General Microbiology: 3340: 03/04
Fall 2008
Kennesaw State University
Department of Biological & Physical Sciences
Spring 2011
Fundamental Microbiology
Premila Achar
Lecture: Clendenin Building- Rm 1008
Lab: Prillaman Health Sciences Building- Rm 3413
Office phone:
Office Hours:
BIOL 2261: Lecture M & W : 9:30 AM -10:45 AM
Lab M & W
: 11:30 AM-1:30 PM
Lab W
: 2:00 PM- 4:00 PM
M & W 2:30-3:30 pm
[email protected]
Catalog Description: BIOL 2261; Fundamental Microbiology. 3-2-4.
Course Requirement and Prerequisite: “C” or better grade in BIOL 2221/2221L. Basic principles
and techniques of microbiology including the various types of microbes, their morphology, metabolic
processes and their relationships to humans; basic microbiology laboratory principles emphasizing
fundamental isolation, identification, and culture techniques. Primarily for nursing majors. Cannot be
used for credit toward a degree in Biology.
Biology 2261 is a fundamental microbiology course that fulfills the microbiology requirement for
nursing majors at Kennesaw State University. Biology 2261 CANNOT be used to satisfy the General
Education Science Requirements, CPC deficiencies, nor can it be used towards the BS in Biology,
Biotechnology, Chemistry, or Biochemistry.
Learning Outcomes:
1. To understand what microorganisms are, and their roles in nature.
2. To understand the cell structure, metabolic processes, and growth of microorganisms.
3. To understand the applied uses of microorganisms.
4. To master the basic skills of handling, examining, and identifying microorganisms.
5. To investigate cell structure, metabolic processes, and growth of microbes using lab experiments.
6. To investigate the applied uses of microorganisms using lab experiments.
7. To fulfill the lecture and lab requirements of this course at an average level or above.
Text: Microbiology: A Clinical
Authors: Anthony J.
Strelkauskas, Jennifer E.
Strelkauskas, Danielle MoszykStrelkauskas
Laboratory Manual:
Microbiology: Laboratory Theory
and Application, 3rd Ed.
Authors: Michael J. Leboffe,
Burton E. Pierce.
General Lecture Policies:
 Attendance is required for periods in which an invited speaker or student presentation is
 Participation in class activities will results in bonus points to earn extra credit. Class activities are
not scheduled.
 If you miss a lecture, YOU are responsible for obtaining all notes, announcements, and
 Lecture notes will be available via my Home page:
 The lecture schedule provided is TENTATIVE and subject to changes. Students will be notified
of schedule changes as soon as possible in class or will be posted on the instructor’s web site
under “notices”.
 Cellular telephones, pagers and other similar devices must be turned off during class.
 No snacking is allowed in the class room once lecture begins.
 If you miss class, it is your responsibility to get any missed notes and handout from another
student. Instructor’s Power point lectures/quiz/exam samples will be posted on instructor’s
web page.
General Laboratory Policies:
Each student should have the lab manual at the beginning of each lab (you will be denied lab
acess if you don’t have the lab manual on the first day of lab).
Safety glasses, purchased by each student, are required to be worn during all exercises, failing
which you will lose you 10 points out of your grand total*** (N.B you are not guaranteed
the dept safety glasses since they “walk out” of lab mysteriously and if a student is seen
walking out with dept belongings, university rules will be applied to you).
Numbered microscope will be assigned at each student desk also numbered. Mishandling
microscope (leaving slide on stage, stains, oil on lenses etc) will result from first warning to
losing 5 points if ignored.
You must prepare for laboratory each week by reading the scheduled exercises in the laboratory
On days when new exercises begin, the laboratory period will begin with a pre-lab quiz based on
the laboratory manual. Please see the schedule below for the dates of lab exercises. Pre-Lab
quizzes are worth 10 points each.
