SUPPLEMENTARY MATERIAL
Appendix 1. Sources and purities of volatile compounds used in electrophysical experiment
with Atrichelaphinis tigrina. Superscript numbers denote the order in which compounds were
puffed onto antennae (Paraffin and air were puffed before and after each sequence.
Benzaldehyde was puffed first and after every 5 compounds).
Compound
Purity (%)
CAS number
Product code and Source
≥99%
100-66-3
10520, Fluka
Benzaldehyde1
≥99%
100-52-7
B1334, Sigma-Aldrich
Benzyl alcohol7
≥99%
100-51-6
B16208, Aldrich
≥90%
(E) 37526-88-8
W33318 Aldrich
~97%
28127-58-4
340510, Aldrich
Hexyl tiglate13
≥97%
16930-96-4
W500909, Sigma-Aldrich
(R)-(+)-Limonene15
≥97%
5989-27-5
183164, Sigma
ß-Linalool5
≥95%
78-70-6
62140, Fluka
(E/Z)-Linalool oxide
≥97%
60047-17-8
62141, Aldrich
Methyl benzoate9
≥99.5%
93-58-3
18344, Fluka
Methyl salicylate6
Anisole
2
Benzyl tiglate12
sec-Butyl tiglate
11
(furanoid)4
≥99%
119-36-8
M6752, Sigma-Aldrich
14
~90%
123-35-3
70049, Fluka
Ocimene10
≥90%
13877-91-3
W353901, mix of isomers, Aldrich
BV406
Alpha Pharm
≥98%
60-12-8
P6134 Sigma
≥99%
18172-67-3
80608, Fluka
Myrcene
Paraffin
2-Phenylethyl alcohol8
ß-Pinene
3
Appendix 2. The color spectral reflectance for various inflorescence parts and leaves of
Protea caffra, and green and yellow bucket traps used for field trapping experiments.
Appendix 3. Total number of insects belonging to different taxonomic groups caught in
bucket traps used to test the scent preference of insects in the field to five test compounds
compared to paraffin controls.
Insect order
Test compound
Benzaldehyde
Linalool
Linalool
Methyl
Methyl
oxide
benzoate
salicylate
Paraffin
(furanoids)
Coleoptera (total)
101
138
112
151
64
34
—Atrichelaphinis
64
99
73
122
33
19
Dictyoptera
-
1
-
-
-
-
Diptera
-
-
3
1
4
4
Hemiptera
-
1
-
2
2
-
Hymenoptera
-
-
1
4
-
1
tigrina
Lepidoptera
2
6
-
1
3
Appendix 4. The relationship between emission rates and vapor pressure for 2 ml solutions of
five test compounds (1:19 test compound: paraffin). BA = benzaldehyde, Lin = linalool,
LinOx = linalool oxide, MeBen = methyl benzoate, MeSal = methyl salicylate. Vapor
pressures were taken from the SRC PhysProp Database
(http://www.syrres.com/esc/physdemo.htm). (Regression: y = 1.71x + 101.92, R2 = 0.97, P <
0.01).
3
Appendix 5. The relationship between mean EAG response of Atrichelaphinis tigrina and
vapor pressure for fifteen test compounds. Ani=anisole, BenOH=benzyl alcohol,
BenTig=benzyl tiglate, ButTig=butyl tiglate, BA = benzaldehyde, HexTig=hexyl tiglate,
Lim=(R)-(+)-limonene, Lin = linalool, LinOx = linalool oxide, MeBen = methyl benzoate,
MeSal = methyl salicylate, Myr=myrcene, Oci=Ocimene, PhenEth=2-phenylethyl alcohol,
Pin=pinene. Vapour pressures were taken from the SRC PhysProp Database
(http://www.syrres.com/esc/physdemo.htm) and the free chemical database, ChemSpider
(http://www.chemspider.com). (Regression: y = 1.792x + 0.2399, R2 = 0.185, P = 0.11)