Page 4
Limited Use Label License No. 41: BenchMark
™
Ladder
Protein
The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. The buyer may transfer information or materials made through the use of this product to a scientific collaborator, provided that such transfer is not for any Commercial Purpose, and that such collaborator agrees in writing (a) to not transfer such materials to any third party, and (b) to use such transferred materials and/or information solely for research and not for
Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. Invitrogen Corporation will not assert a claim against the buyer of infringement of patents owned by Invitrogen
Corporation and claiming this product based upon the manufacture, use or sale of a therapeutic, clinical diagnostic, vaccine or prophylactic product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. If the purchaser is not willing to accept the limitations of this limited use statement, Invitrogen is willing to accept return of the product with a full refund. For information on purchasing a license to this product for purposes other than research, contact Licensing Department,
Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, California 92008.
Phone (760) 603-7200. Fax (760) 602-6500.
©1997-2005 Invitrogen Corporation. All rights reserved.
BenchMark ™ Pre-Stained Protein Ladder
Catalog No: 10748-010
Lot No: 1283301
Size: 2 x 250
µ
Store at -20
°
C l
Introduction
The BenchMark ™ Pre-Stained Protein Ladder for SDS-PAGE allows easy visualization of protein molecular weight ranges during electrophoresis and evaluation of western transfer efficiency.
The important features of the standard are listed below:
•
Consists of 10 pre-stained protein bands in the range of 10-200 kDa
(proteins are covalently coupled to a blue or pink dye)
•
The fourth protein band from the top is coupled to a pink dye for easy orientation and to ensure proper identification of each protein
•
Supplied in a ready-to-use format
Note: Coupling of the chromophores to the proteins affects the apparent molecular weight of proteins in SDS-PAGE relative to unstained standards. Each pre-stained ladder is calibrated against unstained standards and the apparent molecular weight is reported (page 3). Use pre-stained protein ladders to determine the approximate molecular weight of proteins. For accurate molecular weight estimation of proteins, use unstained protein ladders.
Specifications
Contents: 2 x 250
µ l of BenchMark ™ Pre-Stained Protein Ladder
Storage Buffer: 50 mM Tris-HCl, pH 6.8; 5 mM EDTA; 10 mM DTT;
1% (w/v) SDS; 10% (w/v) glycerol
Storage:
Stability:
Store at -20°C
1 year at -20
Part No: 10748010.pps
°
C
Rev. Date: 19 July 2005
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
For technical support, contact tech_service@invitrogen.com.
Page 4
Limited Use Label License No. 41: BenchMark
™
Ladder
Protein
The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. The buyer may transfer information or materials made through the use of this product to a scientific collaborator, provided that such transfer is not for any Commercial Purpose, and that such collaborator agrees in writing (a) to not transfer such materials to any third party, and (b) to use such transferred materials and/or information solely for research and not for
Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. Invitrogen Corporation will not assert a claim against the buyer of infringement of patents owned by Invitrogen
Corporation and claiming this product based upon the manufacture, use or sale of a therapeutic, clinical diagnostic, vaccine or prophylactic product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. If the purchaser is not willing to accept the limitations of this limited use statement, Invitrogen is willing to accept return of the product with a full refund. For information on purchasing a license to this product for purposes other than research, contact Licensing Department,
Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, California 92008.
Phone (760) 603-7200. Fax (760) 602-6500.
©1997-2005 Invitrogen Corporation. All rights reserved.
BenchMark ™ Pre-Stained Protein Ladder
Catalog No: 10748-010
Lot No: 1283301
Size: 2 x 250
µ l
Store at -20
°
C
Introduction
The BenchMark ™ Pre-Stained Protein Ladder for SDS-PAGE allows easy visualization of protein molecular weight ranges during electrophoresis and evaluation of western transfer efficiency.
The important features of the standard are listed below:
•
Consists of 10 pre-stained protein bands in the range of 10-200 kDa
(proteins are covalently coupled to a blue or pink dye)
•
The fourth protein band from the top is coupled to a pink dye for easy orientation and to ensure proper identification of each protein
•
Supplied in a ready-to-use format
Note: Coupling of the chromophores to the proteins affects the apparent molecular weight of proteins in SDS-PAGE relative to unstained standards. Each pre-stained ladder is calibrated against unstained standards and the apparent molecular weight is reported (page 3). Use pre-stained protein ladders to determine the approximate molecular weight of proteins. For accurate molecular weight estimation of proteins, use unstained protein ladders.
Specifications
Contents: 2 x 250
µ l of BenchMark ™ Pre-Stained Protein Ladder
Storage Buffer: 50 mM Tris-HCl, pH 6.8; 5 mM EDTA; 10 mM DTT;
1% (w/v) SDS; 10% (w/v) glycerol
Storage:
Stability:
Store at -20°C
1 year at -20
°
C
Part No: 10748010.pps Rev. Date: 19 July 2005

Page 2
Directions
The BenchMark ™ Pre-Stained Protein Ladder is supplied in a ready-to-use format. There is no need to heat or add reducing agent.
