Papers presented at Journal Club The broadly conserved regulator PhoP links pathogen virulence and membrane potential in Escherichia coli. Alteri CJ, Lindner JR, Reiss DJ, Smith SN, Mobley HL. Transfer-messenger RNA controls the translation of cell-cycle and stress proteins in Streptomyces. Barends S, Zehl M, Bialek S, de Waal E, Traag BA, Willemse J, Jensen ON, Vijgenboom E, van Wezel GP. Trade-off between bile resistance and nutritional competence drives Escherichia coli diversification in the mouse gut. De Paepe M, Gaboriau-Routhiau V, Rainteau D, Rakotobe S, Taddei F, Cerf-Bensussan N. A three-protein signaling pathway governing immunity to a bacterial cannibalism toxin. Ellermeier CD, Hobbs EC, Gonzalez-Pastor JE, Losick R. Direct interaction between sensor kinase proteins mediates acute and chronic disease phenotypes in a bacterial pathogen. Goodman AL, Merighi M, Hyodo M, Ventre I, Filloux A, Lory S. Remodelling of the Escherichia coli outer membrane by two small regulatory RNAs. Guillier M, Gottesman S. Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome profiling. Ingolia NT, Ghaemmaghami S, Newman JR, Weissman JS. Regulated virulence controls the ability of a pathogen to compete with the gut microbiota. Kamada N, Kim YG, Sham HP, Vallance BA, Puente JL, Martens EC, Núñez G. The competitive advantage of a dual-transporter system. Levy S, Kafri M, Carmi M, Barkai N The anti-Shine-Dalgarno sequence drives translational pausing and codon choice in bacteria. Li GW, Oh E, Weissman JS. Yersinia pestis genome sequencing identifies patterns of global phylogenetic diversity. Morelli G, Song Y, Mazzoni CJ, Eppinger M, Roumagnac P, Wagner DM, Feldkamp M, Kusecek B, Vogler AJ, Li Y, Cui Y, Thomson NR, Jombart T, Leblois R, Lichtner P, Rahalison L, Petersen JM, Balloux F, Keim P, Wirth T, Ravel J, Yang R, Carniel E, Achtman M. Phenotypic landscape of a bacterial cell. Nichols RJ, Sen S, Choo YJ, Beltrao P, Zietek M, Chaba R, Lee S, Kazmierczak KM, Lee KJ, Wong A, Shales M, Lovett S, Winkler ME, Krogan NJ, Typas A, Gross CA. Secreted transcription factor controls Mycobacterium tuberculosis virulence. Raghavan S, Manzanillo P, Chan K, Dovey C, Cox JS. The ABC transporter MalFGK2 sequesters the MalT transcription factor at the membrane in the absence of cognate substrate Richet E, Davidson AL, Joly N. Local absence of secondary structure permits translation of mRNAs that lack ribosome-binding sites. Scharff LB, Childs L, Walther D, Bock R. Oligonucleotide recombination in Gram-negative bacteria. Swingle B, Markel E, Costantino N, Bubunenko MG, Cartinhour S, Court DL. YycH regulates the activity of the essential YycFG two-component system in Bacillus subtilis. Szurmant H, Nelson K, Kim EJ, Perego M, Hoch JA. Structure and mechanisms of a protein-based organelle in Escherichia coli. Tanaka S, Sawaya MR, Yeates TO. Two variable active site residues modulate response regulator phosphoryl group stability. Thomas SA, Brewster JA, Bourret RB. Sensing wetness: a new role for the bacterial flagellum. Wang Q, Suzuki A, Mariconda S, Porwollik S, Harshey RM. Regulation of yeast pyruvate kinase by ultrasensitive allostery independent of phosphorylation. Xu YF, Zhao X, Glass DS, Absalan F, Perlman DH, Broach JR, Rabinowitz JD. pH sensing by intracellular Salmonella induces effector translocation. Yu XJ, McGourty K, Liu M, Unsworth KE, Holden DW. Conformational control of the Ste5 scaffold protein insulates against MAP kinase misactivation. Zalatan JG, Coyle SM, Rajan S, Sidhu SS, Lim WA. Sequential checkpoints govern substrate selection during cotranslational protein targeting. Zhang X, Rashid R, Wang K, Shan SO. Mg2+ facilitates leader peptide translation to induce riboswitchmediated transcription termination. Zhao G, Kong W, Weatherspoon-Griffin N, Clark-Curtiss J, Shi Y.