BioTech Lab Methods and Techniques
COURSE SYLLABUS, FALL 2014
Tulsa Community College, SEC Campus
BIOT 1315-301-12089
Lecture:
Lab:
Science and Mathematics Division
Chair:
Lyn Kent
Office:
SE 8125
Phone No.:
595-7742
Biotechnology Coordinator:
Office:
OFFICE HOURS
Mon:
1:00- 3:30
Tue:
9:30- 10:30
Wed:
1:00- 3:30
MW
MW
Instructor:
Office:
Phone No.:
e-mail:
8:30- 9:20
9:30-12:20
SE 8260
SE 8260
William T. Briscoe, Ph.D.
SE 8265
595-7680
bill.briscoe@tulsacc.edu
Diana Spencer, Ph.D
SE 8275
Phone No.:
595-8605
Thu:
1:00- 3:30
Fri.
9:00-12:00
or by appointment
COURSE PREREQUISITE:
Chemistry I (CHEM 1315), Biology for Majors (BIOL 1224) and Intro to Biotech (BIOT 1113).
COURSE DESCRIPTION
Emphasis is on laboratory operations, management, equipment, instrumentation, quality control
techniques, and safety procedures. Lecture emphasis will be on biochemistry, bioorganic topics and the
scientific method, including assay design. Laboratory practice will stress lab safety and the design of
assays for various classes of biochemicals, including RNA, DNA, enzyme and total protein assays.
Lecture 2 hr, Lab 6 hr.
NEXT COUSE IN SEQUENCE
Cell Culture Techniques (BIOT 1534)(or concurrent).
TEXTBOOK AND OTHER MATERIALS
 Modern Experimental Biochemistry, 3rd edition, 2000, Rodney Boyer, Benjamin/Cummings, San
Francisco.
 Lodish, H., et al, 4th ed., W.H. Freeman & Co., New York—available FREE on-line. See web
addresses listed at end of syllabus.
 Stapler with which to staple homework papers and lab reports together
 Safety Goggles and Lab Apron: are to be worn in the laboratory at all times!!!!!
They are available in the TJC bookstore.
 flash drive
Optional Materials:
Basic Laboratory Methods for Biotechnology—Textbook and Laboratory
Reference, 2nd ed., 2009, Lisa A. Seidman & Cynthia J. Moore, Prentice Hall,
Upper Saddle River, N.J.
Cliffs Quick Review, Biochemistry I, 2000, Frank Schmidt, IDG Books Worldwide,
Inc., N.Y.
Cliffs Quick Review, Biochemistry II, 2000, Frank Schmidt, IDG Books
Worldwide, Inc., N.Y.
Introduction to Biotechnology, 2004, William J. Thieman & Michael A. Palladino,
Pearson/Benjamin Cummings, San Francisco.
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COURSE OBJECTIVES
Biotechnology is divided between theoretical and practical skills. This course will teach many the skills
and procedures employed in biotechnology as well as the fundamental theories and mechanisms that are
the basis for the procedures. The terminology and vocabulary used by the biotechnologist will be
developed and applied.
The technique for solving quantitative problems relating to biotechnology will be presented. Since the
problems in biotechnology are many and varied, one objective of this course is to develop the ability to
study independently. At the same time, the student will need how to work around and with others a
become an effective member of a team.
During the laboratory sessions the student will have the opportunity to perform simple laboratory
procedures and practice safe handling of laboratory biochemicals and equipment. During the first
laboratory session a list of safety rules will be presented and discussed. Strict precautions and guidelines
must be followed in the lab.
PROFESSIONALISM:
It is assumed that students enrolling in a course at this level are headed toward a professional career.
Because prospective future employers, clients, patients, and admissions committees will make decisions
of your future association with them based on, among other things, your reputation of projecting a
professional image, it is important to be projecting that image now. A professional image includes such
character traits as attendance, punctuality, thoroughness, attentiveness, attitude toward authority,
honesty, and appearance (dress and grooming).
ATTENDANCE POLICY
Attendance is mandatory. Attendance records will be maintained. It is the responsibility of the student to
inform the instructor if he/she arrives late. The student has the sole responsibility to initiate and carry out
the withdrawal procedure. If the withdrawal procedure is not completed, a failing grade may be assigned
at the end of the semester for non-attendance of class.
