Course Syllabus for Microbiology (Biology 275)
Instructor: Nahel W. Awadallah
Office: Transfer Building, Rm# 220
Office Hours: Monday & Wednesday 010:30am - 12:30pm
Phone #: (910) 592-8081, Ext. 5511
Web Pages: & Http://
E-mail address: [email protected]
1. Course Prefix and No. BIO 275
Title: Microbiology
2. Credit Hours
Contact Hours: 8
Lecture Hours 4
Shop Hours
Clinical Hours 0
Lab Hours
3. Proficiency Exam
If YES, see Chairman of ________________________________
4. Prerequisite(s):
Course Prefix and No. BIO 110 or BIO 168
5. Corequisite(s):
Course Prefix and No. _________
Title: __________
Course Description:
This course covers principles of microbiology and the impact these organisms have on man and
the environment. Topics include the various groups of microorganisms, their structure,
physiology, genetics, microbial pathogenicity, infectious diseases, immunology, and selected
practical applications. Upon completion, students should be able to demonstrate knowledge and
skills including microscopy, aseptic technique, staining, culture methods, and identification of
microorganisms. This course has been approved to satisfy the Comprehensive Articulation
Agreement pre-major and/or elective course requirement.
Form CAC 2
7. Course Goals and Purposes:
The student will develop and demonstrate an understanding of the basic concepts of
8. Teaching Facilities and Resources:
Audio-visual Aids
Instructional Support Center
9. Number of Personnel Required
Lab Assistants 1
10. Required Textbooks and Workbooks:
1. Microbiology- Principles and Explorations by Jacquelyn G.
Black, 5th addition
2. Laboratory Exercises in Microbiology by Robert A. Pollack,
Walter Mondschein, Lorraine Findlay, and R. Ronald Modesto.
11. Required Clothing, Materials, Supplies, etc.
12. Attendance & Tardy Policy:
Attendance Policy
All students are expected to attend every scheduled class. Attendance is computed beginning
with the first scheduled class meeting. If an emergency prevents a student from attending class,
the student should notify his/her instructor as soon as possible.
In the event that a student does not attend at least 85 % percent of all scheduled contact hours,
the instructor may drop the student. If there are extenuating circumstances, the instructor has the
authority to make an exception to the attendance policy.
Students are expected to report to class on time. Specific guidelines regarding attendance and
tardiness are included in course outlines.
Tardy Policy
Students are expected to attend class on time and remain for the duration of the class. Late
arrivals an/or early departures will count toward total absences. Two tardies or two early
departures will count as one absence. Students whose persistent tardiness disrupts class
instruction may be dropped from the course at the discretion of the instructor.
13. Safety Rules:
All students must preview a slide presentation entitled "Laboratory Safety: Protecting Yourself
and Others"
Learn the proper escape routes in case of fire or explosion. Learn the location and operation of
the following safety equipment:
eye wash
safety shower
fire blanket
fire extinguisher
5. acid and base spill clean-up kit
6. fume hood
7. exhaust fan
14. Classroom, Shop, Clinical, or Lab Rules and Guidelines:
NO Smoking No Playing Around NO Eating or Drinking
Students with Disabilities
Students with documented disabilities will be afforded accommodations in their classes
consistent with the requirements of the Americans with Disabilities Act. You should notify your
instructor within the first week of classes if you have a disability for which you request
assistance. Additional information man be obtained from Student Support Services, in Warren
215, ext. 3511. Please understand that the college is unable to provide you any accommodation
unless you provide professional documentation of your disability and a timely notification of
your needs.
15. Methods of Student Evaluation (Examples: Lab Returns, Term Paper, Transcription, Exam, Timed
Writings, Pop Tests, Projects)
The final grade will be determined in accordance with the following scale:
90 - 100 = A
80 - 89 = B
70 - 79 = C
60 - 69 = D
0 - 59 = F
Inventories will be composed of objective and subjective questions.
16. Exit Criteria (Outcomes Assessment)
The student will develop and demonstrate an understanding of basic principles
underlying the science of microbiology. The cumulative grade average cumulative grade
average must be a minimum of 70 in order to earn the minimum passing grade of a C.
Final assessment will be based on the average of all grades received during the quarter.