Post lab Quizzes worth 10 points each based on completed lab exercises
The mixed unknowns lab report is worth 50 points ( refer my home page for guidlines)
Students will be notified of schedule changes as soon as possible in class.
Before leaving the laboratory, each student must have the instructor or laboratory assistant to
inspect the condition of his or her work area, microscope, and other equipment. Failure to
clean the work area and equipment or to return equipment to its proper place will result in a 1
point reduction in the lab report grade per offense.
Attendance Policy:
 You must attend the laboratory section in which you are registered.
 Attendance of leach lab session is essential for success in this class. Roll will be taken and this
will factor into the final course grade.
 You must plan to attend laboratory for the full period each week. Students who arrive in lab
late or leave lab before being dismissed may not receive credit for the lab.
 Each absence will result in a loss of 5 points** in the total grading policy (see grading policy
 Attendance is required for periods in which an invited speaker or student presentation is
 If you miss 3 lab sessions, YOU will be considered FAIL.
Lab Safety regulations:
Failure to obey safety regulations will result in a 10 points*** penalty per offense
Students are required to obey all safety regulations during laboratory,
In particular:
 No food or drink is allowed in the lab as per department and university regulations. This
includes unopened food packages and beverage containers.
Students are required to wear safety glasses. Students are encouraged to purchase their own
safety glasses.
Shorts and short skirts are prohibited.
Open-toed shoes, open-heeled shoes, heeled shoes, sandals, and bare feet are prohibited.
Long hair, immaterial of gender, should be securely tied up to avoid contact with the Bunsen
All waste must be disposed in properly labeled containers as directed by the instructor or lab
After each exercise, materials and glasswears to be moved to assigned places in lab ( tubes,
plates, pipettes, microtips, slides, cover slips, scotton swab, gloves and related materials)
Cellular telephones, pagers and other similar devices must be turned off during lab session.
Assignment and Quizzes and Exams:
Cellular telephones, pagers and other similar devices must be turned off and kept in bags at all
times during quizzes and exams.
Evaluation will take the form of Assignments, Quizzes and Exams*
Several weeks identified on the schedule, the laboratory will begin with a laboratory pre quiz.
The quiz will contain questions about the new lab exercise that you are starting. Lab quizzes
graded (see course grading table).
Post lab quizzes are based on completed labs.
The lab worksheets pertaining to each lab is to be completed by each student since questions
will be selected from this section for lab post quizzes. Worksheet will be checked in labs for
The laboratory report on lab exercise, PRACTICUM/ “mixed unknowns”, is also graded. See
lab schedule below for due date for report and 5 points/day will be deducted until submitted.
All quizzes, assignments and other course materials are related to lab work not collected by
students will be shredded and recycled at the conclusion of the final exam period
Lab sessions will not be repeated for a particular group.
Make-up quiz/exam/assignments will only be granted in extreme circumstances, and at the
instructor’s discretion. Examples of acceptable excuses include: serious illness, funeral and
others. The determination of the validity of other excuses and the necessary documentation to
support them will be at the instructor’s discretion.
Make-up examinations may consist of different question types, including essay questions, and
they are usually more difficult than the scheduled examination.
There is no make-up for Final exam
There is no further make-up if student missed a scheduled make-up.
If dates in the schedule below clashes with an event you have planned prior to posting of
this syllabus onto my webpage, please inform me in class or via email before
DROP/ADD week to afford you a make-up.
The dates for examinations are listed below to be marked on your calendar and if it clashes
with any of your academic activities, student should notify me in the first week of lecture.
Inclement Weather:
In adverse weather situations, find out the status of school closings by checking the following
1. The grade in this class is determined by the total number of points earned on examinations,
laboratory quizzes, and the mixed unknown report ( Table 1).