COURSE WITHDRAWAL: The deadline to withdraw from a course shall not exceed 3/4 the duration of
any class. Check the TCC Academic Calendar for the deadline that applies to the course(s). Begin
the process with a discussion with the faculty member assigned to the course. Contact the
Advisement Office at any TCC campus to initiate withdrawal from a course ('W' grade) or to change
from Credit to Audit. Withdrawal and/or change to an audit from a course after the drop/add period
can alter the financial aid award for the current and future semesters. Students may receive an
outstanding bill from TCC if the recalculation leaves a balance due to TCC. Students who stop
participating in the course and fail to withdraw may receive a course grade of “F,” which may have
financial aid consequences for the student.
Communications:
a. Email communications: All TCC students receive a designated “MyTCC” email address (ex:
jane.doe@mail.tulsacc.edu). All communications to you about TCC and course assignments
will be sent to your MyTCC email address; and you must use MyTCC email to send email to,
and receive email from, the instructor regarding this course.
b.
Inclement Weather: TCC rarely closes. If extreme weather conditions or emergency situations
arise, TCC always gives cancellation notices to radio and television stations. This information is
also posted on the TCC website (www.tulsacc.edu). Notification is also available via phone and
text messaging for those who have registered with TCC Alerts.
General Education Goals: General Education courses at TCC ensure that our graduates gain skills,
knowledge, and abilities that comprise a common foundation for their higher education and a backdrop for
their work and personal lives. TCC’s General Education goals are: Critical Thinking, Effective
Communication, Engaged Learning, and Technological Proficiency.
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Classroom Etiquette: Open and mutually respectful communication of varied opinions, beliefs, and
perspectives during classroom or online discussion encourages the free exchange of ideas that is
essential to higher learning and to the ability to learn from each other. Use of any electronic device is at
the discretion of the instructor.
Syllabus Changes: Occasionally, changes to the syllabus may be necessary. Students will be notified
of any changes to the syllabus in class.
Disability Resources: It is the policy and practice of Tulsa Community College to create inclusive
learning environments. Accommodations for qualifying students in compliance with the Americans with
Disabilities Act (ADA) and Section 504 of the Rehabilitation Act are available. To request
accommodations, contact the Education Access Center (EAC) at eac@tulsacc.edu or call (918) 595-7115
(Voice). Deaf and hard of hearing students may text (918) 809-1864.
Academic Dishonesty: Academic dishonesty (cheating) is defined as the deception of others about
one’s own work or about the work of another. Academic dishonesty or misconduct is not condoned or
tolerated at campuses within the Tulsa Community College system. Tulsa Community College adopts a
policy delegating certain forms of authority for disciplinary action to the faculty. Such disciplinary actions
delegated to the faculty include, but are not limited to, the dismissal of disrespectful or disorderly students
from classes. In the case of academic dishonesty a faculty member may:
 require the student to redo an assignment or test, or require the student to complete a substitute
assignment or test;

Record a "zero" for the assignment or test in question;

Recommend to the student that the student withdraw from the class, or administratively withdraw
the student from the class;

Record a grade of "F" for the student at the end of the semester. Faculty may request that
disciplinary action be taken against a student at the administrative level by submitting such
request to the Dean of Student Services.
Institutional Statement: Each student is responsible for being aware of the information contained in the
TCC Catalog, the TCC Student Policies & Resources Handbook, and semester information listed in the
class schedule. All information may be viewed on the TCC website: www.tulsacc.edu
LEARNING SUPPORT SERVICES AND COMPUTER ASSISTED INSTRUCTION
A variety of CD-Rom tutorials are available for purchase in the bookstore to aid in studying. The tutorials
are extremely helpful in preparing for class and exams. Access to computing resources is a privilege
granted to all TCC faculty, staff, and students. Use of TCC computing resources is limited to purposes
related to the College’s mission of education, research, and community service. Student use of
technology is governed by the Computer Services Acceptable Use Statements/Standards found in the
TCC Student Code of Conduct Policy Handbook. These handbooks may be obtained by contacting any
Student Activities or Dean of Student Services office.
TEACHING METHODS
All lecture and laboratory material must be read prior to class presentation or laboratory period.
Problems from the textbook should be worked. Specific problems may be assigned to be handed
in for grading.
