agenda • IQ # 4 • Lecture Cell Oranelles, Part 2 – Lysosomes – Vacuoles – Mitochondria – Chloroplasts – Cytoskeleton • Cell City Analogy Activity • Review of 7.1 and 7.2 Homework Lysosomes • “The Clean-Up Crew” • Organelles filled with Enzymes – Bud off of Golgi • Digestion of lipids, carbohydrates, and proteins into components • Breakdown of old, dead organelles – Keep cell healthy • Found in high densities in White Blood Cells • Lysosomes Fail - – Tay-Sac Disease LYSOSOME Nucleus Rough ER Transport vesicle (containing inactive hydrolytic enzymes) Plasma membrane Golgi apparatus Engulfment of particle Lysosome engulfing damaged organelle “Food” LYSOSOMES Food vacuole Digestion Vacuoles • “The Storage Unit” • Storage of – – – – Water Salts Proteins carbohydrates • Typically very large in plant cells – Create enough pressure so cells can structurally support heavy items • Some animals can actively pump water in and out • Central in the cell Mitochondria and Chloroplasts • “The power company” • Energy harvesting Organelle • Chloroplasts – Only photosynthetic organisms • Plants • Photosynthetic Bacteria/ Algae • Mitochondria – ALL living cells Mitochondria • “The Converter” • Convert chemical energy from food into usable energy in the cell • 2 Membranes • Has own DNA and Ribosomes – Acts mostly independently from cell • Location for Cellular Respiration ATP Production • All your mitochondria are from your Mother! MITOCHONDRION Outer membrane Intermembrane space Inner membrane Cristae Figure 4.16 Matrix Chloroplasts • “The Light Catcher” • Capture sunlight and convert it into chemical energy Photosynthesis (Glucose Production) • 2 Membranes • Has own DNA Chloroplast Stroma Inner and outer membranes Granum Intermembrane space Cytoskeleton • “The Steel of the Cell” • Network of protein filaments – Maintain shape in the cell – Allow the cell to move • Microfilaments – – – – Thread like Made of Actin Extensive, forms tough flexible framework Aid in movement (like an amoeba crawling) • Microtubules – – – – Hollow Made of Tubulin Important in cell division (spindle fibers) Flagella or Cilia The Cytoskeleton Actin subunit Tubulin subunit Fibrous subunits 25 nm 7 nm MICROFILAMENT Figure 4.17B 10 nm INTERMEDIATE FILAMENT MICROTUBULE Cell Wall • • • • “The Armory” Only found in Plant Cells Adds strength to the cell Keeps water pressure from exploding the cell Cell Membrane • Selectively Permeable • 7.3 – Whole section on it. 7-2 Eukaryotic Cell Structure(chart) Prokaryotes Eukaryotes Section 7-2 Venn Diagrams Cell membrane Contain DNA ribosomes Animal Cells Lysosomes Plant Cells Cell membrane Ribosomes Nucleus Endoplasmic reticulum Golgi apparatus Vacuoles Mitochondria Cytoskeleton Cell Wall Chloroplasts Central Vacuole Nucleus Endoplasmic reticulum Golgi apparatus Lysosomes Vacuoles Mitochondria Cytoskeleton Nucleus Rough endoplasmic reticulum Plant Cell Ribosomes Smooth endoplasmic reticulum Golgi apparatus Microtubule Not in animal cells Central vacuole Intermediate filament Chloroplast Microfilament Cell wall Mitochondrion Peroxisome Plasma membrane Figure 4.5B Cytoskeleton Animal Cell Smooth endoplasmic reticulum Nucleus Rough endoplasmic reticulum Flagellum Not in most plant cells Lysosome Centriole Ribosomes Peroxisome Microtubule Cytoskeleton Intermediate filament Microfilament Figure 4.5A Golgi apparatus Plasma membrane Mitochondrion Animal Cell Figure 4.5A Plant Cell Figure 4.5B Chapter 7 – The Cell – IQ # 1 1. What century did the use of the microscope begin (p. 169)? 2. What does cell theory state? Summarize in your own words (p. 170). 3. Define Prokaryote and Eukaryote, what are the example organisms that are associated with each type (p. 173)? IQ # 2 – 7.1 Review • How do prokaryotes and eukaryotes differ? How are they the same? • What are the three parts of Cell Theory? • Matching 1. 2. 3. 4. 5. 6. Plants Archaea Animals Fungi Bacteria Protists A. Prokaryotic B. Eukaryotic IQ # 3 1. What differences between Plant and Animal cells do you see (p. 175)? 2. What is the structure of the nucleus? What is held inside the nucleus (P. 176)? 3. What do Ribosomes do? Where can you find Ribosomes (p. 177)? 