BIO 185
THE BIOLOGY OF THE CELL
Winter 2014
Instructors:
Office:
Phone - office:
- home:
Walter J. Bruyninckx
Science Center,217
866-7242
866-4522 (before 9:00 PM)
e-mail:
bruyninx@hanover.edu
Office Hours:
Monday
Wednesday
Thursday
Friday
9:00 -10:00 am
12:00 - 4:00 pm
12:00 - 3:00 pm
9:00 -10:00 am
Bio 185
Bio 185. Cell and Molecular Biology
Molecules of the living material
Cellular organization of living material
Energy metabolism
Molecular biology: central dogma
Cancer biology
Bio 185
Cell and Molecular Biology
ACTIVITIES:
1.Lectures
2.Preparing a Notebook
3.Study
4.Readings
5.Lab
6.Exams.
Scientific
America
Due
Authors
Title
1/11
Gerstein Mark and
Levitt Michael
1/18
Welch William J.
May 1993
How cells respond to stress
56-64
2/01
Goldberg Alfred L., Elledge
Stephen J. and Harper J.
Wade
January
2001
The cellular chamber of doom
68-73
2/15
Rothman James E. and Orci
Lelio
March
1996
Budding vesicles in living cells
70-75
BioScienc
e 35 p730
November
Simulating water and molecules of life
1998
Pages
100-105
2/22
McCarthy Richard E
1985
H+ ATPases in oxidative and
photosynthetic phosphorylation
3/15
Lau Nelson C. and Bartel
David P.
August
2003
Censors of the genome
34-41
3/22
Ast Gil
April 2005
The alternative genome
58-65
4/05
Scott John D. and Pawson
Tony
June 2000
Cell communication: the inside story
72-79
4/12
Weinberg Robert A.
September
1996
How cancer arises
62-70
Bio 185. Molecular Cell Biology
Grading
Exam
date
Exam 1
Exam 2
Exam 3
Exam 4
Exam 5
Cmprh. ex
Lab exam
Lab reports
Readings
01/25
02/08
03/08
03/28
04/15
04/15
04/09
Total
Lectures
The molecular basis of Life (Lecture1-11)
Cell structure (Lecture 12-20)
Energy metabolism (Lecture 21-28)
Molecular Biology (Lecture 29-40)
Cancer (Lecture 40-49)
Lectures (1-49)
points
%
80
80
80
80
80
50
50
50
50
13.33
13.33
13.33
13.33
13.33
8.33
8.33
8.33
8.33
600
Bio 185
Cell and Molecular Biology
http://vault.hanover.edu/~bruyninx/
Bio185Bruyninckx
Bio185Content
Bio185Exercises
Bio185LabData
Bio185PowerPoint
Bio185ReadingAssignments
Bio185Syllabus
Bio 185. Molecular Cell Biology
Course policies
3. Course policies.
0. Do NOT wear a baseball cap (in whatever direction) in class or lab.
1. Your active and attentive presence in class and lab is expected.
2. Any work handed in after the time is due will not be graded.
3. All written material turned in for credit must be typewritten and papercopy (I am sorry, we cannot be your printer). Multiple pages need to be
stapled together!
4. All writing that is not your own independent work must be properly
referenced. Please refer to the Student Handbook (pages 2 to 3) and the
policy statement on academic dishonesty.
4. There is no opportunity to make up quizzes, laboratories, assignments.
5. For safety reasons, eating, drinking and smoking are not permitted in
the laboratory or class. Shoes must be worn at all times in the laboratory,
as there may be broken glass and other hazardous material on the floor.
6. Turn off your cell phone or other similar devices when in class, lecture,
or study session.
7. Limit use of a computer in class or lab to the scheduled class activities.
8. Please, no smelly food!
Wk Date
Title
Topic
1
2
3
4
5
6
7
01/08
01/15
01/22
01/29
02/05
02/12
02/19
Introduction
Laboratory tools
Introduction to bio-molecules
Characterization of enzymes-1
Characterization of enzymes-2
Cell membrane and osmosis
Cell Structure
Topic-1
Topic-1
Topic-2
Topic-3
Topic-3
Topic 4
Topic 4
8
9
10
11
12
13
03/05
03/12
03/19
03/26
04/02
04/09
Respiration and fermentation
Photosynthesis
Analysis of proteins
DNA and Cell cycle
Gene regulation: lac operon
Lab Exam
Topic 5
Topic 6
Topic 9
Topic 7, 8
Topic 10
LAB REPORT FORMAT: (1 report per group!)
LAB TITLE: Properties and Function of Enzymes.
Experiment 1.
Goal: one sentence.
Method: Relevant.
Results: (based on class data). A (mean
and SEM) is significantly different
(P<0.05) from B (mean and SEM).
Conclusion: A is different from B.
Explanation.
Standard Curve Yellow Dye
0.9
0.8
Optical Density at 420 nm
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
1
1.5
2
2.5
3
Concentration (drops)
3.5
4
Figure 1. Standard curve of yellow dye at 420 nm. We measured
the optical density at 420 nm of four concentrations of yellow dye
(1, 2, 3, and 4 drops per sample) using a Spectronix 21
spectrophotometer. The OD of Standard 1 ((0.217 ± 0.0070) was
significantly smaller (P<0.05, Student’s t-test) than that of Standard
2 (0.410 ± 0.0057), the OD of Standard 2 was significantly smaller
(P<0.05) than that of Standard 3 (0.619 ± 0.0045) and the OD of
Standard 3 was significantly smaller (P<0.05) than that of Standard
4 (0.806 ± 0.0087). We calculated the standard curve (linear
regression y = 0.1977x + 0.0186, R2 = 0.9996).We measured the
optical density at 420 nm of an unknown concentration of yellow
dye (OD equals 0.699 ± 0.0054) and used the standard curve to
determine its concentration (3.44 drops per sample).
Venus in
front of
the
mirror
(1613)
Bacchus
(1638)
The Union
of Earth
and Water
(1618)