DO NOT REMOVE
INSTRUMENT:
Veeco CP-II Scanning Probe Microscope
INSTRUMENT START-UP:
A.
Remove the blue metal cover from the SPM
B.
Make sure the switches on the laser head are as follows:
AFM → C-AFM → LFM → C-AFM/LFM
C.
Turn on the laser
1.
The switch is located on the upper right corner of the probe head
D.
Log on to the computer
1.
User Name: Administrator
2.
Password: c106b
E.
To open the software
1.
Select Start → Data Acquisition
2.
Select Start → WinTV2000
1

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RUNNING THE CALIBRATION GRADIENT:
A.
Raise the probe head to 18000 µm by using the Z bar and clicking on the upper half of the rectangle
B.
Using tweezers take the calibration gradient disk and hold it level to the sample holder.
Then SLIDE the disk onto the sample holder
1.
DO NOT LET THE GRADIENT DISK SNAP DOWN ONTO THE SAMPLE HOLDER.
2.
There will be a magnetic pull, be careful to slide the disk on level with the sample holder. The scanner underneath the sample holder is made of a very fragile material that can break under the shock of the “slapping” disk
Calibration
Gradient
C.
The disk needs to sit on the sample holder so the sample is centered on the sample holder
2

D.
Align the laser
1.
Move the optics arm to the probe head
DO NOT REMOVE
Resolution Adjustment Knob
2.
On the Data Acquisition page, select ON for optics
3.
Using the knob on the end of the optics arm, adjust the resolution of the light for the microscope a.
Turning the knob towards you, the image resolution becomes greater for objects closer to the surface b.
Turing the knob away from you, decreased the image resolution
4.
Make sure the laser is hitting the back of the cantilever, if not go to the probe head and adjust the alignment knobs for the cantilever alignment located on the right side a.
The top knob moves the laser up and down b.
The bottom knob moves the laser left and right c.
DO NOT TOUCH located on the black base
3

DO NOT REMOVE
Cantilever (Laser) Alignment:
Up and Down
Detector Alignment:
Up and Down
Cantilever (Laser) Alignment:
Left and Right
Detector Alignment:
Left and Right
DO NOT TOUCH
5.
The laser should be positioned on the end of the probe. After aligning the lasers (No red lights), this is what you should see on the WinTV2000 screen.
6.
There will be a green light on the probe head surrounded by 4 dots that may or may not be red. If there are red lights, that must be corrected before approaching and imaging the sample. a.
Clear the light completely on one side (Up or Down, Left or Right), then do the opposite
4

DO NOT REMOVE
EX: First move the up/down knob to clear the light on the bottom. Then use the left/right knob to clear the light on the left. Continue this method until the light is only green b.
IF THERE ARE RED LIGHTS WITH THE GREEN LIGHT, WHEN YOU APPROACH THE
SAMPLE YOU WILL BREAK THE TIP!!!
E.
Take an image
1.
From the main tool bar, select Tools → Digital Voltmeter (DVM)
2.
Press CH a.
A+B should be 1.6 volts b.
A-B should be oscillating around 0
3.
Approach the probe a.
Click the Approach button b.
The Z (µm) box located underneath the z-bar should be around 13200. c.
Be careful not to go too close or you could break the probe!
4.
Select Mode → Image
5.
When the probe is in the appropriate place to take an image set the size and rate a.
Size: 4.75 b.
Rate: 3 c.
Hit enter after typing in the parameters or the computer will not register the changes
6.
From the main tool bar, select Setup → Input Configuration. a.
Select Topography b.
Both Arrow boxes should be selected c.
Uncheck AC Track
5

DO NOT REMOVE
7.
The red and blue line on the graph should be flat and horizontal on the screen. If they are not flat and horizontal adjust the slope and A
8.
Press Image and let the instrument run to develop the image
F.
Image analysis
1.
Click on the developed image
2.
Select Tools → Image Processing
3.
You can view your picture as a 2-D image, 3-D image, and line analysis
4.
Press Print Screen and save your image as a picture
6