1. Thaw the ladder at room temperature. Do not boil!
2. Vortex gently to ensure the solution is homogenous.
3. Load the ladder into the well of an SDS gel using the loading volumes listed below to obtain the best results:
Gel Electrophoresis Blotting
Standard Gel 20
µ l 10
Quality Control
BenchMark ™ Pre-Stained Protein Ladder must show 10 major bands when separated by electrophoresis on a Novex ® 4-20% Tris-Glycine gel.
Example
The apparent molecular weights of the protein bands in BenchMark ™
Pre-Stained Protein Ladder are shown below. Five microliters of
BenchMark ™ Pre-Stained Protein Ladder was separated on a Novex ®
4-20% Tris-Glycine gel.
Page 3
Apparent molecular weights were determined on a Novex ® 4-20%
Tris-Glycine gel by comparing the mobility of the BenchMark ™ Pre-Stained
Protein Ladder to the unstained BenchMark ™ Protein Ladder and aprotinin band (6 kDa) from Mark12 ™ Unstained Protein Standard.
Band No. Apparent Molecular Weight
1
2
5
6
3
4
7
8
9
10
*Orientation band (pink in color)
Page 2
Directions
The BenchMark ™ Pre-Stained Protein Ladder is supplied ready-to-use.
There is no need to heat or add reducing agent.
1. Thaw the ladder at room temperature. Do not boil!
2. Vortex gently to ensure the solution is homogenous.
3. Load the ladder into the well of an SDS gel using the loading volumes listed below to obtain the best results:
Gel Electrophoresis Blotting
Standard Gel 20
µ l 10
Quality Control
BenchMark ™ Pre-Stained Protein Ladder must show 10 major bands when separated by electrophoresis on a Novex ® 4-20% Tris-Glycine gel.
Page 3
Example
The apparent molecular weights of the protein bands in BenchMark ™
Pre-Stained Protein Ladder are shown below. Five microliters of
BenchMark ™ Pre-Stained Protein Ladder was separated on a Novex ® 4-20%
Tris-Glycine gel.
Apparent molecular weights were determined on a Novex ® 4-20%
Tris-Glycine gel by comparing the mobility of the BenchMark ™ Pre-Stained
Protein Ladder to the unstained BenchMark ™ Protein Ladder and aprotinin band (6 kDa) from Mark12 ™ Unstained Protein Standard.
Band No. Apparent Molecular Weight
1
2
5
6
3
4
7
8
9
10
*Orientation band (pink in color)

Page 2
Directions
The BenchMark ™ Pre-Stained Protein Ladder is supplied in a ready-to-use format. There is no need to heat or add reducing agent.
1. Thaw the ladder at room temperature. Do not boil!
2. Vortex gently to ensure the solution is homogenous.
3. Load the ladder into the well of an SDS gel using the loading volumes listed below to obtain the best results:
Gel Electrophoresis Blotting
Standard Gel 20
µ l 10
Quality Control
BenchMark ™ Pre-Stained Protein Ladder must show 10 major bands when separated by electrophoresis on a Novex ® 4-20% Tris-Glycine gel.
Example
The apparent molecular weights of the protein bands in BenchMark ™
Pre-Stained Protein Ladder are shown below. Five microliters of
BenchMark ™ Pre-Stained Protein Ladder was separated on a Novex ®
4-20% Tris-Glycine gel.
Page 3
Apparent molecular weights were determined on a Novex ® 4-20%
Tris-Glycine gel by comparing the mobility of the BenchMark ™ Pre-Stained
Protein Ladder to the unstained BenchMark ™ Protein Ladder and aprotinin band (6 kDa) from Mark12 ™ Unstained Protein Standard.
Band No. Apparent Molecular Weight
1
2
5
6
3
4
7
8
9
10
*Orientation band (pink in color)
Page 2
Directions
The BenchMark ™ Pre-Stained Protein Ladder is supplied ready-to-use.
There is no need to heat or add reducing agent.
1. Thaw the ladder at room temperature. Do not boil!
2. Vortex gently to ensure the solution is homogenous.
3. Load the ladder into the well of an SDS gel using the loading volumes listed below to obtain the best results:
Gel Electrophoresis Blotting
Standard Gel 20
µ l 10
Quality Control
BenchMark ™ Pre-Stained Protein Ladder must show 10 major bands when separated by electrophoresis on a Novex ® 4-20% Tris-Glycine gel.
Page 3
Example
The apparent molecular weights of the protein bands in BenchMark ™
Pre-Stained Protein Ladder are shown below. Five microliters of
BenchMark ™ Pre-Stained Protein Ladder was separated on a Novex ® 4-20%
Tris-Glycine gel.
Apparent molecular weights were determined on a Novex ® 4-20%
Tris-Glycine gel by comparing the mobility of the BenchMark ™ Pre-Stained
Protein Ladder to the unstained BenchMark ™ Protein Ladder and aprotinin band (6 kDa) from Mark12 ™ Unstained Protein Standard.
