Topic: Microscope
Aim: How do we use the compound microscope?
Do Now:
1. How to use a microscope reading ISA
(4 minutes)
2. Take out yesterday’s microscope notes
HW: 1. Finish the Microscope ISA
2. Castle Learning: Cell Respiration and
Transport  Due Friday
Total magnification:
Eyepiece x Objective
100x
400x
Power = 4x
x
10x = 40x
4x
10x
40x
10x
10x
100x
40x 10x
400x
1. Describe what occurred to the size of the image
when switching from 4x to 10x to 40x objectives.
The specimen APPEARS larger.
Low Power
High Power
4x
10x
40x
10x
10x
100x
40x 10x
400x
2. Does the field of view increase or decrease as you
switch from the low power objective to the high power
objective? Support your answer.
Field of view decreases. You see less of the slide.
4x
10x
40x
10x
10x
100x
40x 10x
400x
3. Which objective should be used when
searching for the specimen on the slide? Support
your answer.
Low power because it has a larger field of view.
How do images appear?
Image on slide
BEFORE placing it
on the stage to view
e
How the image
appears when
viewing with the
microscope
On the slide
F
Seen through lens
What happens when you
move the slide?
• Image moves in opposite direction
How must you always carry a
microscope?
• Always carry with 2 hands. One hand on
arm and one hand under the base
Low power objective
High power objective
e
e
1. If you switch to high power when the image is positioned in
the diagram above, how will the image appear under high
power? It will not be in your field of view.
2. Identify a procedure that must be followed before switching to
high power. Center your image.
Low power objective
High power objective
Low power objective
High power objective
CENTER YOUR IMAGE
before switching to high
power!!!!
What two procedures must be done
BEFORE switching to high power?
- Focus your image
- Center your image
What happens to the amount of
light in your field of view when
switching to high power?
• The amount of light DECREASES.
• Adjust the diaphragm to make it
brighter.
What do you use to clean the
microscope lenses?
• Only use LENS PAPER for cleaning
Let’s summarize…Identify the microscope part
described.
Fine adjustment
1. Used to focus the specimen under high power.
2. Adjusts the amount of light reaching the specimen.
Diaphragm
3. Platform slide is placed on. Stage
4. Objective used to view a larger field of view needed
to search for a specimen. Low power objective
5. Used to focus specimen under low power Coarse
adjustment
6. Structure that you look through. Ocular
7. Contains lenses. Ocular and objectives
8. Keeps slide in place. Stage clips
9. Objective used to view a larger and more magnified
view of the specimen. High power objective
10. Objective used to search for a specimen.Scanning obj.
C6H12O6 + 6O2
 6CO2 + 6 H2O + X
Aerobic respiration
1. Identify the process represented above.
2. Identify the raw materials. glucose and oxygen
3. Where does this process occur? Mitochondria
4. Identify the waste products.carbon dioxide and
water
5. Identify molecule X. Energy or ATP
6. Describe the importance of this process.
It provides energy that is needed for all cell activities.
Why do we stain cells?
Unstained
cheek cells
Stained
cheek cells
Cheek Cells
Unstained
Stained with Methylene Blue
Skin Cells stained with Methylene Blue
Onion Cells
Unstained
Stained with Lugol’s
Iodine
Why do we stain cells?
• To Make cell parts more visible
Wet
mount
1. Place a drop of water on the slide
2. Place object on the slide.
3. Slowly lower the cover slip on an
angle to prevent AIR BUBBLES.
4. Absorb excess water with a
paper towel.
Let’s summarize…
1. Explain one rule that must be followed when
using the microscope.
2. What do we use to make cell parts more
visible?
3. Which way will a cell appear to move when
moving the microscope to the left and down?
4. What happens to the image of the specimen
when viewed with a microscope?