4. What does the Endoplasmic Reticulum do? How many types of E.R. are there? How do they differ in function (p. 177-178)? 5. What is the structure of the Golgi Apparatus? What are its functions (p. 178)? IQ # 4 1. Why does the nucleus have pores? What is the function of Nucleolus? What does DNA look like if it is in the chromatin state (Draw it)? 2. What does a Ribosome look like (DRAW IT)? What is its main function? What is it made of? 3. Make a chart What are the 2 types of E.R.? How do their functions differ? How do they look different (Draw it)? 4. What does the Golgi Apparatus look like (Draw It!)? What are its three main functions? What organelle do its materials come from and where are they headed? (Draw the chain of events) 7-2 Eukaryotic Cell Structure(chart) Prokaryotes Eukaryotes Section 7-2 Venn Diagrams Cell membrane Contain DNA ribosomes Animal Cells Lysosomes Plant Cells Cell membrane Ribosomes Nucleus Endoplasmic reticulum Golgi apparatus Vacuoles Mitochondria Cytoskeleton Cell Wall Chloroplasts Central Vacuole Nucleus Endoplasmic reticulum Golgi apparatus Lysosomes Vacuoles Mitochondria Cytoskeleton Thursday Night Homework • Due November 4 – Cell City Analogy • Complete the example given • Create your own analogy • Draw your own analogy! – Be creative – QUIZ TOMORROW!!! IQ # 5 – 7.3 1. What does the cell membrane do? What is it composed of? What is the difference between Cell Membrane and the Cell Wall? (p. 182-183) 2. What is diffusion? When does it stop? Does diffusion require energy? (p. 184) 3. What is Osmosis? How does it differ from diffusion? What are the three types of osmotic situation? (p. 185) 4. Define Facilitated Diffusion. Does it require energy? What object in the cell membrane assists in facilitated diffusion? Announcements • Collect last weeks extra credit • Tomorrow – Troy Central Tutoring – Sections 7.3-7.4 • Grades are submitted, on ABI • Test – Chapter 7 – Tuesday – November 15th – Tentatively Agenda • Review Quiz • Go over Yesterdays Lab • Lecture 7.3 – Cell Membranes – Diffusion – Osmosis 7-3 Cell Boundaries A. Cell Membrane leaves the cell • Regulates what ___________ and __________ enters • Provides protection and support • Composed of a ______________ lipid bilayer CELL MEMBRANE “FLUID MOSAIC” Outside of cell Carbohydrate chains Proteins Cell membrane Inside of cell (cytoplasm) Protein channel Lipid bilayer “Phospholipids” 7-3 Cell Boundaries A. Cell Membrane leaves the cell • Regulates what ___________ and __________ enters • Provides protection and support • Composed of a ______________ lipid bilayer Protein molecules are embedded in the bilayer; • __________________ form channels and pumps to move material across the cell membrane. CELL MEMBRANE “FLUID MOSAIC” Outside of cell Carbohydrate chains Proteins Cell membrane Inside of cell (cytoplasm) Protein channel “transport” Lipid bilayer “Phospholipids” 7-3 Cell Boundaries A. Cell Membrane leaves the cell • Regulates what ___________ and __________ enters • Provides protection and support • Composed of a ______________ lipid bilayer Protein molecules are embedded in the bilayer; • __________________ form channels and pumps to move material across the cell membrane. Carbohydrates are attached to some of the proteins; • _________________ allows cells to __________ identify one another. CELL MEMBRANE “FLUID MOSAIC” Outside of cell Carbohydrate chains Proteins “ID tags” Cell membrane Inside of cell (cytoplasm) Protein channel “transport” Lipid bilayer “Phospholipids” Function of the Plasma Membrane **Small, Nonpolar molecules easily pass through the membrane: O2, CO2, Hormones, Steroids vs. **Large, Charged do not pass through easily and must be helped in. These molecules would include: C6H12O6 , Proteins, and Ions B. Cell Walls • Found in plants, algae, fungi and many prokaryotes • Cell walls are porous enough to allow water, oxygen, carbon dioxide easily • Main function is to provide support and protection for the cell cellulose • Plant cell walls made of __________ (carbohydrate fiber) C. Diffusion Through Cell Boundaries •All living cells exists in a liquid environment. •Cell membranes regulate the movement of molecules in and out of