Band No. Apparent Molecular Weight
1
2
5
6
3
4
7
8
9
10
*Orientation band (pink in color)

Page 4
Limited Use Label License No. 41: BenchMark
™
Ladder
Protein
The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. The buyer may transfer information or materials made through the use of this product to a scientific collaborator, provided that such transfer is not for any Commercial Purpose, and that such collaborator agrees in writing (a) to not transfer such materials to any third party, and (b) to use such transferred materials and/or information solely for research and not for
Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. Invitrogen Corporation will not assert a claim against the buyer of infringement of patents owned by Invitrogen
Corporation and claiming this product based upon the manufacture, use or sale of a therapeutic, clinical diagnostic, vaccine or prophylactic product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. If the purchaser is not willing to accept the limitations of this limited use statement, Invitrogen is willing to accept return of the product with a full refund. For information on purchasing a license to this product for purposes other than research, contact Licensing Department,
Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, California 92008.
Phone (760) 603-7200. Fax (760) 602-6500.
©1997-2005 Invitrogen Corporation. All rights reserved.
BenchMark ™ Pre-Stained Protein Ladder
Catalog No: 10748-010
Lot No: 1283301
Size: 2 x 250
µ
Store at -20
°
C l
Introduction
The BenchMark ™ Pre-Stained Protein Ladder for SDS-PAGE allows easy visualization of protein molecular weight ranges during electrophoresis and evaluation of western transfer efficiency.
The important features of the standard are listed below:
•
Consists of 10 pre-stained protein bands in the range of 10-200 kDa
(proteins are covalently coupled to a blue or pink dye)
•
The fourth protein band from the top is coupled to a pink dye for easy orientation and to ensure proper identification of each protein
•
Supplied in a ready-to-use format
Note: Coupling of the chromophores to the proteins affects the apparent molecular weight of proteins in SDS-PAGE relative to unstained standards. Each pre-stained ladder is calibrated against unstained standards and the apparent molecular weight is reported (page 3). Use pre-stained protein ladders to determine the approximate molecular weight of proteins. For accurate molecular weight estimation of proteins, use unstained protein ladders.
Specifications
Contents: 2 x 250
µ l of BenchMark ™ Pre-Stained Protein Ladder
Storage Buffer: 50 mM Tris-HCl, pH 6.8; 5 mM EDTA; 10 mM DTT;
1% (w/v) SDS; 10% (w/v) glycerol
Storage:
Stability:
Store at -20°C
1 year at -20
Part No: 10748010.pps
°
C
Rev. Date: 19 July 2005
Page 4
Limited Use Label License No. 41: BenchMark
™
Ladder
Protein
The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. The buyer may transfer information or materials made through the use of this product to a scientific collaborator, provided that such transfer is not for any Commercial Purpose, and that such collaborator agrees in writing (a) to not transfer such materials to any third party, and (b) to use such transferred materials and/or information solely for research and not for
Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. Invitrogen Corporation will not assert a claim against the buyer of infringement of patents owned by Invitrogen
Corporation and claiming this product based upon the manufacture, use or sale of a therapeutic, clinical diagnostic, vaccine or prophylactic product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. If the purchaser is not willing to accept the limitations of this limited use statement, Invitrogen is willing to accept return of the product with a full refund. For information on purchasing a license to this product for purposes other than research, contact Licensing Department,
Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, California 92008.
Phone (760) 603-7200. Fax (760) 602-6500.
©1997-2005 Invitrogen Corporation. All rights reserved.
BenchMark ™ Pre-Stained Protein Ladder
Catalog No: 10748-010
Lot No: 1283301
Size: 2 x 250
µ l
Store at -20
°
C
Introduction
The BenchMark ™ Pre-Stained Protein Ladder for SDS-PAGE allows easy visualization of protein molecular weight ranges during electrophoresis and evaluation of western transfer efficiency.
The important features of the standard are listed below:
•
Consists of 10 pre-stained protein bands in the range of 10-200 kDa
(proteins are covalently coupled to a blue or pink dye)
•
The fourth protein band from the top is coupled to a pink dye for easy orientation and to ensure proper identification of each protein
•
Supplied in a ready-to-use format
Note: Coupling of the chromophores to the proteins affects the apparent molecular weight of proteins in SDS-PAGE relative to unstained standards. Each pre-stained ladder is calibrated against unstained standards and the apparent molecular weight is reported (page 3). Use pre-stained protein ladders to determine the approximate molecular weight of proteins. For accurate molecular weight estimation of proteins, use unstained protein ladders.
Specifications
Contents: 2 x 250
µ l of BenchMark ™ Pre-Stained Protein Ladder
Storage Buffer: 50 mM Tris-HCl, pH 6.8; 5 mM EDTA; 10 mM DTT;
1% (w/v) SDS; 10% (w/v) glycerol
Storage:
Stability:
Store at -20°C
1 year at -20
°
C
Part No: 10748010.pps Rev. Date: 19 July 2005