CURRICULUM VITAE
March 8, 2016
Personal Details
Name:
Levi A. Gheber
Date and place of birth:
May 14th, 1962, Romania
Immigration to ISRAEL:
August, 1974
Military Service:
1981-1985, I.A.F. - Electronics teacher
Family status:
Married, three children
Home address:
35 Patenkin Dan St., Beer-Sheva, Israel
Work address:
The Institute for Applied Biosciences / Department
of Biotechnology Engineering
Ben-Gurion University of the Negev
P.O.Box 653
Beer-Sheva, 84105
ISRAEL
Phone: +972-8-646-1904
Fax: +972-8-647-7188
email: glevi@bgumail.bgu.ac.il
Education
1980-1981
Electronics & Microcomputers technician, Technical College, Tel-Aviv
1985-1988
B.Sc. Physics , Ben-Gurion University of the Negev, Beer-Sheva, Israel
1988-1990
M.Sc. Physics of condensed matter, Ben-Gurion University
of the Negev, Beer-Sheva, Israel. Title of thesis:
"Investigation of metal islands by means of STM", advisors
Prof. V. Volterra and Prof. G.Gorodetsky.
1990-1995
Ph. D. Physics of condensed matter, Ben-Gurion University
of the Negev, Beer-Sheva, Israel. Title of thesis:
"Characterization of metal- semiconductor systems with
STM" advisors Prof. V. Volterra and Prof. G. Gorodetsky.
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Employment
1999-
Lecturer, Department of Biotechnology Engineering and
Institute for Applied Biosciences, Ben-Gurion University of
the Negev, Beer-Sheva, Israel.
1998-1999
Associate Research Scientist, Biology Department, Johns
Hopkins University, Baltimore, MD, USA.
1995-1998
Postdoctoral Research Assistant, Biology Department,
Johns Hopkins University, Baltimore, MD, USA.
1990-1995
Research Assistant / Instructor, Department of Physics,
Ben-Gurion University of the Negev, Beer-Sheva, Israel.
1988-1990
Research / Teaching Assistant, Department of Physics,
Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Teaching Experience
1.
Physics laboratory for first year undergraduate physics students.
2.
Physics laboratory for second year undergraduate physics students.
3.
Advanced Physics laboratory for third year undergraduate physics students.
4.
Course in classical mechanics for physicists.
5.
Course in thermodynamics for physicists.
6.
Course in statistical mechanics for physicists.
7.
Course in Material and Energy Balances for Biotechnology Engineering.
8.
Course in Thermodynamics for Biotechnology Engineering.
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Publications
1. Gheber, L. A., Gorodetsky, G. and Volterra, V. (1994). Studies of sub-micron
gold islands on silicon by STM. Thin Solid Films 238, 1-3.
2. Hershfinkel, M., Gheber, L. A., Volterra, V., Hutchinson, J. L., Margulis, L.
and Tenne, R. (1994). Nested polyhedra of MX2 (M=W, Mo; X=S, Se) probed by
High-Resolution Electron Microscopy and Scanning Tunnelling Microscopy.
Journal of American Chemical Society 116, 1914-1917.
3. Gheber, L. A., Hershfinkel, M., Gorodetsky, G. and Volterra, V. (1996).
Scanning tunneling spectroscopy studies of the Au-H- terminated Si interface.
Applied Physics Letters 69, 400-2.
4. Gheber, L.A., Edidin, M. (1997). Plasma membrane domains may be created
by vesicle traffic to and from the cell surface. Molecular Biology of the Cell 8,
457.
5. Hershfinkel, M., Gheber, L. A., Homyonfer, M., Tenne, R. and Volterra, V.
(1997). Surface modifications: nanostructures and nested polyhedra generated by
pulsing the STM tip. Surface Review and Letters 4, 1015-20.
6. Edidin, M., Kenworthy, A. K. and Gheber, L. A. (1998). Light microscopy
beyond the wavelength limit: methods for characterizing cell surface membranes.
Microscopy and Microanalysis 4, 1018-1019.
7. Hwang, J., Gheber, L. A., Margolis, L. and Edidin, M. (1998). Domains in Cell
Plasma Membranes Investigated by Near-field Scanning Optical Microscopy.
Biophysical Journal 74, 2184-2190.