the cell. selectively permeable •Cell membranes are _____________________ semipermeable (aka: _______________) •If substances can pass, then the cell membrane = Permeable •If substances cannot pass, then the cell membrane= impermeable 1. Measuring Concentration • Cytoplasm= Water and other substances between the cell membrane and the nucleus. • Concentration= mass of solute/volume of solution 4 g/L • Example:12g salt/3L H2O= 60g salt/3L H2O= 20 g/L 5X more concentrated • Concentration gradient= unequal distribution of particles IQ # 6 1. What molecules can move easily through your cell membrane? Which molecules cannot? (review lecture notes from yesterday) 2. Draw the lipid bilayer (cell membrane) including the phospholipids, proteins, and carbohydrates. (p. 182). 3. Describe Figure 7-15. What is happening? What is moving across the membrane. (p. 185) 4. What is the difference between hypertonic, hypotonic, and isotonic? (p. 185) Announcements • Today – Extra Credit – Sections 7.3-7.4 – Pass back old EC worksheets • Tomorrow – Quiz • Next Tuesday – Exam! Agenda • Diffusion Review – Demonstration • Osmosis – Starch/ Glucose Demonstration 2. Diffusion collide and spread out randomly in • Particles constantly solution high concentration _____ to a ____ low concentration • Particles move from a _____ (with the concentration gradient); process is known as _________ diffusion • Diffusion continues until equilibrium is reached • Equilibrium = When particles are evenly distributed in solution • Diffusion depends upon random particle movements, substances energy diffuse across membranes without ______ requiring the cell to use _______ • The movement of particles will continue to move equally across the cell membrane to maintain equilibrium. D. Osmosis • H2O passes easily across most membranes • Osmosis = The diffusion of water through a selectively permeable membrane Before Osmosis Selectively permeable membrane After Osmosis water solute Figure 7-15 Osmosis 1. How Osmosis Works Section 7-3 Left Beaker: left side (Highly concentrated) • More sugar molecules on the ____ • Low concentration on the right side. • The membrane is permeable to water but not sugar. • Net movement of water from low concentration to high concentration. TYPES OF SOLUTIONS Hypertonic Solution “above strength” High concentration of Dissolved substances In Solution Isotonic Solution “same strength” Hypotonic Solution “below strength” Equal concentration of dissolved substances Low concentration of dissolved substances in solution Cells in a hypertonic solution In a hypertonic solution, water leaves a cell by osmosis, causing the cell to shrink. H2O H2O Water Molecule Dissolved Molecule Cells in a hypotonic solution In a hypotonic solution, water enters a cell by osmosis, causing the cell to swell. H2O H2O Water Molecule Dissolved Molecule Cells in an isotonic solution In an isotonic solution, the concentration of dissolved substances in the solution is the same as the concentration of dissolved substances inside the cell. H2O H2O Water Molecule Dissolved Molecule Isotonic Hypotonic Hypertonic 2. The effects of Osmosis on cells Cells in a hypertonic solution Cells in an isotonic solution Cells in a hypotonic solution E. Facilitated Diffusion • Molecules, that cannot diffuse across the cell membrane’s lipid bilayer on their own, can move With the help of protein channels Facilitated Diffusion • Molecules stillmove ___________________ from high to low concentration Section 7-3 Glucose molecules High Concentration Cell Membrane Low Concentration Protein channel F. Active Transport • Movement of molecules against a concentration gradient (from low to high) • Requires energy • Needs a transport protein or pump Energy Molecule being carried Molecule to be carried Large molecules and clumps of material can be taken into the cell by a process known as ___________. endocytosis The two kinds of endocytosis are: 1. Phagocytosis= ‘Cell eating’ 2. Pinocytosis= ‘cell drinking’ Exocytosis release of large amounts of material ___________= Announcements • Collect Extra Credit • Exam on Wednesday • Tomorrow: – Play awesome review game Agenda • • • • Finish “Secrets of