AP Biology Discussion Notes
Friday 3/6/2015
Goals for Today
• Be able to describe the difference in the 2
+pGLO plates using our Operon Vocabulary
• Be able to describe what it means and why
genes from a jellyfish can function in
bacteria.
• Be able to analyze transformation efficiency
and describe what that calculation actually
means.
Question of the day 3/06
• What kind of Operon is normally in the
“on” position, how is this accomplished,
and what type of control would this
exhibit?
• Give an example of this type of operon.
• Is this the operon in our lab
Desired Lab Results
Lab Analysis
• Be able to explain the predictions/results
discussing OPERONS!
What is this thing we inserted?
Arabinose Operon
Arabinose Operon
GFPgene
Arabinose Operon
GFPgene
• OPERON VOCABULARY to use in your
lab explanation. Describe what is going
on in the 2 +pGLO plates using the terms:
– Inducible/repressible
– Positive/negative control
– Promoter
– Operator
– Repressor Protein
– Transcription
Lab Analysis
• Be able to explain the predictions/results
discussing OPERONS!
1. Work through lab analysis & calculations
together. (Yellow sheet) and ASK
QUESTIONS!
2. Next answer white sheet as a group.
What is transformation efficiency?
• What is the unit?
• Is this number bigger or smaller than your
number of colonies?
• Did we add a whole µg?
Questions on the Lab?
LAB QUIZ on TUESDAY