8. Gheber, L. A., Hershfinkel, M., Gorodetsky, G. and Volterra, V. (1998).
Investigation of the composition and electrical properties of gold-H-terminated
silicon(111) interface. Thin Solid Films 320, 228-235.
9. Gheber, L. A., Hwang, J. and Edidin, M. (1998). Design and Optimization of a
Near-field Scanning Optical Microscope for Imaging Biological Samples in
Liquid. Applied Optics 37, 3574-3581.
10. Hsu, K. and Gheber, L. A. (1999). Tip-sample interaction in a "shear-force"
near-field scanning optical microscope. Review of Scientific Instruments 70, 36093613.
11. Gheber, L. A. and Edidin, M. (1999). A model for membrane patchiness:
Lateral diffusion in the presence of barriers and vesicle traffic. Biophysical
Journal 77, 3163-3175.
12. Taha, H., Marks, R.S., Gheber, L.A., Sukenik, C., Rousso, I., Newman, J.,
Lewis, A. (2003). Protein printing with an atomic force sensing nanofountainpen..
Applied Physics Letters 83, 1041-1043.
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Presentations
-L.A. Gheber, G. Gorodetsky and V. Volterra: "Studies of Submicron Gold Islands on Silicon by
STM", STM '91 International Conference on STM, Interlaken, August 12-16, 1991.
-L.A. Gheber, M. Hershfinkel, G. Gorodetsky and V. Volterra: "Surface Modification of WSe 2
by STM", NANO II Second International Conference on Nanometer Scale Science and
Technology, Moscow, August 2-6, 1993.
-M. Hershfinkel, L.A. Gheber, G. Gorodetsky and V. Volterra: "Scanning Tunneling Microscopy
of Polyhedral Structures", NANO II Second International Conference on Nanometer Scale
Science and Technology, Moscow, August 2-6, 1993.
-L.A. Gheber, M. Hershfinkel, G. Gorodetsky and V. Volterra: "Surface Modification of WSe2
by STM", 27th Annual Meeting of the Israel Society for Microscopy, Rehovot, May 23-24, 1993.
-M. Hershfinkel, L.A. Gheber, V. Volterra and G. Gorodetsky: "Scanning Tunneling Microscopy
of Polyhedral Structures on MoS2", 27th Annual Meeting of the Israel Society for Microscopy,
Rehovot, May 23-24, 1993.
-V. Volterra, L.A. Gheber and M. Hershfinkel: "Scanning Tunneling Microscope Studies of
Semiconductors in Ambient Conditions", 27th Annual Meeting of the Israel Society for
Microscopy, Rehovot, May 23-24, 1993.
-L.A. Gheber, M. Hershfinkel, V. Volterra and G. Gorodetsky: "A STS Study of the Au/Si
Interface", Symposium on Surfaces: Characterization and Dynamics, The Weizmann Inst.,
Rehovot, Jan 14, 1996.
-M. Hershfinkel, L. A. Gheber, M. Homyonfer, R. Tenne and V. Volterra: "Inducing NanoStructures and Nested Polyhedra with the STM Tip", The Israel Physical Society meeting,
Jerusalem, April 1, 1996.
-M. Hershfinkel, L. A. Gheber, M. Homyonfer, R. Tenne and V. Volterra: "Surface
Modifications: Nano-Structures and Nested Polyhedra Generated by Pulsing the STM Tip",
ICSOS-5 in France, July 8-12, 1996.
- L. A. Gheber, J. Hwang and M. Edidin: "Imaging Rough Biological Samples in Liquid with
Fluorescence Near Field Scanning Optical Microscope", Scanning Microscopy 1996 meeting,
Bethesda, MD, USA, May 11-16, 1996
- L. A. Gheber, J. Hwang and M. Edidin: "High Resolution Imaging of Cell Membranes in
Liquid Using a Fluorescence Near Field Scanning Optical Microscope", 6th International
Congress on Cell Biology & 36th ASCB meeting, San Francisco, CA, USA, December 7-11,
1996.
-L. A. Gheber, J. Hwang and M. Edidin: "Fluorescence and Morphological Imaging of Cells in
Liquid Using a Near Field Scanning Optical Microscope", NFO-4, Jerusalem, Israel, 1996.