the Cell” Go over Quiz Finish Ch. 7 Lecture Review Old HWs and IQs Transport of Large Particles • Endocytosis is a process by which a cell surrounds and takes in material from its environment. Nucleus Digestion Exocytosis Endocytosis Wastes removal 1. interactive sites for cell membrane, diffusion, active, passive transport Let’s Review: PASSIVE TRANSPORT 1.Simple diffusion (includes Osmosis) * No ATP Hi-Lo No protein ACTIVE TRANSPORT 2. Facilitated Diffusion * No ATP * ATP required Hi-Lo Lo-Hi Needs protein Needs Protein 7-4 The Diversity of Cellular Life A. Unicellular Organisms (single celled) outnumbermulticellular • Unicellular organisms _________ organisms • Examples: Yeast, algae, bacteria B. Multicellular Organisms (many celled) specialized to perform different tasks • Cells become ___________ • Cells need to communicate and cooperate C. Levels of Organization • The levels of organization in a multicellular organism are: individual CELLS TISSUES ORGANS Section Muscle cell Smooth muscle ( ti ssue) Stomach (organ) ORGAN SYSTEMS Digesti ve system (organ system) 1. Tissues= Group of similar cells that perform a particular function • Four types of tissue: - muscle - epithelial - nervous - connective 2. Organs= Groups of tissues • Ex. bicep muscle is made of muscle, connective, and nervous tissue 3. Organ Systems= Group of organs that work together to perform a specific function. Homework • Ch. 7 Review Sheet Chapter 7 – The Cell – IQ # 1 1. What century did the use of the microscope begin (p. 169)? 2. What does cell theory state? Summarize in your own words (p. 170). 3. Define Prokaryote and Eukaryote, what are the example organisms that are associated with each type (p. 173)? IQ # 2 – 7.1 Review • How do prokaryotes and eukaryotes differ? How are they the same? • What are the three parts of Cell Theory? • Matching 1. 2. 3. 4. 5. 6. Plants Archaea Animals Fungi Bacteria Protists A. Prokaryotic B. Eukaryotic IQ # 3 1. What differences between Plant and Animal cells do you see (p. 175)? 2. What is the structure of the nucleus? What is held inside the nucleus (P. 176)? 3. What do Ribosomes do? Where can you find Ribosomes (p. 177)? 4. What does the Endoplasmic Reticulum do? How many types of E.R. are there? How do they differ in function (p. 177-178)? 5. What is the structure of the Golgi Apparatus? What are its functions (p. 178)? IQ # 4 1. Why does the nucleus have pores? What is the function of Nucleolus? What does DNA look like if it is in the chromatin state (Draw it)? 2. What does a Ribosome look like (DRAW IT)? What is its main function? What is it made of? 3. Make a chart What are the 2 types of E.R.? How do their functions differ? How do they look different (Draw it)? 4. What does the Golgi Apparatus look like (Draw It!)? What are its three main functions? What organelle do its materials come from and where are they headed? (Draw the chain of events) IQ # 5 – 7.3 1. What does the cell membrane do? What is it composed of? What is the difference between Cell Membrane and the Cell Wall? (p. 182-183) 2. What is diffusion? When does it stop? Does diffusion require energy? (p. 184) 3. What is Osmosis? How does it differ from diffusion? What are the three types of osmotic situation? (p. 185) 4. Define Facilitated Diffusion. Does it require energy? What object in the cell membrane assists in facilitated diffusion? IQ # 6 1. What molecules can move easily through your cell membrane? Which molecules cannot? (review lecture notes from yesterday) 2. Draw the lipid bilayer (cell membrane) including the phospholipids, proteins, and carbohydrates. (p. 182). 3. Describe Figure 7-15. What is happening? What is moving across the membrane. (p. 185) 4. What is the difference between hypertonic, hypotonic, and isotonic? (p. 185) IQ # 7 – Section 7.4 1. What is the difference between unicellular organisms and multicellular organisms? (p. 190) 2. Describe Cell Specialization. What are 3 examples of specialized animal cells? What are 2 examples of specialized plant cells? (p. 191-192) 3. What does a histotechnologist do? (p. 192) 4. What are the three levels of organization discussed? Name an example for each level. (p. 192-193)