EVALUATION TECHNIQUES
A. Exams—Three (3) 140 points each
These examinations will cover the lecture material and all the material in the assigned chapters in
the textbook. There will be questions that cover the background information on the laboratory
methods and techniques involving understanding of the techniques employed and the calculations
3
used in these techniques.
The exact formats will be explained in class. No make-up tests will be given.
B. Final Exam (150 pts.) is comprehensive, mandatory and CANNOT be made up.
C. Laboratory Notebook Spotchecks—(random, graded three times during semester, 25 pts. ea.)
1. Laboratory notebooks will be checked three times during the semester, unannounced, to ensure
that you have been keeping your entries into the notebook up to date. The laboratory notebook is
to be more of a lab journal of your work, not an exquisite work of art to decorate your coffee table.
D. Laboratory Notebook—(graded three times during semester, 100 pts. ea)
1. Bring your Laboratory Notebook with you to all class meetings. Laboratory work will be
completed during the assigned laboratory period. It is mandatory that all laboratory notes and
data be recorded in ink, in a notebook, not on scraps of paper. The lab procedure should be
studied prior to coming to lab. The write-up and conclusions for each laboratory assignment
should be finished for grading within 7 calendar days of completion of a lab. Included in the
grade will be an evaluation of laboratory practices, safety habits, overall effort and lab write-up.
The laboratory notebooks will be collected for grading several times during the semester. Keep it
up to date!
2. Late lab notebooks will NOT be accepted for full credit.
3. Laboratory experiments cannot be made up. An absence during one of the lab periods will result
in the forfeit of all points associated with that laboratory exercise.
4. Students are advised to exercise extreme caution in the laboratory and to follow all guidelines
listed on the "Safe Lab Practices" handout.
5. Students MUST purchase and wear goggles and a lab apron during labs. Students NOT
wearing the above will NOT be allowed to participate in the laboratory and will forfeit all
points associated with that laboratory exercise.
E. Homework
1. Specific homework problems from the text may be assigned throughout the course. These
problems will aid the student in obtaining a thorough understanding of the material.
F. Late Work
1. Late homework and labs will be accepted up to one week after the scheduled turn-in date at a
10% reduction in grade, up to two weeks with a 25% reduction in grade. Late homework due to
excused absence on the date due may be turned in on the next class day attended without
penalty. The instructor may waive penalties for valid extenuating circumstances at his sole
discretion. No work will be accepted after the last day of regular classes.
GRADING SYSTEM
Exam Grades (3 @ 140 pts. ea.)
Laboratory Notebook spotchecks (3@25 ptx ea.)
Laboratory Notebook (3@100 pts.ea)
Professionalism (55 pts.)
Final exam (150 pts.—mandatory)
GRADING SCALE
90 -100%
80 - 89%
70 - 79%
60 - 69%
Below 60%
=
=
=
=
=
420 points
75 points
300 points
55 points
150 points
1000 points
A
B
C
D
F
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References to on-line TEXTBOOK
Lodish, Mol. Cell Biology
Chaps 1 & 2
1. Chap 1.0 The Dynamic Cell
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?highlight=dynamic,cell&rid=mcb.chapter.145
2. Chap 1.3 The Architecture of Cells
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?highlight=organelles,membranes&rid=mcb.section.203
#210
3. Chap 1.4 The Life Cycle of Cells
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?highlight=cell%20cycle&rid=mcb.section.213#214
4. Chap 1.5 Cells into Tissues
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?highlight=tissues,cells&rid=mcb.section.222