2. Each exam will contain approximately 10 % laboratory material.
3. Examinations are closed book, multiple-choice exams. The material covered on each exam will be
reviewed in class. Exam questions include content from lectures, textbook readings, and laboratory
4. The final examination is comprehensive and counts for 100 points for each student in the class.
Final letter grades A, B, C…in the course will be assigned according to the point breakdown listed
Graded assignments
2 Mid-term Exams @ 100 pts*
Comprehensive Final Exam
1 class Activities @ 10 points
7 Pre-Lab Quizzes @ 10pts each
4 Post-Lab Quizzes @10pts each
Lab Unknown Report
Grand Total
Final course grades
Percentage (%)
200 pts.
100 pts.
10 pts
70 pts.
40 pts.
50 pts.
470 pts
o *Exams will include questions from lectures & labs
o **Failure to attend each lab will result in 5 points (see attendance policy for lab)
& absence for 3 labs is considered Fail in the course.
o ***Failure to obey safety regulations will result in a 10 points penalty per offense
(see lab safety regulations).
Tentative Lecture Outline (subject to change)
Jan 10
Book Chapters
Chapter 1
Introduction to class
Characteristics of Disease-Causing Microorganisms
Introduction Microbiology
Jan 12
Jan 17
Jan 19
Jan 24
Jan 26
MLK holiday
Chapter 9
Chapter 10
Chapter 12
Structure of Bacteria
Bacterial Growth
Structure of Viruses
Jan 31
Feb 2
Chapter 13
Chapter 14
Viral Pathogenesis
Parasitic and Fungal Infections
Disease Mechanisms and Control and Treatment
Feb 7
Chapter 5 & 6
Requirements for Infection &
Transmission of Diseases
Feb 9
Chapter 7
Principles of Disease
Feb 14
Chapter 8
Emerging/Re-emerging Infectious
Feb 16
Exam 1
Feb 21
Feb 23
Feb 28
March 2
Chapter 18
Chapter 19
Chapter 20
Chapter 20 (contd.)
Control of Microbial Growth
Antibiotic Resistance
Antibiotic Resistance
Microbial Infections
March 5-11
March 14
March 16
March 21
March 23
March 28
March 30
April 4
April 6
April 11
April 13
April 18
Spring break
Class Activity
Chapter 21
Chapter 21 (Contd.)
Chapter 22
Chapter 22 Contd.)
Chapter 23
Chapter 23 (Contd.)
Exam 2
Chapter 24
No Class- Library day
Chapter 25 and Chapter 26
Respiratory System
Digestive System
Genitourinary Systems
Central Nervous Systems
Deadly pathogens of Humans
Blood and Skin and Eyes
Chapter 25 and Chapter 26
Chapter 28
Chapter 17
April 20
April 20
April 25
April 27
Failures of the Immune response:
Revision Chapter 18 - Chapter
Last day of classes
Final Exam
May 2
TBA May 3-9
Laboratory Schedule
BIOL 2261 – Spring 2011
Week of Lab Dates
Jan 10
Jan 17
Jan 24
Lab Units
No labs
Lab Manual Exercise
No labs MLK holiday
Unit 1: Lab Safety &
Fundamental Skills
Unit 2: Microbial
Complete 1-1, 1-3, 1-4;
Begin 2-1, 2-2
2-1, 2-2
Feb 7
Unit 3: Control of
Complete labs 2-1, 2-2
Begin 2-12, 2-13, 2-14
2-12, 2-13,
Feb 14
Unit 4: Microscopy:
Viewing MedicallyImportant Microbes
Complete 2-12, 2-13, 2-14
Begin 3-1, 3-3, prepared
slides from Section 12:1-4
Jan 31
Feb 28
March 7
March 14
March 21
Unit 5: Staining I Gram
stain and endospore
No labs
Unit 5: Staining II:
Capsule and Endospore
Begin 1-1, 1-3, 1-4
Begin 3-7, 3-10
Practice streak plate
1-1, 1-3, 1-4
Unit 1 &
3-1, 3-3
3-7, 3-10
Spring Break
Complete 3-7, 3-10
3-14 (streak plate))
Gram stain and Endospore
Establish Pure Cultures of
Unit 3 &
Unit 4
March 28
Unit 6: Selective Media
with Clinical Specimen
Collection & Analysis
April 4
Complete Practicum
April 11
Unit 7: Antibacterial
Susceptibility Testing and
April 18
April 25
May 2
Unit 8: Quantitative
Indirect ELISA
No labs
Begin 4-3, 4-4, 4-5, 4-6, 5- 4-3, 4-4, 425, 5-30, 6-2, 7-1
5, 4-6, 5-25,
Inoculate G+ cocci and G- 5-30, 6-2, 71
bacilli unknowns onto
Selective Media
Complete 4-3, 4-4, 4-5, 46, 5-25, 5-30, 6-2, 7-1
Begin 5-24, 7-3, 7-5, 7-6
Complete 5-24, 7-3, 7-5, 76
11-7; ClearView Strep
Test (not in lab book)
Unit 5 &
Unit 6