-L. A. Gheber and M. Edidin: "Plasma membrane domains may be created by vesicle traffic to
and from the cell surface". 37th ASCB annual meeting, Washington, DC, USA, December 13-17,
1997.
-L.A. Gheber and M. Edidin: "Vesicle traffic and barriers to lateral mobility create microdomains in the plasma membrane - a simulation". Biophysical Society 42nd Annual Meeting,
February 22-26, 1998, Kansas City, Missouri, USA
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-L.A. Gheber and M. Edidin: "Tip-sample interaction in NSOM". SXM 3, 3rd Conference on
development and Technological Application of Scanning Probe Methods, September 14 –17,
1998, Basel Switzerland.
-L.A. Gheber: "Imaging Biological specimens in liquid with a Near-field Scanning Optical
Microscope (NSOM)", The Israeli Vacuum Society meeting, The Weizmann Institute, Rehovot,
Israel, 1999
-L.A. Gheber "Organization of plasma membrane proteins investigated with
Near-field Scanning Optical Microscopy (NSOM)" . The 6th Minerva Winter School, February
27, 2000, The Weizmann Institute, Rehovot, Israel
-L.A. Gheber "Clusters of membrane proteins revealed with Near-field Scanning Optical
Microscopy (NSOM)", Israel Physical Society 46th Annual Meeting, May 11th, 2000, Technion,
Haifa, Israel
-L.A. Gheber "Clusters of membrane proteins revealed with Near-field Scanning Optical
Microscopy (NSOM)", MWW2000 workshop, October 10th ,2000, Wuppertal, Germany.
-L.A. Gheber "Clusters of membrane proteins revealed with near-field scanning optical
microscopy (NSOM)", 2nd France-Israel Workshop on Bioanalytical Sensors, Biochips
and Nanobiotechnology, December 11th , 2000, Autrans, France.
-L.A. Gheber “Near-field Optics: Not just imaging”, seminar at Electrical Engineering Dept.,
Tel-Aviv University. November 29th, 2001.
-L.A. Gheber “High-resolution imaging and structuring with light in near-field”, seminar at the
Nanoscience Center, Tel-Aviv University. December 26th, 2001.
-L.A. Gheber “High-resolution imaging and structuring with light in near-field”, seminar at the
Physics Department, Ben-Gurion University, January 15th, 2002.
-L.A. Gheber “Near-Field Optics: Imaging and Structuring Surfaces with Nano-Resolution”, The
67th Annual meeting of the Israel Chemical Society, Jerusalem, January 29-30, 2002
-Zvi Teff, Zvi Priel and Levi A. Gheber, “Direct Measurement of Forces Applied by Cilia”, 21st
Israel Vacuum Society General Conference, Sept. 11, 2002
-Eran I. Oz and Levi A. Gheber, “Automatic analysis of nanometer-size membrane protein
clusters”, 21st Israel Vacuum Society General Conference, Sept. 11, 2002
-Yael Lavi and Levi A. Gheber, “Dynamic nano-clusters on cell membranes”, 21st Israel
Vacuum Society General Conference, Sept. 11, 2002
-Rodica M. Ionescu, Robert S. Marks, Levi A. Gheber, “Enzyme nanolithography”, 21st Israel
Vacuum Society General Conference, Sept. 11, 2002
-Hesham Taha, Robert S. Marks, Levi A. Gheber and Aaron Lewis, “Protein Printing with a
NanoFountainPen”, 21st Israel Vacuum Society General Conference, Sept. 11, 2002
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-Rodica Ionescu, Robert Marks, Levi Gheber, “Enzyme nanolithography”, Bi-national FranceIsraeli Symposium on Enabling Nano- and Micro-Technologies in Biotechnology, Beer-Sheva,
Oct.31, Israel 2002
-L.A. Gheber “High-resolution imaging and structuring with light in near-field”, seminar at the
Physics Department, Nuclear Research Center – Dimona, February, 2003.
