Speedy Upgrade Action
Order Form
Date: 08/03/2016
Purchase Order Number:
Eurogentec Quotation Number:
VAT Number:
(if VAT exempt please attach a copy of the certificate)
SHIPPING DETAILS
INVOICING DETAILS
Contact Person*:
Institute/Company*:
Department/Lab:
Address*:
Contact Person:
Institute/Company:
Department/Lab:
Address:
Post Code*:
City*:
Country*:
Tel*:
Fax*:
Email*:
NB: Incomplete forms could result in delays
Post Code:
Country:
Tel:
Fax:
Email:
City:
PROGRAMME NAME:
For a provided antigen programme, please provide information below
Type of antigen*: Select one
Total amount of antigen shipped*:
In Solution. Number of tubes:
Lyophilised. Number of tubes:
Buffer composition:
mg
Required storage temperature of antigen*
°C
Each tube contains
mg/ml of antigen in
ml buffer.
Each tube contains
mg of antigen to solubilise in
ml buffer.
Use all of the available antigen
Use
µg / injection / host
For an anti-peptide programme, please provide information below
Peptide Sequence Information (for Double X, please provide 2 sequences)
1. Peptide #1:
a. Peptide contains the protein’s
first AA (N-terminus)
last AA (C-terminus)
b. Design completed by
Eurogentec
Customer
Previously published
2. Peptide #2:
a. Peptide contains the protein’s
first AA (N-terminus)
last AA (C-terminus)
b. Design completed by
Eurogentec
Customer
Previously published
Neither
Neither
Immunisation Protocol
Standard package
Select A Standard Protocol
Yes, I want to upgrade this programme to the Speedy protocol
Carrier Protein Selection
KLH
OVA
BSA
THY
MAP8
Other:
Additional Services (Charges Apply)
Screen pre-immune serum
None
Type of host: Select
ELISA test None.
Total IgG Purification None
Affinity Specific IgG Purification NoneNumber of purifications to run
NB: If prior to the purification you do not indicate which serum to use, the purifications will be performed on the serum giving the best
ELISA results if available, otherwise the serum from each host will be pooled prior to purification.
Comments:
Eurogentec SA • Liege Science Park • Rue du Bois Saint-Jean 5 • B-4102 Seraing • Belgium • Tel +32 4 372 76 65 • Fax +32 4 264 07 88
RPM Liège • VAT (BE) 0427.348.346 • info@eurogentec.com • order@eurogentec.com • www.eurogentec.com
Speedy Upgrade Action
Order Form
Animal Welfare Statement: I certify that the antigen is not dangerous for people or animals. The sample does not contain HIV or
Hepatitis virus or purified proteins from such viruses. Recombinant proteins from human viruses will be accepted only if the production
conditions are described. I certify that the antigen does not contain sodium azide.
Signed ___________________________________________ Date: _____________________________
Instructions for anti-peptide protocol orders:
1. Peptide design  please send your protein sequence to peptide.design@eurogentec.com
2. Your peptide design will arrive in 2-3 working days, copy these sequences in the appropriate box above
3. Email this form to order@eurogentec.com with attention Immunisation Department in the subject line
4. For any further questions please contact us info@eurogentec.com
Required amount of antigen / max. volume
Size of antigen:
Type of animal
Rabbit
Guinea Pig
> 18-20 kDa
amount of antigen
20 - 200µg
10 50µg
Size of antigen:
Type of animal
Rabbit
Guinea Pig
per injection and animal
recommended amount of antigen max. volume (solution or gel piece)
100µg
500µl
30µg
125-150µl
< 18-20 kDa
amount of antigen
40 - 400µg
20 - 150µg
per injection and animal
recommended amount of antigen
max. volume (solution or gel piece)
200µg
500µl
50µg
125-150µl
Antigen format / Shipment:
Format
Shipment
Lyophilised
at room temperature
Solution
on dry ice
Remarks
if possible no urea (max. 6M), acetic acid, guanidine hydro chloride, ..
Expected serum volume for the Speedy protocol
Species
pre-immune bleed
medium bleed
Rabbit
2ml
10ml
Guinea Pig
1ml
1 - 2ml
final bleed
50 - 70ml
3 - 5ml
Speedy immunization protocols
Day
Injection
Bleed
0
1.
pre-immune
7
2.
10
3.
18
4.
21
28
medium
final
ELISA testing
ELISA tests are for titre determination in crude or purified serum (100µg peptide required):
AS-ELIS-01
(1 plate, 1 peptide, testing of pre-immune-serum, 1. & 2. test bleed from 1 animal)
AS-ELIS-LL
(1 plate, 1 peptide, testing of pre-immune-serum & 2. test bleed from 2 animals)
AS-ELIS-LLDX
(2 plates, 1 peptide/plate, testing of pre-immune-serum & 2. test bleed from 2 animals)
Purification
For removing unspecific antibodies the serum can be purified by IgG- or affinity purification.
AS-PURI-SM
( 5ml serum [ 5mg peptide required], no QC)
AS-PURI-MED
(20ml serum [ 8mg peptide required], QC by SDS-PAGE)
AS-PURI-01
(50ml serum [10mg peptide required], QC by ELISA, SDS-PAGE, conc. determination)
Contact / Shipping address:
EUROGENTEC S.A. • Immunization Department • LIEGE Science Park • 4102 SERAING • Belgium • Tel.: +32 4 372 7400 • Fax: 32 4 372 7500
Web
Product information:
www.eurogentec.com
info@eurogentec.com
Eurogentec SA • Liege Science Park • Rue du Bois Saint-Jean 5 • B-4102 Seraing • Belgium • Tel +32 4 372 76 65 • Fax +32 4 264 07 88
RPM Liège • VAT (BE) 0427.348.346 • info@eurogentec.com • order@eurogentec.com • www.eurogentec.com
Speedy Upgrade Action
Order Form
Orders:
For a peptide design:
For ongoing programme information:
For latest offerings:
order@eurogentec.com Order-Fax: +32 4 264 0788
peptide.design@eurogentec.com
antibodies@eurogentec.com
www.eurogentec.com/proteomics.html
Eurogentec SA • Liege Science Park • Rue du Bois Saint-Jean 5 • B-4102 Seraing • Belgium • Tel +32 4 372 76 65 • Fax +32 4 264 07 88
RPM Liège • VAT (BE) 0427.348.346 • info@eurogentec.com • order@eurogentec.com • www.eurogentec.com