DO NOT REMOVE
G.
Re-image the sample using the following parameters:
1.
Size: 1
2.
Rate: 3
3.
Hit enter after typing the parameters or the computer will not register the changes
H.
Removing the calibration gradient
1.
Raise the probe head to 18000 using the Z-bar
2.
Move the optics arm away from the probe head
3.
Using tweezers, remove the calibration gradient from the sample holder
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RUNNING A CD SAMPLE:
A.
Raise the probe head to 18000 µm by using the Z bar and clicking on the upper half of the rectangle
B.
Using tweezers take the CD disk and hold it level to the sample holder. Then SLIDE the disk onto the sample holder
CD Sample
7

DO NOT REMOVE
1.
DO NOT LET THE CD DISK SNAP DOWN ONTO THE SAMPLE HOLDER.
2.
There will be a magnetic pull, be careful to slide the disk on level with the sample holder. The scanner underneath the sample holder is made of a very fragile material that can break under the shock of the “slapping” disk
C.
The disk needs to sit on the sample holder so the sample is centered on the sample holder
D.
Align the laser
1.
Move the optics arm to the probe head
Resolution Adjustment Knob
2.
On the Data Acquisition page, select ON for optics
3.
Using the knob on the end of the optics arm, adjust the resolution of the light for the microscope a.
Turning the knob towards you, the image resolution becomes greater for objects closer to the surface b.
Turing the knob away from you, decreased the image resolution
4.
Make sure the laser is hitting the back of the cantilever, if not go to the probe head and adjust the alignment knobs for the cantilever alignment located on the right side a.
The top knob moves the laser up and down b.
The bottom knob moves the laser left and right
5.
The laser should be positioned on the end of the probe. After aligning the lasers (No red lights), this is what you should see on the WinTV2000 screen.
8

DO NOT REMOVE
6.
There will be a green light on the probe head surrounded by 4 dots that may or may not be red. If there are red lights, that must be corrected before approaching and imaging the sample. a.
Clear the light completely on one side (Up or Down, Left or Right), then do the opposite
EX: First move the up/down knob to clear the light on the bottom. Then use the left/right knob to clear the light on the left. Continue this method until the light is only green b.
IF THERE ARE RED LIGHTS WITH THE GREEN LIGHT, WHEN YOU APPROACH
THE SAMPLE YOU WILL BREAK THE TIP!!!
E.
Take an image
1.
From the main tool bar, select Tools → Digital Voltmeter (DVM)
2.
Press CH a.
A+B should be 1.6 volts
9

DO NOT REMOVE b.
A-B should be oscillating around 0
3.
Approach the probe a.
Click the Approach button b.
The Z (µm) box located underneath the z-bar should be around 13200. c.
Be careful not to go too close or you could break the probe!
4.
Select Mode → Image
5.
When the probe is in the appropriate place to take an image set the size and rate a.
Size: 4.75 b.
Rate: 3 c.
Hit enter after typing in the parameters or the computer will not register the changes
6.
From the main tool bar, select Setup → Image Configuration. a.
Select Topography b.
Both Arrow boxes should be selected c.
Uncheck AC Track
7.
The red and blue line on the graph should be flat and horizontal on the screen. If they are not flat and horizontal adjust the slope and A
10

DO NOT REMOVE
8.
Select Image and let the instrument run to develop the image
F.
Image analysis
1.
Click on the developed image
2.
Select Tools → Image Processing
3.
You can view your picture as a 2-D image, 3-D image, and line analysis
4.
Press Print Screen and save your image as a picture
G.
Re-image the sample using the following parameters:
4.
Size: 1
5.
Rate: 3
6.
Hit enter after typing the parameters or the computer will not register the changes
H.
Removing the calibration gradient
4.
Raise the probe head to 18000 using the Z-bar
5.
Move the optics arm away from the probe head
6.
Using tweezers, remove the calibration gradient from the sample holder
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11

INSTRUMENT SHUT-DOWN:
A.
Close the software
B.
Turn off the laser
C.
Log off the computer
D.
Replace to blue cover on the SPM
DO NOT REMOVE
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