Recombinant DNA Technology
Bacteria, Yeast, & Virus Plasmids
What is a Plasmid?
• Circular DNA found in bacteria, viruses, and
yeast
Recombinant Plasmids
• Plasmids that have
foreign DNA inserted
into them in a process
called Recombinant
DNA Technology
Methods of Recombinant Technology
• Transformation
– Plasmid and foreign DNA
are cleaved by restriction
enzymes
– Uses DNA ligase to insert
DNA into vector
– Vector inserted into host
cell (usually E. coli)
Methods of Recombinant Technology
• Non-Bacterial
Transformation
– Similar to transformation
except E. coli/bacteria is
not used as a host cell
– DNA is injected directly
into the nucleus of the cell
being transformed
OR
– The host cells are
bombarded with high
velocity microprojectiles
such as gold or tungsten
coated with DNA
Methods of Recombinant Technology
• Phage Introduction
– Process of transfection
(equivalent to
transformation) except a
phage is used instead of
bacteria
– Lambda or M13 phages
are used to produce
phage plaques which
contain recombinants
(From: AN INTRODUCTION TO GENETIC ANALYSIS 6/E BY Griffiths, Miller, Suzuki,
Leontin, Gelbart ã 1996 by W. H. Freeman and Company. Used with permission.)
4. Cosmids are hybrids of phages and plasmids that can carry DNA fragments up
Antibiotic Resistance
The Evolutionary Arms Race
http://www.youtube.com/watch?v=9CuhqQzBACQ (Ep1)
http://www.youtube.com/watch?v=2L82V6VPJkQ (Ep2)
http://www.youtube.com/watch?v=D0_FTJnhzXA
(Ep3)
http://www.youtube.com/watch?v=ofL52tWhacY
(Ep4)
http://www.youtube.com/watch?v=Chmb2g7atZ4
(Ep5)
http://www.youtube.com/watch?v=J-VOvideRZA
(Ep6)