5. Chap 1.6 Mol. Cell Biol—An Integrated View of Cells at Work
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?highlight=cell%20structure&rid=mcb.section.228
6. Chap. 2.0 Intro
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?highlight=molecules,biological&rid=mcb.chapter.145
7. Chap 2.1 Covalent Bonds
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?highlight=molecules,biological&rid=mcb.section.259#2
70
8. Chap 2.2 Non-Covalent Bonds
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?highlight=molecules,biological&rid=mcb.section.285#2
87
9. Chap 2.3 Chem. Equilibrium
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?highlight=molecules,biological&rid=mcb.section.316#3
19
10. Chap 2.4 Biochemical Energetics
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?highlight=molecules,biological&rid=mcb.section.335#3
45
11. Chap 2.5 Activation Energy and Reaction Rate
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?highlight=molecules,biochemical&rid=mcb.section.375
#380
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TENTATIVE LECTURE & LABORATORY SCHEDULE
BIOT 1315 Fall 2015
BIOT
1315
Falll 2013
week
#
LECTURE topic
Academic Cell
Biotechnology
LAB Instruction
topic
lab chap
Boyer 2000
reading
Ch 1a-c
LAB Exercise
BIOT 1315
Lab Manual
Ch 1a
water and other
solvents;
making buffers
making
buffers
#1 & #2;
H2O,
buffers &
pH
2.2-2.3
Ch 1
Dilutions;
Ch 1d-f, 2
buffers(comp)
& dilutions
#3;
dilutions
Labor Day
1.1-1.6
Ch 1
UV-vis spectrophoto-meter;
histology
Ch 2
polar vs. nonpolar solvent
interactions
Ch 1F; 2BD;
3ADEFGH;
4AB; 5A; 7AB
microscopic
viewing ;
separation of
molecules
based on
solubilities
#4; organic
extraction
assay of
proteins; use
of standard
curve
plots of
enzyme
kinetics
#7; prot
det. by
Bradford
method
#7; prot
det. by
Bradford
method
Boyer 2000 lab
exp manual
1
Aug.
18
2
Aug.
25
3
Sept.
1
4
Sept.
8
5
Sept
15
EXAM 1; amino
acids & protein
structure/function
EXAM 1
Ch 2
Spectroscopy
and assays
Ch. 2B, 5A
6
Sept.
22
enzymes kinetics;
Lineweaver-Burke
plots, i
2.2-2.3
Ch 2
enzyme kinetics
Ch. 5A
7
Sep.
29
enzyme inhibition;
carbohydrate
structure and
properties
2.2
inhibitor plots
Ch. 3ABCD
plot of
inhibitors
ex 5; kinetics of
Tyrosinase w/
inhibition
8
Oct.
6
lipids & membrane
structure
1.3
assay design &
purification flow
charts
Ch.
2B;5A;7ABC
Centrifugation
ex 5; kinetics of
Tyrosinase; ex
10; Isolation of
mito-chondria
ex 10; Isolation
of mito-chondria
cont.
9
Oct.
13
intro--scope of
biochem.; rev. of
atoms & bonds,
molec. Geometry;
classes of
biochemicals
water, pH, buffers
(Henderson-Hasselbalch equation);
pipettors; UV-vis
Spectrophotometers
NO CLASS MON.
WED: equilibrium;
cell
structure/function
amino acids &
protein
structure/function
Mol. Cell
Biology
chapter
2.0-2.1
Oct.
20
11
Oct.
27
12
Nov.
3
13
Nov.
10
14
Make solns.
For mito prep.
Isolation of
mitochondria;
assay of
mitochondrial
enzymes
centrifugation
Mitochondrial
structure; review
EXAM 2
10
histology
handout;
EXAM 2
nucleic acids;
replication,
transcription,
translation;
transformation by
plasmids
plasmid structure;
gel electrophoresis
Nutritional
Growth
Requirements
Ch 3
restriction
nucleases &
fragment sizes
Ch. 2C;
4ABC; 5AB
ex 5; kinetics of
Tyrosinase
(Prepare solns.)
assay of
mitochondrial
enzymes
Sterile
techniques
ex 10; Isolation
of mito-chondria
cont.
media
preparation and
bacterial
transformation
DNA isolation
and analysis
techniques
ex 14; DNA
isolation of
plasmid; DNA
gel
electrophoresis
ex 15;
Restriction
Digest of
plasmid DNA
ex 15;
Restriction
Digest of
plasmid DNA
Ch 3
DNA isolation
techniques
Use of
Restriction
Digests
Nov.
17
Ch 3
Genetic
engineering
Ch. 4B
Analysis of
gel results
15
Nov.
24
Ch 3
Genetic
engineering
Ch. 4B
Analysis of
gel results
16
Dec.
1
EXAM 3
review
EXAM 3
17
Dec.
8
FINAL EXAM
FINAL EXAM
ex 15;
Restriction
Digest of
plasmid DNA
Isolation of GFP
by HIC
chromatography
6