Spring 2011 Hematology Lab
Fundamentals of
Hematology readings
Complete Blood Count Report
Leukocyte
expression of quantity
VCS Technology
MICROBIOLOGY 410
HEMATOLOGY LAB
Spring, 2011
Objectives: Understand and interpret common parameters of a Complete Blood Count report. Perform
accurate microscopic examinations of prepared peripheral blood smears (White Blood Cell differential
count and Red Blood Cell morphology evaluation). Distinguish normal from abnormal blood cell
morphology in prepared peripheral blood smears or photographs and drawings. Identify blood cell
inclusions in prepared peripheral blood smears or photographs and drawings. Understand the origin and
consequences of observed blood cell abnormalities.
ACADEMIC HONESTY: Students are expected to read and abide by the University's Academic Honesty
Policy: http://www.calstatela.edu/academic/senate/handbook/ch5a.htm. Students who violate this policy
will be subject to disciplinary action, and may receive a failing grade in the course for a single violation.
Week 1
March 30
CBC/Normal Differential
Carr chapter 1; Evolve Weblinks 1 “Understanding the CBC”;
Evolve Weblinks 23 “Artifacts”; Uthman “1. Blood Cells and the
CBC”; “2. Anemia: Pathophysiologic Consequences,
Classification, and Clinical Investigation”
Week 2
April 6
RBC Maturation/Normal and Abnormal Morphology I
Carr chapter 2-3, 10-14, 24; Evolve Weblinks 1 “Diagnosis From
the Blood Smear”; Evolve Weblinks 10 “Iron Deficiency Anemia”;
Evolve Summary Tables “RBC maturation”, “RBC Variations”;
Uthman “3. Nutritional Anemias and Anemia of Chronic Disease”
Week 3
April 13
RBC Abnormal Morphology II, RBC Inclusions
Carr chapter 10-13; Evolve Summary Tables “RBC Inclusions”,
Uthman “4. Hemolytic Anemias”, “5. Hemoglobinopathies and
Thalassemias”
Week 4
April 20
Review RBC Maturation and Morphology
Week 5
April 27
MID-TERM EXAM (125 points)
Week 6
May 4
WBC Maturation
Carr chapter 4-9; Evolve Summary Table “Myeloid Maturation”
Week 7
May 11
WBC Non-Malignant Disorders
Carr chapter 14-15, 22-23; Evolve Summary Tables “Monocyte v
Reactive Lymphocyte”, “Leukocyte Alterations”
Week 8
May 18
Acute and Chronic Leukemias
Carr chapter 16-21; Evolve Summary Tables “Myeloproliferative
Disorders”, “Acute Myeloid Leukemias”; “Lymphoproliferative
Disorders”, “Acute Lymphoid Leukemias”,
Week 9
May 25
Review WBC Maturation and Morphology
Week 10
June 1
FINAL EXAM (175 points)
Grading:
The total possible points earned are 300 (sum of Mid-Term and Final
examinations). The Final exam will include material covered during the entire
course. Letter grades will not be assigned or reported separately by the
Laboratory Instructor.
Attendance:
Attendance is required.
Instructor:
John Colby, MT(ASCP)
Staff Applications Scientist
Beckman Coulter, Inc.
jwcolby@beckman.com
Office Hours:
By arrangement Wednesday 15:30 – 22:00
Required Text: Clinical Hematology Atlas, Jacqueline H. Carr & Bernadette F. Rodak
Internet:
Evolve Student Resources http://evolve.elsevier.com/Carr/hematology
Home page of pathologist Ed Uthman, M.D.: http://web2.iadfw.net/uthman
(last accessed 3-20-2011) The Red Cell and Anemia is a series of 5 HTML
documents, also available in PDF.
Hematology Web Site of the New York Department of Health, Wadsworth
Center, Through the Microscope: Blood Cells:
http://www.wadsworth.org/chemheme/heme/microscope/celllist.htm (last
accessed 3-20-2011)
Home page of the American Society of Hematology: http://www.hematology.org
(last accessed 3-20-2011) Educational materials include an Image Bank and
Teaching Cases are located under PUBLICATIONS.
Home page of Blood Line: http://www.bloodline.net/ (last accessed 3-20-2011)
Image Atlas and Case Studies sections.
Centrifuged Blood
Coulter Principal
Slide preparation
Microscope Slide Prep
Cell Architecture
Areas to Examine
Too Thick
Film too thick
Good Area
Good area for exam
Too Thin
Film too thin
RBC, Platelet, WBC
Normal WBC
Blood Cells
PMN or Neutrophils
Neutrophils
PMN
Neutrophils
PMN with drumstick
Lymphocytes
Lymphocytes
Lymphocyte & Neutrophil
Monocyte
Monocyte
Monocytes may be increased in chronic infections, chronic inflammatory disorders and some
forms of MDS and myeloid leukemia
Copyright ©2005 American Society of Hematology. Copyright restrictions may apply.
Monocyte with a typical folded, lobulated nucleus and a ground glass appearance to the
cytoplasm
Copyright ©2005 American Society of Hematology. Copyright restrictions may apply.
Monocyte
Eosinophil
Eosinophil
Eosinophil
Eosinophil and Lymphocyte
Basophil
Basophil
Platelets
Giant Platelet
Basophil, Neutrophil, & Lymphocyte
Broken Cell
Basket Cell
Crushed Eosiniphil
Crenated RBC
Water Artifact
Stain Artifact