-Rodica Ionescu, Robert Marks, Levi Gheber, “Modulation of nano-depressions in proteinaceous
substrates by enzyme nanolithography”, Nano-biotechnologies II, Grenoble, April 22-24, France
2003
-Rodica Ionescu, Robert Marks, Levi Gheber, “Enzyme nano-lithography”, Workshop on NanoElectronics-The Cross Road of Multi-Disciplines, Haifa, May 22, Israel 2003
-Rodica Ionescu, Robert Marks, Levi Gheber, “Enzyme nano-lithography”, Gordon Research
Conferences-Chemical Sensors & Interfacial Design, Salve Regina University, Newport, Rhode
Island, August 3-8, USA 2003
Grants
- ISF - Equipment for the laboratory of a new researcher, 2000
- ISF - "Biochemical Nanophotolithography", 2000 - 2004 (with Dr. Robert Marks)
- BSF - "Dynamic Clusters of MHC-I on the plasma membrane", 2000-2002 (with Prof. Michael
Edidin, Johns Hopkins University)
- Ministry of Science “Infrastructure” – “NSOM Nano-Arrayed Biochips”, 2001-2004,
Coordinator (with Dr. Robert Marks and Prof. Aaron Lewis – HUJI)
- European Commission, Fifth framework Programme, “MENDOS” (Biomimetic Optical
sensors for Environmental Endocrine Disruptor Screening), 2003-2005.
Research interests
Patterning and imaging of (bio)surfaces with molecular resolution, using Scanning Probe
Microscopy (SPM).
Biophysical methods, advanced microscopical/optical techniques.
Bio-electronics (bio-chips, lab-on-a-chip, nano-fluidics).
Organization of cell membranes.
Digital image analysis and processing.
Abstracts of current research
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Biochemical nano-photolithography : The Near-field Scanning Optical Microscope (NSOM), used in this
project, employs a tapered optical fiber probe to illuminate an area of ~ 100 nm diameter of the sample.
Using the light emerging from the NSOM probe, photochemical reactions are induced, which enable us to
bind proteins from the solution to the surface and/or break chemical bonds and release the photo-removable
portion from areas of an immobilized organic film, conjugated to the substrate.
Using an AFM or NSOM probe functionalized with a proteolytic enzyme, we are attempting nanoengraving of a protein-coated surface. As an alternative approach, we are engraving wells and channels of
nanometer scale by delivering a proteolytic enzyme via a nano-pipette, controlled by a SPM (Nano
Fountainpen) to a protein layer.
Dynamic clusters of MHC-I on plasma membrane of living cells : We have directly imaged clusters of
HLA class I molecules labeled with fluorescent antibody, using a Near-field Scanning Optical Microscope
(NSOM); the patches measure ~300nm-600nm in diameter. Subsequently, we have proposed a model,
illustrated by a computer simulation, which explains the mechanism for the formation of such patches, as a
combination of lateral diffusion, barriers to lateral diffusion and vesicle traffic to and from the plasma
membrane. Presently we are studying the dynamics of these clusters in living cells expressing GFP-tagged
MHC class I proteins, with total internal reflection fluorescence microscopy (TIRFM) and NSOM.
Direct measurement of forces from cilia: Cilia, one of the first motors in nature, are hair-like protrusions
from the cell surface, which perform a periodic motion, designed to either move the cell itself (swimming)
or propel liquids around the cell. Mucus propelling cilia are some 5 microns long, 0.25 in diameter, closely
packed (~ 1 micron interciliary distance) and they beat in a coordinated manner, to form a spatial wave.
The motion is facilitated by molecular motor proteins (dyenin), moving pairs of microtubules.
We are studying the ciliary motion by contacting an active ciliary area with the probe of AFM/NSOM and
directly measuring the forces applied to the tip by the beating cilia.
Scientific activity
-Board member (treasurer), The Israel Vacuum Society (IVS) 1999 - 2002
-Organizing committee, The 34th Annual Conference, Israel Society for Microscopy, May
18th 2000, Beer-Sheva, Israel.
-Organizing committee, 2nd France-Israel Workshop on Bioanalytical Sensors, Biochips
and Nanobiotechnology, December 11-15, 2000, Centre MAEVA, Autrans, France
-Organizing committee, Bi-Institutional Symposium on Glycobiology, October 15, 2001,
Beer-Sheva, Israel.
-Member, The University Commission for Computing Policy
-Member, The Ilse Katz Center for Meso and Nanoscale Science and Technology.
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Last update 16:15 05/08/2003
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