17. Table of Contents (Course Content) (See Unit Titles)
Chapter 1 The scope and history of microbiology
Chapter 3 microscopy and staining
Chapter 4 Characteristics of Procaryotic & Eucaryotic Cells
Inventory I
Chapter 5 Essential concepts of Metabolism
Chapter 6 Microbial Growth and culture
Chapter 7 Microbial Genetics
Chapter 8 Gene transfer and genetic engineering
Inventory II
Chapter 9 Classification of Microorganisms
Chapter 10 VIRUSES
Chapter 11 Eukaryotic microorganisms and Parasites
Chapter 12 Sterilization and disinfections
Inventory III
Chapter 13 Antimicrobial therapy
Chapter 14 Host-microb interaction and Mechanisms of Pathogenicity
Chapter 15 Epidemiolgy and nosocomial infection
Chapter 16 Nonspecific Defenses of the Host
Chapter 17 Immunolgy I : Basic principles
Chapter 18 Immunolgy II: Practical Applications of Immunology
Chapter 19 diseases of the skin and eye; wounds and bites.
Chapter 20 Urogenital and sexually transmitted diseases
Inventory IV
Chapter 21 Diseases of the Respiratory System
Chapter 22 Oral and gastrointestinal diseases
Chapter 23 Cardiovascular, lymphatic and systemic diseases
Inventory V
Chapter 24 Diseases of the nervous system
Chapter 25 Environmental microbiology
Chapter 26 Applied microbiology
Inventory VI
Laboratory Exercises and Experiments:
1. The Microscope
2. Handling and Examing Cultures
3. The Handling Drop Preparation
4. Simple Strains
5. Gram Strain
6. Acid Fast Strain
8. Culture Media
9. Pure Culture Technique
10. Pour Plate and Subculture Techniques
11. Culturing Microorganisms from the Environment
12. Moist and Dry Heat
13. The Autoclave
14. Disinfectants
15. Antibiotics (Antibiotic Susceptibility Testing)
16. Primary Media for Isolation of Microorganisms
17. Some Metabolic Activities of Bacteria
18. Activities of Bacterial Enzymes
19. Streptococci Pneumococci, Enterococci
20. Staphylococci
23. Clinical Specimens from the Respiratory Tract
25. Clinical Specimens from the Intestinal Tract
26. Urine Culture Specimens
27. Neisseria and Spirochetes
28. Anaerobic Bacteria
18. Course Content Outline and Behavioral Objectives
See Attached
19. Class Handouts
20. Supplemental Bibliography of reference materials and audio visual aids used in this course.
The student should be able to:
As a result of the experiences provided by the lectures and experiments in this course
students will have the opportunity to accomplish successfully following objectives.
1. Define microorganisms.
2. Enumerate the characteristics of prokaxyotic
and eukaryotic cells.
3. Label a compound microscope and list the functions of each part.
4. List the main types of laboratory equipment
and how each is employed in a microbiology
5. Differentiate between positive and negative
stained organisms.
6. List the procedure for simple and gram
7. List the major types of culture media.
8. List the three major morphological shapes
of bacteria.
9. Draw and label a flagella.
10. Draw and label a cell wall.
11. List the stages of endospore formation.
12. List the components of DNA.
13. Define transcription and translation.
14. Define mutation processes.
15. Differentiate between transformation, transduction and conjugation.
16. Define and give examples of genetic engineering and recombinant DNA.
17. List the characteristics and culture techniques of Rickettsial, Chlamigdial, Mycoplasmas,
Bdellovibios, and Actinomycetes.
18. Enumerate virus characteristics.
19. List the stages of lytic and lysogenic cycles.
20. List the concepts of oncogenes in human cancer.
21. List the major groups of eukaryotic organisms.
22. Enumerate the major physical methods for the control of microorganisms.
23. Enumerate the major chemical agents for the control of microorganisms.
24. Diagram the major factors influencing nosocomial infections.
25. Enumerate the major types of chemotherapeutic agents and mode of action of each agent
on microbes.
26. Categorize the major kinds of infectious diseases.
27. Diagram invasive mechanisms of bacteria.
28. List examples of mechanical and chemical host resistance.
29. Diagram the process of phagocytosis and inflammation.
30. Define interferons role in resistance.
31. List the properties of antigens and antibodies.
32. Differentiate B cells and T cells.
33. Differentiate active and passive immunity.
34. Define delayed-type hypersensitivity.
35. List the major types of amteseria and vaccines.
36. Define epidemiology.
37. Enumerate the normal flora of the human body.
38. Enumerate the major types of bacteria that enter the body via the respiratory tract.
39. Enumerate the viruses, chlamydiae, and Rickettsiae microbes that enter the body
via the respiratory tract.
40. List the pathogens associated with gastrointestinal disease.
41. Enumerate the pathogens that enter the body via the genitourinary tract.
42. Enumerate the pathogens that enter the body via the skin.
43. Enumerate the pathogens that enter the body via anthropod bites.

Tardy Policy - Johnston Community College