University of Puerto Rico
Río Piedras Campus
College of Natural Sciences
Nutrition and Dietetics Program
Program: Bachelor of Science in
Nutrition and Dietetics
Human Biochemistry Laboratory, NUTR
4159
First Semester, 2013-2014 Academic Year
Sec. 0U1, Wednesday, 1:00 p.m. – 3:50 p.m.
Room NEP 577
Michelle Schelske Santos, Ph.D.; mschelskeuprrp@gmail.com, mschelske@uprrp.edu; ext. 5798, 2792
Date
14Aug13
21Aug13
28Aug13
04Sep13
11Sep13
18Sep13
25Sep13
02Oct13
Fri., 04Oct13
(rep. 27Nov13)
09Oct13
Assignment
Topic
Syllabus, Preparation of LAB Notebooks;
Register for course in Edu 2.0
General Laboratory Safety; Intro to
instrumentation; Equipment assignment
(bring lab coats and lab notebooks)
Monday schedule (rep. 02sep13); NUTR 4158
Introduction to course
Introduction to Prep of Lab Notebook
Lab Safety
Scientific Instrumentation
LAB Safety Seminar (Biohazard)
Post-conference written quiz; practical lab quiz
Micropipette and Pipette Techniques
(Wed) LAB Computer Workshop – Info Databases
and your Search Strategy (Biblioteca Ciencias
Naturales; Prof. Pura Centeno) Assign: Search
Strategies, DNA & Protein Methods
LAB Excel workshop – Data Tables and Graphs
Micropipette and Pipette Techniques
Quiz in Edu2.0 (Safety & Scientific Instrumentation)
open 24h before lab
Due: Assig Search Strategies DNA/Ptn Method
LAB Expt: Active Acidity and pH;
Pre-lab Quiz in Edu2.0 open 24h before lab
LAB – Enzyme-linked ImmunoSorbent Assay
(ELISA)
LAB – Tissue Homogenation of Protein Samples
Quiz in Edu 2.0; open 24h before lab
LAB – Total Protein Determination
Quiz in Edu 2.0; open 24h before lab
LAB Expt. – Membrane Structure and Function;
Quiz in Blackboard; open 24h before lab;
Blood-borne Pathogens and Biohazard
Disposal (OPASO; Dr. Warner Ithier)
Micropipette and Pipette Techniques
Biochemical methods for DNA and protein
analyses in the field of nutrition
--- (see NUTR 4158 calendar)
Introduction to Excel for Lab Reports--Water
content of foods
Micropipette and Pipetting Techniques
Active Acidity and pH
Colorimetric Determination of Presence of
Specific Antigen using Antibodies & Enzymes
Homogenation of animal & vegetable protein
Protein content of animal versus vegetable
protein on microscale using microplate reader
Blood Typing
Digital Microscopy of Blood Cells
Dra. M. Schelske Santos; NUTR 4159
Fri, 11Oct13
06Nov13
LAB OP1 – (50 pts.; 1h)
Lab Safety – Total Protein Determination
TBA
LAB - DNA Extraction
DNA Extraction from whole human blood
(need confirmation of nurse collaboration from Preparation of blood fractions for protein
Medical Services)
analysis via SDS-PAGE
LAB: Electrophoresis of DNA
Horizontal agarose gel electrophoresis of
nucleic acids
LAB: Protein Electrophoresis of blood fractions
SDS-PAGE or protein electrophoresis, gel
staining & drying
Measurements of migration distances in gels
SDS-PAGE and DNA gel interpretation
Quiz in Edu 2.0; open 24h before lab
3-D structure of proteins, genetic diseases
TBA (To Be Announced)
13Nov13
(Monday) Oral Presentations – NUTR 4158
20Nov13
Virtual Lab –
Colorimetric Determination of Vitamin C
16Oct13
23Oct13
30Oct13
27Nov13
04Dec13
TBA, Dec. 11
-18, 2013
Vitamin C Content in Raw vs. Processed
Foods; Comparisons to Food Labels and
Nutrient Data Base
Bioinformatics Assignment
Academic Recess – Happy Thanksgiving!
LAB – Lipids and Fiber
Quiz in Edu 2.0; open 24h before lab
Dietary Lipids, Fiber & Fattaché
Lab OP 2 – (50 pts.; 1h)
Due: Lab Notebooks
XXXXXXX
GMO Investigator
XXXXXXX
XXXXXXX
LAB –Glycolytic fermentation
Due SDS-PAGE Lab (in notebook)
Due Bioinformatics (Edu 2.0)
LAB – Size Exclusion Column Chromatography
XXXXXXX
LAB – DNA Extraction
Identification of Genetically Modified Foods
using PCR (need collaboration for PCR)
Anaerobic fermentation of sugars by yeast;
mono- and di-saccharides, aspartame,
sucralose and citric acid inhibition of enzyme
Separation of vitamin B12 and hemoglobin by
size as they pass through a chromatography
column
DNA Extraction from strawberries
This schedule is subject to change based on unforeseen events, natural disasters, scheduling of student
assemblies, and changes in laboratory equipment and material arrival dates. In the event of any change, a new
schedule will be developed and available in Edu 2.0.
edited Sept. 27, 2013
sem. 1, 2013-2014
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