5-24, 7-3, 7- Practicum
5, 7-6
due in
Unit 7 &
Unit 8
Last day of classes
Academic Integrity
Every KSU student is responsible for upholding the provisions of the Student Code of Conduct, as
published in the Undergraduate and Graduate Catalogs. Section II of the Student Code of Conduct
addresses the University's policy on academic honesty, including provisions regarding plagiarism and
cheating, unauthorized access to University materials, misrepresentation/falsification of University
records or academic work, malicious removal, retention, or destruction of library materials,
malicious/intentional misuse of computer facilities and/or services, and misuse of student identification
cards. Incidents of alleged academic misconduct will be handled through the established procedures of
the University Judiciary Program, which includes either an "informal" resolution by a faculty member,
resulting in a grade adjustment, or a formal hearing procedure, which may subject a student to the Code
of Conduct's minimum one semester suspension requirement.
Plagiarism and Cheating
The above is in accordance to the University policy concerning plagiarism and cheating as stated in the
KSU Student Code of Conduct. Refer to KSU document. All instances of academic misconduct, both
intentional and unintentional, will be handled through established procedures of the University Judiciary
Program ( Students violating the University's
academic honesty code are subject to University-level disciplinary action, which may include suspension
or expulsion from the University. For exams, any student observed cheating will receive an automatic
zero for the entire exam in addition to any penalties assessed through campus disciplinary procedures.
Academic Withdrawal Policy
Students may withdraw from one or more courses anytime before the last three weeks of the semester.
However, as of Fall 2004, students will be allowed a maximum of eight total withdrawals if they enter
KSU as a freshman. Transfer students will be allowed one withdrawal per fifteen credit hours
attempted, for a maximum of eight. Students who choose to pursue a second degree at KSU will be
allowed two additional withdrawals. Students who entered KSU before the Fall of 2004 will be allowed
one withdrawal per fifteen credit hours attempted for a maximum of eight. To withdraw, the student
should complete an official withdrawal form in the Office of the Registrar. Students who officially
withdraw from courses on or before the last day to withdraw without academic penalty will receive a
“W”. Students who officially withdraw after the last day to withdraw without academic penalty (and
before the last three weeks of the semester) will receive a “WF”, which will be counted as an “F” in
calculation of their grade point average.
The only exceptions to these withdrawal regulations will be for instances involving unusual
circumstances, which are fully documented. Students may appeal to the academic standing committee
for consideration of unusual circumstances.
Students cannot withdraw from their courses and remain in the program; they must carry at least 3
hours of credit. Program staff will not handle course withdrawals. Students must contact their home
institutions (where they are registered) and handle the process themselves. They must provide the
Faculty Member and Program Director with evidence that they have dropped the course.
Academic accommodations
Any student with a documented disability or medical condition needing academic accommodations of
class-related activities or schedules must contact the instructor immediately. Written verification from
the KSU disAbled Student Support Services is required. No requirements exist that accommodations be
made prior to completion of this approved University documentation. All discussions will remain
****Good Luck ****